DTASelect v2.0.16 /data/1/catclw/Projects/Shelly/step_1/re_dta /garibaldi/people-b/applications/yates/dbase/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta SEQUEST 3.0 in SQT format. -p 1 --fp 0.1 --modstat --sp --noxc --nodcn --extra -e con true Use criteria 0.0 Minimum peptide confidence 0.1 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling con Exclude protein names matching 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YDL130W 5 18 64.2% 106 10668 4.0 U RPP1B SGDID:S000002288, Chr IV from 229906-230019,230321-230527, Verified ORF, "Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component" * 22_g_01_itms.09273.09273.2 3.6216 0.4137 100.0 1664.1322 1663.808 8460.7 1 6.292 56.7 2 K.AAGANVDNVWADVYAK.A * 22_g_01_itms.15060.15060.3 6.4974 0.5196 100.0 5092.224 5091.1274 5870.5 1 8.524 20.1 9 K.EILSGFHNAGPVAGAGAASGAAAAGGDAAAEEEKEEEAAEES*DDDMGFGLFD.- * 22_g_01_itms.12324.12324.3 4.4982 0.0648 100.0 3473.1843 3471.322 9486.9 1 5.778 27.3 1 A.SGAAAAGGDAAAEEEKEEEAAEES*DDDMGFGLFD.- * 22_g_01_itms.12305.12305.3 4.8778 0.3402 100.0 3257.0645 3256.2312 9131.7 1 7.053 42.5 4 A.AAAGGDAAAEEEKEEEAAEES*DDDMGFGLFD.- * 22_g_01_itms.12343.12343.2 4.1403 0.3926 100.0 3257.3323 3256.2312 6488.0 1 6.763 35.0 2 A.AAAGGDAAAEEEKEEEAAEES*DDDMGFGLFD.- YDR363W-A 3 11 62.9% 89 10386 4.3 U SEM1 SGDID:S000007235, Chr IV from 1202118-1202387, Verified ORF, "Component of the lid subcomplex of the regulatory subunit of the 26S proteasome; ortholog of human DSS1" * 22_g_01_itms.13577.13577.2 5.0436 0.4469 100.0 2700.7122 2700.184 8500.9 1 8.333 47.7 4 K.SLEEDDEFEDFPIDTWANGETIK.S * 22_g_01_itms.13661.13661.3 5.5318 0.3577 100.0 2701.0444 2700.184 10525.2 1 7.482 33.0 6 K.SLEEDDEFEDFPIDTWANGETIK.S * 22_g_01_itms.19824.19824.3 3.7327 0.2302 98.1 3915.9543 3910.7532 10086.0 3 4.309 21.1 1 K.SNAVTQTNIWEENWDDVEVDDDFTNELKAELDR.Y YBR109C 3 8 55.1% 147 16135 4.3 U CMD1 SGDID:S000000313, Chr II from 458356-457913, reverse complement, Verified ORF, "Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin" * 22_g_01_itms.23873.23873.3 4.2227 0.1669 99.0 4110.1143 4106.9326 8614.6 2 4.666 20.1 1 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q * 22_g_01_itms.10553.10553.2 4.2442 0.3819 100.0 1509.8121 1509.7073 10958.0 1 7.136 79.2 4 K.SNDSEQELLEAFK.V * 22_g_01_itms.26644.26644.3 4.152 0.3101 100.0 3367.6143 3366.601 7891.4 3 5.409 21.7 3 T.SIGEKLTDAEVDDMLREVSDGSGEINIQQFA.A YDL081C 3 16 48.1% 106 10908 3.9 U RPP1A SGDID:S000002239, Chr IV from 310122-309802, reverse complement, Verified ORF, "Ribosomal protein P1 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component" * 22_g_01_itms.16146.16146.3 4.1985 0.277 100.0 5111.1846 5109.1997 6942.2 1 5.602 16.5 2 K.DLLVNFSAGAAAPAGVAGGVAGGEAGEAEAEKEEEEAKEES*DDDMGFGLFD.- * 22_g_01_itms.12953.12953.3 6.7218 0.4251 100.0 3813.2043 3811.533 7339.5 1 7.83 26.4 9 A.GVAGGVAGGEAGEAEAEKEEEEAKEES*DDDMGFGLFD.- * 22_g_01_itms.12858.12858.3 5.5152 0.3892 100.0 3656.3643 3655.443 8566.7 1 6.874 27.9 5 V.AGGVAGGEAGEAEAEKEEEEAKEES*DDDMGFGLFD.- YDR382W 3 22 43.6% 110 11050 4.1 U RPP2B SGDID:S000002790, Chr IV from 1239482-1239814, Verified ORF, "Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm" * 22_g_01_itms.12434.12434.3 4.9382 0.3209 100.0 4693.5244 4686.947 6745.9 1 5.422 19.1 5 K.FATVPTGGASSAAAGAAGAAAGGDAAEEEKEEEAKEES*DDDMGFGLFD.- * 22_g_01_itms.11879.11879.3 3.7805 0.2795 97.7 3640.7644 3640.4434 10036.4 1 4.926 18.6 2 A.AAGAAGAAAGGDAAEEEKEEEAKEES*DDDMGFGLFD.- * 22_g_01_itms.13068.13068.3 4.0503 0.2568 100.0 3177.3843 3171.2148 6486.2 1 5.897 38.4 15 A.AAGGDAAEEEKEEEAKEES*DDDMGFGLFD.- YOL039W 4 14 43.4% 106 10746 4.0 U RPP2A SGDID:S000005399, Chr XV from 254295-254615, Verified ORF, "Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm" * 22_g_01_itms.13023.13023.3 8.0701 0.5539 100.0 4454.5444 4447.856 8079.8 1 9.512 30.6 4 K.LAAVPAAGPASAGGAAAASGDAAAEEEKEEEAAEES*DDDMGFGLFD.- * 22_g_01_itms.12281.12281.3 4.2879 0.3623 100.0 3289.4944 3286.242 9084.8 1 5.967 25.0 1 A.AAASGDAAAEEEKEEEAAEES*DDDMGFGLFD.- * 22_g_01_itms.12757.12757.2 4.3677 0.4254 100.0 3073.4321 3073.1306 6143.9 1 7.349 38.9 4 A.SGDAAAEEEKEEEAAEES*DDDMGFGLFD.- * 22_g_01_itms.12702.12702.3 5.0175 0.4188 100.0 3077.2744 3073.1306 6163.4 1 7.185 34.3 5 A.SGDAAAEEEKEEEAAEES*DDDMGFGLFD.- Reverse_YPR036W-A 1 1 40.3% 67 7055 5.9 U YPR036W-A SGDID:S000028425, Chr XVI from 645947-646150, Uncharacterized ORF * 22_g_01_itms.22372.22372.2 2.6616 0.284 95.6 2830.7122 2830.4224 10791.1 21 4.546 26.9 1 N.ADSNKRSSPQSVPSLSPIVFPQSVDST.L YIL002W-A 2 3 31.9% 69 7729 4.7 U YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry" * 22_g_01_itms.00239.00239.2 3.049 0.4073 100.0 1191.0122 1190.5541 3260.9 1 6.427 77.3 1 R.DTPEDVSTAGAK.D * 22_g_01_itms.16829.16829.2 3.4302 0.223 98.4 1156.2922 1155.6989 5435.2 2 4.848 83.3 2 K.DILDVLNLLK.G YOR257W 3 6 30.4% 161 18751 4.6 U CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" * 22_g_01_itms.10425.10425.2 2.8888 0.172 92.0 2593.0322 2592.268 8910.8 1 4.356 31.8 1 R.SSLQSGPLNSELLEEQKQEIYEA.F * 22_g_01_itms.11359.11359.2 4.6419 0.41 100.0 1668.4521 1666.7811 6383.1 1 7.434 80.8 2 R.EILDLIDEYDSEGR.H * 22_g_01_itms.06696.06696.2 3.1462 0.3936 100.0 1406.2522 1404.6858 7552.9 12 6.295 63.6 3 K.ELGETLTDEELR.A Reverse_YOR020C 1 1 29.2% 106 11372 9.0 U HSP10 SGDID:S000005546, Chr XV from 370843-370523, reverse complement, Verified ORF, "Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES" * 22_g_01_itms.15188.15188.3 2.8649 0.1703 90.2 3238.5244 3233.717 3960.8 443 3.802 20.0 1 Q.PVVKNGNADTFGPGVAVVEAQNLKEVNKEPL.Y YKL042W 9 27 26.7% 363 42271 7.9 U SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane" * 22_g_01_itms.09764.09764.2 3.1006 0.3262 94.6 2607.392 2607.0903 7196.8 3 5.361 36.8 1 R.LYDDYYNIPYQYSNPT*PMNR.D * 22_g_01_itms.10109.10109.2 3.0547 0.3982 100.0 1297.1921 1296.6986 9237.0 1 6.804 80.0 3 R.TLSVLTNYVMR.S * 22_g_01_itms.12015.12015.2 2.8979 0.3483 97.1 1376.7722 1376.6649 6208.7 1 5.491 80.0 1 R.TLS*VLTNYVMR.S * 22_g_01_itms.13505.13505.3 5.16 0.2287 97.7 2848.7043 2844.3352 7135.1 1 4.945 36.5 4 R.SEDGNNDRMS*PLPSPLNTILPINNR.L * 22_g_01_itms.13451.13451.3 5.0754 0.2547 92.5 2927.5745 2924.3015 7708.9 1 4.76 31.2 8 R.SEDGNNDRMS*PLPS*PLNTILPINNR.L * 22_g_01_itms.15361.15361.3 5.209 0.2395 98.2 2615.1243 2614.1895 9120.0 1 5.272 34.5 1 R.KLS*LNNQLQELQSMMDGDDNIK.L * 22_g_01_itms.05137.05137.2 4.0908 0.3265 100.0 1958.5521 1956.8414 7529.2 1 5.988 53.1 1 R.VKPENNMSETFAT*PTPN.N * 22_g_01_itms.09851.09851.3 3.9872 0.1876 92.3 2228.3943 2226.9854 6346.5 12 4.769 36.1 1 R.VKPENNMSETFATPT*PNNR.- * 22_g_01_itms.08207.08207.3 4.3433 0.2587 98.3 2228.6343 2226.9854 5546.0 5 5.226 33.3 7 R.VKPENNMSETFAT*PTPNNR.- YLL024C 13 35 25.7% 639 69470 5.1 U SSA2 SGDID:S000003947, Chr XII from 97484-95565, reverse complement, Verified ORF, "ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall" * 22_g_01_itms.07743.07743.2 2.0348 0.1965 92.0 1457.7522 1457.6913 4131.4 36 4.368 58.3 1 R.TTPSFVGFTDTER.L 22_g_01_itms.14927.14927.3 3.2954 0.3067 99.7 1677.0844 1675.7312 4924.6 1 4.883 41.7 2 K.ATAGDTHLGGEDFDNR.L 22_g_01_itms.14049.14049.3 3.5944 0.3016 100.0 2578.3145 2577.2683 9710.3 3 5.176 28.0 2 R.SINPDEAVAYGAAVQAAILTGDESSK.T 22_g_01_itms.14083.14083.2 5.3564 0.5322 100.0 2581.8323 2577.2683 10901.7 1 8.995 44.0 3 R.SINPDEAVAYGAAVQAAILTGDESSK.T 22_g_01_itms.14431.14431.2 3.8239 0.2324 100.0 2586.4922 2585.2524 7916.0 1 6.632 36.4 2 K.SEVFSTYADNQPGVLIQVFEGER.A 22_g_01_itms.09411.09411.2 2.6828 0.1985 99.6 1265.7522 1265.6742 8448.0 42 5.213 65.0 2 K.NQLESIAYSLK.N * 22_g_01_itms.45461.45461.3 3.1351 0.1858 96.3 2134.8245 2133.0676 4665.9 95 4.18 31.6 2 K.NTISEAGDKLEQADKDAVTK.K * 22_g_01_itms.17415.17415.3 4.8202 0.0724 100.0 2364.9243 2363.144 9130.3 1 5.565 43.4 7 K.EEFDDQLKELQEVANPIMSK.L * 22_g_01_itms.09587.09587.3 4.085 0.2249 100.0 3390.7444 3390.613 8164.8 1 5.153 23.6 5 S.KLYQAGGAPEGAAPGGFPGGAPPAPEAEGPTVEEVD.- * 22_g_01_itms.06929.06929.3 3.4894 0.2284 99.0 2437.3145 2436.1472 6120.2 19 4.653 26.9 2 K.LYQAGGAPEGAAPGGFPGGAPPAPEAE.G * 22_g_01_itms.08337.08337.2 2.7492 0.0529 100.0 3264.5322 3262.518 4054.1 1 6.028 33.8 1 K.LYQAGGAPEGAAPGGFPGGAPPAPEAEGPTVEEVD.- * 22_g_01_itms.08339.08339.3 4.2236 0.0821 100.0 3267.6543 3262.518 6132.1 17 5.335 22.1 2 K.LYQAGGAPEGAAPGGFPGGAPPAPEAEGPTVEEVD.- 22_g_01_itms.08339.08339.2 3.5598 0.3224 100.0 2178.7722 2177.004 6132.1 27 6.138 34.1 4 A.PGGFPGGAPPAPEAEGPTVEEVD.- YGR271C-A 1 2 25.4% 63 7099 4.3 U YGR271C-A SGDID:S000007608, Chr VII from 1037997-1037806, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus" * 22_g_01_itms.09590.09590.2 3.401 0.2588 100.0 1914.3322 1913.8292 9441.4 1 5.62 56.7 2 K.ELDQVVGEDEKDDFFE.- YGL258W-A 1 1 23.4% 77 8690 8.8 U YGL258W-A SGDID:S000007607, Chr VII from 9162-9395, Uncharacterized ORF, "Hypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36." * 22_g_01_itms.00067.00067.2 1.5681 0.2836 95.7 1968.0521 1968.0231 5088.3 62 4.611 23.5 1 N.VHFGSILFGAVDKSKYAE.E YDL055C 6 11 22.7% 361 39566 6.3 U PSA1 SGDID:S000002213, Chr IV from 356759-355674, reverse complement, Verified ORF, "GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure" * 22_g_01_itms.15137.15137.2 4.7059 0.4211 100.0 2291.652 2291.0693 10103.3 1 7.359 58.3 3 K.DNSPFFVLNSDVICEYPFK.E * 22_g_01_itms.15098.15098.3 4.2264 0.1964 99.0 2291.9644 2291.0693 7731.4 1 4.646 40.3 1 K.DNSPFFVLNSDVICEYPFK.E * 22_g_01_itms.08313.08313.2 2.3667 0.2202 97.8 1205.2522 1204.6465 7933.7 67 4.785 61.1 2 K.ETFPILVEEK.Q * 22_g_01_itms.15830.15830.2 4.8308 0.409 100.0 1641.3922 1640.8899 8250.8 1 8.072 75.0 2 K.DFLSGTVLYLNSLAK.R * 22_g_01_itms.10046.10046.2 3.4551 0.2925 100.0 1753.6122 1750.8335 8920.6 1 6.343 53.6 2 K.STIVGWNSTVGQWCR.L * 22_g_01_itms.26659.26659.3 3.696 0.1115 93.4 2464.8542 2462.2666 7136.8 8 3.973 30.7 1 R.LEGVTVLGDDVEVKDEIYINGGK.V YER112W 1 1 22.5% 187 21276 9.5 U LSM4 SGDID:S000000914, Chr V from 387228-387791, Verified ORF, "Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA" * 22_g_01_itms.25249.25249.3 3.933 0.277 100.0 4632.534 4629.1426 10129.0 3 5.354 17.7 1 K.NGEIIQGILTNVDNWMNLTLSNVTEYSEESAINSEDNAESSK.A YIL149C 31 75 22.2% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * 22_g_01_itms.15391.15391.2 2.7732 0.2239 100.0 2412.4321 2408.2866 9157.5 19 5.55 32.5 1 K.ISEFLNVPFESLQGVTYPVLR.K * 22_g_01_itms.04802.04802.2 3.6828 0.3276 100.0 1822.3322 1818.8105 6014.4 2 5.797 50.0 4 R.DQGNDSLNDDLNKENK.L * 22_g_01_itms.04802.04802.3 3.7899 0.176 98.3 2732.9944 2729.4004 6014.4 15 4.345 28.4 1 R.DQGNDSLNDDLNKENKLLRRKLM.E * 22_g_01_itms.45486.45486.3 2.9511 0.1379 92.9 2601.4443 2600.1536 12047.2 48 3.931 29.8 1 K.NDDNSCRNPEHTDVIDELIDTK.L * 22_g_01_itms.08289.08289.2 3.616 0.3308 100.0 2116.392 2113.9456 5367.8 1 6.674 47.1 3 R.LQNIVMDCTKEEEATMTT.S * 22_g_01_itms.14390.14390.2 4.304 0.3838 100.0 2806.9922 2805.465 9722.5 2 6.126 33.3 2 K.LLLLNTSAIQETAS*PLSQDELISLR.K * 22_g_01_itms.14414.14414.3 5.1624 0.3052 100.0 2807.4243 2805.465 7881.9 1 6.379 36.5 1 K.LLLLNTSAIQETAS*PLSQDELISLR.K * 22_g_01_itms.07058.07058.2 3.3841 0.0972 92.8 2246.7122 2245.131 7178.3 1 4.39 42.1 2 K.ILESSNIVNENDSQAIITER.L * 22_g_01_itms.07007.07007.2 4.2026 0.2755 100.0 1550.4122 1548.791 7370.5 1 6.011 70.8 3 R.LVEFSNVNELQEK.N * 22_g_01_itms.08297.08297.2 4.8218 0.232 100.0 1343.3922 1342.7218 8894.8 1 5.872 81.8 6 K.DAIIELENINAK.M * 22_g_01_itms.04377.04377.2 2.2165 0.3416 100.0 1106.9722 1106.5581 5466.9 1 5.644 77.8 2 R.ELEAELSSTK.V * 22_g_01_itms.08846.08846.2 2.6812 0.2256 95.4 1245.4521 1243.5846 6923.2 1 4.496 77.8 4 K.TTLEDFENFK.G * 22_g_01_itms.07454.07454.2 3.2208 0.2181 96.9 1808.4122 1806.8873 10020.5 6 4.743 46.7 2 K.FLDQNSDASTLEPTLR.K * 22_g_01_itms.15867.15867.3 5.8 0.3372 100.0 2693.3342 2692.3318 11545.6 1 6.549 40.2 4 K.DANSQIQAYEEIISSNENALIELK.N * 22_g_01_itms.15876.15876.2 5.3358 0.3663 100.0 2694.5522 2692.3318 6963.7 1 6.341 52.2 3 K.DANSQIQAYEEIISSNENALIELK.N * 22_g_01_itms.20158.20158.3 4.4004 0.2065 99.7 3249.4744 3247.6333 8272.9 104 5.041 20.5 2 K.DANSQIQAYEEIISSNENALIELKNELAK.T * 22_g_01_itms.00870.00870.2 3.6929 0.3404 100.0 1222.0922 1221.5421 9790.1 1 7.088 85.0 6 K.DAADCQAELTK.T * 22_g_01_itms.07670.07670.2 4.2895 0.3444 100.0 1533.0721 1531.7968 7407.5 2 5.961 70.8 2 R.ELISNIEQTESLR.V * 22_g_01_itms.08023.08023.2 2.9239 0.3218 100.0 1472.2322 1471.7756 5838.7 14 5.792 54.2 3 K.QQAALTNELNELK.A * 22_g_01_itms.07220.07220.2 2.944 0.3366 100.0 1310.4722 1308.6436 5298.5 1 5.996 63.6 1 K.ENAGSLTFLDNK.G * 22_g_01_itms.11105.11105.3 4.2482 0.2167 94.6 3106.3145 3104.3374 8140.2 32 4.858 22.2 2 K.ENAGSLTFLDNKGS*GEDAEEELWNSPSK.G * 22_g_01_itms.06503.06503.2 5.1361 0.3484 100.0 1735.2722 1734.7458 11428.4 1 6.611 70.0 2 K.GSGEDAEEELWNSPSK.G * 22_g_01_itms.18960.18960.3 4.504 0.3053 100.0 3471.2644 3470.55 4532.0 87 5.356 23.4 3 K.GSGEDAEEELWNSPS*KGNSERPSAVAGFINQK.N * 22_g_01_itms.00423.00423.2 2.616 0.1448 98.4 1319.8922 1318.6239 9465.1 6 4.892 58.3 2 K.ENNIVDSSAAGNK.A * 22_g_01_itms.13499.13499.2 2.5512 0.2734 99.3 1533.2722 1532.7789 4691.6 201 5.158 50.0 1 K.AIPTFSFGKPFFSS.N * 22_g_01_itms.13188.13188.2 3.1156 0.0876 99.3 2164.3523 2163.0762 6372.7 30 5.084 34.2 2 K.AIPTFSFGKPFFSSNTSSLQ.S * 22_g_01_itms.10529.10529.3 3.5149 0.1757 91.1 2840.3044 2837.3818 9787.3 2 3.837 27.0 1 S.NTSSLQSFQNPFTASQSNINTNAPLR.T * 22_g_01_itms.08259.08259.2 4.7113 0.3123 100.0 2054.3123 2052.984 8206.4 1 6.212 52.6 3 K.AAINFSNVTDLTNNSTDGAK.I * 22_g_01_itms.08376.08376.3 4.3756 0.1553 96.3 2054.9343 2052.984 7734.8 9 4.179 35.5 1 K.AAINFSNVTDLTNNSTDGAK.I * 22_g_01_itms.08229.08229.2 4.6309 0.4287 100.0 1982.9521 1981.9467 7321.0 1 7.74 58.3 3 A.AINFSNVTDLTNNSTDGAK.I * 22_g_01_itms.07867.07867.2 4.5654 0.3958 100.0 1913.7722 1910.9095 8955.7 1 7.634 64.7 2 A.INFSNVTDLTNNSTDGAK.I YDL229W 11 58 22.0% 613 66602 5.4 U SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; interacts with the phosphatase subunit Reg1p" YNL209W 11 58 22.0% 613 66595 5.5 U SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; homolog of SSB1" 22_g_01_itms.11534.11534.2 4.9863 0.4196 100.0 2161.172 2160.1077 6902.2 1 8.755 61.1 2 K.VIDVDGNPVIEVQYLEETK.T 22_g_01_itms.07961.07961.2 3.4499 0.1961 100.0 1185.5521 1185.6592 5929.6 1 5.577 86.4 3 K.DAGAISGLNVLR.I 22_g_01_itms.15785.15785.3 5.1956 0.4246 100.0 2964.0842 2963.401 9067.2 1 6.987 30.8 6 R.TLSSVTQTTVEVDSLFDGEDFESSLTR.A 22_g_01_itms.15828.15828.2 4.5336 0.4411 100.0 2965.4922 2963.401 7802.1 1 7.32 40.4 5 R.TLSSVTQTTVEVDSLFDGEDFESSLTR.A 22_g_01_itms.13697.13697.2 4.1069 0.4967 100.0 1990.2122 1989.8817 7235.1 1 8.756 64.7 2 T.TVEVDSLFDGEDFESSLT.R 22_g_01_itms.11113.11113.2 2.8723 0.2121 100.0 1279.2522 1278.6582 6321.5 1 5.493 80.0 2 K.ENTLLGEFDLK.N 22_g_01_itms.19358.19358.3 3.7746 0.2994 100.0 2356.1943 2355.0662 5160.8 1 5.78 38.8 14 L.SSEEIEKMVNQAEEFKAADEA.F 22_g_01_itms.12583.12583.2 4.5079 0.4078 100.0 2165.7722 2166.1182 10060.4 1 8.681 44.7 3 R.LESYVASIEQTVTDPVLSSK.L 22_g_01_itms.28108.28108.3 5.1296 0.2267 99.7 2629.0144 2626.3577 9057.9 1 4.84 33.3 15 K.SKIEAALSDALAALQIEDPSADELR.K 22_g_01_itms.14610.14610.3 5.3409 0.1419 99.0 2412.5044 2411.2305 7909.4 1 4.587 39.8 2 K.IEAALSDALAALQIEDPSADELR.K 22_g_01_itms.14634.14634.2 6.2604 0.4636 100.0 2415.652 2411.2305 9065.6 1 7.717 54.5 4 K.IEAALSDALAALQIEDPSADELR.K YNL064C 2 65 19.1% 409 44671 6.3 U YDJ1 SGDID:S000005008, Chr XIV from 507098-505869, reverse complement, Verified ORF, "Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein translocation across membranes; member of the DnaJ family" * 22_g_01_itms.22643.22643.3 3.9564 0.248 98.3 4461.8945 4459.0083 7378.8 9 4.357 17.0 2 R.DIYDQFGEDGLSGAGGAGGFPGGGFGFGDDIFSQFFGAGGAQRPR.G * 22_g_01_itms.29316.29316.3 7.5798 0.4272 100.0 3566.0044 3561.7278 4374.9 1 7.583 28.9 63 R.DGDDLVYEAEIDLLTAIAGGEFALEHVSGDWLK.V YAL005C 9 17 19.0% 642 69768 5.1 U SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall" * 22_g_01_itms.05347.05347.2 3.318 0.2386 100.0 1407.1522 1407.6215 8604.1 2 5.261 72.7 1 R.NFNDPEVQADMK.H 22_g_01_itms.14927.14927.3 3.2954 0.3067 99.7 1677.0844 1675.7312 4924.6 1 4.883 41.7 2 K.ATAGDTHLGGEDFDNR.L 22_g_01_itms.14049.14049.3 3.5944 0.3016 100.0 2578.3145 2577.2683 9710.3 3 5.176 28.0 2 R.SINPDEAVAYGAAVQAAILTGDESSK.T 22_g_01_itms.14083.14083.2 5.3564 0.5322 100.0 2581.8323 2577.2683 10901.7 1 8.995 44.0 3 R.SINPDEAVAYGAAVQAAILTGDESSK.T 22_g_01_itms.09411.09411.2 2.6828 0.1985 99.6 1265.7522 1265.6742 8448.0 42 5.213 65.0 2 K.NQLESIAYSLK.N * 22_g_01_itms.45905.45905.3 2.6218 0.1598 92.1 2165.3643 2163.0781 7001.3 114 3.901 28.9 1 K.NTISEAGDKLEQADKDTVTK.K * 22_g_01_itms.06689.06689.3 3.1642 0.0244 98.6 2438.1243 2436.122 6966.7 279 4.447 23.2 1 L.YQAGGAPGGAAGGAPGGFPGGAPPAPEAE.G * 22_g_01_itms.08483.08483.3 3.0002 0.2029 99.0 3262.8245 3262.493 7040.0 402 4.628 18.8 1 L.YQAGGAPGGAAGGAPGGFPGGAPPAPEAEGPTVEEVD.- 22_g_01_itms.08339.08339.2 3.5598 0.3224 100.0 2178.7722 2177.004 6132.1 27 6.138 34.1 4 A.PGGFPGGAPPAPEAEGPTVEEVD.