Protein Overview: RPP1B

General Information:
Name(s) found:	RPP1B / YDL130W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|R8BY2B acidic ribosomal protein P1.e.B, cytosolic - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6320073\|ref\|NP_010153.1\| Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk com [NCBI NR] gi\|172400\|gb\|AAA34973.1\| ribosomal protein L44' [NCBI NR] gi\|171815\|gb\|AAA34734.1\| L12eIIB protein [NCBI NR] gi\|1431197\|emb\|CAA98698.1\| RPP1B [Saccharomyces cerevisiae] [NCBI NR] gi\|133069\|sp\|P10622.3\|RLA3_YEAST RecName: Full=60S acidic ribosomal protein P1-beta; Short=P1B; AltName: Full=YP1beta; AltName: Full=Ax; AltName: Full=L44'; AltName: Full=L12EIIB [NCBI NR] 60S acidic ribosomal protein P1-beta OS=Saccharomyces cerevisiae GN=RPP1B PE=1 SV=3 [Swiss-Prot] RPP1B 60S large subunit acidic ribosomal protein L44prime [mips] Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component [SGD] RPP1B, Chr IV from 229906-230019,230321-230527 [yeast_orfs2] Chr IV from 229906-230019,230321-230527 [yeastorf3.fasta] (P10622) 60S acidic ribosomal protein P1-beta (L44') (L12EIIB) [4932.FASTACCERV.txt] RPP1B SGDID:S0002288, Chr IV from 229906-230019,230321-230527, Verified ORF [yeastflysequest.fasta] Ribosomal protein P1 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 [nrdb_11152004_Chlamydomonas] RPP1B SGDID:S000002288, Chr IV from 229906-230019,230321-230527, Verified ORF, "Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by p [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ribosomal protein L44' [nr-271106-contam.fasta] Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component; Rpp1bp [Saccharomyce [nr-271106-contam.fasta] 60S acidic ribosomal protein P1-beta (L44') (L12EIIB) [nr-271106-contam.fasta] RPP1B [Saccharomyces cerevisiae] [nr-271106-contam.fasta] L12eIIB protein [nr-271106-contam.fasta] RPP1B SGDID:S000002288, Chr IV from 229906-230019,230321-230527, Verified ORF, "Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component" [yeast-bovrabprots.fasta] [chemostatdb.fasta] 60S acidic ribosomal protein P1-beta OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPP1B PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RPP1B SGDID:S000002288, Chr IV from 229906-230019,230321-230527, Genome Release 64-1-1, Verified ORF, "Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component" [yeast-030211-contam.fasta] RPP1B SGDID:S000002288, Chr IV from 229906-230019,230321-230527, Genome Release 64-2-1, Verified ORF, "Ribosomal protein P1 beta; component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation is regulated by phosphorylation and interaction with the P2 stalk component" [20141208_orf_trans.fasta] TR7318\|c0_g1_i1\|g.5342 ORF TR7318\|c0_g1_i1\|g.5342 TR7318\|c0_g1_i1\|m.5342 type:complete len:107 (+) TR7318\|c0_g1_i1:114-434(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YDL130W SGDID:S000002288, Chr IV from 229906-230019,230321-230527, Genome Release 64-1-1, Verified ORF, "Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_493\|g.729 ORF asmbl_493\|g.729 asmbl_493\|m.729 type:complete len:107 (+) asmbl_493:120-440(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_495\|g.653 ORF ID=asmbl_495\|g.653 ID=asmbl_495\|m.653 type:complete len:107 (+) ID=asmbl_495:120-440(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	106 amino acids
Gene Ontology:
Cellular Component:	ribonucleoprotein complex [IEA] cytoplasm [IEA] cytosolic large ribosomal subunit [TAS] ribosome [IEA] intracellular [IEA]
Biological Process:	translational elongation [ISS ] translation [TAS]
Molecular Function:	structural constituent of ribosome [TAS]

Sequence:
Sequence: [PDR BLAST] [ProtParam]	1 11 21 31 41 51 \| \| \| \| \| \| 1 MSDSIISFAA FILADAGLEI TSDNLLTITK AAGANVDNVW ADVYAKALEG KDLKEILSGF 60 61 HNAGPVAGAG AASGAAAAGG DAAAEEEKEE EAAEESDDDM GFGLFD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL14B RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL20B RPL21A RPL21B RPL22A RPL22B RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27A RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL34B RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL4B RPL5 RPL6A RPL6B RPL7A RPL7B RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B
View Details	Krogan NJ, et al. (2006)		ADE3 MIS1 RPP1B TMA108
View Details	Qiu et al. (2008)		AAC3 ASC1 BUD20 EFT2, EFT1 FYV12 HMS2 MNL1 NAT3 RIA1 RKI1 RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL14B RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL20B RPL21A RPL21B RPL22A RPL22B RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27A RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL34B RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL4B RPL5 RPL6A RPL6B RPL7A RPL7B RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B RPS0A RPS0B RPS10A RPS10B RPS11A, RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16B, RPS16A RPS17A RPS17B RPS18B, RPS18A RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B RPS23B, RPS23A RPS24A, RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30B, RPS30A RPS31 RPS4B, RPS4A RPS5 RPS6A, RPS6B RPS7A RPS7B RPS8B, RPS8A RPS9A RPS9B SFT1 STM1 TEF1, TEF2 TSR1 YDL133W YML6 YOR292C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample zds1 from august 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#34 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	EUG1	RPP1B	1	Unpublished Fields Lab Data
View Screen	RPP0	RPP1B	1	Unpublished Fields Lab Data
View Screen	APC5	RPP1B	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	RPP1B	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..106]	Pfam	39.677781	No description for PF00428.10 was found. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2