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Protein Overview: TKL1

General Information:
Name(s) found: TKL1 / YPR074C [SGD]
Description(s) found:
Found 43 descriptions.
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  • Transketolase, similar to Tkl2p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 680 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [ISSLink to PubMed article]
    Biological Process: pentose-phosphate shunt [IMPLink to PubMed article][TASLink to PubMed article]
    Molecular Function: transketolase activity [IMPLink to PubMed article][ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTQFTDIDKL AVSTIRILAV DTVSKANSGH PGAPLGMAPA AHVLWSQMRM NPTNPDWINR  60
   61 DRFVLSNGHA VALLYSMLHL TGYDLSIEDL KQFRQLGSRT PGHPEFELPG VEVTTGPLGQ 120
  121 GISNAVGMAM AQANLAATYN KPGFTLSDNY TYVFLGDGCL QEGISSEASS LAGHLKLGNL 180
  181 IAIYDDNKIT IDGATSISFD EDVAKRYEAY GWEVLYVENG NEDLAGIAKA IAQAKLSKDK 240
  241 PTLIKMTTTI GYGSLHAGSH SVHGAPLKAD DVKQLKSKFG FNPDKSFVVP QEVYDHYQKT 300
  301 ILKPGVEANN KWNKLFSEYQ KKFPELGAEL ARRLSGQLPA NWESKLPTYT AKDSAVATRK 360
  361 LSETVLEDVY NQLPELIGGS ADLTPSNLTR WKEALDFQPP SSGSGNYSGR YIRYGIREHA 420
  421 MGAIMNGISA FGANYKPYGG TFLNFVSYAA GAVRLSALSG HPVIWVATHD SIGVGEDGPT 480
  481 HQPIETLAHF RSLPNIQVWR PADGNEVSAA YKNSLESKHT PSIIALSRQN LPQLEGSSIE 540
  541 SASKGGYVLQ DVANPDIILV ATGSEVSLSV EAAKTLAAKN IKARVVSLPD FFTFDKQPLE 600
  601 YRLSVLPDNV PIMSVEVLAT TCWGKYAHQS FGIDRFGASG KAPEVFKFFG FTPEGVAERA 660
  661 QKTIAFYKGD KLISPLKKAF

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ADH1 EFT2, EFT1 HSC82 TKL1 TKL2
    View Details Krogan NJ, et al. (2006) ACS2 TKL1 TKL2
    View Details Gavin AC, et al. (2006) MET6 TKL1 TSA1
    View Details Gavin AC, et al. (2002) TKL1 TKL2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data TKL1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..128]
    [148..310]    		 PSI-BLAST 11000.0 Transketolase (TK), Pyr module; Transketolase (TK), PP module; Transketolase (TK), C-domain
    2 View Details [129..147]
    [311..340]    		 PSI-BLAST 11000.0 Transketolase (TK), Pyr module; Transketolase (TK), PP module; Transketolase (TK), C-domain
    3 View Details [341..366]
    [465..680]    		 PSI-BLAST 11000.0 Transketolase (TK), Pyr module; Transketolase (TK), PP module; Transketolase (TK), C-domain
    4 View Details [367..464] PSI-BLAST 11000.0 Transketolase (TK), Pyr module; Transketolase (TK), PP module; Transketolase (TK), C-domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • transketolase activity
    		•  6.17852985073866 bayes_pls_golite062009
  • oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
    		•  2.93250853993668 bayes_pls_golite062009
  • pyruvate dehydrogenase activity
    		•  2.81702263447118 bayes_pls_golite062009
  • pyruvate dehydrogenase (acetyl-transferring) activity
    		•  2.6977837113283 bayes_pls_golite062009
  • transferase activity, transferring aldehyde or ketonic groups
    		•  2.21959520697519 bayes_pls_golite062009
  • binding
    		•  0.980920092099572 bayes_pls_golite062009
  • catalytic activity
    		•  0.545407789079971 bayes_pls_golite062009
  • thiamin pyrophosphate binding
    		•  0.297191393111081 bayes_pls_golite062009
  • acetolactate synthase activity
    		•  0.173822256668691 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.82

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle