Protein Overview: MYO4

General Information:
Name(s) found:	MYO4 / YAL029C [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	One of two type V myosin motors (along with MYO2) involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1471 amino acids
Gene Ontology:
Cellular Component:	cellular bud [IDA] mitochondrion [IDA ] myosin V complex [ISS ] filamentous actin [IDA]
Biological Process:	intracellular mRNA localization [IMP ][IPI ] endoplasmic reticulum inheritance [IMP] gene conversion at mating-type locus [IMP]
Molecular Function:	actin filament binding [IDA] microfilament motor activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFEVGTKCW YPHKEQGWIG GEVTKNDFFE GTFHLELKLE DGETVSIETN SFENDDDHPT  60
   61 LPVLRNPPIL ESTDDLTTLS YLNEPAVLHA IKKRYMNGQI YTYSGIVLIA ANPFDKVDHL 120
  121 YSREMIQNYS SKRKDELEPH LFAIAEEAYR FMVHEKANQT VVVSGESGAG KTVSAKYIMR 180
  181 YFASVQESNN REGEVEMSQI ESQILATNPI MEAFGNAKTT RNDNSSRFGK YLQILFDENT 240
  241 TIRGSKIRTY LLEKSRLVYQ PETERNYHIF YQILEGLPEP VKQELHLSSP KDYHYTNQGG 300
  301 QPNIAGIDEA REYKITTDAL SLVGINHETQ LGIFKILAGL LHIGNIEMKM TRNDASLSSE 360
  361 EQNLQIACEL LGIDPFNFAK WIVKKQIVTR SEKIVTNLNY NQALIARDSV AKFIYSTLFD 420
  421 WLVDNINKTL YDPELDQQDH VFSFIGILDI YGFEHFEKNS FEQFCINYAN EKLQQEFNQH 480
  481 VFKLEQEEYV KEEIEWSFIE FSDNQPCIDL IENKLGILSL LDEESRLPSG SDESWASKLY 540
  541 SAFNKPPSNE VFSKPRFGQT KFIVSHYAVD VEYEVEGFIE KNRDSVSLGH LDVFKATTNP 600
  601 IFKQILDNRE LRSDDAPEEQ NTEKKIMIPA RLSQKKPTLG SMFKKSLGEL MAIINSTNVH 660
  661 YIRCIKPNSE KKPWEFDNLM VLSQLRACGV LETIRISCAG FPSRWTFDEF VQRYFLLTDY 720
  721 SLWSGILYNP DLPKEAIVNF CQSILDATIS DSAKYQIGNT KIFFKAGMLA FLEKLRTNKM 780
  781 NEICIIIQKK IRARYYRLQY LQTMESIKKC QSQIRSLLVR TRVDHELKTR AAILLQTNIR 840
  841 ALWKREYYRA AIGQIIKLQC TCKRKLILDS VNRKFMLMAA VIIQSYIRSY GHKTDYRTLK 900
  901 RSSILVQSAM RMQLARRRYI VLQKEVEERN IRASYGIGLL EEAIEFKNSF ILNLEMLNDS 960
  961 YTRLTQLLQG DLSNIPSKQR QEYETIVNGY NDKISKLKTL QVEIMNTLNK KNALKERKKK1020
 1021 QSSLIQSHMQ SLAAIKGNKP SRLSDEVKSM KQELAFIENV IAQDFTTTYS ANKNDKVKGL1080
 1081 GIAGQQVKPK LVNVIRRESG NPDLLELLMD LNCYTLEVTE GYLKKVNVTE VNGDNVLGPI1140
 1141 HVITTVVSSL VRNGLLIQSS KFISKVLLTV ESIVMSLPKD ETMLGGIFWL SNLSRLPAFA1200
 1201 ANQKTLYEAN GGDEKDKLTL IYLNDLENET LKVFDKIYST WLVKFMKHAS AHIEIFDMVL1260
 1261 NEKLFKNSGD EKFAKLFTFL NEFDAVLCKF QVVDSMHTKI FNDTLKYLNV MLFNDLITKC1320
 1321 PALNWKYGYE VDRNIERLVS WFEPRIEDVR PNLIQIIQAV KILQLKISNL NEFKLLFDFW1380
 1381 YALNPAQIQA ILLKYKPANK GEAGVPNEIL NYLANVIKRE NLSLPGKMEI MLSAQFDSAK1440
 1441 NHLRYDTSAI TQNSNTEGLA TVSKIIKLDR K

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CMD1 MLC1 MYO2 MYO4 SHE2 SHE3
View Details	(MIPS) Mewes HW, et al. (2004)		MYO1 MYO2 MYO3 MYO4 MYO5 SMY1 SMY2
View Details	Krogan NJ, et al. (2006)		ATG33 BUB1 CUL3 GUT1 ISM1 MEC3 MLC1 MYO2 MYO4 RGA1 RSB1 SAT4 SHE2 SHE3 SHE4 SRC1 SSH4 STT4 TOF2 TVP18 YDR186C YOL098C
View Details	Gavin AC, et al. (2006)		MLC1 MYO4 SHE2 SHE3
View Details	Gavin AC, et al. (2002)		ACT1 ADA2 ADH1 ARX1 GCN1 GCN5 HEM13 HFI1 HSC82 KAP123 MES1 MLC1 MLC2 MYO1 MYO2 MYO4 NGG1 NIP1 PSK1 PTC1 RPB2 RPO21 SGF29 SGF73 SHE2 SHE3 SPT20 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF3 TAF5 TAF6 TAF7 TAF9 UBP8 VAS1 YEF3
View Details	Ho Y, et al. (2002)		AAC3 BZZ1 CDC25 CKI1 CMD1 CMK2 CMP2 COF1 CPR1 EDE1 GCR2 GIN4 HCH1 HOM3 HUL5 HXT6 HYP2 ILS1 IPP1 IRA1 IRA2 LAS17 MLC1 MYO2 MYO3 MYO4 MYO5 NAP1 PEP1 PGM2 PMD1 PRS2 PRS3 PRS5 PST2 RIM11 RPN1 RVS167 SHE3 SHE4 SLA1 SOD1 SPC110 SUL2 SUP45 TPS1 TSL1 UBA1 VAS1 VPS13 VRP1 YDR170W-A YER138C YER160C YJR027W YJR028W YNK1 YNR065C
View Details	Qiu et al. (2008)		ABP140 BNI1 BNR1 MYO1 MYO2 MYO3 MYO4 MYO5 PFY1 SHE3 SMY1 TPM1 TPM2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD51	MYO4	1	Unpublished Fields Lab Data
View Screen	RAD6	MYO4	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MYO4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..64]	PSI-BLAST	2640.0	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
2	View Details	[65..341] [416..453] [649..693]	PSI-BLAST	2640.0	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
3	View Details	[342..415] [454..484] [499..648] [694..697]	PSI-BLAST	2640.0	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
4	View Details	[485..498] [698..779]	PSI-BLAST	2640.0	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
5	View Details	[780..849]	PSI-BLAST	10.0	SCALLOP MYOSIN REGULATORY DOMAIN
6	View Details	[850..914]	PSI-BLAST	7.30103	Tropomyosin
7	View Details	[915..996]	PSI-BLAST	7.30103	Tropomyosin
8	View Details	[997..1056]	PSI-BLAST	7.30103	Tropomyosin
9	View Details	[1057..1134]	PSI-BLAST	7.30103	Tropomyosin
10	View Details	[1135..1231] [1405..1471]	ORFEUS	7.17	p120GAP domain
11	View Details	[1232..1404]	ORFEUS	7.17	p120GAP domain

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2