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Protein Overview: TKL2

General Information:
Name(s) found: TKL2 / YBR117C [SGD]
Description(s) found:
Found 28 descriptions.
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  • Transketolase, similar to Tkl1p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 681 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    cytoplasm [IDALink to PubMed article]
    Biological Process: pentose-phosphate shunt [IGILink to PubMed article]
    Molecular Function: transketolase activity [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAQFSDIDKL AVSTLRLLSV DQVESAQSGH PGAPLGLAPV AHVIFKQLRC NPNNEHWINR  60
   61 DRFVLSNGHS CALLYSMLHL LGYDYSIEDL RQFRQVNSRT PGHPEFHSAG VEITSGPLGQ 120
  121 GISNAVGMAI AQANFAATYN EDGFPISDSY TFAIVGDGCL QEGVSSETSS LAGHLQLGNL 180
  181 ITFYDSNSIS IDGKTSYSFD EDVLKRYEAY GWEVMEVDKG DDDMESISSA LEKAKLSKDK 240
  241 PTIIKVTTTI GFGSLQQGTA GVHGSALKAD DVKQLKKRWG FDPNKSFVVP QEVYDYYKKT 300
  301 VVEPGQKLNE EWDRMFEEYK TKFPEKGKEL QRRLNGELPE GWEKHLPKFT PDDDALATRK 360
  361 TSQQVLTNMV QVLPELIGGS ADLTPSNLTR WEGAVDFQPP ITQLGNYAGR YIRYGVREHG 420
  421 MGAIMNGISA FGANYKPYGG TFLNFVSYAA GAVRLAALSG NPVIWVATHD SIGLGEDGPT 480
  481 HQPIETLAHL RAIPNMHVWR PADGNETSAA YYSAIKSGRT PSVVALSRQN LPQLEHSSFE 540
  541 KALKGGYVIH DVENPDIILV STGSEVSISI DAAKKLYDTK KIKARVVSLP DFYTFDRQSE 600
  601 EYRFSVLPDG VPIMSFEVLA TSSWGKYAHQ SFGLDEFGRS GKGPEIYKLF DFTADGVASR 660
  661 AEKTINYYKG KQLLSPMGRA F

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) TKL1 TKL2
    View Details Krogan NJ, et al. (2006) ACS2 TKL1 TKL2
    View Details Gavin AC, et al. (2002) TKL1 TKL2
    View Details Qiu et al. (2008) AST2 TKL2 TPS1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #25 Asynchronous Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #19b Asynchronous Prep1-new search criteria Keck JM, et al. (2011)
    View Run MLP2 #19a Asynchronous Prep1-new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data TKL2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..128]
    [148..310]    		 PSI-BLAST 11000.0 Transketolase (TK), Pyr module; Transketolase (TK), PP module; Transketolase (TK), C-domain
    2 View Details [129..147]
    [311..340]    		 PSI-BLAST 11000.0 Transketolase (TK), Pyr module; Transketolase (TK), PP module; Transketolase (TK), C-domain
    3 View Details [341..366]
    [465..681]    		 PSI-BLAST 11000.0 Transketolase (TK), Pyr module; Transketolase (TK), PP module; Transketolase (TK), C-domain
    4 View Details [367..464] PSI-BLAST 11000.0 Transketolase (TK), Pyr module; Transketolase (TK), PP module; Transketolase (TK), C-domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • transketolase activity
    		•  7.85070252105246 bayes_pls_golite062009
  • transferase activity, transferring aldehyde or ketonic groups
    		•  4.74831149446647 bayes_pls_golite062009
  • oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
    		•  2.93250853993668 bayes_pls_golite062009
  • pyruvate dehydrogenase activity
    		•  2.81702263447118 bayes_pls_golite062009
  • pyruvate dehydrogenase (acetyl-transferring) activity
    		•  2.6977837113283 bayes_pls_golite062009
  • catalytic activity
    		•  2.25537597692103 bayes_pls_golite062009
  • binding
    		•  0.980920092099572 bayes_pls_golite062009
  • transferase activity
    		•  0.413024577830522 bayes_pls_golite062009
  • acetolactate synthase activity
    		•  0.159086519260601 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.71

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle