Protein Overview: THI3

General Information:
Name(s) found:	THI3 / YDL080C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320123\|ref\|NP_010203.1\| Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis; Thi3p [Saccharomyces cerevisia [NCBI NR] gi\|2501334\|sp\|Q07471.1\|THI3_YEAST RecName: Full=Thiamine metabolism regulatory protein THI3; AltName: Full=Keto isocaproate decarboxylase KID1 [NCBI NR] pir\|\|S67616 probable membrane protein YDL080c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1431100\|emb\|CAA98646.1\| THI3 [Saccharomyces cerevisiae] [NCBI NR] Thiamine metabolism regulatory protein THI3 OS=Saccharomyces cerevisiae GN=THI3 PE=1 SV=1 [Swiss-Prot] THI3 Positive regulation factor of thiamin metabolism [mips] Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis [SGD] THI3, Chr IV from 310641-312470, reverse complement [yeast_orfs2] Chr IV from 310641-312470, reverse complement [yeastorf3.fasta] Chr IV from 312470-310641, reverse complement [yeastorf5.fasta] (Q07471) Thiamine metabolism regulatory protein THI3 [4932.FASTACCERV.txt] THI3 SGDID:S0002238, Chr IV from 312471-310642, reverse complement, Verified ORF [yeastflysequest.fasta] Probable decarboxylase, required for expression of enzymes involved in thiamine bioysynthesis; may have a role in catabolism of amino acids to long-chain and complex alcohols [Saccharomyces cerevisiae]gi\|1431100\|emb\|CAA98646.1\| THI3 [Saccharomyces cerevi [nrdb_11152004_Chlamydomonas] (Q07471) Thiamine metabolism regulatory protein THI3 (EC 4.1.1.-) (Keto isocaproate decarboxylase KID1) [cerv4932.FASTAC] THI3 SGDID:S000002238, Chr IV from 312471-310642, reverse complement, Verified ORF, "Probable decarboxylase, required for expression of enzymes involved in thiamine biosynthesis; may have a role in catabolism of amino acids to long-chain and complex alcoh [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] THI3 SGDID:S000002238, Chr IV from 312471-310642, reverse complement, Verified ORF, "Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Thiamine metabolism regulatory protein THI3 (Keto isocaproate decarboxylase KID1) [nr-271106-contam.fasta] THI3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Probable decarboxylase, required for expression of enzymes involved in thiamine biosynthesis; may have a role in catabolism of amino acids to long-chain and complex alcohols; Thi3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] THI3 SGDID:S000002238, Chr IV from 312471-310642, reverse complement, Verified ORF, "Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis" [yeast-bovrabprots.fasta] THI3 SGDID:S000002238, Chr IV from 312471-310642, reverse complement, Verified ORF, "Probable decarboxylase, required for expression of enzymes involved in thiamine biosynthesis; may have a role in catabolism of amino acids to long-chain and complex alcohols" [yeast-contam_custom.fasta] [chemostatdb.fasta] Thiamine metabolism regulatory protein THI3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=THI3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] THI3 SGDID:S000002238, Chr IV from 312471-310642, Genome Release 64-1-1, reverse complement, Verified ORF, "Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis" [yeast-030211-contam.fasta] THI3 SGDID:S000002238, Chr IV from 312471-310642, Genome Release 64-2-1, reverse complement, Verified ORF, "Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected" [20141208_orf_trans.fasta] YDL080C SGDID:S000002238, Chr IV from 312471-310642, Genome Release 64-1-1, reverse complement, Verified ORF, "Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	609 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA ] cytosol [IDA]
Biological Process:	positive regulation of transcription from RNA polymerase II promoter [IMP] thiamin biosynthetic process [IMP][IPI][TAS]
Molecular Function:	transcription activator activity [IMP] carboxy-lyase activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNSSYTQRYA LPKCIAISDY LFHRLNQLNI HTIFGLSGEF SMPLLDKLYN IPNLRWAGNS  60
   61 NELNAAYAAD GYSRLKGLGC LITTFGVGEL SAINGVAGSY AEHVGILHIV GMPPTSAQTK 120
  121 QLLLHHTLGN GDFTVFHRIA SDVACYTTLI IDSELCADEV DKCIKKAWIE QRPVYMGMPV 180
  181 NQVNLPIESA RLNTPLDLQL HKNDPDVEKE VISRILSFIY KSQNPAIIVD ACTSRQNLIE 240
  241 ETKELCNRLK FPVFVTPMGK GTVNETDPQF GGVFTGSISA PEVREVVDFA DFIIVIGCML 300
  301 SEFSTSTFHF QYKTKNCALL YSTSVKLKNA TYPDLSIKLL LQKILANLDE SKLSYQPSEQ 360
  361 PSMMVPRPYP AGNVLLRQEW VWNEISHWFQ PGDIIITETG ASAFGVNQTR FPVNTLGISQ 420
  421 ALWGSVGYTM GACLGAEFAV QEINKDKFPA TKHRVILFMG DGAFQLTVQE LSTIVKWGLT 480
  481 PYIFVMNNQG YSVDRFLHHR SDASYYDIQP WNYLGLLRVF GCTNYETKKI ITVGEFRSMI 540
  541 SDPNFATNDK IRMIEIMLPP RDVPQALLDR WVVEKEQSKQ VQEENENSSA VNTPTPEFQP 600
  601 LLKKNQVGY

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2002)		CCT2 FYV8 THI3
View Details	Ho Y, et al. (2002)		BFR2 BIR1 BNA5 CDC7 ECM10 JIP3 NUT1 PDC5 PDC6 PST2 RPC19 SAR1 SEC27 STI1 THI3 TPS1 UBI4 VAC14

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#16 Mitotic-New search criteria	Keck JM, et al. (2011)
View Run	None Entered	#18 Mitotic Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC16	THI3	1	Unpublished Fields Lab Data
View Screen	RGD2	THI3	1	Unpublished Fields Lab Data
View Screen	TOS8	THI3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	THI3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..199]	PSI-BLAST	1921.0	Pyruvate decarboxylase
2	View Details	[200..364]	PSI-BLAST	1921.0	Pyruvate decarboxylase
3	View Details	[365..609]	PSI-BLAST	1921.0	Pyruvate decarboxylase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
acetolactate synthase activity	12.903449876837	bayes_pls_golite062009
transferase activity, transferring aldehyde or ketonic groups	12.3239025230705	bayes_pls_golite062009
pyruvate dehydrogenase activity	5.28857071459273	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor	5.21233670052586	bayes_pls_golite062009
pyruvate dehydrogenase (acetyl-transferring) activity	5.08663245333808	bayes_pls_golite062009
NAD or NADH binding	3.93430700665136	bayes_pls_golite062009
transcription regulator activity	2.9155913558118	bayes_pls_golite062009
DNA binding	2.43141001866996	bayes_pls_golite062009
nucleic acid binding	2.41433477472968	bayes_pls_golite062009
catalytic activity	2.25537597692103	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors	2.15444163802756	bayes_pls_golite062009
transketolase activity	2.13705987575498	bayes_pls_golite062009
NAD-dependent protein deacetylase activity	2.09493047697397	bayes_pls_golite062009
NAD-dependent histone deacetylase activity	2.09493047697397	bayes_pls_golite062009
histone deacetylase activity	2.07818242335598	bayes_pls_golite062009
protein deacetylase activity	2.07076853700185	bayes_pls_golite062009
deoxyhypusine synthase activity	2.04735838056767	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	1.98546381612935	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	1.82969293063325	bayes_pls_golite062009
hydrolase activity	1.43067365161346	bayes_pls_golite062009
transcription factor activity	1.30143289828088	bayes_pls_golite062009
deacetylase activity	1.21596867135219	bayes_pls_golite062009
binding	1.17940168894417	bayes_pls_golite062009
transcription factor binding	1.14766678956419	bayes_pls_golite062009
phenylpyruvate decarboxylase activity	1.11553401986553	bayes_pls_golite062009
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity	1.07090849835369	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	1.0611726850452	bayes_pls_golite062009
protein binding	0.992849906276347	bayes_pls_golite062009
cation transmembrane transporter activity	0.78591260444574	bayes_pls_golite062009
alpha-ketoacid dehydrogenase activity	0.750929351082237	bayes_pls_golite062009
transcription repressor activity	0.614654207326026	bayes_pls_golite062009
transcription cofactor activity	0.54005617175343	bayes_pls_golite062009
ion transmembrane transporter activity	0.51426028502998	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	0.492590326775569	bayes_pls_golite062009
transferase activity	0.413024577830522	bayes_pls_golite062009
transcription corepressor activity	0.40721151987565	bayes_pls_golite062009
pyruvate decarboxylase activity	0.198062360371269	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	0.114062221123972	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.89

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2