Protein Overview: Csk-PH

General Information:
Name(s) found:	Csk-PH / FBpp0289773 [FlyBase]
Description(s) found: SHOW ONLY BEST	gi\|45550738\|ref\|NP_650097.2\| C-terminal Src kinase, isoform H [Drosophila melanogaster] [NCBI NR] gi\|45446455\|gb\|AAF54668.3\| C-terminal Src kinase, isoform H [Drosophila melanogaster] [NCBI NR] The gene C-terminal Src kinase is referred to in FlyBase by the symbol Dmel\Csk (CG42317, FBgn0259217). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is 3R:7458992..7476418. It has the cytological map location 86E13. There is experimental evidence that it has the molecular function</span>: protein tyrosine kinase activity. There is experimental evidence that it is involved in the biological process</span>: negative regulation of growth; adherens junction maintenance; positive regulation of programmed cell death; establishment or maintenance of epithelial cell apical/basal polarity; epithelial structure maintenance; negative regulation of cell proliferation; germarium-derived egg chamber formation. 27 alleles are reported. The phenotypes of these alleles are annotated with: eye; dorsal mesothoracic disc; anterior crossvein; wing vein L4; wing; ommatidium. It has 6 annotated transcripts and 6 annotated polypeptides. [FlyBase] (AAF54668) CG17309-PA [DrosMelano7227.FASTAC.txt] CG17309-PA, isoform A [Drosophila melanogaster]gi\|45550738\|ref\|NP_650097.2\| CG17309-PA [Drosophila melanogaster] [nrdb_11152004_Chlamydomonas] type=protein; loc=3R:complement(7460542..7460718,7460780..7460866,7460938..7461133,7461191..7461352,7461423..7461691,7461747..7461966,7462024..7462727,7462792..7463336,7472510..7472567); ID=FBpp0289773; name=Csk-PH; parent=FBgn0259217,FBtr0300546; dbxref= [fly-100309-contam.fasta] CG17309-PA, isoform A [Drosophila melanogaster] [nr-271106-contam.fasta] C-terminal Src kinase CG17309-PA, isoform A [Drosophila melanogaster] [nr-271106-contam.fasta] type=protein; loc=3R:complement(7460542..7460718,7460780..7460866,7460938..7461133,7461191..7461352,7461423..7461691,7461747..7461966,7462024..7462727,7462792..7463336,7472510..7472567); ID=FBpp0289773; name=Csk-PH; parent=FBgn0259217,FBtr0300546; dbxref=FlyBase:FBpp0289773,FlyBase_Annotation_IDs:CG42317-PH,REFSEQ:NP_650097,GB_protein:AAF54668; MD5=06b8b096b2e7738304befc65c6aecb9d; length=805; release=r5.16; species=Dmel; [fly-contam-His-TEV-Tub4complex.fasta] type=protein; loc=3R:complement(7460542..7460718,7460780..7460866,7460938..7461133,7461191..7461352,7461423..7461691,7461747..7461966,7462024..7462727,7462792..7463336,7472510..7472567); ID=FBpp0289773; name=Csk-PH; parent=FBgn0259217,FBtr0300546; dbxref=FlyBase:FBpp0289773,FlyBase_Annotation_IDs:CG42317-PH,REFSEQ:NP_650097,GB_protein:AAF54668; MD5=06b8b096b2e7738304befc65c6aecb9d; length=805; release=r5.21; species=Dmel; [Other_drosophila_na_09-09-2009_reversed.fasta] C-terminal Src kinase, isoform H OS=Drosophila melanogaster GN=Csk PE=2 SV=3 [UniProt_drosophila_07-01-2011_reversed.fasta] type=protein; loc=3R:complement(7460542..7460718,7460780..7460866,7460938..7461133,7461191..7461352,7461423..7461691,7461747..7461966,7462024..7462727,7462792..7463336,7472510..7472567); ID=FBpp0289773; name=Csk-PH; parent=FBgn0262081,FBtr0300546; dbxref=FlyBase:FBpp0289773,FlyBase_Annotation_IDs:CG42317-PH,REFSEQ:NP_650097,GB_protein:AAF54668,FlyMine:FBpp0289773,modMine:FBpp0289773; MD5=06b8b096b2e7738304befc65c6aecb9d; length=805; release=r5.46; species=Dmel; [fly-062812-sfps-sandra-contam.fasta] type=protein; loc=3R:complement(join(11646788..11646845,11637070..11637614,11636302..11637005,11636025..11636244,11635701..11635969,11635469..11635630,11635216..11635411,11635058..11635144,11634820..11634996)); ID=FBpp0289773; name=Csk-PH; parent=FBgn0262081,FBtr0300546; dbxref=FlyBase:FBpp0289773,FlyBase_Annotation_IDs:CG42317-PH,REFSEQ:NP_650097,GB_protein:AAF54668,FlyMine:FBpp0289773,modMine:FBpp0289773; MD5=06b8b096b2e7738304befc65c6aecb9d; length=805; release=r6.02; species=Dmel; [fly-2014-05-contam.fasta] type=protein; loc=3R:complement(join(11646788..11646845,11637070..11637614,11636302..11637005,11636025..11636244,11635701..11635969,11635469..11635630,11635216..11635411,11635058..11635144,11634820..11634996)); ID=FBpp0289773; name=Csk-PH; parent=FBgn0262081,FBtr0300546; dbxref=FlyBase:FBpp0289773,FlyBase_Annotation_IDs:CG42317-PH,REFSEQ:NP_650097,GB_protein:AAF54668,FlyMine:FBpp0289773,modMine:FBpp0289773; MD5=06b8b096b2e7738304befc65c6aecb9d; length=805; release=r6.07; species=Dmel; [fly-08202015-contam.fasta] Isoform of Q8INJ8, Tyrosine-protein kinase OS=Drosophila melanogaster GN=Csk PE=1 SV=3 [DROME.fasta.20160308] FBpp0289773 type=protein; loc=3R:complement(join(11646788..11646845,11637070..11637614,11636302..11637005,11636025..11636244,11635701..11635969,11635469..11635630,11635216..11635411,11635058..11635144,11634820..11634996)); ID=FBpp0289773; name=Csk-PH; parent=FBgn0262081,FBtr0300546; dbxref=FlyBase:FBpp0289773,FlyBase_Annotation_IDs:CG42317-PH,REFSEQ:NP_650097,GB_protein:AAF54668,FlyMine:FBpp0289773,modMine:FBpp0289773; MD5=06b8b096b2e7738304befc65c6aecb9d; length=805; release=r6.09; species=Dmel; [dmel-all-translation-r6.09.fasta] [fly-122215-dmel-6-09-contam.fasta] type=protein; loc=3R:complement(join(11646788..11646845,11637070..11637614,11636302..11637005,11636025..11636244,11635701..11635969,11635469..11635630,11635216..11635411,11635058..11635144,11634820..11634996)); ID=FBpp0289773; name=Csk-PH; parent=FBgn0262081,FBtr0300546; dbxref=FlyBase:FBpp0289773,FlyBase_Annotation_IDs:CG42317-PH,REFSEQ:NP_650097,GB_protein:AAF54668,UniProt/TrEMBL:Q9VGK8,FlyMine:FBpp0289773,modMine:FBpp0289773; MD5=06b8b096b2e7738304befc65c6aecb9d; length=805; release=r6.14; species=Dmel; [fly-dmel-jan2017-sandracontam.fasta]
Organism:	Drosophila melanogaster
Length:	805 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IEA]
Biological Process:	negative regulation of growth [IGI][IMP] germarium-derived egg chamber formation [IMP] intracellular signaling pathway [IEA] epithelial structure maintenance [IMP] negative regulation of cell proliferation [IMP] establishment or maintenance of epithelial cell apical/basal polarity [IMP] positive regulation of programmed cell death [IMP] adherens junction maintenance [IMP] protein amino acid phosphorylation [IEA][NAS]