- YLR048W 5 10 18.7% 252 27962 4.7 U RPS0B SGDID:S000004038, Chr XII from 242233-242322,242682-243350, Verified ORF, "Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal" * 22_g_01_itms.13999.13999.3 3.4042 0.1922 93.4 3764.5745 3759.6367 9566.6 2 4.014 20.6 1 R.NPEEVEQVAEEAAAAEEGEEEEVKEEVTEGQAEAT.E * 22_g_01_itms.19388.19388.3 6.1544 0.5481 100.0 5235.2046 5232.221 8324.3 1 8.759 23.4 4 R.NPEEVEQVAEEAAAAEEGEEEEVKEEVTEGQAEATEWAEENADNVEW.- * 22_g_01_itms.16548.16548.3 4.8838 0.3328 100.0 3910.6143 3907.643 9348.0 3 5.993 20.6 2 A.AAAEEGEEEEVKEEVTEGQAEATEWAEENADNVEW.- 22_g_01_itms.09792.09792.2 4.431 0.4645 100.0 1865.4722 1863.7673 10069.5 1 8.339 66.7 1 Q.AEATEWAEENADNVEW.- 22_g_01_itms.08675.08675.2 3.5271 0.2387 100.0 1493.0322 1491.6028 6664.8 1 6.058 77.3 2 T.EWAEENADNVEW.- YOR383C 1 1 18.6% 204 19836 4.3 U FIT3 SGDID:S000005910, Chr XV from 1061051-1060437, reverse complement, Verified ORF, "Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall" * 22_g_01_itms.13075.13075.3 3.4891 0.2306 98.7 3412.3743 3408.4897 6680.8 240 4.387 17.6 1 A.AESSAAETSAAETSAAATTSAAATTSAAETSSAAETSS.A YNL225C 9 26 17.6% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * 22_g_01_itms.15241.15241.2 4.463 0.3188 100.0 2420.5723 2419.208 7960.2 1 6.68 52.5 3 K.SILPLFVNPEDVINCNFSNAR.D * 22_g_01_itms.15302.15302.3 5.8382 0.423 100.0 2421.6243 2419.208 8048.4 1 7.27 43.8 2 K.SILPLFVNPEDVINCNFSNAR.D * 22_g_01_itms.15522.15522.2 4.4531 0.2775 100.0 2500.6921 2499.1743 8440.2 1 6.001 50.0 2 K.SILPLFVNPEDVINCNFS*NAR.D * 22_g_01_itms.15549.15549.3 4.2536 0.2387 94.0 2501.8145 2499.1743 6575.9 18 4.815 30.0 1 K.SILPLFVNPEDVINCNFS*NAR.D * 22_g_01_itms.10037.10037.2 3.2095 0.2759 100.0 1896.0922 1895.871 7651.6 1 5.655 56.7 1 L.FVNPEDVINCNFSNAR.D * 22_g_01_itms.11238.11238.3 3.3355 0.1906 98.6 2167.1943 2164.8916 7586.2 13 4.415 35.3 1 R.DSYEENKSPSMDQMNYAR.N * 22_g_01_itms.04784.04784.3 3.8144 0.2856 94.7 3046.5544 3045.1821 7960.0 3 4.848 25.0 1 R.ANEQASAQPTDEHTS*PDIS*IEDCNGAK.I * 22_g_01_itms.08843.08843.2 2.2653 0.2218 97.0 1308.9321 1307.7034 6417.7 6 4.727 65.0 1 R.MLENVILGYQK.K * 22_g_01_itms.30027.30027.3 4.8243 0.2362 100.0 2907.6543 2904.4558 9481.7 1 5.814 31.2 14 R.KAELFEIPIGILFFDLYDSEENSSK.L Reverse_YFR011C 1 1 17.6% 170 18855 5.5 U YFR011C SGDID:S000001907, Chr VI from 167251-166739, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.39284.39284.3 3.1638 0.1861 98.3 3211.0144 3206.8113 6064.1 19 4.376 22.4 1 E.TLKAKISISGNDTKKDSKGKAGGYKELKQL.E YAL038W 6 33 17.0% 500 54545 7.7 U CDC19 SGDID:S000000036, Chr I from 71787-73289, Verified ORF, "Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration" * 22_g_01_itms.21477.21477.2 3.0632 0.2043 92.6 2266.5322 2266.1858 7083.9 279 4.378 34.2 1 R.IIYVDDGVLSFQVLEVVDDK.T * 22_g_01_itms.10305.10305.2 4.0672 0.3816 100.0 1727.4922 1724.9071 5302.8 1 7.134 62.5 2 K.GVNLPGTDVDLPALSEK.D * 22_g_01_itms.11580.11580.2 3.565 0.1908 98.4 1863.0721 1860.8762 10104.0 5 4.842 44.1 1 T.RAEVSDVGNAILDGADCV.M * 22_g_01_itms.13170.13170.2 4.8809 0.4079 100.0 2522.8323 2522.1755 8105.8 1 7.522 45.8 1 R.AEVSDVGNAILDGADCVMLSGETAK.G * 22_g_01_itms.18577.18577.3 5.6015 0.3097 100.0 2570.2144 2569.225 7494.2 1 7.213 47.6 27 R.GVFPFVFEKEPVSDWTDDVEAR.I * 22_g_01_itms.06605.06605.2 2.8039 0.3021 100.0 1519.1522 1518.6713 7670.1 2 6.184 50.0 1 K.EPVSDWTDDVEAR.I YPL059W 1 18 16.7% 150 16931 4.9 U GRX5 SGDID:S000005980, Chr XVI from 444576-445028, Verified ORF, "Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; mitochondrial matrix protein involved in the synthesis/assembly of iron-sulfur centers; monothiol glutaredoxin subfamily member along with Grx3p and Grx4p" * 22_g_01_itms.20202.20202.3 4.8913 0.2182 99.7 2803.0745 2800.3376 7016.4 1 4.892 31.2 18 R.SGELADLLEEAQALVPEEEEETKDR.- YLR180W 3 22 15.7% 382 41818 5.2 U SAM1 SGDID:S000004170, Chr XII from 515264-516412, Verified ORF, "S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)" * 22_g_01_itms.18876.18876.3 6.1572 0.44 100.0 3603.7144 3600.7783 5655.5 1 7.122 28.8 11 K.DLEDIGAGDQGIMFGYATDETPEGLPLTILLAHK.L 22_g_01_itms.07227.07227.2 4.2008 0.3889 100.0 1447.4722 1444.7549 7510.1 1 6.743 78.6 7 R.FVIGGPQGDAGLTGR.K * 22_g_01_itms.09995.09995.2 3.9404 0.2059 98.4 1262.3121 1261.6527 6413.3 1 4.831 85.0 4 K.SDEEIIDIISK.N Reverse_YPL229W 1 1 15.5% 206 23260 6.3 U YPL229W SGDID:S000006150, Chr XVI from 117067-117687, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.26205.26205.3 3.3892 0.174 97.7 3377.6343 3379.5361 6887.1 6 4.279 22.6 1 P.NFKPRSNFISSASNPPSSSMSGPNSSSQTNQH.Y Reverse_YOL109W 1 1 15.0% 113 12589 5.4 U ZEO1 SGDID:S000005469, Chr XV from 110296-110637, Verified ORF, "Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p" * 22_g_01_itms.34209.34209.3 2.3717 0.094 91.3 1963.9143 1960.0715 6771.1 262 3.851 32.8 1 Q.KKTEEVKNAAETKKQEV.G YKL159C 1 1 14.7% 211 24135 5.5 U RCN1 SGDID:S000001642, Chr XI from 154456-153821, reverse complement, Verified ORF, "Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region" * 22_g_01_itms.45829.45829.3 1.7223 0.0744 90.6 3470.5745 3473.6582 6127.6 215 3.81 13.3 1 T.IDRCPTNDGNGQMQLADHVKTAFPPKSIFDT.D YLR061W 1 2 14.0% 121 13693 6.2 U RPL22A SGDID:S000004051, Chr XII from 263195-263206,263596-263949, Verified ORF, "Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Bp and to rat L22 ribosomal protein" * 22_g_01_itms.08160.08160.2 3.9318 0.3198 100.0 2072.132 2071.8508 5651.8 1 7.018 62.5 2 R.LAFYQVTPEEDEEEDEE.- YOR261C 2 10 13.9% 338 38313 5.6 U RPN8 SGDID:S000005787, Chr XV from 816929-815913, reverse complement, Verified ORF, "Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p" * 22_g_01_itms.17825.17825.3 5.3533 0.313 100.0 2995.6743 2993.4128 6118.3 164 5.23 26.0 8 K.LQDVFNLLPNLGTPDDDEIDVENHDR.I * 22_g_01_itms.11133.11133.3 3.7461 0.252 99.7 2235.0842 2234.0425 8899.5 1 4.893 38.8 2 K.VSDDSESESGDKEATAPLIQR.K YOR369C 1 5 13.3% 143 15472 4.7 U RPS12 SGDID:S000005896, Chr XV from 1028621-1028190, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12" * 22_g_01_itms.16436.16436.2 4.604 0.3053 100.0 2256.5923 2252.9592 5778.5 1 6.015 55.6 5 K.NWGAETDELSMIMEHFSQQ.- YOR083W 1 1 13.2% 295 32901 6.8 U WHI5 SGDID:S000005609, Chr XV from 479534-480421, Verified ORF, "Protein that regulates the critical cell size required for passage through Start and commitment to cell division; may act upstream of SCB binding factor (SBF) and MCB binding factor (MBF); periodically expressed in G1" * 22_g_01_itms.18868.18868.3 4.222 0.3041 100.0 4292.8145 4292.2686 6187.3 272 5.577 17.1 1 T.LLSTPVRLKNGFGTPS*PPS*PPGITKSITKSRRRPSTTSL.Q YDL004W 1 1 13.1% 160 17020 6.5 U ATP16 SGDID:S000002162, Chr IV from 443026-443508, Verified ORF, "Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis" * 22_g_01_itms.08061.08061.2 2.6764 0.158 90.5 2111.2522 2110.162 7989.7 31 4.313 35.0 1 S.GSEVTQVNLPAKSGRIGVLAN.H YDR502C 2 12 12.8% 384 42256 5.4 U SAM2 SGDID:S000002910, Chr IV from 1454454-1453300, reverse complement, Verified ORF, "S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)" * 22_g_01_itms.19603.19603.3 4.3684 0.2417 99.0 3577.6143 3572.7834 8263.1 2 4.558 22.7 5 K.SLEDLGAGDQGIMFGYATDETPEGLPLTILLAHK.L 22_g_01_itms.07227.07227.2 4.2008 0.3889 100.0 1447.4722 1444.7549 7510.1 1 6.743 78.6 7 R.FVIGGPQGDAGLTGR.K YLR340W 1 1 12.8% 312 33717 4.8 U RPP0 SGDID:S000004332, Chr XII from 805887-806825, Verified ORF, "Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphorylated on serine 302" * 22_g_01_itms.14433.14433.3 6.5308 0.4629 100.0 4007.6042 4000.5754 6996.7 1 8.23 23.7 1 K.YAAAAPAATSAASGDAAPAEEAAAEEEEES*DDDMGFGLFD.- YHR051W 1 1 12.8% 148 17342 6.0 U COX6 SGDID:S000001093, Chr VIII from 209699-210145, Verified ORF, "Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels" * 22_g_01_itms.11051.11051.2 4.394 0.4368 100.0 2150.912 2149.1003 5315.3 1 6.874 50.0 1 R.VLNNCFSYDLVPAPAVIEK.A YER095W 2 2 12.5% 400 42963 4.9 U RAD51 SGDID:S000000897, Chr V from 349976-351178, Verified ORF, "Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein" * 22_g_01_itms.11819.11819.2 2.385 0.2139 95.4 2301.892 2300.028 9849.5 30 4.522 32.5 1 Q.GEMEDEAYDEAALGSFVPIEK.L * 22_g_01_itms.14085.14085.3 3.7655 0.2217 98.7 3298.0745 3296.425 9334.4 8 4.398 22.3 1 K.VVDSPCLPEAECVFAIYEDGVGDPREEDE.- YAL044C 1 1 12.4% 170 18795 4.7 U GCV3 SGDID:S000000042, Chr I from 58463-57951, reverse complement, Verified ORF, "H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm" * 22_g_01_itms.12854.12854.2 2.8892 0.2398 92.2 2354.2922 2351.144 9172.2 9 4.374 35.0 1 K.LGEGVNVEQVEGLMSLEQYEK.T YLR437C 1 1 12.0% 133 15165 7.4 U YLR437C SGDID:S000004429, Chr XII from 1012019-1011618, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.12355.12355.2 1.9083 0.2093 95.5 1706.0922 1705.8397 4299.9 9 4.487 40.0 1 T.PSTSDASLQPGVIRDY.S YDR418W 1 4 11.5% 165 17823 9.4 U RPL12B SGDID:S000002826, Chr IV from 1301606-1302103, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins" YEL054C 1 4 11.5% 165 17823 9.4 U RPL12A SGDID:S000000780, Chr V from 53218-52721, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Bp; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins" 22_g_01_itms.11397.11397.2 3.8724 0.2495 99.3 2076.3123 2074.0093 5285.8 2 4.944 47.2 4 K.NPHDIIEGINAGEIEIPEN.- Reverse_YAR002C-A 1 1 11.4% 219 24723 7.4 U ERP1 SGDID:S000002129, Chr I from 154726-154067, reverse complement, Verified ORF, "Protein that forms a heterotrimeric complex with Erp2p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles" * 22_g_01_itms.36952.36952.2 1.7514 0.1601 93.1 2567.2122 2570.2124 11168.3 14 4.4 31.2 1 E.GSDSALFTLDGSASGKQHVVLHNDD.F YOR117W 2 3 11.1% 434 48256 5.1 U RPT5 SGDID:S000005643, Chr XV from 545029-546333, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription" * 22_g_01_itms.13189.13189.2 3.0457 0.2336 95.4 1871.3722 1870.9075 6862.8 19 4.511 43.3 2 K.DSYLILDTLPSEFDSR.V * 22_g_01_itms.21575.21575.3 4.2294 0.0122 92.2 3494.2144 3489.755 6429.4 63 3.909 21.0 1 I.NWQELARSTDEFNGAQLKAVTVEAGMIALRNG.Q YDR356W 10 28 11.0% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * 22_g_01_itms.09235.09235.2 2.8455 0.2928 95.0 1749.8722 1749.7584 7998.4 254 5.212 39.3 1 R.SIDDTIDS*TRLFSEA.S * 22_g_01_itms.10850.10850.2 4.5036 0.3785 100.0 1861.1322 1859.8704 9244.0 1 6.822 70.0 2 R.LFSEASQFDDSFPEIK.A * 22_g_01_itms.11663.11663.2 4.151 0.3357 100.0 1940.5521 1939.8367 6658.2 1 6.271 60.0 1 R.LFSEAS*QFDDSFPEIK.A * 22_g_01_itms.10793.10793.2 4.2933 0.2405 94.7 1943.3322 1939.8367 6923.3 1 5.318 63.3 4 R.LFSEASQFDDS*FPEIK.A * 22_g_01_itms.15788.15788.3 4.6739 0.163 98.3 2401.6443 2399.0737 9779.4 2 4.34 38.2 5 K.EKNDTLNNYDTLEEETDDLK.N * 22_g_01_itms.08657.08657.2 5.4847 0.3799 100.0 2143.2522 2141.9363 9690.7 1 7.155 67.6 7 K.NDTLNNYDTLEEETDDLK.N * 22_g_01_itms.07325.07325.2 5.67 0.3763 100.0 1832.2122 1830.9086 9554.0 1 6.945 73.3 4 K.DQVLELENNSDVQSLK.L * 22_g_01_itms.06959.06959.2 3.1516 0.1659 95.8 1404.1322 1403.7018 7798.4 3 4.577 68.2 1 K.DEQISDLTQNLK.L * 22_g_01_itms.10251.10251.2 4.3178 0.418 100.0 1696.6522 1695.819 9342.0 1 7.539 61.5 1 K.NYLSEITSLQEENR.R * 22_g_01_itms.11723.11723.2 4.5529 0.3903 100.0 1920.5322 1919.8696 9975.7 1 7.071 80.0 2 K.DNDSTMQLNDIISYYK.L YBR287W 2 2 11.0% 427 47506 4.9 U YBR287W SGDID:S000000491, Chr II from 776567-777850, Uncharacterized ORF, "Protein of unknown function; mutation results in a zinc sensitive phenotype" * 22_g_01_itms.10097.10097.3 3.8846 0.2193 99.7 3156.5942 3156.4092 10383.7 2 4.987 25.0 1 R.TPNIDNEELVNEEQEEQELLEEENNR.M * 22_g_01_itms.22609.22609.2 2.323 0.1399 95.4 2263.0122 2259.3005 8633.6 55 4.504 30.0 1 I.AIAVKYINVSILDDPIFLVVG.F YLR216C 2 15 10.2% 371 42072 6.2 U CPR6 SGDID:S000004206, Chr XII from 573213-572098, reverse complement, Verified ORF, "Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity" * 22_g_01_itms.10847.10847.3 4.9796 0.1826 99.4 3866.5444 3859.6655 5253.2 1 4.815 31.8 2 K.IDDCGVLPDDYQVPENAEATPTDEYGDNYEDVLK.Q * 22_g_01_itms.12527.12527.3 5.6166 0.3579 100.0 4363.344 4359.8887 5742.6 1 6.756 26.4 13 K.IDDCGVLPDDYQVPENAEATPTDEYGDNYEDVLKQDEK.V YNR054C 1 1 10.1% 316 36408 8.0 U ESF2 SGDID:S000005337, Chr XIV from 724307-723357, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; component of the small subunit (SSU) processome; has sequence similarity to mABT1, a mouse transcription activator" * 22_g_01_itms.11793.11793.3 2.8365 0.0522 90.7 3764.1243 3758.84 6893.8 417 3.807 19.4 1 D.FEDFSSDEETDQHNVLIQTKKKISSKDDIFSK.K Reverse_YMR263W 1 1 10.0% 201 23026 9.7 U SAP30 SGDID:S000004876, Chr XIII from 794918-795523, Verified ORF, "Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci" * 22_g_01_itms.23224.23224.2 2.2489 0.2119 96.4 2009.6322 2009.9026 7795.7 56 4.691 31.6 1 C.SGNNNSAYGGGQTPRGRSET.E YJR053W 3 5 9.9% 574 66087 5.9 U BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" * 22_g_01_itms.10406.10406.2 3.9608 0.2608 96.9 2457.172 2456.1233 9254.2 1 5.527 40.0 2 L.TLNGLDEPETS*FEELNTTLPR.F * 22_g_01_itms.12462.12462.3 6.5513 0.4498 100.0 3184.0444 3178.2708 8483.2 1 7.353 32.0 2 K.QADDDQDMEVDQDDEFLNDFQEFQNK.K * 22_g_01_itms.08964.08964.2 2.6814 0.212 96.0 1256.4722 1255.5594 6713.3 1 4.628 77.8 1 K.NDIFAEEFDR.K YHL034C 2 53 9.9% 294 32990 5.7 U SBP1 SGDID:S000001026, Chr VIII from 34075-33191, reverse complement, Verified ORF, "Nucleolar single-strand nucleic acid binding protein; associates with small nuclear RNAs" * 22_g_01_itms.13310.13310.3 6.134 0.3779 100.0 3350.9043 3344.5505 8616.6 1 6.292 30.4 31 R.ELTVDVAVIRPENDEEEIEQETGSEEKQE.- * 22_g_01_itms.13381.13381.3 4.5071 0.0563 99.7 2618.1543 2617.1753 7311.3 1 5.068 38.1 22 A.VIRPENDEEEIEQETGSEEKQE.- YKL056C 1 1 9.6% 167 18741 4.6 U YKL056C SGDID:S000001539, Chr XI from 334559-334056, reverse complement, Verified ORF, "Protein of unknown function; putative ribosomal protein; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YKL056C is not an essential gene" * 22_g_01_itms.10986.10986.2 4.5829 0.4293 100.0 1817.3922 1815.8289 9382.3 1 7.028 60.0 1 K.DIFSNDELLSDAYDAK.L YMR244W 1 1 9.3% 355 37383 5.3 U YMR244W SGDID:S000004858, Chr XIII from 757249-758316, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.08142.08142.3 2.7535 0.1531 90.2 3107.2144 3111.4038 9060.0 8 3.799 20.3 1 S.IDPSSNGVNEVTSSGGGSSGAGGGNFCVVTARN.G Reverse_YDR530C 1 1 9.2% 325 36841 5.5 U APA2 SGDID:S000002938, Chr IV from 1497758-1496781, reverse complement, Verified ORF, "Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase II (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa1p" * 22_g_01_itms.34920.34920.3 3.1687 0.171 95.6 3650.0942 3656.9116 11103.2 69 4.151 23.3 1 C.IWKKTLLVNYSKTLEPSENTWDQFFTLARQ.M YJL034W 6 21 9.1% 682 74468 4.9 U KAR2 SGDID:S000003571, Chr X from 381243-383291, Verified ORF, "ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p" 22_g_01_itms.14409.14409.2 3.0215 0.1122 97.0 2025.5922 2023.0236 9118.6 16 4.724 41.2 2 R.IEIDSFVDGIDLSETLTR.A 22_g_01_itms.07314.07314.2 2.6626 0.2655 98.4 1347.0721 1345.6963 4567.1 1 4.834 66.7 1 K.DVDDIVLVGGSTR.I 22_g_01_itms.16739.16739.3 4.833 0.2418 99.7 3401.9644 3398.2893 6816.5 5 4.886 23.3 8 S.KLYGGADGSGAADYDDEDEDDDGDYFEHDEL.- 22_g_01_itms.09791.09791.3 7.4408 0.5142 100.0 3271.3442 3270.1943 8399.1 1 8.913 34.5 5 K.LYGGADGSGAADYDDEDEDDDGDYFEHDEL.- 22_g_01_itms.09648.09648.2 4.9448 0.4616 100.0 3272.392 3270.1943 11044.9 1 9.284 34.5 3 K.LYGGADGSGAADYDDEDEDDDGDYFEHDEL.- 22_g_01_itms.09405.09405.3 4.2404 0.1346 99.0 3160.1042 3157.1104 8146.1 1 4.577 26.8 2 L.YGGADGSGAADYDDEDEDDDGDYFEHDEL.