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Protein Overview: MCM4

General Information:
Name(s) found: MCM4 / YPR019W [SGD]
Description(s) found:
Found 28 descriptions.
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  • Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt the DNA prior to replication; accumulates in the nucleus in G1; homolog of S. pombe Cdc21p [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 933 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    pre-replicative complex [IDALink to PubMed article]
    cytoplasm [IDALink to PubMed article][TASLink to PubMed article]
    MCM complex [IDALink to PubMed article]
    Biological Process: DNA replication initiation [TASLink to PubMed article]
    pre-replicative complex assembly [IPILink to PubMed article]
    DNA unwinding involved in replication [TASLink to PubMed article]
    Molecular Function: chromatin binding [TASLink to PubMed article]
    ATP-dependent DNA helicase activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSQQSSSPTK EDNNSSSPVV PNPDSVPPQL SSPALFYSSS SSQGDIYGRN NSQNLSQGEG  60
   61 NIRAAIGSSP LNFPSSSQRQ NSDVFQSQGR QGRIRSSASA SGRSRYHSDL RSDRALPTSS 120
  121 SSLGRNGQNR VHMRRNDIHT SDLSSPRRIV DFDTRSGVNT LDTSSSSAPP SEASEPLRII 180
  181 WGTNVSIQEC TTNFRNFLMS FKYKFRKILD EREEFINNTT DEELYYIKQL NEMRELGTSN 240
  241 LNLDARNLLA YKQTEDLYHQ LLNYPQEVIS IMDQTIKDCM VSLIVDNNLD YDLDEIETKF 300
  301 YKVRPYNVGS CKGMRELNPN DIDKLINLKG LVLRSTPVIP DMKVAFFKCN VCDHTMAVEI 360
  361 DRGVIQEPAR CERIDCNEPN SMSLIHNRCS FADKQVIKLQ ETPDFVPDGQ TPHSISLCVY 420
  421 DELVDSCRAG DRIEVTGTFR SIPIRANSRQ RVLKSLYKTY VDVVHVKKVS DKRLDVDTST 480
  481 IEQELMQNKV DHNEVEEVRQ ITDQDLAKIR EVAAREDLYS LLARSIAPSI YELEDVKKGI 540
  541 LLQLFGGTNK TFTKGGRYRG DINILLCGDP STSKSQILQY VHKITPRGVY TSGKGSSAVG 600
  601 LTAYITRDVD TKQLVLESGA LVLSDGGVCC IDEFDKMSDS TRSVLHEVME QQTISIAKAG 660
  661 IITTLNARSS ILASANPIGS RYNPNLPVTE NIDLPPPLLS RFDLVYLVLD KVDEKNDREL 720
  721 AKHLTNLYLE DKPEHISQDD VLPVEFLTMY ISYAKEHIHP IITEAAKTEL VRAYVGMRKM 780
  781 GDDSRSDEKR ITATTRQLES MIRLAEAHAK MKLKNVVELE DVQEAVRLIR SAIKDYATDP 840
  841 KTGKIDMNLV QTGKSVIQRK LQEDLSREIM NVLKDQASDS MSFNELIKQI NEHSQDRVES 900
  901 SDIQEALSRL QQEDKVIVLG EGVRRSVRLN NRV

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details (MIPS) Mewes HW, et al. (2004) CDC45 CDC6 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32 SLD3
    View Details Ho Y, et al. (2002) ADR1 ARC15 ARC18 ARC19 ARC35 ARC40 ARP2 ARP3 BNI1 DUR1,2 IMH1 KAP122 MCM4 MCM7 MET18 RPA135 RPT4 SAN1 SRP1 TRP3 UBI4 YJR029W YPL113C
    View Details Qiu et al. (2008) ABF1 CDC45 CDC6 DPB11 DPB2 DPB3 DPB4 HCS1 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 MRC1 NOC3 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL1 POL2 POL3 POL30 POL31 POL32 RFA2 RFC1 RFC4 RFC5 RRM3 SLD3

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data MCM4 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..181] MSA 2.065996 View MSA. No confident structure predictions are available.
    2 View Details [182..306] PSI-BLAST 176.9691 DNA replication initiator (cdc21/cdc54) N-terminal domain
    3 View Details [307..340]
    [393..526]    		 PSI-BLAST 176.9691 DNA replication initiator (cdc21/cdc54) N-terminal domain
    4 View Details [341..392] PSI-BLAST 176.9691 DNA replication initiator (cdc21/cdc54) N-terminal domain
    5 View Details [527..755] PSI-BLAST 50.0 ATPase subunit of magnesium chelatase, BchI
    6 View Details [756..842] PSI-BLAST 50.0 ATPase subunit of magnesium chelatase, BchI
    7 View Details [843..933] deduced N/A Confident ab initio structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • telomeric DNA binding
    		•  4.81350293592083 bayes_pls_golite062009
  • telomerase inhibitor activity
    		•  4.48082903165152 bayes_pls_golite062009
  • single-stranded telomeric DNA binding
    		•  4.34033743483519 bayes_pls_golite062009
  • nucleic acid binding
    		•  4.29626483284963 bayes_pls_golite062009
  • DNA binding
    		•  3.71771169141041 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  3.51998515668903 bayes_pls_golite062009
  • structural molecule activity
    		•  3.29857127235306 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  3.06066809614422 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  3.0183551365402 bayes_pls_golite062009
  • binding
    		•  2.83465364476231 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.10110937001387 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  1.9581702424972 bayes_pls_golite062009
  • DNA replication origin binding
    		•  1.84819558112187 bayes_pls_golite062009
  • translation regulator activity
    		•  1.46320772716954 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  1.40718318783582 bayes_pls_golite062009
  • transcription repressor activity
    		•  1.30343463892105 bayes_pls_golite062009
  • translation initiation factor activity
    		•  1.27539679024898 bayes_pls_golite062009
  • transcription activator activity
    		•  1.24141936405635 bayes_pls_golite062009
  • protein binding
    		•  1.20309946451799 bayes_pls_golite062009
  • DNA-directed RNA polymerase activity
    		•  1.19766815287926 bayes_pls_golite062009
  • RNA polymerase activity
    		•  1.19766815287926 bayes_pls_golite062009
  • ribonuclease activity
    		•  1.19236946227657 bayes_pls_golite062009
  • transcription factor activity
    		•  1.14242112812892 bayes_pls_golite062009
  • DNA helicase activity
    		•  1.12203697857261 bayes_pls_golite062009
  • DNA-dependent ATPase activity
    		•  0.638831894247026 bayes_pls_golite062009
  • nuclease activity
    		•  0.57381531077345 bayes_pls_golite062009
  • aminoacyl-tRNA ligase activity
    		•  0.474251377131502 bayes_pls_golite062009
  • ligase activity, forming carbon-oxygen bonds
    		•  0.47336621289748 bayes_pls_golite062009
  • ligase activity, forming aminoacyl-tRNA and related compounds
    		•  0.47336621289748 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  0.325774164899685 bayes_pls_golite062009
  • four-way junction helicase activity
    		•  0.31769813397725 bayes_pls_golite062009
  • exonuclease activity
    		•  0.28313884527829 bayes_pls_golite062009
  • RNA binding
    		•  0.18502033889261 bayes_pls_golite062009
  • exoribonuclease activity, producing 5'-phosphomonoesters
    		•  0.125256208482441 bayes_pls_golite062009
  • exoribonuclease activity
    		•  0.109774918833391 bayes_pls_golite062009
  • exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  0.0954216620147701 bayes_pls_golite062009
  • DEAD/H-box RNA helicase binding
    		•  0.0627405398613412 bayes_pls_golite062009
  • ATP-dependent DNA helicase activity
    		•  0.0179535526984695 bayes_pls_golite062009
  • helicase activity
    		•  0.0121541775159524 bayes_pls_golite062009
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.
    7 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle