Protein Overview: AST2

General Information:
Name(s) found:	AST2 / YER101C [SGD]
Description(s) found: Found 25 descriptions. SHOW ALL	Protein that may have a role in targeting of plasma membrane [H+]ATPase (Pma1p) to the plasma membrane, as suggested by analysis of genetic interactions [SGD]
Organism:	Saccharomyces cerevisiae
Length:	430 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA ]
Biological Process:	biological_process [ND]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAEKILENKD PKLEAMTVDH EVSAPKPIPV DEPTLTRVAR PLRHVRHIPV KSLVFHSKHG  60
   61 PITFSYENKI KLPISKNKLV VQVNYVGLNP VDMKIRNGYT KPIYGEAGIG REYSGVITHV 120
  121 GDNLTNRWNV GDDVYGIYYH PKLAIGALQS SLLIDPRVDP ILMRPKNTLS PEKAAGSLFC 180
  181 LGTALNLLAQ LKEKDQLNTE SNVLINGGTS SVGMFAIQLL KRYYKVSKKL VVVTSGNGAA 240
  241 VLSEHFPDLK DEIIFINYLS CRGKSSKPLR RMLDTGKVVD YDDFNTLKET EDYTQGKFNV 300
  301 VLDFIGGYDI LSHSSSLIHA KGAYITTVGD YVGNYKKDVF DSWDNPSANA RKMFGSMLWS 360
  361 YDYSHFYFDP NIKIIPKKND WIHECGKLLN EGVVDCVVDK VYSWKNFKEA FSYMATQRAQ 420
  421 GKLIMKVEGF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		AAC3 AST2 ATG27 DUR3 ECM12 MON1 PRE2 PUF2 REH1 RPL29 RPL41A, RPL41B RPS30B, RPS30A RXT3 SFT1 SLX8 TKL2 TPS1 YDL133W YGR117C YGR149W YLR030W YLR326W YML6 YOR292C YPL199C

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SEC17	AST2	2	Unpublished Fields Lab Data
View Screen	DOT1	AST2	1	Unpublished Fields Lab Data
View Screen	SPT2	AST2	1	Unpublished Fields Lab Data
View Screen	DAS1	AST2	1	Unpublished Fields Lab Data
View Screen	RHO4	AST2	1	Unpublished Fields Lab Data
View Screen	UBC7	AST2	1	Unpublished Fields Lab Data
View Screen	CTF13	AST2	1	Unpublished Fields Lab Data
View Screen	ARG1	AST2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	AST2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..210] [359..430]	PSI-BLAST	125.0	Alcohol dehydrogenase
2	View Details	[211..358]	PSI-BLAST	125.0	Alcohol dehydrogenase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
oxidoreductase activity	4.14217990101505	bayes_pls_golite062009
oxidoreductase activity, acting on CH-OH group of donors	2.13774251017711	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	1.96417104841008	bayes_pls_golite062009
catalytic activity	1.79741889688267	bayes_pls_golite062009
alcohol dehydrogenase (NAD) activity	1.68027744358887	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor	1.22416351603098	bayes_pls_golite062009
chaperone binding	1.19079350615542	bayes_pls_golite062009
DNA binding	1.02274180655721	bayes_pls_golite062009
transcription regulator activity	0.955628854752953	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	0.905392379451071	bayes_pls_golite062009
nucleic acid binding	0.901776352952123	bayes_pls_golite062009
alcohol dehydrogenase activity, zinc-dependent	0.839863523675468	bayes_pls_golite062009
transporter activity	0.793769336118517	bayes_pls_golite062009
structural molecule activity	0.75267483298133	bayes_pls_golite062009
ligase activity	0.742884668104576	bayes_pls_golite062009
transmembrane transporter activity	0.635891484075352	bayes_pls_golite062009
substrate-specific transporter activity	0.555188550031886	bayes_pls_golite062009
binding	0.553666136934364	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	0.51503848775098	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.410307505174239	bayes_pls_golite062009
transcription factor activity	0.368443846770004	bayes_pls_golite062009
ion transmembrane transporter activity	0.29802814097478	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	0.261132005311306	bayes_pls_golite062009
active transmembrane transporter activity	0.239024094835991	bayes_pls_golite062009
cation transmembrane transporter activity	0.212092278332619	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.113340150368917	bayes_pls_golite062009
pyrophosphatase activity	0.0696058100530442	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.0591634687578807	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.0560606007580392	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2