- YBR162C 1 1 9.0% 455 47994 4.7 U TOS1 SGDID:S000000366, Chr II from 564565-563198, reverse complement, Uncharacterized ORF, "Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C" * 22_g_01_itms.29172.29172.3 3.0377 0.032 92.3 4088.6643 4086.9478 9249.2 6 3.905 17.5 1 N.GQTVTADSTNTVVGPAVPSSYTKDSTVLSSSAQAVETSESQ.S Reverse_YLR312C 1 1 9.0% 398 46628 9.7 U YLR312C SGDID:S000004303, Chr XII from 758835-757639, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.18888.18888.3 4.7716 0.2107 90.6 3608.5444 3603.6414 6789.8 3 4.75 24.3 1 K.SGEMSPLVTGGVNSGS*LTDFSESITPVGTQDINVGA.N YCL050C 1 17 9.0% 321 36493 5.0 U APA1 SGDID:S000000555, Chr III from 38801-37836, reverse complement, Verified ORF, "Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p" * 22_g_01_itms.15419.15419.3 4.7316 0.3731 100.0 3055.0745 3053.4592 5554.5 1 6.602 28.6 17 R.GQTPEGEDPLGKPEEELTVIPEFGGADNK.A YBL027W 1 1 9.0% 189 21704 11.4 U RPL19B SGDID:S000000123, Chr II from 168426-168427,168812-169379, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Bp and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal" YBR084C-A 1 1 9.0% 189 21704 11.4 U RPL19A SGDID:S000002156, Chr II from 415255-415254,414747-414180, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Bp and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal" 22_g_01_itms.07953.07953.2 2.8068 0.3006 100.0 1932.5721 1929.9307 6374.8 4 5.493 43.8 1 K.VWLDPNETSEIAQANSR.N Reverse_YDR044W 1 1 8.8% 328 37712 6.8 U HEM13 SGDID:S000002451, Chr IV from 546639-547625, Verified ORF, "Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; localizes to the mitochondrial inner membrane; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)" * 22_g_01_itms.24692.24692.3 4.1139 0.1692 96.8 3405.8643 3399.6133 8773.4 71 4.219 20.5 1 A.TDHKDLADKHLQHFLQGDEEYLYSPTLDA.G YBR118W 2 2 8.7% 458 50033 9.0 U TEF2 SGDID:S000000322, Chr II from 477665-479041, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" YPR080W 2 2 8.7% 458 50033 9.0 U TEF1 SGDID:S000006284, Chr XVI from 700592-701968, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" 22_g_01_itms.28984.28984.3 3.2343 0.0855 93.2 3120.3542 3120.5464 9050.9 265 3.963 19.6 1 K.KVGYNPKTVPFVPISGWNGDNMIEATTNA.P 22_g_01_itms.07063.07063.2 2.5411 0.2562 99.3 1267.6322 1265.6166 7709.9 5 5.133 65.0 1 V.EAFSEYPPLGR.F YOR051C 2 6 8.7% 412 47352 4.8 U YOR051C SGDID:S000005577, Chr XV from 426085-424847, reverse complement, Uncharacterized ORF, "Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts" * 22_g_01_itms.26800.26800.3 4.7384 0.3546 100.0 4087.5244 4081.9043 11252.5 1 6.653 22.1 5 K.AKLEDDPDTWVQVAEAYIDLGNLLDNESAEQEEAYK.T * 22_g_01_itms.20830.20830.3 6.3746 0.3483 100.0 3889.7644 3882.7722 12360.4 1 7.019 27.3 1 K.LEDDPDTWVQVAEAYIDLGNLLDNESAEQEEAYK.T YBL039C 2 17 8.6% 579 64710 6.0 U URA7 SGDID:S000000135, Chr II from 145731-143992, reverse complement, Verified ORF, "Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis" 22_g_01_itms.17465.17465.3 5.8605 0.3524 100.0 4263.6543 4258.853 7397.2 1 6.316 23.0 16 K.IDPYMNIDAGTMSPLEHGECFVLDDGGETDLDLGNYER.Y * 22_g_01_itms.09530.09530.2 3.3351 0.2197 100.0 1447.4321 1446.6542 8798.1 1 5.32 81.8 1 K.YDLEAGENKFNF.- YNL113W 1 2 8.5% 142 16151 4.4 U RPC19 SGDID:S000005057, Chr XIV from 412773-413201, Verified ORF, "RNA polymerase subunit, common to RNA polymerases I and III" * 22_g_01_itms.10049.10049.2 3.5162 0.3259 100.0 1424.9722 1423.6449 8032.3 1 5.836 72.7 2 K.DLMDLCDVVESK.F YJR064W 2 3 8.4% 562 61914 5.5 U CCT5 SGDID:S000003825, Chr X from 555828-557516, Verified ORF, "Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo" * 22_g_01_itms.25755.25755.3 4.5026 0.2766 99.7 3101.9944 3098.6108 11199.8 2 5.001 24.1 2 K.SQDDEIGDGTTGVVVLASALLDQALELIQK.G * 22_g_01_itms.07615.07615.2 2.5182 0.3176 100.0 2014.6921 2013.8711 6483.3 4 5.215 40.6 1 K.LEETCDDISASNDELFR.D Reverse_YDR190C 1 1 8.2% 463 50453 5.9 U RVB1 SGDID:S000002598, Chr IV from 841990-840599, reverse complement, Verified ORF, "Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family" * 22_g_01_itms.10646.10646.3 3.697 0.1761 99.2 3853.1343 3857.041 8577.6 200 4.743 18.2 1 V.EVSYLESGVLPCFPVKPGLEQSIALALATKGTSPGGAL.L Reverse_YLR449W 1 1 8.2% 392 43903 4.7 U FPR4 SGDID:S000004441, Chr XII from 1030829-1032007, Verified ORF, "Nuclear protein, putative peptidyl-prolyl cis-trans isomerase (PPIase) with similarity to Fpr3p; overproduction suppresses the growth defect resulting from the absence of E3 ubiquitin-protein ligase Tom1p" * 22_g_01_itms.27805.27805.3 3.6148 0.2718 98.8 3530.3943 3526.991 9925.0 1 4.539 21.8 1 D.FVKGNKLKGVYRMGVRTGKKAHPGKGTVRDEI.I YKL040C 2 4 8.2% 256 29174 5.0 U NFU1 SGDID:S000001523, Chr XI from 361471-360701, reverse complement, Verified ORF, "Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria" * 22_g_01_itms.20149.20149.3 4.0498 0.1994 99.7 2539.3145 2538.1987 8004.0 1 4.992 36.2 1 K.FELTEEDEEVSELIEELIDTR.I * 22_g_01_itms.20238.20238.2 5.4913 0.326 100.0 2542.7922 2538.1987 7784.5 1 6.653 60.0 3 K.FELTEEDEEVSELIEELIDTR.I Reverse_YOR136W 1 1 8.1% 369 39739 8.7 U IDH2 SGDID:S000005662, Chr XV from 580250-581359, Verified ORF, "Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle" * 22_g_01_itms.34929.34929.3 3.1602 0.0949 92.5 3378.8943 3380.7366 8059.6 307 3.917 19.8 1 T.YASPNTVVKLVSNDILETELTLDPYEKSLE.K Reverse_YHR187W 1 1 8.1% 309 35220 4.8 U IKI1 SGDID:S000001230, Chr VIII from 480990-481919, Verified ORF, "Subunit of the Elp4p-Iki1p-Elp6p-subcomplex of RNA polymerase II elongator complex, which is a histone acetyltransferase; iki1 mutations confer resistance to the K. lactis toxin zymocin" * 22_g_01_itms.24322.24322.2 2.0802 0.1792 91.4 2701.172 2704.5037 9166.7 85 4.332 22.9 1 E.LFPLAVQDKALKQKNSTGLNFTTLG.Q YLR172C 1 1 8.0% 300 33847 4.9 U DPH5 SGDID:S000004162, Chr XII from 502164-501262, reverse complement, Verified ORF, "Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm" * 22_g_01_itms.10807.10807.2 2.5267 0.217 95.5 2741.872 2738.1746 4845.6 6 4.49 30.4 1 K.DVANDQEYFKPAAWVPPTEDDSDE.- YDR533C 1 1 8.0% 237 25670 5.5 U HSP31 SGDID:S000002941, Chr IV from 1502150-1501437, reverse complement, Verified ORF, "Possible chaperone and cysteine protease with similarity to E. coli Hsp31 and S. cerevisiae Hsp32p, Hsp33p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer" * 22_g_01_itms.16346.16346.2 2.2768 0.2416 96.9 2001.9922 1998.0482 9131.9 15 4.747 33.3 1 G.VVAAVCHGPAIFDGLTDKK.T YKL054C 2 19 7.9% 738 83973 5.0 U DEF1 SGDID:S000001537, Chr XI from 338397-336181, reverse complement, Verified ORF, "RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII" * 22_g_01_itms.12914.12914.3 5.3712 0.3576 100.0 2839.8843 2837.221 8801.2 1 6.569 35.4 12 K.HDVPQDSNDNNNEELEAQGQQAQEK.N * 22_g_01_itms.14011.14011.3 4.6478 0.3005 100.0 3821.0645 3814.7517 9279.6 7 5.65 20.3 7 K.EQVKEEEQTAEELEQEQDNVAAPEEEVTVVEEK.V YMR186W 3 3 7.9% 705 80900 4.8 U HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock" * 22_g_01_itms.11763.11763.2 5.2191 0.3995 100.0 1998.2722 1997.9344 8489.5 1 7.93 71.9 1 K.LIEAFNEIAEDSEQFDK.F * 22_g_01_itms.13098.13098.3 3.0143 0.0857 92.9 4221.1143 4216.8867 7435.7 2 3.93 18.4 1 R.LISLGLNIDEDEETETAPEASTEAPVEEVPADTEMEEVD.- * 22_g_01_itms.09717.09717.3 3.9508 0.2686 100.0 3795.0544 3790.6023 7494.6 6 5.768 18.4 1 L.GLNIDEDEETETAPEASTEAPVEEVPADTEMEEVD.- YMR268C 1 1 7.9% 444 50849 9.3 U PRP24 SGDID:S000004881, Chr XIII from 804221-802887, reverse complement, Verified ORF, "Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling" * 22_g_01_itms.23680.23680.3 3.3015 0.1117 91.7 4017.7144 4023.897 5637.6 222 3.863 17.6 1 L.LDENLLRESFEGFGSIEKINIPAGQKEHSFNNCCA.F YNL330C 1 1 7.9% 433 48904 5.5 U RPD3 SGDID:S000005274, Chr XIV from 19302-18001, reverse complement, Verified ORF, "Histone deacetylase; regulates transcription and silencing" * 22_g_01_itms.21105.21105.3 3.1504 0.1386 98.7 3688.7043 3684.6304 8519.8 180 4.404 18.9 1 Q.PSAVVLQCGGDSLSGDRLGCFNLSMEGHANCVNY.V Reverse_YKR053C 1 1 7.9% 404 46488 7.7 U YSR3 SGDID:S000001761, Chr XI from 534923-533709, reverse complement, Verified ORF, "Dihydrosphingosine 1-phosphate phosphatase, membrane protein involved in sphingolipid metabolism; has similarity to Lcb3p" * 22_g_01_itms.25593.25593.3 3.3248 0.2139 91.6 3733.3743 3728.7034 7332.7 24 3.859 21.8 1 Y.RETLWDSCDLGSVVGIFAVSDEFCPCEDIPRV.H YDR385W 4 8 7.8% 842 93289 6.3 U EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" YOR133W 4 8 7.8% 842 93289 6.3 U EFT1 SGDID:S000005659, Chr XV from 575098-577626, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" 22_g_01_itms.06822.06822.2 5.2609 0.4309 100.0 1980.0122 1977.9227 6765.5 1 7.48 73.5 2 R.AEQLYEGPADDANCIAIK.N 22_g_01_itms.03073.03073.2 4.6191 0.4455 100.0 1496.4321 1495.7128 8574.5 1 7.692 76.9 2 R.ETVESESSQTALSK.S 22_g_01_itms.13862.13862.2 3.6411 0.107 96.4 2180.632 2179.1248 8641.6 14 4.676 42.1 1 K.AEPIDEEVSLAIENGIINPR.D 22_g_01_itms.08473.08473.2 4.5948 0.3859 100.0 1628.1322 1627.7063 8422.4 1 8.171 88.5 3 R.IMADDYGWDVTDAR.K YGR234W 1 1 7.8% 399 44646 6.3 U YHB1 SGDID:S000003466, Chr VII from 959908-961107, Verified ORF, "Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses" * 22_g_01_itms.25035.25035.3 3.8424 0.2562 98.3 3349.7644 3347.705 7721.6 14 4.337 23.3 1 K.EVLGDAATPEIINAWGEAYQAIADIFITVEK.K Reverse_YFR004W 1 1 7.8% 306 34398 6.2 U RPN11 SGDID:S000001900, Chr VI from 153388-154308, Verified ORF, "Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates" * 22_g_01_itms.32305.32305.3 3.3009 0.1784 97.4 2669.2744 2671.3625 8570.0 10 5.028 28.3 1 R.FADIVVKGKVS*QIPDVVVAVARS*N.L YPR108W 2 24 7.7% 429 48959 5.2 U RPN7 SGDID:S000006312, Chr XVI from 742452-743741, Verified ORF, "Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits" * 22_g_01_itms.17201.17201.3 5.5509 0.2528 100.0 3228.7744 3226.4705 8063.7 1 5.608 27.8 2 K.LEEDDEGELEQAQAWINLGEYYAQIGDK.D * 22_g_01_itms.18239.18239.3 6.0973 0.4024 100.0 3785.5745 3783.7148 9384.9 1 7.216 31.2 22 K.LEEDDEGELEQAQAWINLGEYYAQIGDKDNAEK.T Reverse_YPR023C 1 1 7.7% 401 45203 8.3 U EAF3 SGDID:S000006227, Chr XVI from 610028-608823, reverse complement, Verified ORF, "Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3" * 22_g_01_itms.35443.35443.3 2.4428 0.1791 94.1 3770.2144 3768.8916 10925.3 296 4.057 20.0 1 Q.LRELRYLLMNGLCKDFYLKLGACYESLQSQS.G YCR012W 2 2 7.7% 416 44738 7.6 U PGK1 SGDID:S000000605, Chr III from 137743-138993, Verified ORF, "3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis" * 22_g_01_itms.10790.10790.2 3.6051 0.1432 95.8 1963.3722 1962.9482 6465.8 46 4.564 44.1 1 K.DVTFLNDCVGPEVEAAVK.A * 22_g_01_itms.08489.08489.2 4.1591 0.3041 100.0 1579.7122 1579.7855 8532.8 1 7.181 69.2 1 K.VLENTEIGDSIFDK.A YKL193C 1 1 7.7% 338 38887 5.5 U SDS22 SGDID:S000001676, Chr XI from 79887-78871, reverse complement, Verified ORF, "Conserved nuclear regulatory subunit of Glc7p type 1 protein serine-threonine phosphatase (PP1), functions positively with Glc7p to promote dephosphorylation of nuclear substrates required for chromosome transmission during mitosis" * 22_g_01_itms.09539.09539.3 2.8881 0.1446 90.8 2937.5344 2940.4744 5358.8 130 3.835 23.0 1 T.DIWASFNKIDQSFESLGENLSALSRL.E Reverse_YMR081C 1 1 7.7% 338 37110 8.8 U ISF1 SGDID:S000004686, Chr XIII from 431094-430078, reverse complement, Verified ORF, "Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant" * 22_g_01_itms.14741.14741.3 3.109 0.2212 92.0 2821.2244 2822.296 8273.0 236 4.788 23.0 1 S.SAVSTNLSSNSPSKVLS*NSYSNNRTQ.L Reverse_YNL283C 1 1 7.6% 503 52292 7.0 U WSC2 SGDID:S000005227, Chr XIV from 106695-105184, reverse complement, Verified ORF, "Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response" * 22_g_01_itms.28907.28907.3 3.2959 0.1947 95.5 3842.9343 3837.876 9532.7 265 4.904 16.9 1 A.GGSLGKSKSS*SSKGNLLSSTSSSTSSVTAYVSTRTTFI.T YGR254W 1 1 7.6% 437 46816 6.6 U ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose" YHR174W 1 1 7.6% 437 46914 6.0 U ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" 22_g_01_itms.21007.21007.3 4.6397 0.3464 100.0 3258.2344 3257.6177 9187.7 1 6.03 26.6 1 R.YGASAGNVGDEGGVAPNIQTAEEALDLIVDAIK.A Reverse_YER039C 1 1 7.6% 249 27688 8.5 U HVG1 SGDID:S000000841, Chr V from 229204-228455, reverse complement, Verified ORF, "Protein of unknown function, has homology to Vrg4p" * 22_g_01_itms.08667.08667.3 2.9513 0.0863 93.0 2267.5745 2266.2197 6109.8 9 3.957 31.9 1 S.LNKTSWDEMIFSFVLLLPL.A YCL043C 4 16 7.5% 522 58227 4.5 U PDI1 SGDID:S000000548, Chr III from 50221-48653, reverse complement, Verified ORF, "Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds" * 22_g_01_itms.13482.13482.2 4.4146 0.4101 100.0 1940.8722 1940.913 6777.3 1 7.895 59.4 3 K.YGLPQLSEEAFDELSDK.I * 22_g_01_itms.10763.10763.3 3.8473 0.305 100.0 2342.5444 2341.9795 3970.1 1 6.461 39.3 2 K.AAEEADADAELADEEDAIHDEL.- * 22_g_01_itms.10733.10733.2 6.5992 0.4382 100.0 2346.5322 2341.9795 10473.9 1 8.499 64.3 9 K.AAEEADADAELADEEDAIHDEL.- * 22_g_01_itms.09912.09912.2 3.7012 0.3831 100.0 1871.2522 1870.7831 10174.2 1 6.325 53.1 2 A.DADAELADEEDAIHDEL.- YLR457C 2 4 7.5% 319 37354 10.2 U NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of the mitotic apparatus containing a coiled-coil domain, essential for the G2/M transition" * 22_g_01_itms.09291.09291.2 3.2457 0.2822 100.0 2805.1921 2804.2424 9728.9 1 6.231 37.0 2 R.DGNIPEMQPLQENISPACPT*PPYR.S * 22_g_01_itms.09335.09335.3 5.8758 0.3333 100.0 2806.8245 2804.2424 6794.4 2 5.994 40.2 2 R.DGNIPEMQPLQENISPACPT*PPYR.S YMR169C 1 1 7.3% 506 55385 5.7 U ALD3 SGDID:S000004779, Chr XIII from 600871-599351, reverse complement, Verified ORF, "Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose" YMR170C 1 1 7.3% 506 55188 5.6 U ALD2 SGDID:S000004780, Chr XIII from 603081-601561, reverse complement, Verified ORF, "Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p" 22_g_01_itms.25897.25897.3 3.09 0.1564 98.6 4054.5244 4053.0684 11366.4 2 4.464 20.1 1 I.FGPVVVVSKFTNYDDALKLANDTCYGLASAVFTKDVK.K YJL108C 1 1 7.3% 383 41488 8.0 U PRM10 SGDID:S000003644, Chr X from 218773-217622, reverse complement, Verified ORF, "Pheromone-regulated protein, predicted to have 5 transmembrane segments" * 22_g_01_itms.09421.09421.3 2.9954 0.2021 94.6 2833.1943 2829.4746 6199.7 351 4.084 22.2 1 I.FVQVPSGIASQNSLLSGLQSANTIVNAN.E YDL211C 1 1 7.3% 372 40752 5.5 U YDL211C SGDID:S000002370, Chr IV from 80413-79295, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.16160.16160.3 2.8996 0.0644 93.3 2788.5244 2785.6455 8612.8 56 3.986 23.1 1 A.NNRRTGVIVGSTVGVVIGVVIVIFIGF.I YPL262W 1 1 7.2% 488 53152 8.2 U FUM1 SGDID:S000006183, Chr XVI from 47336-48802, Verified ORF, "Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial localization determined by the N-terminal mitochondrial targeting sequence and protein conformation" * 22_g_01_itms.33657.33657.3 2.919 0.1615 95.1 3612.4443 3618.9746 11717.0 135 4.13 19.1 1 S.LKTLSFLAQGGTAVGTGLNTKPGFDVKIAEQISKE.T YNL178W 1 4 7.1% 240 26503 9.4 U RPS3 SGDID:S000005122, Chr XIV from 302682-303404, Verified ORF, "Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; has similarity to E. coli S3 and rat S3 ribosomal proteins" * 22_g_01_itms.05349.05349.2 3.8893 0.3271 100.0 1906.5922 1904.8514 4488.1 1 5.947 65.6 4 K.DYRPAEETEAQAEPVEA.- YFL010C 1 1 7.1% 211 22655 4.5 U WWM1 SGDID:S000001884, Chr VI from 115737-115102, reverse complement, Verified ORF, "WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes Gi phase growth arrest and clonal death that is suppressed by overexpression of MCA1" * 22_g_01_itms.32666.32666.3 2.8974 0.259 100.0 1560.9543 1563.6481 6650.1 16 5.476 39.3 1 Q.AYYGTAPSTSKGS*GH.G YHR075C 1 1 7.0% 400 44888 7.1 U PPE1 SGDID:S000001117, Chr VIII from 249643-248441, reverse complement, Verified ORF, "Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein" * 22_g_01_itms.37544.37544.3 2.4073 0.0677 96.3 3030.1743 3034.6829 7755.4 20 4.178 22.2 1 T.DLSHSFVGLPVSKLLILAGNENLDKELI.V YDL126C 3 24 6.9% 835 91996 4.9 U CDC48 SGDID:S000002284, Chr IV from 238664-236157, reverse complement, Verified ORF, "ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p and Ufd1p participates in retrotranslocation of ubiquitinated proteins from the ER into the cytosol for degradation by the proteasome" * 22_g_01_itms.26746.26746.3 4.0855 0.299 100.0 3341.9644 3340.5452 10227.2 6 5.344 25.9 5 R.EKMDLIDLDEDEIDAEVLDSLGVTMDNFR.F * 22_g_01_itms.20646.20646.3 4.2349 0.4142 100.0 3087.2043 3083.4077 11661.6 1 6.661 26.9 1 K.MDLIDLDEDEIDAEVLDSLGVTMDNFR.F * 22_g_01_itms.11349.11349.3 6.2885 0.3213 100.0 3209.5444 3204.4783 8770.1 1 6.681 35.7 18 K.VEGEDVEMTDEGAKAEQEPEVDPVPYITK.E YFL039C 2 6 6.9% 375 41690 5.7 U ACT1 SGDID:S000001855, Chr VI from 54695-54686,54377-53260, reverse complement, Verified ORF, "Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions" * 22_g_01_itms.01266.01266.2 2.276 0.305 100.0 977.03217 976.44885 5399.6 1 5.783 72.2 2 K.AGFAGDDAPR.A * 22_g_01_itms.10055.10055.2 4.0478 0.353 100.0 1792.2322 1790.8925 5703.2 1 6.046 73.3 4 K.SYELPDGQVITIGNER.F YBR121C 3 5 6.7% 667 75411 6.0 U GRS1 SGDID:S000000325, Chr II from 483361-481358, reverse complement, Verified ORF, "Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation" * 22_g_01_itms.09591.09591.2 3.9725 0.239 100.0 1638.3322 1637.7733 7981.2 1 6.807 67.9 2 K.IDGYSGPELGELMEK.Y * 22_g_01_itms.15551.15551.3 3.6382 0.1305 97.2 3275.9043 3272.4758 6131.3 87 4.228 22.4 1 R.DITYNGASWEEGTKDLTPFIAQAEAEAETD.- * 22_g_01_itms.12426.12426.2 3.8225 0.4939 100.0 1721.2122 1720.7917 8937.0 1 8.383 66.7 2 K.DLTPFIAQAEAEAETD.- YPL061W 1 4 6.6% 500 54414 5.4 U ALD6 SGDID:S000005982, Chr XVI from 432585-434087, Verified ORF, "Cytosolic aldehyde dehydrogenase that is activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for the conversion of acetaldehyde to acetate; constitutively expressed" * 22_g_01_itms.15660.15660.3 7.4162 0.4115 100.0 3744.5645 3737.5957 10249.7 1 7.933 33.6 4 K.TYPVEDPSTENTVCEVSSATTEDVEYAIECADR.A YAL009W 1 1 6.6% 259 30114 10.0 U SPO7 SGDID:S000000007, Chr I from 135856-136635, Verified ORF, "Integral nuclear/ER membrane protein of unknown function, required for normal nuclear envelope morphology and sporulation" * 22_g_01_itms.40101.40101.3 2.2104 0.1452 90.6 1769.2743 1766.8534 4592.8 434 3.815 32.8 1 V.GNHAQDDSASIVSGPRR.R Reverse_YBL055C 1 1 6.5% 418 47390 6.7 U YBL055C SGDID:S000000151, Chr II from 116832-115576, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.24427.24427.3 2.873 0.139 90.6 3222.7744 3216.7566 11242.8 46 3.818 22.1 1 A.HTRKIECWPADTELLLRETPIQKVVAL.N YAL025C 1 1 6.5% 306 35694 5.3 U MAK16 SGDID:S000000023, Chr I from 101146-100226, reverse complement, Verified ORF, "Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for normal concentration of free 60S ribosomal subunits; required for maintenance of M1 satellite double-stranded RNA of the L-A virus" * 22_g_01_itms.09881.09881.2 3.4813 0.2754 100.0 2402.5522 2402.0635 8801.6 6 5.447 39.5 1 K.VEIEYEEEHEVQNAEQEVAQ.- YDR463W 1 1 6.4% 519 58088 7.5 U STP1 SGDID:S000002871, Chr IV from 1386806-1388365, Verified ORF, "Transcription factor, activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing" * 22_g_01_itms.31774.31774.3 3.4073 0.2093 94.3 3781.1343 3786.0198 9405.3 25 4.064 19.5 1 E.NHIESGDCKALPQGYTKKNEKRSGKLRKIKTSN.G YBR181C 1 1 6.4% 236 26996 10.4 U RPS6B SGDID:S000000385, Chr II from 592769-592764,592411-591707, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein" YPL090C 1 1 6.4% 236 26996 10.4 U RPS6A SGDID:S000006011, Chr XVI from 378392-378387,377992-377288, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps6Bp and has similarity to rat S6 ribosomal protein" 22_g_01_itms.06038.06038.2 4.1298 0.401 100.0 1594.4922 1592.7444 8234.6 1 7.271 71.4 1 R.IGQEVDGEAVGDEFK.G YJL060W 1 1 6.3% 444 50082 6.6 U BNA3 SGDID:S000003596, Chr X from 323302-324636, Verified ORF, "Arylformamidase, involved in biosynthesis of nicotinic acid from tryptophan via kynurenine pathway; potential Cdc28p substrate" * 22_g_01_itms.15654.15654.3 3.9785 0.1688 97.4 3348.7144 3351.7893 7680.7 38 4.236 24.1 1 G.KDFRISHWLINELGVVAIPPTEFYIKEH.E YPL124W 3 9 6.3% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * 22_g_01_itms.08075.08075.2 3.8944 0.283 100.0 1922.6122 1922.8749 7545.8 120 6.02 43.3 6 R.SSQIHIENEST*EDILK.I * 22_g_01_itms.07410.07410.2 3.2723 0.1954 93.2 1924.4122 1922.8749 5718.0 4 5.149 50.0 1 R.SSQIHIENES*TEDILK.I * 22_g_01_itms.07781.07781.3 4.0728 0.2179 100.0 1924.5543 1922.8749 7085.9 1 5.407 43.3 2 R.SSQIHIENES*TEDILK.I YER036C 2 2 6.2% 610 68377 6.5 U YER036C SGDID:S000000838, Chr V from 225198-223366, reverse complement, Uncharacterized ORF, "ATPase of the ATP-binding cassette (ABC) family involved in ribosome biogenesis, has similarity to Gcn20p" * 22_g_01_itms.31467.31467.2 2.437 0.0824 92.0 2271.8323 2276.161 7485.0 1 4.363 42.1 1 N.YGRRYGLLGENGCGKSTFLK.A * 22_g_01_itms.12705.12705.2 2.6165 0.1159 91.4 2105.892 2103.0498 8500.0 43 4.329 35.3 1 K.TILEDGPESELLEPLYER.M Reverse_YGR152C 1 1 6.2% 272 30391 9.2 U RSR1 SGDID:S000003384, Chr VII from 795497-794679, reverse complement, Verified ORF, "GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases" * 22_g_01_itms.01790.01790.3 2.4278 0.1112 90.8 1679.9944 1681.8622 8522.9 55 3.835 35.9 1 A.SIGTRNSSGSKPVAHSP.K Reverse_YNL040W 1 1 6.1% 456 50964 6.5 U YNL040W SGDID:S000004985, Chr XIV from 553381-554751, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.27618.27618.3 2.8903 0.2103 90.5 3262.4644 3258.709 8876.2 362 3.806 20.4 1 E.EEMFYARKKGSAKLTNMLEESATRALRK.I YDR171W 2 4 6.1% 375 42817 5.1 U HSP42 SGDID:S000002578, Chr IV from 806616-807743, Verified ORF, "Small cytosolic stress-induced chaperone that forms barrel-shaped oligomers and suppresses the aggregation of non-native proteins; oligomer dissociation is not required for function; involved in cytoskeleton reorganization after heat shock" * 22_g_01_itms.11457.11457.2 3.5062 0.3946 100.0 2670.3523 2670.231 10392.4 1 6.969 38.6 2 R.IAIEEIPDEELEFEENPNPTVEN.- * 22_g_01_itms.11448.11448.3 3.2476 0.0754 90.6 2670.3843 2670.231 7434.1 82 3.81 27.3 2 R.IAIEEIPDEELEFEENPNPTVEN.- YKL104C 2 5 6.0% 717 80047 6.4 U GFA1 SGDID:S000001587, Chr XI from 245017-242864, reverse complement, Verified ORF, "Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis" * 22_g_01_itms.11708.11708.2 3.8579 0.2413 100.0 2376.5122 2372.1663 6752.7 1 5.321 52.5 3 K.VDFVDVEFPEENAGQPEIPLK.S * 22_g_01_itms.18834.18834.2 4.3589 0.3503 100.0 2470.0723 2465.245 5487.2 1 6.874 45.2 2 R.AIFEELSDIPVSVELASDFLDR.K YGL253W 1 2 6.0% 486 53943 5.3 U HXK2 SGDID:S000003222, Chr VII from 23935-25395, Verified ORF, "Hexokinase isoenzyme 2 that catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene" * 22_g_01_itms.15667.15667.3 5.7503 0.4047 100.0 3458.1543 3457.556 9418.8 4 6.633 25.9 2 R.IEEDPFENLEDTDDLFQNEFGINTTVQER.K YMR314W 1 1 6.0% 234 25604 7.4 U PRE5 SGDID:S000004931, Chr XIII from 901708-902412, Verified ORF, "20S proteasome alpha-type subunit" * 22_g_01_itms.08091.08091.2 2.8586 0.2438 99.3 1527.4521 1526.7379 7635.0 3 4.97 53.8 1 K.DTPFTIYDGEAVAK.Y YGR187C 1 2 5.8% 394 44952 4.7 U HGH1 SGDID:S000003419, Chr VII from 871421-870237, reverse complement, Verified ORF, "Protein of unknown function with similarity to human HMG1 and HMG2; localizes to the cytoplasm" * 22_g_01_itms.15343.15343.2 3.8552 0.3964 100.0 2734.7322 2734.2292 7532.0 1 7.169 38.6 2 K.DSEIDEEDMFNLPDELQLLPEDK.E Reverse_YOL077C 1 1 5.8% 291 33577 9.2 U BRX1 SGDID:S000005437, Chr XV from 186722-185847, reverse complement, Verified ORF, "Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif" * 22_g_01_itms.10100.10100.3 2.7104 0.1409 92.1 1963.8544 1964.9791 7158.4 163 3.912 32.8 1 K.DDVISFSMVHDIFPKSK.R Reverse_YDL168W 1 1 5.7% 386 41042 6.8 U SFA1 SGDID:S000002327, Chr IV from 159605-160765, Verified ORF, "Bifunctional enzyme containing both alcohol dehydrogenase and glutathione-dependent formaldehyde dehydrogenase activities, functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p" * 22_g_01_itms.12251.12251.2 2.2251 0.2547 96.2 2352.8523 2348.3125 8020.9 17 4.656 31.0 1 E.VKLAGKQYDKILGGMESRGKIG.G YPL131W 1 4 5.7% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * 22_g_01_itms.14373.14373.2 4.2256 0.3952 100.0 2094.372 2092.9868 6760.8 1 6.898 62.5 4 R.FPGWDFETEEIDPELLR.S YCR002C 1 1 5.6% 322 37025 5.7 U CDC10 SGDID:S000000595, Chr III from 118345-117377, reverse complement, Verified ORF, "Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM" * 22_g_01_itms.10191.10191.2 2.7442 0.2605 97.0 2228.3323 2228.0247 5794.7 1 4.725 47.1 1 K.IYPYDSEELTDEELELNR.S YJR109C 5 9 5.5% 1118 123915 5.3 U CPA2 SGDID:S000003870, Chr X from 632856-629500, reverse complement, Verified ORF, "Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor" * 22_g_01_itms.18276.18276.3 3.5313 0.1327 90.6 3995.0942 3995.9185 8200.9 1 3.816 20.0 1 T.SEDRDLFASALKDINIPIAESFACETVDEALEAAER.V * 22_g_01_itms.18275.18275.3 4.9327 0.4302 100.0 2665.0745 2663.251 6101.2 1 7.631 41.3 2 K.DINIPIAESFACETVDEALEAAER.V * 22_g_01_itms.18302.18302.2 6.0092 0.4217 100.0 2668.3123 2663.251 7407.8 1 8.357 58.7 3 K.DINIPIAESFACETVDEALEAAER.V * 22_g_01_itms.14820.14820.2 4.8764 0.4239 100.0 2824.7122 2824.3416 7047.7 1 7.252 43.8 2 K.FSSILDSIDVDQPEWSELTSVEEAK.L * 22_g_01_itms.15134.15134.2 2.9909 0.1903 95.5 2464.7922 2462.1462 10239.1 58 4.478 31.0 1 S.SILDSIDVDQPEWSELTSVEEA.K YOL023W 1 1 5.5% 676 75657 8.7 U IFM1 SGDID:S000005383, Chr XV from 278056-280086, Verified ORF, "Mitochondrial translation initiation factor 2" * 22_g_01_itms.28474.28474.3 2.8974 0.1541 90.2 3748.3145 3753.7249 7124.7 5 3.798 20.1 1 C.DVSGSAEAVSESISSLGNDEVRCNVISSSVGIPTESD.L Reverse_YIL037C 1 1 5.5% 656 75021 8.0 U PRM2 SGDID:S000001299, Chr IX from 284998-283028, reverse complement, Verified ORF, "Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p" * 22_g_01_itms.23095.23095.3 2.9387 0.2037 96.4 3718.5544 3715.8635 8025.9 1 4.204 21.4 1 T.TTATTTATATATSISAFTSVTDNRKVQAHSMKAMTI.D YBR015C 1 1 5.5% 597 67774 6.3 U MNN2 SGDID:S000000219, Chr II from 269503-267710, reverse complement, Verified ORF, "Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment" * 22_g_01_itms.23665.23665.3 3.4222 0.2459 98.6 3707.1843 3706.6895 9490.8 16 5.061 20.3 1 Y.NVNGPTWYYPIFSQKAAGEGDKET*FIAAANFYG.L YER021W 1 1 5.5% 523 60393 5.6 U RPN3 SGDID:S000000823, Chr V from 196947-198518, Verified ORF, "Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control" * 22_g_01_itms.23083.23083.3 3.9476 0.0982 92.0 3467.9343 3466.5925 8349.3 19 3.896 23.2 1 K.INHEDGFIETTELLNIYDSEDPQQVFDER.I Reverse_YIL135C 1 1 5.5% 436 47961 9.2 U VHS2 SGDID:S000001397, Chr IX from 96375-95065, reverse complement, Verified ORF, "Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p" * 22_g_01_itms.20330.20330.2 2.1634 0.1888 98.7 2601.9722 2605.2468 8854.4 61 4.915 28.3 1 S.SNNHNFLSTRSSQRSLSNAANNSV.T YOR373W 2 2 5.4% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * 22_g_01_itms.13056.13056.3 4.2872 0.1045 98.6 2943.9844 2941.182 4199.4 1 5.039 35.6 1 K.VSSSFS*DDS*DSGPAAEAHDVFDGILQK.Q * 22_g_01_itms.08298.08298.2 3.7856 0.4319 100.0 2302.1921 2301.9417 8150.0 14 6.557 38.9 1 R.VEDITSIS*EVNTS*FNETEK.Q YHL027W 1 1 5.4% 625 68232 7.2 U RIM101 SGDID:S000001019, Chr VIII from 51109-52986, Verified ORF, "Transcriptional repressor involved in the response to pH; required for alkaline pH-stimulated differentiation pathways such as haploid invasive growth and sporulation; activated by proteolytic processing; has similarity to the A. nidulans transcription factor PacC" * 22_g_01_itms.21016.21016.3 3.3646 0.0744 91.7 3559.6143 3562.7737 8008.7 366 3.861 18.2 1 N.KENGTAAPQHSRESIVENGTDVSNVTKKDGLPSP.N Reverse_YDL066W 1 1 5.4% 428 48190 8.8 U IDP1 SGDID:S000002224, Chr IV from 334835-336121, Verified ORF, "Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes" * 22_g_01_itms.00651.00651.3 2.2412 0.0158 92.8 2446.4944 2445.176 7522.5 120 3.928 25.0 1 G.FGQAVIDSQVDGDYNKLAMIFGG.K Reverse_YDR528W 1 1 5.4% 423 47578 6.7 U HLR1 SGDID:S000002936, Chr IV from 1494576-1495847, Verified ORF, "Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A" * 22_g_01_itms.29605.29605.3 3.2972 0.0916 97.5 2601.9543 2600.3643 9387.0 61 4.275 25.0 1 K.KISSSQPSLRVRENISTQLNDID.Q YLR449W 1 1 5.4% 392 43903 4.7 U FPR4 SGDID:S000004441, Chr XII from 1030829-1032007, Verified ORF, "Nuclear protein, putative peptidyl-prolyl cis-trans isomerase (PPIase) with similarity to Fpr3p; overproduction suppresses the growth defect resulting from the absence of E3 ubiquitin-protein ligase Tom1p" * 22_g_01_itms.27308.27308.2 2.3906 0.2139 92.0 2613.5923 2609.485 7817.9 2 4.364 35.0 1 E.LVKKDEKKKNNKKDSKRKHEE.D YGR023W 1 1 5.3% 551 57528 4.7 U MTL1 SGDID:S000003255, Chr VII from 529268-530923, Verified ORF, "Protein with both structural and functional similarity to Mid2p, which is a plasma membrane sensor required for cell integrity signaling during pheromone-induced morphogenesis; suppresses rgd1 null mutations" * 22_g_01_itms.25154.25154.3 3.0809 0.1436 92.8 2829.5942 2830.227 9030.4 3 3.951 25.0 1 L.SSTLSSELSSSSMQVSSSSTSSSSSEVTS.S YKL189W 1 1 5.3% 399 45853 8.4 U HYM1 SGDID:S000001672, Chr XI from 84709-85908, Verified ORF, "Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response" * 22_g_01_itms.00716.00716.3 2.8107 0.1174 98.6 2328.1743 2326.317 7492.7 386 4.485 25.0 1 Y.LVSQPKTISLMLRTAEVALQQ.K YDR432W 2 3 5.3% 414 45407 5.5 U NPL3 SGDID:S000002840, Chr IV from 1328773-1330017, Verified ORF, "RNA-binding protein that carries poly(A)+ mRNA from the nucleus into the cytoplasm; phosphorylation by Sky1p in the cytoplasm may promote release of mRNAs" * 22_g_01_itms.17136.17136.2 4.6026 0.3217 100.0 2438.4321 2437.1775 6422.6 1 5.88 45.2 2 R.DFDGTGALEFPSEEILVEALER.L * 22_g_01_itms.17156.17156.3 2.9535 0.2002 97.9 2438.6042 2437.1775 7242.0 114 4.286 26.2 1 R.DFDGTGALEFPSEEILVEALER.L YPL113C 1 1 5.3% 396 45014 7.2 U YPL113C SGDID:S000006034, Chr XVI from 337142-335952, reverse complement, Uncharacterized ORF, "Putative dehydrogenase" * 22_g_01_itms.29439.29439.2 2.6155 0.2362 95.8 2228.9521 2228.2192 10363.9 11 4.561 35.0 1 V.LILGFGSIGQNIGSNLHKVFN.M Reverse_YOL047C 1 1 5.3% 356 40878 8.6 U YOL047C SGDID:S000005407, Chr XV from 242745-242503,242439-241612, reverse complement, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * 22_g_01_itms.11201.11201.3 3.2924 0.0586 95.8 2149.4043 2146.235 4981.4 29 4.158 36.1 1 I.CVLLSWALISTFAVILLLN.L Reverse_YKL005C 1 1 5.2% 594 67901 5.4 U BYE1 SGDID:S000001488, Chr XI from 434520-432736, reverse complement, Verified ORF, "Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit" * 22_g_01_itms.10541.10541.3 3.0893 0.0066 95.0 3613.8843 3610.8884 5991.8 83 4.114 19.2 1 E.KFIDRRLKQSAGIYNLYGTFELGLGPYLFAC.N YDR190C 1 2 5.2% 463 50453 5.9 U RVB1 SGDID:S000002598, Chr IV from 841990-840599, reverse complement, Verified ORF, "Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family" * 22_g_01_itms.09662.09662.2 5.3441 0.3273 100.0 2598.2722 2596.1943 5984.9 1 6.536 56.5 2 K.EVYEGEVTELTPEDAENPLGGYGK.T Reverse_YBR057C 1 1 5.2% 366 41433 5.9 U MUM2 SGDID:S000000261, Chr II from 353291-352191, reverse complement, Verified ORF, "Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex" * 22_g_01_itms.19219.19219.3 4.3784 0.1191 90.9 2212.1343 2212.9385 9216.5 1 3.837 41.7 1 E.MRDDKNSNMENSNVNNMSL.A YDR158W 1 1 5.2% 365 39544 6.7 U HOM2 SGDID:S000002565, Chr IV from 770352-771449, Verified ORF, "Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis" * 22_g_01_itms.11186.11186.2 2.9475 0.3025 97.8 2075.7522 2072.9487 9579.1 6 4.812 36.1 1 K.ECDIVFSGLDADYAGAIEK.E Reverse_YOL011W 1 1 5.1% 686 75077 5.0 U PLB3 SGDID:S000005371, Chr XV from 305349-307409, Verified ORF, "Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro" * 22_g_01_itms.25056.25056.3 2.9681 0.1054 91.6 3865.3145 3871.9492 7057.5 11 3.87 19.9 1 N.DVAFIIDVDREKQILPLVPVNEDDEGGDVLFLSDS.D YKL103C 1 1 5.1% 514 57093 5.8 U LAP4 SGDID:S000001586, Chr XI from 247326-245782, reverse complement, Verified ORF, "Vacuolar aminopeptidase, often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway" * 22_g_01_itms.15294.15294.3 3.703 0.1347 93.3 2948.7244 2947.3696 8507.0 51 4.003 25.0 1 K.DVNTEESDLFSTVTLYDNEEIGSLTR.Q Reverse_YBR197C 1 1 5.1% 217 24203 9.1 U YBR197C SGDID:S000000401, Chr II from 615851-615198, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene" * 22_g_01_itms.08965.08965.2 1.9818 0.2001 94.5 1221.3121 1222.6028 6953.3 8 4.461 65.0 1 S.DSSGKRKDKDS.K YBL055C 1 1 5.0% 418 47390 6.7 U YBL055C SGDID:S000000151, Chr II from 116832-115576, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.13689.13689.2 2.3438 0.2534 97.1 2429.9521 2434.286 8767.5 1 5.484 37.5 1 P.DRKLVVHSFTGS*AIDLQKLLN.L YNL037C 1 2 5.0% 360 39324 9.0 U IDH1 SGDID:S000004982, Chr XIV from 559003-557921, reverse complement, Verified ORF, "Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle" * 22_g_01_itms.07922.07922.2 3.6197 0.2213 99.3 1901.6322 1898.8983 6893.7 12 5.021 44.1 2 R.DIGGSSSTTDFTNEIINK.L YMR300C 2 4 4.9% 510 56719 6.3 U ADE4 SGDID:S000004915, Chr XIII from 867090-865558, reverse complement, Verified ORF, "Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway" * 22_g_01_itms.14315.14315.2 3.8171 0.4223 100.0 2838.8123 2838.2998 7724.1 1 6.994 39.6 2 K.FEDGVFTGNYVTGVEDGYIQELEEK.R * 22_g_01_itms.14294.14294.3 5.7269 0.2582 100.0 2840.3342 2838.2998 9921.8 1 6.849 35.4 2 K.FEDGVFTGNYVTGVEDGYIQELEEK.R YML127W 1 1 4.8% 581 65218 7.8 U RSC9 SGDID:S000004596, Chr XIII from 17065-18810, Verified ORF, "One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway" * 22_g_01_itms.28794.28794.3 3.2219 0.1594 93.4 3208.3743 3204.7117 9354.2 2 3.979 25.0 1 N.LDHKTLSLIVSFLLLECDSELIIASLDF.L Reverse_YML123C 1 1 4.8% 587 64382 6.4 U PHO84 SGDID:S000004592, Chr XIII from 25801-24038, reverse complement, Verified ORF, "High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p" * 22_g_01_itms.25770.25770.3 3.2028 0.1797 95.4 3168.0544 3164.7375 8783.8 398 4.141 22.2 1 I.VYLALLGHDGLKHYAFGIVCFLATLIIF.G YGL206C 3 4 4.7% 1653 187233 5.2 U CHC1 SGDID:S000003174, Chr VII from 107508-102547, reverse complement, Verified ORF, "Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; two heavy chains form the clathrin triskelion structural component; the light chain (CLC1) is thought to regulate function" * 22_g_01_itms.11803.11803.2 2.9457 0.1978 100.0 1872.7522 1869.938 8620.5 2 5.533 50.0 1 K.DANLEDQLPLVIVCDR.F * 22_g_01_itms.25213.25213.3 3.2205 0.1978 93.3 4377.8345 4374.21 10150.2 2 3.991 17.3 1 K.TAQVVGALLDMDCDEAFIQSLLQSVLGQVPINELTTEVEK.R * 22_g_01_itms.24033.24033.2 3.5569 0.2051 93.3 2538.5923 2536.2249 9278.8 1 4.417 42.9 2 R.YESNGYFEELISLFEAGLGLER.A YCR088W 1 1 4.6% 592 65576 4.7 U ABP1 SGDID:S000000684, Chr III from 265064-266842, Verified ORF, "Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization" * 22_g_01_itms.10464.10464.3 3.6288 0.2194 98.1 3037.5842 3035.3606 6557.4 335 4.304 23.1 1 K.QEEAPEQAPEEEIEEEAEEAAPQLPSR.S YMR019W 1 1 4.5% 949 109825 7.3 U STB4 SGDID:S000004621, Chr XIII from 312155-315004, Verified ORF, "Protein that binds Sin3p in a two-hybrid assay; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins" * 22_g_01_itms.27466.27466.3 2.6868 0.089 92.0 4321.0444 4329.1484 9592.0 69 3.887 16.7 1 L.ATRSHNTTNFDGSIAISPESAVANLGTDTGLPSDVLDTVSKIG.N YJL083W 1 1 4.5% 604 68768 9.6 U TAX4 SGDID:S000003619, Chr X from 278757-280571, Verified ORF, "Protein involved in regulation of phosphatidylinositol 4,5-bisphosphate concentrations; Irs4p and Tax4p bind and activate the phosphatase Inp51p" * 22_g_01_itms.11085.11085.3 3.2529 0.213 92.2 2847.3843 2845.387 6615.9 103 3.91 25.0 1 I.HYDVRNKASNSPSSIAAAETAAYLAHT.N Reverse_YGR023W 1 1 4.5% 551 57528 4.7 U MTL1 SGDID:S000003255, Chr VII from 529268-530923, Verified ORF, "Protein with both structural and functional similarity to Mid2p, which is a plasma membrane sensor required for cell integrity signaling during pheromone-induced morphogenesis; suppresses rgd1 null mutations" * 22_g_01_itms.39982.39982.3 2.5565 0.1193 94.4 2646.0544 2644.228 6594.9 3 4.078 25.0 1 S.LGSHHSNKGSSASATPSYTITTYYN.S Reverse_YBR042C 1 1 4.5% 397 45515 9.3 U YBR042C SGDID:S000000246, Chr II from 321609-320416, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.27813.27813.2 2.27 0.2887 99.0 1715.5922 1716.8881 7440.7 1 4.937 44.1 1 S.GAGRANSDLGALSNSLTI.K YFL018C 1 1 4.4% 499 54010 8.0 U LPD1 SGDID:S000001876, Chr VI from 103121-101622, reverse complement, Verified ORF, "Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes" * 22_g_01_itms.11569.11569.2 3.7526 0.3138 100.0 2361.9521 2358.208 8445.6 9 5.438 35.7 1 K.NIIVATGSEVTPFPGIEIDEEK.I YLL057C 1 1 4.4% 412 46983 6.7 U JLP1 SGDID:S000003980, Chr XII from 26994-25756, reverse complement, Uncharacterized ORF, "Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)" * 22_g_01_itms.01250.01250.3 2.54 0.0417 95.1 1831.3143 1829.8234 8226.0 12 4.125 33.8 1 T.FFSVVEGPDGGGDTLFAD.T YER147C 1 1 4.3% 624 72141 7.8 U SCC4 SGDID:S000000949, Chr V from 464837-462963, reverse complement, Verified ORF, "Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX" * 22_g_01_itms.20434.20434.3 3.9736 0.1706 99.2 2817.6843 2814.3909 9138.7 329 4.704 25.0 1 M.KVQFEGGGAVEEYTRLAQSGGTSSEVK.M YDR143C 1 1 4.3% 610 65618 5.2 U SAN1 SGDID:S000002550, Chr IV from 743869-742037, reverse complement, Verified ORF, "Ubiquitin-protein ligase, involved in the proteasome-dependent degradation of aberrant nuclear proteins; san1 mutations suppress sir4, spt16, and cdc68 mutations, suggesting a role in chromatin silencing" * 22_g_01_itms.19933.19933.3 3.2149 0.2821 98.5 2909.8442 2909.262 9182.9 54 5.158 25.0 1 S.ST*TQNPPSNSGGS*NNNQSPRWVPIPL.T YER165W 1 2 4.3% 577 64344 6.0 U PAB1 SGDID:S000000967, Chr V from 510368-512101, Verified ORF, "Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G" * 22_g_01_itms.15095.15095.3 3.807 0.2675 100.0 2787.5645 2786.2896 8244.5 1 5.349 32.3 2 K.NLDDSVDDEKLEEEFAPYGTITSAK.V YLR286C 1 1 4.3% 562 59015 4.5 U CTS1 SGDID:S000004276, Chr XII from 710138-708450, reverse complement, Verified ORF, "Endochitinase, required for cell separation after mitosis; transcriptional activation during late G and early M cell cycle phases is mediated by transcription factor Ace2p" * 22_g_01_itms.34756.34756.2 2.5778 0.1236 93.0 2487.372 2486.1257 10031.8 2 5.156 30.4 1 K.NIKLFLGLPGSASAAGSGYISDT*S*.L YDL044C 1 1 4.3% 440 51192 6.7 U MTF2 SGDID:S000002202, Chr IV from 375286-373964, reverse complement, Verified ORF, "Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription" * 22_g_01_itms.10761.10761.2 2.7189 0.1699 90.7 2222.4321 2225.2043 10015.9 286 4.32 30.6 1 A.MTLPYIIVKSLRLGDFDFP.A Reverse_YOR250C 1 1 4.3% 445 50226 5.9 U CLP1 SGDID:S000005776, Chr XV from 802307-800970, reverse complement, Verified ORF, "Subunit of cleavage factor I (CFI), involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation" * 22_g_01_itms.07224.07224.3 3.4225 0.0371 90.4 2129.3643 2125.2021 8616.5 215 3.806 29.2 1 L.KGEAKLDIQWDSGKPIVLK.H YNL021W 1 1 4.2% 706 80070 5.5 U HDA1 SGDID:S000004966, Chr XIV from 593228-595348, Verified ORF, "Putative catalytic subunit of a class II histone deacetylase complex that also contains Hda2p and Hda3p; Hda1p interacts with the Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation" * 22_g_01_itms.14059.14059.3 3.7833 0.1926 90.6 3314.6643 3310.8625 7068.1 1 3.816 25.9 1 R.DTRAVTKTVINFLGDKQLKPLVPLVDETLS.E YNL016W 1 1 4.2% 453 50763 5.1 U PUB1 SGDID:S000004961, Chr XIV from 602908-604269, Verified ORF, "Poly(A)+ RNA-binding protein, abundant mRNP-component protein hypothesized to bind a pool of non-translatable mRNAs; not reported to associate with polyribosomes" * 22_g_01_itms.12950.12950.3 3.0373 0.2502 98.7 2256.0842 2259.1023 7706.8 17 5.03 31.9 1 G.RET*SDRVLYVGNLDKAITE.D Reverse_YER154W 1 2 4.2% 402 44816 10.1 U OXA1 SGDID:S000000956, Chr V from 475015-476223, Verified ORF, "Translocase of the mitochondrial inner membrane, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; null is respiratory deficient" * 22_g_01_itms.38710.38710.3 2.506 0.1493 97.3 1867.2244 1865.9722 5077.1 275 4.27 32.8 2 N.LADLEPKIHSNRAVTDS.S YML119W 1 1 4.2% 357 40060 9.6 U YML119W SGDID:S000004588, Chr XIII from 30611-31684, Uncharacterized ORF, "Protein of unknown function, potential Cdc28p substrate" * 22_g_01_itms.01569.01569.3 2.9122 0.1797 98.6 1834.1044 1835.9033 9381.1 50 5.056 39.3 1 P.IQLSTNSKTRIS*KS*L.D YJL213W 1 1 4.2% 331 35905 7.0 U YJL213W SGDID:S000003749, Chr X from 32163-33158, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.21219.21219.3 2.3793 0.1308 96.5 1379.8744 1377.7313 6177.9 11 4.186 40.4 1 R.RGADFIKIMGGGGV.A Reverse_YGL205W 1 1 4.1% 748 84042 8.5 U POX1 SGDID:S000003173, Chr VII from 108162-110408, Verified ORF, "Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix" * 22_g_01_itms.23429.23429.3 3.8078 0.1077 92.1 3667.1343 3670.056 8843.4 407 3.902 19.2 1 I.CDKRVIPLLALLQPQVVKSIQDPTFIKFQQF.E YMR001C 1 1 4.1% 705 81031 9.0 U CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate" * 22_g_01_itms.24906.24906.3 3.9325 0.1729 92.6 3257.1243 3261.782 8200.1 3 3.922 25.0 1 L.NTRSKLVHTPIKGNTADLVGKENHFKQTK.R YBR286W 1 1 4.1% 537 60138 5.3 U APE3 SGDID:S000000490, Chr II from 774696-776309, Verified ORF, "Vacuolar aminopeptidase Y, processed to mature form by Prb1p" * 22_g_01_itms.19284.19284.2 2.4274 0.2722 99.3 2408.9922 2412.1836 7404.5 1 5.0 35.7 1 P.QLNDDDSSAVAANIPKPHIPYF.M YBR273C 1 1 4.1% 436 50031 6.3 U UBX7 SGDID:S000000477, Chr II from 749366-748056, reverse complement, Uncharacterized ORF, "UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p" * 22_g_01_itms.46042.46042.3 2.1798 0.1271 93.5 1840.0443 1842.9058 9960.8 43 4.018 30.9 1 P.NRHEVRSSTPLSGAASSS.C YOR187W 1 3 4.1% 437 47972 6.7 U TUF1 SGDID:S000005713, Chr XV from 684030-685343, Verified ORF, "Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans" * 22_g_01_itms.14730.14730.2 4.2964 0.4215 100.0 2168.872 2164.9817 9966.3 1 7.382 55.9 3 K.VDTIDDPEMLELVEMEMR.E YBL050W 1 1 4.1% 292 32803 5.1 U SEC17 SGDID:S000000146, Chr II from 125128-125157,125274-126122, Verified ORF, "Peripheral membrane protein required for vesicular transport between ER and Golgi and for the 'priming' step in homotypic vacuole fusion, part of the cis-SNARE complex; has similarity to alpha-SNAP" * 22_g_01_itms.21598.21598.3 2.0479 0.1696 96.5 1202.2144 1201.6177 3456.8 320 4.184 36.4 1 T.DAVAAARTLQEG.Q YDR060W 2 4 4.0% 1025 116676 5.0 U MAK21 SGDID:S000002467, Chr IV from 570645-573722, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein" * 22_g_01_itms.14084.14084.2 5.3846 0.3625 100.0 2498.392 2494.996 6959.7 1 6.519 57.5 3 K.ASNFDS*DDEMDENEIWSALVK.S * 22_g_01_itms.13935.13935.2 4.0852 0.3457 100.0 2300.892 2299.9285 10077.6 1 6.96 50.0 1 K.SLPVFASADDYAQYLDQDS*D.- YGL137W 1 3 4.0% 889 99445 4.7 U SEC27 SGDID:S000003105, Chr VII from 249874-249891,250092-252743, Verified ORF, "Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP" * 22_g_01_itms.21694.21694.3 5.6906 0.3746 100.0 3857.9644 3850.7307 9406.3 1 7.086 25.0 3 K.DAYLEAANNGNIDDSEGVDEAFDVLYELSESITSGK.W YJR045C 2 7 4.0% 654 70628 5.6 U SSC1 SGDID:S000003806, Chr X from 521516-519552, reverse complement, Verified ORF, "Mitochondrial matrix ATPase that is a subunit of the presequence translocase-associated protein import motor (PAM); involved in protein translocation into the matrix and protein folding; member of the heat shock protein 70 (HSP70) family" * 22_g_01_itms.12709.12709.3 5.3871 0.3354 100.0 2713.2844 2710.2366 7810.2 1 6.657 34.0 3 K.DSSITVAGSSGLSENEIEQMVNDAEK.F * 22_g_01_itms.12804.12804.2 4.9431 0.3926 100.0 2714.912 2710.2366 10408.5 1 7.459 40.0 4 K.DSSITVAGSSGLSENEIEQMVNDAEK.F YHR065C 1 1 4.0% 501 55969 9.2 U RRP3 SGDID:S000001107, Chr VIII from 229039-227534, reverse complement, Verified ORF, "Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity" * 22_g_01_itms.10572.10572.3 3.0911 0.0694 98.7 2343.8943 2342.259 5030.4 6 4.413 28.9 1 L.MRKPHIIIATPGRLMDHLEN.T Reverse_YMR030W 1 1 4.0% 376 43460 8.2 U RSF1 SGDID:S000004632, Chr XIII from 330792-331922, Verified ORF, "Protein required for respiratory growth; localized to both the nucleus and mitochondrion; mutant displays decreased transcription of specific nuclear and mitochondrial genes whose products are involved in respiratory growth" * 22_g_01_itms.02522.02522.3 2.7284 0.253 99.4 1523.8744 1526.72 7955.2 50 4.827 37.5 1 L.SSGTEPHNGNVDGKK.K YIL075C 1 3 3.9% 945 104232 6.2 U RPN2 SGDID:S000001337, Chr IX from 220697-217860, reverse complement, Verified ORF, "Subunit of the 26S proteasome, substrate of the N-acetyltransferase Nat1p" * 22_g_01_itms.25731.25731.3 4.4666 0.2632 100.0 4291.7344 4289.989 9602.8 1 5.803 23.6 3 K.TYALESINNVVDQLWSEISNELPDIEALYDDDTFSDR.E YMR016C 1 1 3.9% 785 85643 8.5 U SOK2 SGDID:S000004618, Chr XIII from 305592-303235, reverse complement, Verified ORF, "Nuclear protein that plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; negatively regulates pseudohyphal differentiation; homologous to several transcription factors" * 22_g_01_itms.22252.22252.3 3.4382 0.1487 95.1 3362.1243 3363.5405 7722.7 22 4.13 20.8 1 A.YYYYPANGDGTTNGATPSVTSNQVQNPNLEK.T Reverse_YFL047W 1 1 3.9% 714 82209 5.8 U RGD2 SGDID:S000001847, Chr VI from 40421-42565, Verified ORF, "GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p" * 22_g_01_itms.26904.26904.3 3.3818 0.219 93.3 3259.9443 3263.5862 9863.6 68 3.966 20.4 1 I.KNGLTSCFDLTAKKIANLRDSECKEMFS.L YHR020W 2 6 3.9% 688 77386 6.4 U YHR020W SGDID:S000001062, Chr VIII from 143989-146055, Uncharacterized ORF, "Protein required for cell viability" * 22_g_01_itms.25572.25572.2 4.9397 0.493 100.0 3053.672 3051.5818 9051.6 1 9.096 42.3 2 K.DAEEEVLQILDFYAGVYEELLAVPVVK.G * 22_g_01_itms.25644.25644.3 5.7014 0.3734 100.0 3056.9343 3051.5818 8862.4 1 6.822 30.8 4 K.DAEEEVLQILDFYAGVYEELLAVPVVK.G YDL097C 1 2 3.9% 434 49774 6.3 U RPN6 SGDID:S000002255, Chr IV from 286695-285391, reverse complement, Verified ORF, "Essential, non-ATPase regulatory subunit of the 26S proteasome lid required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion" * 22_g_01_itms.10196.10196.2 3.4548 0.2045 100.0 2072.7722 2068.9539 5679.1 2 5.422 50.0 2 K.FEQVPDSLDDQIFVCEK.S Reverse_YHR027C 2 3 3.8% 993 109492 4.6 U RPN1 SGDID:S000001069, Chr VIII from 164704-161723, reverse complement, Verified ORF, "Non-ATPase base subunit of the 19S regulatory particle of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein?protein interactions" * 22_g_01_itms.00816.00816.2 2.9281 0.1538 98.1 1224.6721 1222.4785 9127.6 1 4.817 75.0 2 E.GEADVEMEDVE.I * 22_g_01_itms.26884.26884.3 3.288 0.0611 91.8 3096.7444 3099.5679 11593.2 91 3.876 24.0 1 N.LEKALYLFHESLKGNGIIDQVGEYEFS.T Reverse_YOR113W 1 2 3.8% 914 101170 7.4 U AZF1 SGDID:S000005639, Chr XV from 534075-536819, Verified ORF, "Zinc-finger transcription factor, involved in induction of CLN3 transcription in response to glucose; genetic and physical interactions indicate a possible role in mitochondrial transcription or genome maintenance" * 22_g_01_itms.27585.27585.3 3.2435 0.0686 90.7 3826.2244 3824.0208 11416.1 41 3.82 18.4 2 P.HNEPSSITSKKTGVGKGRGKIGRNSNKYISAFYEL.L Reverse_YNL068C 1 1 3.8% 862 94374 9.3 U FKH2 SGDID:S000005012, Chr XIV from 498290-495702, reverse complement, Verified ORF, "Transcription factor of the forkhead family that regulates the cell cycle and pseudohyphal growth; also involved in chromatin silencing at HML and HMR; potential Cdc28p substrate" * 22_g_01_itms.23723.23723.3 3.4936 0.264 93.0 3623.2444 3621.7566 7765.3 200 4.789 18.8 1 F.AKTPSRLLTDYPQSLRLSNAALS*STTTGTQPVS*.N YBR276C 1 1 3.8% 807 91685 7.1 U PPS1 SGDID:S000000480, Chr II from 760039-757616, reverse complement, Verified ORF, "Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle" * 22_g_01_itms.31440.31440.3 3.4642 0.2004 98.7 3634.6743 3641.7688 9614.5 356 4.395 18.3 1 N.LCSSPSEIVCFNNVDKNMVLCEKLELNKLTS.A Reverse_YEL065W 1 1 3.8% 628 70562 8.1 U SIT1 SGDID:S000000791, Chr V from 27657-29543, Verified ORF, "Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p" * 22_g_01_itms.27918.27918.2 2.4124 0.24 98.4 2603.612 2602.4246 11511.7 234 4.907 26.1 1 T.FPSGYAQAALTPDSIRKSIEKPLI.N YIL099W 1 1 3.8% 549 61463 5.1 U SGA1 SGDID:S000001361, Chr IX from 178001-179650, Verified ORF, "Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation" * 22_g_01_itms.22259.22259.2 2.4816 0.1292 91.8 2280.5522 2281.2056 9489.8 36 4.349 32.5 1 Y.NKLLGMLSVGFGFAWALENIT.I YBR078W 1 1 3.8% 468 48237 5.1 U ECM33 SGDID:S000000282, Chr II from 393118-393175,393506-394854, Verified ORF, "GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; similar to Sps2p and Pst1p" * 22_g_01_itms.02655.02655.3 2.8483 0.2742 98.8 1948.1344 1947.0472 7948.2 173 4.519 32.4 1 K.KITGDLNMQELIILTSAS.F YJL200C 1 1 3.7% 789 86583 7.0 U YJL200C SGDID:S000003736, Chr X from 58813-56444, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.26846.26846.3 3.5513 0.2359 99.4 3026.2444 3028.305 8506.3 234 4.784 22.3 1 V.EYFGEGVSTLSCTGMATICNMGAEIGATT.S YFR053C 1 2 3.7% 485 53738 5.5 U HXK1 SGDID:S000001949, Chr VI from 255036-253579, reverse complement, Verified ORF, "Hexokinase isoenzyme 1, a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves the hexokinase Hxk2p" * 22_g_01_itms.12510.12510.2 3.514 0.3758 100.0 2183.3523 2182.9668 8930.6 1 6.614 52.9 2 R.IEDDPFENLEDTDDIFQK.D Reverse_YKR034W 1 1 3.7% 269 30166 8.6 U DAL80 SGDID:S000001742, Chr XI from 506540-507349, Verified ORF, "Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Deh1p" * 22_g_01_itms.32346.32346.2 1.0629 0.0651 90.9 1063.7122 1064.4868 1957.8 59 4.323 50.0 1 F.LGCANCLVTG.H Reverse_YML109W 1 1 3.6% 942 105496 5.4 U ZDS2 SGDID:S000004577, Chr XIII from 51640-54468, Verified ORF, "Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; paralog of Zds1p" * 22_g_01_itms.21860.21860.3 3.8405 0.253 96.6 3870.6243 3866.6282 9194.9 83 4.919 18.9 1 A.TPSTNDEREAGIDEELGATTDRS*ISSHVEVDY*NV.D YNL287W 1 1 3.6% 935 104831 5.1 U SEC21 SGDID:S000005231, Chr XIV from 91994-94801, Verified ORF, "Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo" * 22_g_01_itms.16435.16435.3 4.511 0.2866 100.0 3902.5745 3895.6987 9510.1 1 5.771 26.5 1 K.EINPDTNEPFDGDEGFQDEYEIDSIFLNAGDYVK.S YLR451W 1 1 3.6% 886 100153 6.1 U LEU3 SGDID:S000004443, Chr XII from 1036089-1038749, Verified ORF, "Zinc-finger transcription factor that regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; positively regulated by alpha-isopropylmalate, an intermediate in leucine biosynthesis" * 22_g_01_itms.14160.14160.3 4.1841 0.1398 93.9 3814.8542 3813.9988 8819.9 2 4.031 21.8 1 S.QEEKQPLLHVLNQQLSQLEISLEENNLDDIRK.F Reverse_YPL032C 1 1 3.6% 825 92144 8.7 U SVL3 SGDID:S000005953, Chr XVI from 491362-488885, reverse complement, Verified ORF, "Protein of unknown function, mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck" * 22_g_01_itms.38940.38940.3 3.0003 0.145 93.8 3032.5745 3031.2913 7846.6 1 4.823 26.7 1 G.NDTSPSAMNAMQSSS*SLTPTSPASVSTTPP.P YMR297W 1 1 3.6% 532 59802 4.7 U PRC1 SGDID:S000004912, Chr XIII from 861921-863519, Verified ORF, "Vacuolar carboxypeptidase Y (proteinase C), involved in protein degradation in the vacuole and required for full protein degradation during sporulation" * 22_g_01_itms.21848.21848.2 3.7959 0.3657 100.0 2428.5122 2427.1548 10809.7 1 6.715 50.0 1 K.DVYNFLELFFDQFPEYVNK.G YER060W 1 1 3.6% 528 58050 5.5 U FCY21 SGDID:S000000862, Chr V from 274565-276151, Verified ORF, "Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function" * 22_g_01_itms.12522.12522.2 1.8554 0.1923 95.6 2048.7922 2047.9078 8626.8 2 4.535 38.9 1 S.FGSTVYGFAAGWTTYAADY.T YOL059W 1 2 3.6% 440 49422 7.1 U GPD2 SGDID:S000005420, Chr XV from 217125-218447, Verified ORF, "NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria" * 22_g_01_itms.15722.15722.2 4.5234 0.3477 100.0 1937.1921 1935.8091 7962.4 1 6.905 70.0 2 R.MEDLPEMIEELDIDDE.- YCL044C 1 1 3.6% 417 47156 9.3 U MGR1 SGDID:S000000549, Chr III from 48364-47111, reverse complement, Verified ORF, "Subunit, with Yme1p, of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; required for growth of cells lacking the mitochondrial genome" * 22_g_01_itms.31789.31789.3 2.229 0.1564 93.4 1526.1843 1524.6641 7226.8 268 3.968 37.5 1 -.MAVFTPPSGNSNSTD.H YER028C 1 1 3.6% 394 43119 9.9 U MIG3 SGDID:S000000830, Chr V from 211875-210691, reverse complement, Verified ORF, "Probable transcriptional repressor involved in response to toxic agents such as hydroxyurea that inhibit ribonucleotide reductase; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes" * 22_g_01_itms.10045.10045.3 2.7427 0.1545 91.9 1831.3143 1827.8383 7367.6 90 3.884 36.5 1 N.DQRPFRCEICSRGF.H YNL243W 1 1 3.5% 968 108996 5.5 U SLA2 SGDID:S000005187, Chr XIV from 188052-190958, Verified ORF, "Transmembrane actin-binding protein involved in membrane cytoskeleton assembly and cell polarization; adaptor protein that links actin to clathrin and endocytosis; present in the actin cortical patch of the emerging bud tip; dimer in vivo" * 22_g_01_itms.27439.27439.3 2.8859 0.0976 94.1 3839.5144 3836.0051 7699.1 287 4.056 15.9 1 S.MATLVTNSKAYAVTTLPQEQSDQILTLVKRCARE.A Reverse_YBR276C 1 1 3.5% 807 91685 7.1 U PPS1 SGDID:S000000480, Chr II from 760039-757616, reverse complement, Verified ORF, "Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle" * 22_g_01_itms.11455.11455.3 3.4759 0.2147 91.5 3309.6243 3309.4792 6179.3 104 4.753 22.2 1 I.ES*PSSCLNLPTNNKFFENDLLPTGILT*Q.P YBL099W 1 1 3.5% 545 58618 9.0 U ATP1 SGDID:S000000195, Chr II from 37050-38687, Verified ORF, "Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis" * 22_g_01_itms.45655.45655.3 3.1183 0.2078 92.4 2134.2544 2135.149 7520.7 218 4.769 31.9 1 A.PGILPRRSVHEPVQT*GLKA.V Reverse_YCR045C 1 1 3.5% 491 55068 5.4 U YCR045C SGDID:S000000641, Chr III from 209605-208130, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.12089.12089.2 2.1412 0.1728 93.5 1844.9722 1842.0383 6835.8 205 4.426 31.2 1 S.GLSLNAVCKKGQPRSVK.T YER090W 1 1 3.4% 507 56768 5.9 U TRP2 SGDID:S000000892, Chr V from 337945-339468, Verified ORF, "Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p" * 22_g_01_itms.11563.11563.2 4.7669 0.3146 100.0 1844.6322 1841.9384 8440.0 1 6.49 59.4 1 K.TGPTEGIETDPLEILEK.E YBR293W 1 1 3.4% 474 51677 9.7 U VBA2 SGDID:S000000497, Chr II from 787001-788425, Verified ORF, "Permease of basic amino acids in the vacuolar membrane" * 22_g_01_itms.15546.15546.3 2.6634 0.0963 98.6 1902.0844 1905.0566 8001.9 5 4.507 40.0 1 S.YAYLFTLPLFFQIVLG.D YBR233W 1 1 3.4% 413 45782 7.7 U PBP2 SGDID:S000000437, Chr II from 683423-684664, Verified ORF, "RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length" * 22_g_01_itms.07725.07725.2 2.5204 0.1809 96.0 1493.2522 1490.8075 7144.2 2 4.642 57.7 1 F.MASQAAIMLISNKI.E YAL060W 1 1 3.4% 382 41538 6.7 U BDH1 SGDID:S000000056, Chr I from 35156-36304, Verified ORF, "NAD-dependent (2R,3R)-2,3-butanediol dehydrogenase, a zinc-containing medium-chain alcohol dehydrogenase, produces 2,3-butanediol from acetoin during fermentation and allows using 2,3-butanediol as a carbon source during aerobic growth" * 22_g_01_itms.29497.29497.2 1.7259 0.1976 95.4 1416.0122 1416.8214 3214.3 13 4.521 50.0 1 M.AKKLGVEVFNPSK.H YIR033W 1 1 3.3% 1113 127054 5.2 U MGA2 SGDID:S000001472, Chr IX from 416121-419462, Verified ORF, "ER membrane protein involved, with its homolog Spt23p, in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting" * 22_g_01_itms.24558.24558.3 3.1091 0.1556 95.1 4223.304 4218.923 11480.0 330 4.127 16.0 1 D.SVETDSNYSISRKYSQSSFNSSLLDNESLNENLFESQ.S YLR249W 2 25 3.3% 1044 115945 6.0 U YEF3 SGDID:S000004239, Chr XII from 636782-639916, Verified ORF, "Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP" * 22_g_01_itms.18803.18803.3 4.7987 0.3144 100.0 3847.1643 3845.6548 7076.9 6 6.224 22.7 22 K.RAVDNIPVGPNFDDEEDEGEDLCNCEFSLAYGAK.I * 22_g_01_itms.11849.11849.3 5.4703 0.412 100.0 3691.2544 3689.5535 4059.7 1 7.367 30.5 3 R.AVDNIPVGPNFDDEEDEGEDLCNCEFSLAYGAK.I YAL035W 1 3 3.3% 1002 112268 5.8 U FUN12 SGDID:S000000033, Chr I from 76428-79436, Verified ORF, "GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2" * 22_g_01_itms.23681.23681.3 4.8992 0.3808 100.0 3570.3542 3564.6348 11727.4 1 6.468 25.0 3 R.LLVVGPEDDEDELMDDVMDDLTGLLDSVDTTGK.G YKL064W 1 1 3.3% 969 109736 7.5 U MNR2 SGDID:S000001547, Chr XI from 317408-320317, Verified ORF, "Putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations" * 22_g_01_itms.26929.26929.3 3.4347 0.2198 91.7 3542.2744 3535.7603 10185.4 132 3.861 19.4 1 S.NDIIKSEDPSLKGAFIDHRPSMSQPREGPQSV.S YOR329C 1 1 3.3% 872 97305 7.8 U SCD5 SGDID:S000005856, Chr XV from 939344-936726, reverse complement, Verified ORF, "Protein required for normal cortical actin organization and endocytosis; multicopy suppressor of clathrin deficiency; acts as a targeting subunit for protein phosphatase type 1" * 22_g_01_itms.27836.27836.3 3.1324 0.1274 93.5 3131.6343 3129.622 9357.4 197 4.018 21.4 1 L.SHFKQIQTVDSASLPISSVFLQNGNTLPT.S Reverse_YOR090C 1 1 3.3% 572 63669 8.6 U PTC5 SGDID:S000005616, Chr XV from 492842-491124, reverse complement, Verified ORF, "Mitochondrially localized type 2C protein phosphatase; contains Mg2+/Mn2+-dependent casein phosphatase activity in vitro but in vivo substrates are unknown" * 22_g_01_itms.39736.39736.3 2.5258 0.2285 97.2 1998.8043 2000.1544 5676.7 223 4.23 30.6 1 S.GSLLAINLAKSHSSIFLKT.R YOR310C 1 3 3.3% 511 56956 8.9 U NOP58 SGDID:S000005837, Chr XV from 898355-896820, reverse complement, Verified ORF, "Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA" * 22_g_01_itms.10593.10593.2 4.5157 0.3286 100.0 1960.4922 1959.9398 5100.0 1 7.629 75.0 3 K.ASETDLSEILPEEIEER.V YPL231W 3 32 3.2% 1887 206945 5.4 U FAS2 SGDID:S000006152, Chr XVI from 108652-114315, Verified ORF, "Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains beta-ketoacyl reductase and beta-ketoacyl synthase activities" * 22_g_01_itms.25396.25396.3 6.0336 0.4019 100.0 3400.4343 3398.6167 7706.2 1 6.726 27.5 21 K.EFGTTPEKPEETPLEELAETFQDTFSGALGK.Q * 22_g_01_itms.21000.21000.3 5.1588 0.2612 100.0 3487.5544 3487.6758 7322.2 1 5.122 32.1 9 K.DWVENELEALKLEAEEIPSEDQNEFLLER.T * 22_g_01_itms.09243.09243.2 5.1946 0.4111 100.0 2162.2322 2161.03 3232.0 1 7.585 70.6 2 K.LEAEEIPSEDQNEFLLER.T YPL174C 1 1 3.2% 868 100290 5.8 U NIP100 SGDID:S000006095, Chr XVI from 222772-220166, reverse complement, Verified ORF, "Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)" * 22_g_01_itms.35499.35499.3 2.8875 0.1998 99.0 3288.9844 3292.718 11709.8 8 4.644 22.2 1 F.LYPSCDITLSSILTILFSDALFLRQDYK.R Reverse_YGR014W 1 1 3.1% 1306 133114 4.2 U MSB2 SGDID:S000003246, Chr VII from 516947-520867, Verified ORF, "Mucin family member at the head of the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate" * 22_g_01_itms.22483.22483.3 3.2827 0.2052 96.6 3608.4243 3604.4263 10420.9 85 4.217 17.3 1 E.LTGADQYSNGSSSSVGSNSNSGSGSSGSNSNSSSSGDSSG.G Reverse_YIL002C 1 1 3.1% 946 108430 6.7 U INP51 SGDID:S000001264, Chr IX from 353428-350588, reverse complement, Verified ORF, "Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth" * 22_g_01_itms.31819.31819.3 3.164 0.1825 98.1 3038.5144 3040.4426 8477.3 311 4.313 21.4 1 R.KPEPIPTPSHTPGAISDSGEFSEIVFADD.S Reverse_YNL325C 1 1 3.1% 879 101746 5.7 U FIG4 SGDID:S000005269, Chr XIV from 31377-28738, reverse complement, Verified ORF, "Protein required for efficient mating, member of a family of eukaryotic proteins that contain a domain homologous to Sac1p" * 22_g_01_itms.26690.26690.3 2.6897 0.0157 92.3 3089.1543 3083.51 8576.9 315 3.916 22.1 1 Q.LAITDGLDHFLETLINVIDSDYELYSN.D Reverse_YJL090C 1 1 3.1% 764 87241 9.0 U DPB11 SGDID:S000003626, Chr X from 264967-262673, reverse complement, Verified ORF, "Essential BRCT repeat protein, required on the prereplicative complex at replication origins for loading DNA polymerases to initiate DNA synthesis, also required for S/M checkpoint control" * 22_g_01_itms.46021.46021.3 1.8686 0.1488 97.9 2791.8245 2790.352 7027.2 33 4.297 19.6 1 D.NHSSTDKIVFNQYNYSSCGLKKIS.R YKL217W 1 1 3.1% 616 69376 5.9 U JEN1 SGDID:S000001700, Chr XI from 22234-24084, Verified ORF, "Lactate transporter, required for uptake of lactate and pyruvate; expression is derepressed by transcriptional activator Cat8p under nonfermentative growth conditions, and repressed in the presence of glucose, fructose, and mannose" * 22_g_01_itms.11233.11233.2 2.3113 0.1121 92.0 2096.372 2092.1958 5271.8 32 4.368 33.3 1 G.LVLFVRSAGAVIFGLWTDK.S Reverse_YDR323C 1 1 3.1% 515 59443 8.4 U PEP7 SGDID:S000002731, Chr IV from 1114022-1112475, reverse complement, Verified ORF, "Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance" * 22_g_01_itms.33464.33464.3 2.3714 0.144 94.8 1865.5443 1865.022 4879.6 146 4.086 33.3 1 I.GSLPMRVDKNFKRGIF.L Reverse_YDL238C 1 1 3.1% 489 55204 6.0 U GUD1 SGDID:S000002397, Chr IV from 30454-28985, reverse complement, Verified ORF, "Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures" * 22_g_01_itms.07611.07611.2 2.1708 0.1605 94.1 1686.6322 1687.923 6159.4 9 4.436 46.4 1 N.RTKDKGIIDVVTVDE.P Reverse_YOL126C 1 1 3.1% 423 46170 7.3 U MDH2 SGDID:S000005486, Chr XV from 83057-81786, reverse complement, Verified ORF, "Cytoplasmic malate dehydrogenase, one of the three isozymes that catalyze interconversion of malate and oxaloacetate; involved in gluconeogenesis during growth on ethanol or acetate as carbon source; interacts with Pck1p and Fbp1p" * 22_g_01_itms.06770.06770.3 2.3895 0.1362 93.4 1312.5844 1314.5748 4183.7 24 4.017 33.3 1 L.CNEIGGAPSHSSV.S YJR132W 1 1 3.0% 1048 119983 4.7 U NMD5 SGDID:S000003893, Chr X from 669437-672583, Verified ORF, "Karyopherin, a carrier protein involved in nuclear import of proteins; importin beta homolog" * 22_g_01_itms.31315.31315.3 3.1837 0.166 93.4 3659.5144 3657.0508 10017.0 86 3.976 20.8 1 E.QCVVQKTTWKLVGPHYNVILQHVIFPLLKPT.A YEL032W 1 2 3.0% 971 107517 5.5 U MCM3 SGDID:S000000758, Chr V from 86937-89852, Verified ORF, "Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex" * 22_g_01_itms.08996.08996.3 4.6646 0.3229 100.0 3292.7944 3291.3872 10971.9 1 5.828 25.9 2 R.FQDDEQNAGEDDNDIMSPLPADEEAELQR.R Reverse_YNR047W 1 1 3.0% 893 100546 8.0 U YNR047W SGDID:S000005330, Chr XIV from 708525-711206, Uncharacterized ORF, "Putative protein kinase that, when overexpressed, interferes with pheromone-induced growth arrest; localizes to the cytoplasm; potential Cdc28p substrate" * 22_g_01_itms.18379.18379.3 3.5884 0.1337 91.1 3112.9143 3106.7014 10574.5 355 3.844 21.2 1 D.AAGMKCGLRKSENKTLLKKILDKCTRS.I YJL047C 1 1 3.0% 842 99326 7.6 U RTT101 SGDID:S000003583, Chr X from 352024-349496, reverse complement, Verified ORF, "Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; deletion phenotype suggests a role in anaphase progression; interacts with Mms22p and implicated in Mms22-dependent DNA repair; modified by the ubiquitin-like protein, Rub1p" * 22_g_01_itms.30315.30315.3 3.6267 0.252 98.6 3302.7244 3303.5242 8040.7 12 5.04 25.0 1 H.VLS*FLTRCDYSYEIIPKNWT*SFYKL.F YOL152W 1 2 3.0% 629 71997 9.1 U FRE7 SGDID:S000005512, Chr XV from 40747-42636, Verified ORF, "Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels" * 22_g_01_itms.22205.22205.2 2.337 0.1735 91.9 1985.1322 1983.0876 7039.4 67 4.342 33.3 2 Y.LGIPTSVGTFLVVMATTLY.T YMR279C 1 1 3.0% 540 59562 8.2 U YMR279C SGDID:S000004892, Chr XIII from 826350-824728, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.38101.38101.2 1.9804 0.1485 93.3 1928.0922 1927.882 8116.3 176 5.112 36.7 1 F.SIFKKKTSVQGT*DS*EI.D YKL221W 1 1 3.0% 473 52342 8.8 U MCH2 SGDID:S000001704, Chr XI from 6108-7529, Verified ORF, "Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport" * 22_g_01_itms.06757.06757.2 2.2213 0.1033 95.8 1146.5122 1147.596 5223.0 137 4.578 50.0 1 R.SLASGIGTAGSGLG.G YLR383W 1 1 2.9% 1114 128008 7.5 U SMC6 SGDID:S000004375, Chr XII from 885288-888632, Verified ORF, "Protein involved in structural maintenance of chromosomes; required for interchromosomal and sister chromatid recombination; homologous to S. pombe rad18" * 22_g_01_itms.22679.22679.3 3.7776 0.188 96.3 3786.8943 3782.9712 9210.1 260 4.181 19.4 1 Q.MRQELEQLEKANEKLREVNNSLVVSLQDVKNE.E YER110C 1 1 2.9% 1113 122601 4.6 U KAP123 SGDID:S000000912, Chr V from 382099-378758, reverse complement, Verified ORF, "Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1" * 22_g_01_itms.07009.07009.3 5.3312 0.3531 100.0 3582.8342 3580.4993 7559.1 1 6.351 29.8 1 K.VACEEIDVDDELNNEDETGENEENTPSSSAIR.L YNL233W 1 1 2.9% 892 100590 5.1 U BNI4 SGDID:S000005177, Chr XIV from 211923-214601, Verified ORF, "Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p" * 22_g_01_itms.23862.23862.3 2.4119 0.1558 91.9 2955.7744 2950.458 7846.9 6 3.887 24.0 1 N.MDLILGGDKQINTPLQEHVREDDDAK.N Reverse_YPR181C 1 1 2.9% 768 85385 5.7 U SEC23 SGDID:S000006385, Chr XVI from 899663-897357, reverse complement, Verified ORF, "GTPase-activating protein; component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle coat, involved in ER to Golgi transport and autophagy; stimulates the GDP-bound form of Sar1p" * 22_g_01_itms.27243.27243.2 2.4823 0.2257 97.2 2280.3123 2283.2283 8849.8 24 4.781 31.0 1 A.KHVAIRAMLVAAAEQDFSAAIA.P Reverse_YBR238C 1 1 2.9% 731 83761 7.5 U YBR238C SGDID:S000000442, Chr II from 697297-695102, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.13034.13034.2 3.0259 0.2981 97.8 2260.2522 2259.1199 3814.4 127 4.813 35.0 1 H.PVFHNAPSVQYTALAPNNFTA.P Reverse_YOR372C 1 1 2.9% 554 60523 9.5 U NDD1 SGDID:S000005899, Chr XV from 1036467-1034803, reverse complement, Verified ORF, "Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes" * 22_g_01_itms.38578.38578.2 1.9561 0.2357 95.7 1831.9922 1832.9507 6860.2 165 4.617 33.3 1 N.SKLLNNLDVNSFVNQQ.Q YKL211C 1 1 2.9% 484 53489 6.9 U TRP3 SGDID:S000001694, Chr XI from 38154-36700, reverse complement, Verified ORF, "Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p" * 22_g_01_itms.07833.07833.2 2.6836 0.2135 91.4 1561.4321 1560.7659 6481.1 8 4.331 53.8 1 R.NILNVSGGTWEENK.S Reverse_YNL287W 1 1 2.8% 935 104831 5.1 U SEC21 SGDID:S000005231, Chr XIV from 91994-94801, Verified ORF, "Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo" * 22_g_01_itms.10814.10814.3 3.1118 0.2759 98.2 3134.0942 3133.3584 2986.5 311 5.315 24.0 1 F.S*DTNSSIY*SSLKSELSPIDYFYKSEI.L Reverse_YPR029C 1 1 2.8% 832 93624 5.5 U APL4 SGDID:S000006233, Chr XVI from 626964-624466, reverse complement, Verified ORF, "Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport" * 22_g_01_itms.21615.21615.2 2.219 0.1997 92.1 2470.3323 2467.2986 10573.2 4 4.374 31.8 1 E.PSSLFGLSTLALSVAYKNPHHLD.N Reverse_YOR035C 1 1 2.8% 789 89553 5.6 U SHE4 SGDID:S000005561, Chr XV from 400103-397734, reverse complement, Verified ORF, "Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization" * 22_g_01_itms.16859.16859.2 2.6072 0.256 91.1 2556.5122 2560.3984 11137.0 11 5.06 33.3 1 F.LFPIANLAS*YKKFILGPNTFIL.M Reverse_YLR337C 1 1 2.8% 817 82594 9.9 U VRP1 SGDID:S000004329, Chr XII from 805106-802653, reverse complement, Verified ORF, "Proline-rich, actin-associated protein involved in cytoskeletal organization and cytokinesis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP)" * 22_g_01_itms.33258.33258.3 2.4101 0.1368 98.5 2089.4644 2090.165 6588.5 44 4.382 26.1 1 P.VAPAPSLPANPIPPAAVSPVAGP.I YGR178C 1 1 2.8% 722 78782 6.9 U PBP1 SGDID:S000003410, Chr VII from 853220-851052, reverse complement, Verified ORF, "Protein interacting with poly(A)-binding protein Pab1p; likely involved in control of the extent of mRNA polyadenylation; also interacts with Mkt1p to form a complex that may regulate translation of the HO mRNA" * 22_g_01_itms.10433.10433.2 3.9019 0.3964 100.0 2181.392 2181.02 7130.0 1 7.056 52.6 1 R.GIIIDDSGLDEEDLYSGVDR.R Reverse_YGL150C 1 1 2.7% 1489 171455 5.7 U INO80 SGDID:S000003118, Chr VII from 225578-221109, reverse complement, Verified ORF, "ATPase that forms a large complex, containing actin and several actin-related proteins, that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; shows similarity to the Snf2p family of ATPases" * 22_g_01_itms.33482.33482.3 2.3466 0.2065 94.3 4315.1045 4311.9883 12236.3 14 4.895 17.9 1 N.NGDENEDGNVTVVTDAHT*QDDKIAKGIAAAAAADKKRS*KQ.I YDL073W 1 1 2.7% 984 113891 7.9 U YDL073W SGDID:S000002231, Chr IV from 326613-329567, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.37026.37026.2 1.5721 0.3407 100.0 3255.112 3255.5784 4725.9 1 5.918 19.2 1 D.FHFDT*ILLLT*VSPQISSSIQTSLFSWK.K YCR026C 1 1 2.7% 742 84734 4.6 U NPP1 SGDID:S000000621, Chr III from 166334-164106, reverse complement, Verified ORF, "Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation" * 22_g_01_itms.09157.09157.2 2.2655 0.1846 90.4 2268.672 2268.1045 