Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #31 Asynchronous Prep (Protease cleavage) |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
RPP1B | Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of tra... | 5 | 18 | 64.2% | 0.0492 | 10668 |
[ |
SEM1 | Component of the lid subcomplex of the regulatory subunit of the 26S proteasome; involved in mRNA ex... | 3 | 11 | 62.9% | 0.0358 | 10386 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 3 | 8 | 55.1% | 0.0158 | 16135 |
[ |
RPP1A | Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation facto... | 3 | 16 | 48.1% | 0.0438 | 10908 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 3 | 22 | 43.6% | 0.058 | 11050 |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 4 | 14 | 43.4% | 0.0383 | 10746 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 2 | 3 | 31.9% | 0.0126 | 7729 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 3 | 6 | 30.4% | 0.0108 | 18751 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 9 | 27 | 26.7% | 0.0216 | 42271 |
[ |
PSA1 | GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... | 6 | 11 | 22.7% | 0.00884 | 39566 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 31 | 75 | 22.2% | 0.013 | 195140 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 2 | 65 | 19.1% | 0.0461 | 44671 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 9 | 26 | 17.6% | 0.013 | 67400 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 6 | 33 | 17.0% | 0.0191 | 54545 |
[ |
SAM1 | S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... | 3 | 22 | 15.7% | 0.0167 | 41818 |
[ |
RPN8 | Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 prot... | 2 | 10 | 13.9% | 0.00858 | 38313 |
[ |
SAM2 | S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... | 2 | 12 | 12.8% | 0.00906 | 42256 |
[ |
RAD51 | Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in t... | 2 | 2 | 12.5% | 0.00145 | 42963 |
[ |
RPT5 | One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation ... | 2 | 3 | 11.1% | 0.002 | 48256 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 10 | 28 | 11.0% | 0.0086 | 111782 |
[ |
YBR287W | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR... | 2 | 2 | 11.0% | 0.00136 | 47506 |
[ |
CPR6 | Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide ... | 2 | 15 | 10.2% | 0.0117 | 42072 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 3 | 5 | 9.9% | 0.00253 | 66087 |
[ |
SBP1 | Putative RNA binding protein; involved in translational repression and found in cytoplasmic P bodies... | 2 | 53 | 9.9% | 0.0523 | 32990 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 6 | 21 | 9.1% | 0.00893 | 74468 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 2 | 2 | 8.7% | 0.00127 | 50033 |
[ |
YOR051C | Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model s... | 2 | 6 | 8.7% | 0.00422 | 47352 |
[ |
URA7 | Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrog... | 2 | 17 | 8.6% | 0.00851 | 64710 |
[ |
CCT5 | Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... | 2 | 3 | 8.4% | 0.00155 | 61914 |
[ |
NFU1 | Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required fo... | 2 | 4 | 8.2% | 0.00453 | 29174 |
[ |
DEF1 | RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... | 2 | 19 | 7.9% | 0.00747 | 83973 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 3 | 3 | 7.9% | 0.00123 | 80900 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 4 | 8 | 7.8% | 0.00276 | 93289 |
[ |
RPN7 | Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae reg... | 2 | 24 | 7.7% | 0.0162 | 48959 |
[ |
PGK1 | 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... | 2 | 2 | 7.7% | 0.00139 | 44738 |
[ |
PDI1 | Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... | 4 | 16 | 7.5% | 0.00889 | 58227 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 2 | 4 | 7.5% | 0.00364 | 37354 |
[ |
CDC48 | ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p a... | 3 | 24 | 6.9% | 0.00834 | 91996 |
[ |
ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... | 2 | 6 | 6.9% | 0.00464 | 41690 |
[ |
GRS1 | Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bea... | 3 | 5 | 6.7% | 0.00217 | 75411 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 3 | 9 | 6.3% | 0.0103 | 29280 |
[ |
ARB1 | ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has sim... | 2 | 2 | 6.2% | 9.51E-4 | 68377 |
[ |
HSP42 | Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress... | 2 | 4 | 6.1% | 0.00309 | 42817 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 2 | 5 | 6.0% | 0.00202 | 80047 |
[ |
CPA2 | Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulli... | 5 | 9 | 5.5% | 0.00233 | 123915 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 2 | 2 | 5.4% | 6.82E-4 | 94104 |
[ |
NPL3 | RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... | 2 | 3 | 5.3% | 0.0021 | 45407 |
[ |
ADE4 | Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes fir... | 2 | 4 | 4.9% | 0.00227 | 56719 |
[ |
CHC1 | Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport ... | 3 | 4 | 4.7% | 7.02E-4 | 187233 |
[ |
MAK21 | Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; i... | 2 | 4 | 4.0% | 0.00113 | 116676 |
[ |
SSC1 | Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... | 2 | 7 | 4.0% | 0.0031 | 70628 |
[ |
YHR020W | Protein of unknown function that may interact with ribosomes, based on co-purification experiments; ... | 2 | 6 | 3.9% | 0.00253 | 77386 |
[ |
YEF3 | Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... | 2 | 25 | 3.3% | 0.00694 | 115945 |
[ |
FAS2 | Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty ... | 3 | 32 | 3.2% | 0.00492 | 206945 |
[ |
SUP35 | Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... | 2 | 7 | 2.6% | 0.00296 | 76551 |
[ |
FAS1 | Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty a... | 2 | 4 | 2.0% | 5.66E-4 | 228689 |
[ |
URA2 | Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... | 3 | 6 | 1.9% | 7.86E-4 | 245123 |
[ |
ACC1 | Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... | 3 | 4 | 1.4% | 5.19E-4 | 250351 |