9189.7 38 4.282 31.6 1 R.KDYVSHAYLEGPMMAISLKD.S Reverse_YNL218W 1 1 2.7% 587 66544 8.4 U MGS1 SGDID:S000005162, Chr XIV from 238239-240002, Verified ORF, "Protein with DNA-dependent ATPase and ssDNA annealing activities involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP)" * 22_g_01_itms.15839.15839.2 2.2486 0.101 92.6 1891.2922 1887.9315 10228.9 135 4.379 33.3 1 A.VYLPDEGGQLMRALYY.L YIR019C 1 2 2.6% 1367 136110 4.3 U MUC1 SGDID:S000001458, Chr IX from 393672-389569, reverse complement, Verified ORF, "GPI-anchored cell surface glycoprotein required for diploid pseudohyphal formation and haploid invasive growth, transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p)" 22_g_01_itms.09542.09542.3 3.3936 0.1458 92.3 3309.2944 3313.4568 6792.0 56 3.913 19.1 2 T.TESSSAPVPTPSSSTTESSSAPAPTPSSSTTESSS.A YDR128W 1 1 2.6% 1148 130946 5.9 U YDR128W SGDID:S000002535, Chr IV from 709544-712990, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.29719.29719.3 3.4365 0.1658 98.6 3343.3743 3348.701 9496.3 353 4.49 20.7 1 K.QGSRAISSSPFHSRMPDIKVELLHDDIIEA.Y YCR067C 1 1 2.6% 1065 114079 4.7 U SED4 SGDID:S000000663, Chr III from 236317-233120, reverse complement, Verified ORF, "Integral endoplasmic reticulum membrane protein, functions as a positive regulator of Sar1p probably through inhibition of GTPase activation by Sec23p; binds Sec16p, participates in vesicle formation, similar to Sec12p" * 22_g_01_itms.09566.09566.3 3.9775 0.2174 90.0 3090.5044 3091.355 6946.7 135 4.74 21.3 1 S.VASNLIPNEEILS*TSAS*QDSISSHPSTF.S YHR027C 1 1 2.6% 993 109492 4.6 U RPN1 SGDID:S000001069, Chr VIII from 164704-161723, reverse complement, Verified ORF, "Non-ATPase base subunit of the 19S regulatory particle of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein?protein interactions" * 22_g_01_itms.30176.30176.2 1.9095 0.2047 96.5 2587.652 2583.2942 8390.2 245 4.669 24.0 1 L.YVDEPEVKAGALLGIGISASGVHDGE.V YMR031C 1 1 2.6% 843 93345 6.3 U YMR031C SGDID:S000004633, Chr XIII from 334742-332211, reverse complement, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * 22_g_01_itms.30946.30946.2 2.0781 0.1592 90.5 2373.0322 2370.0374 9885.7 1 4.31 35.7 1 D.SLEHTFSGFSQGSSIEDDQDAI.S YFL009W 1 1 2.6% 779 86090 7.1 U CDC4 SGDID:S000001885, Chr VI from 116139-118478, Verified ORF, "F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p" * 22_g_01_itms.10197.10197.2 2.0438 0.1125 90.0 2002.3522 1998.9005 7809.8 137 4.264 31.6 1 T.GAASVDNDGLHNLTDISNDA.E YDR172W 2 7 2.6% 685 76551 7.0 U SUP35 SGDID:S000002579, Chr IV from 808319-810376, Verified ORF, "Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype" * 22_g_01_itms.10495.10495.2 5.1549 0.5166 100.0 2068.912 2068.8657 8684.2 1 9.807 67.6 5 K.EQEEEVDDEVVNDMFGGK.D * 22_g_01_itms.10515.10515.3 3.7712 0.1525 99.0 2071.1042 2068.8657 8496.4 1 4.607 38.2 2 K.EQEEEVDDEVVNDMFGGK.D Reverse_YIR006C 1 1 2.5% 1480 160266 5.3 U PAN1 SGDID:S000001445, Chr IX from 369905-365463, reverse complement, Verified ORF, "Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; previously thought to be a subunit of poly(A) ribonuclease" * 22_g_01_itms.20176.20176.3 3.7525 0.2076 98.9 3762.2344 3761.7612 7767.9 36 4.674 19.4 1 M.VSGYGGTYQSQLAGGTLQPGLNVGFSTQPMMGTTQPM.M YDR069C 1 1 2.5% 926 105192 8.2 U DOA4 SGDID:S000002476, Chr IV from 587716-584936, reverse complement, Verified ORF, "Ubiquitin hydrolase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole" * 22_g_01_itms.30620.30620.2 2.2503 0.2331 95.5 2555.3323 2556.226 7744.1 21 4.495 27.3 1 M.SPSIRHSMDDSMKEMLVAPTPLN.H Reverse_YOL006C 1 1 2.5% 769 89995 8.7 U TOP1 SGDID:S000005366, Chr XV from 315387-313078, reverse complement, Verified ORF, "Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination" * 22_g_01_itms.07261.07261.3 2.9718 0.1915 95.3 2094.3245 2092.1038 6173.3 5 4.136 34.7 1 V.SLRPDIYNIKSTGLSVQSN.E YLL018C 1 2 2.5% 557 63516 6.6 U DPS1 SGDID:S000003941, Chr XII from 111574-109901, reverse complement, Verified ORF, "Cytoplasmic aspartyl-tRNA synthetase, homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation" * 22_g_01_itms.06708.06708.2 2.452 0.1847 96.4 1626.0922 1625.7183 8207.3 25 4.673 50.0 2 K.EIGDFEDLSTENEK.F YOR124C 1 1 2.4% 1272 146354 5.2 U UBP2 SGDID:S000005650, Chr XV from 558642-554824, reverse complement, Verified ORF, "Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins, cleaves at the C terminus of ubiquitin fusions; capable of cleaving polyubiquitin and possesses isopeptidase activity" * 22_g_01_itms.35758.35758.3 3.1331 0.0429 91.7 3564.7444 3557.7434 6042.2 67 3.868 21.7 1 E.CNISELNEATLLSIYKYETSNKSQVTSNHLT.N YOR330C 1 1 2.4% 1254 143502 8.8 U MIP1 SGDID:S000005857, Chr XV from 943380-939616, reverse complement, Verified ORF, "Catalytic subunit of the mitochondrial DNA polymerase" * 22_g_01_itms.13729.13729.3 3.0301 0.0316 94.3 3677.3643 3672.0205 11284.8 7 4.058 22.4 1 I.KDIVLLKDKPDFYLKDPWLSQLDWTTKPLR.L Reverse_YDR507C 1 1 2.4% 1142 129858 9.2 U GIN4 SGDID:S000002915, Chr IV from 1465776-1462348, reverse complement, Verified ORF, "Protein kinase involved in bud growth and assembly of the septin ring, proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Kcc4p and Hsl1p" * 22_g_01_itms.22047.22047.2 2.1592 0.134 95.9 3241.2722 3239.6582 7350.4 3 4.646 30.8 1 R.IKQVSKNLPPEMDDVVKERKEQDEEQK.H YGR162W 1 2 2.4% 952 107101 6.0 U TIF4631 SGDID:S000003394, Chr VII from 824064-826922, Verified ORF, "Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4632p" * 22_g_01_itms.14484.14484.2 3.0995 0.2659 99.3 2554.3323 2553.2036 11340.8 2 4.979 31.8 2 K.DATPIEDVFSFNYPEGIEGPDIK.Y YJL094C 1 1 2.4% 873 97111 6.1 U KHA1 SGDID:S000003630, Chr X from 254358-251737, reverse complement, Verified ORF, "Putative K+/H+ antiporter" * 22_g_01_itms.35488.35488.2 2.1019 0.2419 95.2 2331.8523 2328.2917 11727.4 289 5.187 27.5 1 E.IVVLT*VGLNAGIISRKIFGMF.V YLR234W 1 1 2.4% 656 74371 8.5 U TOP3 SGDID:S000004224, Chr XII from 609785-611755, Verified ORF, "DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination" * 22_g_01_itms.02102.02102.3 2.3511 0.1234 90.7 1855.0443 1857.8342 7418.2 170 3.82 30.0 1 G.IDFSHDSHGWGKCAIQ.E Reverse_YNL088W 1 1 2.3% 1428 164214 7.0 U TOP2 SGDID:S000005032, Chr XIV from 457706-461992, Verified ORF, "Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis" * 22_g_01_itms.24744.24744.3 3.6436 0.146 92.6 3654.0842 3658.564 10944.0 151 3.927 19.5 1 Y.LEEPGNIVNETDEHSSEEDEEDSTAGKVDNIQE.A Reverse_YBL085W 1 1 2.3% 980 109295 9.1 U BOI1 SGDID:S000000181, Chr II from 63873-66815, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" * 22_g_01_itms.17576.17576.2 2.6863 0.1818 97.5 2680.2322 2678.4492 11190.8 46 4.782 27.3 1 V.RPETFTLGKKSGPQPPVLKFCYK.G YOR038C 1 1 2.3% 875 98445 8.3 U HIR2 SGDID:S000005564, Chr XV from 405387-402760, reverse complement, Verified ORF, "Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3, and H4 genes; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p" * 22_g_01_itms.29269.29269.3 2.3759 0.0141 91.9 1952.0343 1950.0773 7952.0 178 3.878 31.6 1 S.ASAAPIPNDIGRSAVGKKPT.K Reverse_YGR196C 1 1 2.3% 817 90798 4.6 U FYV8 SGDID:S000003428, Chr VII from 892191-889738, reverse complement, Verified ORF, "Protein of unknown function, required for survival upon exposure to K1 killer toxin" * 22_g_01_itms.36612.36612.3 2.1843 0.1536 93.5 1943.3043 1944.0038 3391.5 25 4.021 34.7 1 K.TENAVPKPKAVKDQGSSTS.N YBR142W 1 2 2.3% 773 87048 8.4 U MAK5 SGDID:S000000346, Chr II from 528311-530632, Verified ORF, "Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits" * 22_g_01_itms.07471.07471.2 4.04 0.2942 100.0 1989.7522 1986.8069 6701.1 1 6.691 58.8 2 K.AADELGIDVDS*DEDDISK.S YOL057W 1 1 2.3% 711 80510 6.3 U YOL057W SGDID:S000005418, Chr XV from 220765-222900, Uncharacterized ORF, "Putative metalloprotease" * 22_g_01_itms.37770.37770.3 2.2741 0.0705 91.9 1563.1144 1564.8447 6313.0 1 3.886 43.3 1 L.GNILSAAAKSSSKHPP.S YOL141W 1 1 2.3% 695 78975 7.0 U PPM2 SGDID:S000005501, Chr XV from 56450-58537, Verified ORF, "Putative carboxyl methyl transferase, has similarity to Ppm1p but biochemical activity not yet demonstrated" * 22_g_01_itms.08841.08841.3 2.3543 0.081 92.2 1843.4343 1843.037 6156.2 9 3.909 40.0 1 I.ILEQLIPKGPFEPFSK.Q YDL063C 1 1 2.3% 620 70164 5.2 U YDL063C SGDID:S000002221, Chr IV from 340134-338272, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 22_g_01_itms.30047.30047.3 1.2526 0.1434 92.2 1595.2444 1593.7073 1854.8 220 3.907 36.5 1 M.TNSLFQIYGDASYD.Y Reverse_YPL194W 1 1 2.3% 612 69694 6.6 U DDC1 SGDID:S000006115, Chr XVI from 179276-181114, Verified ORF, "DNA damage checkpoint protein, part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate" * 22_g_01_itms.22793.22793.2 2.3348 0.2001 92.8 1543.0122 1545.7471 8113.3 11 4.395 46.2 1 R.PKEVQTLGLGDEME.K YNL275W 1 1 2.3% 576 65028 7.8 U YNL275W SGDID:S000005219, Chr XIV from 119268-120998, Verified ORF, "Plasma membrane protein that binds HCO3-, I-, Br-, NO3- and Cl- ; putative boron efflux transporter with similarity to A. thaliana BOR1 which is a known boron efflux transporter" * 22_g_01_itms.38338.38338.3 1.8963 0.1605 92.3 1342.4644 1342.7041 4359.8 259 3.906 29.2 1 L.VCLGEIPQAVLSG.L YLR259C 1 2 2.3% 572 60752 5.3 U HSP60 SGDID:S000004249, Chr XII from 665004-663286, reverse complement, Verified ORF, "Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; similarity to groEL" * 22_g_01_itms.08732.08732.2 3.9917 0.3256 100.0 1522.1522 1521.7107 9592.7 1 6.936 62.5 2 R.TLEDELEVTEGMR.F Reverse_YGL197W 2 2 2.2% 1487 167074 7.7 U MDS3 SGDID:S000003165, Chr VII from 124703-129166, Verified ORF, "Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions" * 22_g_01_itms.32540.32540.2 1.6366 0.2548 99.6 1092.0322 1092.565 2153.6 64 5.214 50.0 1 F.PKGSASSTRTT.S * 22_g_01_itms.35388.35388.2 2.5326 0.2235 96.9 2453.4722 2452.432 9928.0 1 4.764 32.5 1 L.SLSEEKKALISYLVELILKYL.L Reverse_YBL007C 1 1 2.2% 1244 135848 5.9 U SLA1 SGDID:S000000103, Chr II from 216369-212635, reverse complement, Verified ORF, "Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; contains 3 SH3 domains; interacts with proteins regulating actin dynamics and with proteins required for endocytosis" * 22_g_01_itms.11240.11240.2 2.6212 0.1696 90.5 2491.8323 2488.3298 9051.4 12 4.318 28.8 1 D.LPAPASSVPASSVPAAPVTTGGTKVPE.L Reverse_YOR109W 1 1 2.2% 1107 124577 7.2 U INP53 SGDID:S000005635, Chr XV from 525278-528601, Verified ORF, "Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in a TGN (trans Golgi network)-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches" * 22_g_01_itms.26558.26558.2 2.0082 0.1732 90.2 2439.4321 2443.302 10607.2 186 4.281 26.1 1 V.TEGPCPQAVKSKGTIAGVFLLGNV.E Reverse_YBL052C 1 1 2.2% 831 97582 6.5 U SAS3 SGDID:S000000148, Chr II from 121877-119382, reverse complement, Verified ORF, "Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; sas3 gcn5 double mutation confers lethality" * 22_g_01_itms.14191.14191.2 2.3165 0.1807 95.3 2246.3523 2249.9258 6459.4 24 4.477 35.3 1 N.KYDCMYETLSSVTDEFFD.T YBR202W 1 1 2.2% 845 94875 5.3 U CDC47 SGDID:S000000406, Chr II from 625767-628304, Verified ORF, "Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase" * 22_g_01_itms.38677.38677.3 2.3032 0.3188 97.6 2279.6643 2283.1274 3847.1 160 4.982 30.6 1 K.VANRELNS*VIIDLDDILQY.Q Reverse_YGR261C 1 1 2.2% 809 91607 5.8 U APL6 SGDID:S000003493, Chr VII from 1016756-1014327, reverse complement, Verified ORF, "Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools" * 22_g_01_itms.35359.35359.3 1.9655 0.1316 94.0 1973.5443 1972.0616 4284.7 36 4.045 30.9 1 E.LRASVIFDDSRATPKPAQ.L Reverse_YJL168C 1 1 2.2% 733 84540 8.5 U SET2 SGDID:S000003704, Chr X from 104421-102220, reverse complement, Verified ORF, "Histone methyltransferase with a role in transcriptional elongation, methylates a lysine residue of histone H3; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p" * 22_g_01_itms.17431.17431.3 2.8249 0.0724 96.3 1983.4744 1985.1198 6943.2 67 4.182 36.7 1 G.NEHIIEWGPPLRVRRL.D Reverse_YKR098C 1 1 2.2% 717 82703 8.4 U UBP11 SGDID:S000001806, Chr XI from 634817-632664, reverse complement, Verified ORF, "Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins" * 22_g_01_itms.33919.33919.3 2.6145 0.196 98.6 1700.1244 1701.9064 7929.0 2 4.508 40.0 1 E.IVPLNYAKPASSEIAE.I YJR016C 1 1 2.2% 585 62861 7.8 U ILV3 SGDID:S000003777, Chr X from 466122-464365, reverse complement, Verified ORF, "Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids" * 22_g_01_itms.05081.05081.2 4.3911 0.2596 100.0 1433.1322 1431.6604 9162.2 3 5.278 70.8 1 R.DGDEIIIDADNNK.I YHR019C 1 1 2.2% 554 62207 5.8 U DED81 SGDID:S000001061, Chr VIII from 143551-141887, reverse complement, Verified ORF, "Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA" * 22_g_01_itms.11501.11501.2 2.334 0.1744 99.3 1386.5521 1384.6129 7091.8 1 5.173 63.6 1 R.IDDMDELMAGFK.R YGL133W 1 1 2.1% 1264 145642 5.7 U ITC1 SGDID:S000003101, Chr VII from 257712-261506, Verified ORF, "Component of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1" * 22_g_01_itms.16196.16196.3 3.1 0.1655 90.4 3111.6543 3110.449 9263.5 348 3.805 21.0 1 L.FINEEGDWSCIVVENWIIDDKGVLME.R YBL051C 1 1 2.1% 668 73776 6.2 U PIN4 SGDID:S000000147, Chr II from 124762-122756, reverse complement, Verified ORF, "Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage" * 22_g_01_itms.10188.10188.2 1.9973 0.2023 95.7 1378.7922 1377.661 7068.0 56 4.604 46.2 1 N.NNSASSTPKISSQG.Q YKL182W 2 4 2.0% 2051 228689 5.9 U FAS1 SGDID:S000001665, Chr XI from 100676-106831, Verified ORF, "Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities" * 22_g_01_itms.09567.09567.2 3.5516 0.427 100.0 2498.4521 2497.2349 5403.9 7 6.907 32.6 2 K.ILPEPTEGFAADDEPTTPAELVGK.F * 22_g_01_itms.11245.11245.2 4.3468 0.3928 100.0 1855.5521 1853.8921 3823.6 1 7.534 73.3 2 K.DIQIPVYDTFDGSDLR.V YNL091W 1 1 2.0% 1240 141514 5.4 U NST1 SGDID:S000005035, Chr XIV from 452410-456132, Verified ORF, "Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1" * 22_g_01_itms.23409.23409.3 3.4156 0.1213 91.3 3237.8643 3237.876 10048.0 34 3.846 25.0 1 N.NRLKLLQELEEEKRKKREKEEKKQK.K Reverse_YJL076W 1 1 2.0% 1189 128531 7.8 U NET1 SGDID:S000003612, Chr X from 295161-298730, Verified ORF, "Core subunit of the RENT complex, which is a complex involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure" * 22_g_01_itms.35298.35298.2 1.984 0.2088 92.4 2555.2522 2559.5322 10227.7 1 4.375 32.6 1 N.KKAGALLQAARKAASRPTRQPLIN.R Reverse_YML010W 1 1 2.0% 1063 115650 5.2 U SPT5 SGDID:S000004470, Chr XIII from 247677-250868, Verified ORF, "Protein that forms a complex with Spt4p and mediates both activation and inhibition of transcription elongation; Spt4p-Spt5p complex also plays a role in pre-mRNA processing" * 22_g_01_itms.08623.08623.2 2.1757 0.2047 96.7 2484.4722 2480.286 5526.1 40 4.71 30.0 1 E.DFKGYDLRPVIKLMVELNNES.I Reverse_YNL327W 1 1 2.0% 1041 108495 4.8 U EGT2 SGDID:S000005271, Chr XIV from 24047-27172, Verified ORF, "Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner" * 22_g_01_itms.35458.35458.3 2.4073 0.3268 99.7 2075.3643 2074.9417 4195.2 111 5.07 30.0 1 T.TFTSNSSSPASLSISSSSAQE.S YJL130C 3 6 1.9% 2214 245123 5.9 U URA2 SGDID:S000003666, Chr X from 172285-165641, reverse complement, Verified ORF, "Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP" * 22_g_01_itms.07037.07037.2 3.3343 0.4123 100.0 1587.8522 1586.6896 7936.9 1 7.408 57.7 2 K.ELTSMDEAESFAEK.V * 22_g_01_itms.13771.13771.2 3.3626 0.3253 100.0 2957.9321 2957.4268 4403.5 3 5.763 32.7 1 R.FSITEEAIADNLDAAEDAIPEQPLEQK.L * 22_g_01_itms.13865.13865.3 4.0022 0.1648 98.7 2958.2944 2957.4268 4233.2 68 4.388 25.0 3 R.FSITEEAIADNLDAAEDAIPEQPLEQK.L Reverse_YNL273W 1 1 1.9% 1238 141120 6.1 U TOF1 SGDID:S000005217, Chr XIV from 122883-126599, Verified ORF, "Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase" * 22_g_01_itms.07508.07508.3 2.9812 0.1336 90.1 2671.9744 2672.426 6226.7 13 3.797 28.3 1 K.NWKKSDDLKQLAIKEDVLAQSGSV.T Reverse_YDL215C 1 1 1.9% 1092 124332 5.7 U GDH2 SGDID:S000002374, Chr IV from 73919-70641, reverse complement, Verified ORF, "NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels" * 22_g_01_itms.25222.25222.2 2.1278 0.1686 94.3 2423.652 2426.373 8897.5 2 4.437 35.0 1 E.DKNEVSRTTKIVPGEREKVLK.L YGR100W 1 1 1.9% 950 109259 5.4 U MDR1 SGDID:S000003332, Chr VII from 690249-693101, Verified ORF, "Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function" * 22_g_01_itms.08858.08858.3 2.9828 0.2048 97.4 2190.3542 2186.2532 4917.3 404 4.235 29.4 1 P.MFRKLIRIGVPNRMRGEI.W YMR275C 1 1 1.9% 976 109174 8.7 U BUL1 SGDID:S000004888, Chr XIII from 818580-815650, reverse complement, Verified ORF, "Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex, functional homolog of Bul2p, disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases" * 22_g_01_itms.00240.00240.2 2.0824 0.1049 95.5 2103.4321 2107.1511 9438.3 6 4.484 36.1 1 L.SINYTIDARIVGKDQKASK.L YLR289W 1 1 1.9% 645 73188 9.2 U GUF1 SGDID:S000004280, Chr XII from 715091-717028, Verified ORF, "Mitochondrial GTPase of unknown function, similar to E. coli elongation factor-type GTP-binding protein LepA and to LK1236.1 from Caenorhabditis elegans" * 22_g_01_itms.35418.35418.2 1.847 0.1033 97.0 1268.0122 1266.6079 3552.7 3 4.734 54.5 1 V.AHVDHGKSTLSD.R Reverse_YPL231W 2 2 1.8% 1887 206945 5.4 U FAS2 SGDID:S000006152, Chr XVI from 108652-114315, Verified ORF, "Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains beta-ketoacyl reductase and beta-ketoacyl synthase activities" * 22_g_01_itms.37658.37658.2 1.8969 0.1081 91.8 1470.7522 1469.8328 7328.3 333 4.355 36.7 1 Q.LLGQLVEAGISGKGAG.T * 22_g_01_itms.13163.13163.2 2.4082 0.1861 93.3 1427.8722 1429.7803 6118.5 101 4.422 38.2 1 A.AAAPAAAAVPAPAAAAPA.P YOR116C 1 1 1.8% 1460 162301 8.2 U RPO31 SGDID:S000005642, Chr XV from 544145-539763, reverse complement, Verified ORF, "RNA polymerase III subunit C160, part of core enzyme; similar to bacterial beta-prime subunit" * 22_g_01_itms.08940.08940.3 2.8423 0.051 93.5 2902.5244 2900.582 8607.5 50 4.02 24.0 1 K.TFHFAGVASMNVTLGVPRIKEIINASK.V YBL076C 1 1 1.8% 1072 122983 6.1 U ILS1 SGDID:S000000172, Chr II from 84259-81041, reverse complement, Verified ORF, "Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A" * 22_g_01_itms.11579.11579.2 3.0927 0.1764 99.3 1986.5322 1983.9988 4488.9 1 5.026 58.3 1 K.NGVISEDSVLPNAIDDLGR.F Reverse_YBR038W 1 1 1.8% 963 109882 8.6 U CHS2 SGDID:S000000242, Chr II from 311897-314788, Verified ORF, "Chitin synthase II, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for the synthesis of chitin in the primary septum during cytokinesis" * 22_g_01_itms.32575.32575.2 2.3288 0.172 92.9 2181.8523 2181.8909 9203.6 55 5.184 37.5 1 A.Y*RNPAVPS*NHASDRYFQ.T YBR086C 1 1 1.8% 946 105854 9.0 U IST2 SGDID:S000000290, Chr II from 423035-420195, reverse complement, Verified ORF, "Plasma membrane protein that may be involved in osmotolerance, localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process" * 22_g_01_itms.34887.34887.3 2.4962 0.2619 92.1 1781.8744 1778.7655 2885.3 274 4.787 35.9 1 K.VPTVGS*YGVAGATLPET*.I Reverse_YKL171W 1 1 1.8% 928 103956 8.8 U YKL171W SGDID:S000001654, Chr XI from 127480-130266, Uncharacterized ORF, "Putative protein of unknown function; predicted protein kinase; implicated in proteasome function; epitope-tagged protein localizes to the cytoplasm" * 22_g_01_itms.07741.07741.3 2.8184 0.1534 95.1 2170.8542 2169.0916 7429.1 206 4.133 35.9 1 R.CREAFPIKSRKFEDWSI.V Reverse_YLR386W 1 1 1.8% 880 99772 5.5 U VAC14 SGDID:S000004378, Chr XII from 893628-896270, Verified ORF, "Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; activator of Fab1p" * 22_g_01_itms.06762.06762.3 2.4732 0.1163 91.4 1840.5243 1844.2029 5422.1 161 3.856 31.7 1 S.LLKLLVSLIKSLFPIF.V Reverse_YAL041W 1 1 1.8% 854 96940 8.5 U CDC24 SGDID:S000000039, Chr I from 62841-65405, Verified ORF, "Guanine nucleotide exchange factor (GEF or GDP-release factor) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing" * 22_g_01_itms.45170.45170.2 2.1248 0.1985 94.8 1531.2322 1533.9216 7369.3 87 4.465 35.7 1 S.ASISASTSSKKKLIL.S Reverse_YHR072W 1 1 1.8% 731 83461 6.5 U ERG7 SGDID:S000001114, Chr VIII from 239100-241295, Verified ORF, "Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis" * 22_g_01_itms.07407.07407.2 2.346 0.1788 90.7 1501.6921 1503.7517 6970.0 3 4.323 58.3 1 Q.CPFIGSDPEQLLK.F YHL024W 1 1 1.8% 713 80109 5.8 U RIM4 SGDID:S000001016, Chr VIII from 56647-58788, Verified ORF, "Putative RNA-binding protein required for the expression of early and middle sporulation genes" * 22_g_01_itms.38005.38005.2 1.7755 0.1444 96.5 1415.2722 1412.7035 6240.4 52 4.665 50.0 1 H.NRKFSGKNGGSNF.N Reverse_YNL008C 1 1 1.8% 669 76741 6.5 U ASI3 SGDID:S000004953, Chr XIV from 618221-616212, reverse complement, Verified ORF, "Putative integral membrane E3 ubiquitin ligase; genetic interactions suggest a role in negative regulation of amino acid uptake" * 22_g_01_itms.39292.39292.2 2.1413 0.3158 96.3 1333.1322 1332.549 6120.0 4 5.79 54.5 1 L.TPAS*VITSNPS*V.F YIL166C 1 2 1.8% 542 61898 8.9 U YIL166C SGDID:S000001428, Chr IX from 32566-30938, reverse complement, Uncharacterized ORF, "Hypothetical protein, member of the Dal5p subfamily of the major facilitator family" * 22_g_01_itms.34274.34274.3 2.3713 0.1377 99.0 1199.5743 1201.6105 2855.2 4 4.616 50.0 2 H.KGKSLFTEYE.E Reverse_YLR096W 1 1 1.7% 1147 128338 9.4 U KIN2 SGDID:S000004086, Chr XII from 332591-336034, Verified ORF, "Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p" * 22_g_01_itms.23319.23319.2 2.5021 0.2081 95.1 2230.132 2233.1003 8388.6 9 4.473 34.2 1 P.GTYPQAKLLEPAAFYLSGCF.T YGR094W 1 1 1.7% 1104 125770 7.0 U VAS1 SGDID:S000003326, Chr VII from 672190-675504, Verified ORF, "Mitochondrial and cytoplasmic valyl-tRNA synthetase" * 22_g_01_itms.39306.39306.3 2.3292 0.1716 91.7 2022.5643 2023.1075 3933.8 15 3.869 33.3 1 K.TGEVIINPLKEDGSPKTPK.E YDL215C 1 1 1.7% 1092 124332 5.7 U GDH2 SGDID:S000002374, Chr IV from 73919-70641, reverse complement, Verified ORF, "NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels" * 22_g_01_itms.07088.07088.3 3.0881 0.1111 91.8 1975.5243 1971.951 5160.5 193 3.874 33.3 1 P.NDDPAGVDISSQDLLKGDI.E YMR273C 1 1 1.7% 915 103358 6.4 U ZDS1 SGDID:S000004886, Chr XIII from 813979-811232, reverse complement, Verified ORF, "Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; has a role in localization of Bcy1p, a regulatory subunit of protein kinase A; implicated in mRNA nuclear export" * 22_g_01_itms.33180.33180.3 2.1093 0.0601 93.4 1717.3143 1713.8845 5469.6 205 3.969 35.0 1 A.ITLARTLSMAGSYSDK.K YLR258W 1 1 1.7% 705 80079 6.4 U GSY2 SGDID:S000004248, Chr XII from 660718-662835, Verified ORF, "Glycogen synthase, similar to Gsy1p; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase" * 22_g_01_itms.39729.39729.2 1.6754 0.101 90.6 1321.0922 1320.7163 5827.3 14 4.303 59.1 1 K.SKAPITVAQYKD.H Reverse_YIL026C 1 1 1.6% 1150 133009 5.1 U IRR1 SGDID:S000001288, Chr IX from 307927-304475, reverse complement, Verified ORF, "Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability" * 22_g_01_itms.33302.33302.3 2.3064 0.028 92.8 2063.9343 2067.8604 6063.1 396 3.951 30.9 1 D.PDNNETNAHDTERESNPQ.L Reverse_YGL216W 1 1 1.6% 805 91090 7.1 U KIP3 SGDID:S000003184, Chr VII from 84884-87301, Verified ORF, "Kinesin-related motor protein involved in mitotic spindle positioning " * 22_g_01_itms.36534.36534.2 1.662 0.2211 95.3 1207.9321 1205.5438 3054.0 249 4.532 41.7 1 S.DGLGPFNAEAGSA.E Reverse_YLR399C 1 1 1.6% 686 76978 6.0 U BDF1 SGDID:S000004391, Chr XII from 921596-919536, reverse complement, Verified ORF, "Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p" * 22_g_01_itms.37828.37828.2 1.5582 0.1061 91.2 1281.8522 1283.5981 5476.3 5 4.329 50.0 1 D.FYTPLNMSVPD.V YIL173W 1 1 1.5% 1549 174427 5.3 U VTH1 SGDID:S000001435, Chr IX from 11492-16141, Verified ORF, "Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting" YJL222W 1 1 1.5% 1549 174401 5.3 U VTH2 SGDID:S000003758, Chr X from 11475-16124, Verified ORF, "Putative membrane glycoprotein with strong similarity to Vth1p and Pep1p/Vps10p, may be involved in vacuolar protein sorting" 22_g_01_itms.17451.17451.2 2.5706 0.3056 99.6 2528.4321 2526.4329 9213.2 305 5.188 22.7 1 Y.NLIALSDICDKSKGKSVLVKPLQ.L Reverse_YDR217C 1 1 1.5% 1309 148397 5.3 U RAD9 SGDID:S000002625, Chr IV from 903474-899545, reverse complement, Verified ORF, "DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate" * 22_g_01_itms.46015.46015.3 2.2642 0.1895 96.2 2033.5743 2037.0657 5146.5 409 4.175 26.4 1 E.LGVVVYENGDFTVADGIRI.D Reverse_YDL195W 1 1 1.5% 1273 138703 5.7 U SEC31 SGDID:S000002354, Chr IV from 107209-111030, Verified ORF, "Essential phosphoprotein component (p150) of the COPII coat of secretory pathway vesicles, in complex with Sec13p; required for ER-derived transport vesicle formation" * 22_g_01_itms.38326.38326.3 2.4456 0.1046 94.3 1889.2144 1886.0387 8724.3 1 4.067 36.1 1 A.NAPIGNLPTPTSLINPPAV.S Reverse_YGR170W 1 1 1.5% 1138 130065 7.9 U PSD2 SGDID:S000003402, Chr VII from 837147-840563, Verified ORF, "Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine" * 22_g_01_itms.39222.39222.3 2.6933 0.1593 93.9 2047.8544 2049.147 6296.5 30 4.039 34.4 1 S.KNHKGRQLHLLRSGIYE.K Reverse_YKR086W 1 1 1.5% 1071 121652 8.2 U PRP16 SGDID:S000001794, Chr XI from 599499-602714, Verified ORF, "RNA helicase in the DEAH-box family involved in the second catalytic step of splicing, exhibits ATP-dependent RNA unwinding activity" * 22_g_01_itms.27686.27686.3 2.5189 0.1231 96.0 1802.7843 1803.0526 7754.0 10 4.166 40.0 1 F.KKANMTASTIILKLDR.R YLR095C 1 1 1.5% 812 93446 5.8 U IOC2 SGDID:S000004085, Chr XII from 332116-329678, reverse complement, Verified ORF, "Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif" * 22_g_01_itms.08195.08195.2 2.2185 0.147 95.3 1213.8522 1212.6112 4072.2 326 4.476 50.0 1 G.TVGPLQTGDVPE.L YLR337C 1 1 1.5% 817 82594 9.9 U VRP1 SGDID:S000004329, Chr XII from 805106-802653, reverse complement, Verified ORF, "Proline-rich, actin-associated protein involved in cytoskeletal organization and cytokinesis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP)" * 22_g_01_itms.08415.08415.3 2.0676 0.1114 91.8 1217.6643 1215.6373 5472.0 63 3.873 36.4 1 K.NPTKSPPPPPSP.S YKL029C 1 2 1.5% 669 74376 8.2 U MAE1 SGDID:S000001512, Chr XI from 384368-382359, reverse complement, Verified ORF, "Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids" * 22_g_01_itms.09523.09523.2 3.0109 0.0908 95.3 1342.1122 1339.5992 8259.9 14 4.474 72.2 2 R.DFDECLQWVK.A YNR016C 3 4 1.4% 2233 250351 6.3 U ACC1 SGDID:S000005299, Chr XIV from 661376-654675, reverse complement, Verified ORF, "Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA; required for de novo biosynthesis of long-chain fatty acids" * 22_g_01_itms.08801.08801.2 4.6689 0.4208 100.0 2060.0122 2059.9702 8409.6 1 7.613 55.6 1 R.AVS*VSDLSYVANSQSSPLR.E * 22_g_01_itms.08814.08814.3 4.1877 0.2376 94.8 2061.4443 2059.9702 7984.2 10 4.844 36.1 1 R.AVS*VSDLSYVANSQSSPLR.E * 22_g_01_itms.09529.09529.2 2.9249 0.2984 99.3 1522.9922 1521.6749 7559.3 2 5.056 58.3 2 R.ETESGFEYGLFDK.G YER132C 1 1 1.4% 1753 195382 7.8 U PMD1 SGDID:S000000934, Chr V from 430445-425184, reverse complement, Verified ORF, "Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions" * 22_g_01_itms.21886.21886.2 2.5328 0.2867 96.8 2546.3523 2547.3586 8704.0 1 5.605 30.4 1 N.QISHLGSSASLPNS*PILPVLNIPL.P YJR041C 1 1 1.4% 1174 135117 6.4 U URB2 SGDID:S000003802, Chr X from 513677-510153, reverse complement, Verified ORF, "Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis" * 22_g_01_itms.33513.33513.3 2.6452 0.1655 96.0 2015.4543 2018.1367 6273.2 10 4.158 34.4 1 F.GYKSYLLLFNAIKEFLV.S Reverse_YLR335W 1 1 1.4% 720 77881 7.2 U NUP2 SGDID:S000004327, Chr XII from 797430-799592, Verified ORF, "Protein involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization" * 22_g_01_itms.12605.12605.2 1.4222 0.393 94.3 1142.5322 1141.4679 2968.2 179 5.389 38.9 1 N.QS*DAAHKTGF.T Reverse_YMR190C 1 1 1.3% 1447 163837 6.3 U SGS1 SGDID:S000004802, Chr XIII from 645257-640914, reverse complement, Verified ORF, "Nucleolar DNA helicase of the RecQ family involved in maintenance of genome integrity, regulates chromosome synapsis and meiotic crossing over; has similarity to human BLM and WRN helicases implicated in Bloom and Werner syndromes" * 22_g_01_itms.23583.23583.3 3.2225 0.1678 93.7 2313.5942 2314.2307 7931.6 50 4.025 33.3 1 L.NTRNFSQKLFVPEKLELNH.I Reverse_YMR280C 1 1 1.3% 1433 160485 9.0 U CAT8 SGDID:S000004893, Chr XIII from 831328-827027, reverse complement, Verified ORF, "Zinc cluster transcriptional activator necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements" * 22_g_01_itms.20992.20992.2 2.2745 0.0528 91.9 2107.3123 2110.2751 7080.4 295 4.371 29.4 1 L.FKDLREVKVNPAKLLLNI.S YDR080W 1 1 1.3% 992 113412 4.9 U VPS41 SGDID:S000002487, Chr IV from 604004-606982, Verified ORF, "Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport" * 22_g_01_itms.34183.34183.2 1.4191 0.0347 95.8 1321.2522 1322.5712 5819.0 264 4.569 41.7 1 A.SEPTASSKEEGSN.I YOR217W 1 1 1.3% 861 94903 9.2 U RFC1 SGDID:S000005743, Chr XV from 749301-751886, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" * 22_g_01_itms.17559.17559.3 2.2633 0.12 94.0 1306.1643 1308.651 4363.1 252 4.042 35.0 1 T.PLMIQENYLST.R Reverse_YDR422C 1 1 1.2% 863 96259 6.6 U SIP1 SGDID:S000002830, Chr IV from 1317907-1315316, reverse complement, Verified ORF, "Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions" * 22_g_01_itms.45464.45464.3 2.3362 0.1561 97.0 1201.5543 1202.7081 4308.8 79 4.225 50.0 1 S.RRSNSVSRIK.K YPR095C 1 1 1.1% 1226 137583 7.3 U SYT1 SGDID:S000006299, Chr XVI from 724715-721035, reverse complement, Verified ORF, "Guanine nucleotide exchange factor (GEF) for Arf proteins; involved in vesicular transport; suppressor of ypt3 mutations; member of the Sec7-domain family" * 22_g_01_itms.40159.40159.2 1.7946 0.0727 94.5 1343.9321 1341.6691 2005.1 23 4.456 45.8 1 F.KSGFSFVPGSTID.V Reverse_YDL223C 1 1 1.1% 1046 113616 6.4 U HBT1 SGDID:S000002382, Chr IV from 60406-57266, reverse complement, Verified ORF, "Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis" * 22_g_01_itms.39217.39217.2 1.6189 0.1049 90.3 1179.7722 1179.5282 3568.7 20 4.298 55.0 1 E.PNNTFDKADSA.I YDL140C 1 1 1.0% 1733 191610 5.6 U RPO21 SGDID:S000002299, Chr IV from 210562-205361, reverse complement, Verified ORF, "RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime" * 22_g_01_itms.09813.09813.3 2.7986 0.1827 93.4 1916.3944 1915.9772 8239.5 134 4.004 33.8 1 L.VSRGGCGNTQPTIRKDGL.K Reverse_YER008C 1 1 1.0% 1336 154694 5.4 U SEC3 SGDID:S000000810, Chr V from 171817-167807, reverse complement, Verified ORF, "Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion" * 22_g_01_itms.00530.00530.3 1.8426 0.0172 92.0 1291.3143 1290.6178 6108.3 139 3.898 33.3 1 E.GSLNSTNLGAIED.S YLR096W 1 1 1.0% 1147 128338 9.4 U KIN2 SGDID:S000004086, Chr XII from 332591-336034, Verified ORF, "Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p" * 22_g_01_itms.12523.12523.3 2.1163 0.1655 96.6 1401.3243 1403.7283 7073.1 119 4.216 43.2 1 P.KSHSRTISDYIP.S YHL035C 1 1 0.9% 1592 180925 8.5 U YHL035C SGDID:S000001027, Chr VIII from 32754-27976, reverse complement, Uncharacterized ORF, "Protein of unknown function, member of the ATP-binding cassette (ABC) family, potential Cdc28p substrate" * 22_g_01_itms.45519.45519.2 2.0562 0.223 99.3 1673.6522 1670.9553 8856.2 1 5.042 53.8 1 L.RVTKINIELTNRDV.T Reverse_YLR278C 1 1 0.8% 1341 151278 8.3 U YLR278C SGDID:S000004268, Chr XII from 704026-700001, reverse complement, Uncharacterized ORF, "Protein of unknown function, localizes to the nucleus; potential Cdc28p substrate" * 22_g_01_itms.31513.31513.2 1.373 0.1624 91.6 1063.9922 1062.4816 2347.1 196 4.335 45.0 1 D.NSNSLNAANAS.N YDR093W 1 1 0.7% 1612 182617 6.2 U DNF2 SGDID:S000002500, Chr IV from 631277-636115, Verified ORF, "Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the plasma membrane and late exocytic or early endocytic membranes, likely involved in protein transport; potential Cdc28p substrate" * 22_g_01_itms.32312.32312.2 1.9343 0.2944 100.0 1267.7122 1265.5935 3321.9 41 5.757 59.1 1 A.DMILLSTSDVDG.A YOR207C 1 1 0.7% 1149 129456 8.2 U RET1 SGDID:S000005733, Chr XV from 733457-730008, reverse complement, Verified ORF, "Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs" * 22_g_01_itms.46190.46190.2 1.3974 0.2579 96.2 1081.4321 1081.5325 11677.7 127 4.655 64.3 1 G.FGRCETRR.K YHR165C 1 1 0.4% 2413 279502 7.3 U PRP8 SGDID:S000001208, Chr VIII from 436950-429709, reverse complement, Verified ORF, "Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing" * 22_g_01_itms.16899.16899.2 1.4381 0.2145 95.3 837.33215 836.3824 3906.1 34 4.531 56.2 1 E.EAAGASTVM.K YLL040C 1 1 0.3% 3144 357850 5.6 U VPS13 SGDID:S000003963, Chr XII from 63644-54210, reverse complement, Verified ORF, "Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; homologous to human COH1; involved in sporulation, vacuolar protein sorting and protein-Golgi retention" * 22_g_01_itms.34156.34156.2 1.4357 0.2409 96.9 919.8522 921.54095 1588.0 89 4.753 57.1 1 N.TQIVVSFK.G YGL195W 1 1 0.3% 2672 296697 5.3 U GCN1 SGDID:S000003163, Chr VII from 131531-139549, Verified ORF, "Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA" * 22_g_01_itms.33256.33256.2 1.2322 0.1801 90.4 925.89215 925.47833 5974.3 53 4.283 56.2 1 L.QAAAYAAFI.Q Proteins Peptide IDs Spectra Unfiltered 10414 56894 62111 Filtered 350 529 1261 Forward matches 240 416 1145 Decoy matches 110 113 116 Forward FP rate 45.83 27.16 10.13 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0