Molecular Function:	ATP binding [IEA] protein tyrosine kinase activity [IDA][ISS][NAS] protein binding [IEA] non-membrane spanning protein tyrosine kinase activity [IEA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSESIAIGFL QYIITINYDD EEAQARKTSH EKSQKPKPKP EENHKEKEAI SRKASGEGDL  60
   61 ITVEIKQGLL RKQTATTTTG QAMNSHATAT QNGQTTVVGH HRGFSQPSVT VAAAAATGST 120
  121 AGDPWFLQTT GHQMPPTAPL RHNKRPAPQP NAVLGAGSSG TAPGSAVGAV LLHQQQLSQS 180
  181 QPHIVPPSIP PHHHHHRENN VHPIQAGAAA ALHLSSHALN SHNLISSASN HSPKSPSQTQ 240
  241 QQQQTTNQQQ QQQQQQASHS ILSTFAPAPT VAAVQPAGSQ SVAGTAAAVA AVAQQQQQQH 300
  301 LQLLANCPPG GAGLMSSSLN AAQMGMHGAE RALPPKSLAL SGSQHGSNML LHTAPQPPQA 360
  361 SQPAAVVGVS GSGSAGMSTS LHSFDINGVT AGSGVNASSA WSSGSTTAMN HASLSPTALA 420
  421 PQQGRSRCEV KLNAMPWFHG SITRDEAEHL LQPREDGLFL VRESTNFPGD YTLCVCFQSK 480
  481 VEHYRVKYLE NKLTIDDEEY FENLGQLVAH YEADADGLCT QLIKCLPKLG KQEFCINSKD 540
  541 FVDKGWVIPE AELQLRESIG KGEFGDVMLG ILRNEKVAVK MLKDEGAVQK FLAEASVMTT 600
  601 LEHDNLVKFI GLVFTSKHLY LVTEYMSKGS LVDYLRSRGR QHITKKDQII FAYDTASGME 660
  661 YLEAKKVVHR DLAARNVLIS EDCVAKVSDF GLAREECYNL DVGKLPIKWT APEALKNGRF 720
  721 SNKSDMWSFG ILLWEIYSFG RVPYPRIPLA DVVKHVEVGY KMEAPEGCPP EIYEMMRQAW 780
  781 DLNPAKRPTF AELKVKLQLL NNATA

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
cytoskeletal protein binding	4.41722924924158	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
actin binding	3.91045208670052	bayes_pls_golite062009
phosphoinositide 3-kinase binding	3.43994026029707	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase activity	3.41480681854114	bayes_pls_golite062009
transmembrane receptor protein kinase activity	3.40140621607334	bayes_pls_golite062009
CD8 receptor binding	3.34636716492159	bayes_pls_golite062009
protein binding	3.13677730481089	bayes_pls_golite062009
binding	3.06905798270146	bayes_pls_golite062009
protein tyrosine kinase activity	2.8153155526437	bayes_pls_golite062009
actin filament binding	2.72908433443928	bayes_pls_golite062009
ion transmembrane transporter activity	2.65451266834284	bayes_pls_golite062009
transmembrane receptor activity	2.59526003830791	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.4913932444423	bayes_pls_golite062009
cation transmembrane transporter activity	2.46424348876434	bayes_pls_golite062009
receptor activity	2.39311927684508	bayes_pls_golite062009
SH2 domain binding	2.08937039830657	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
metal ion transmembrane transporter activity	1.9016235206634	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.81249386965144	bayes_pls_golite062009
non-membrane spanning protein tyrosine kinase activity	1.66669268613198	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
vinculin binding	1.63216315345235	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
PTB domain binding	1.56656184134104	bayes_pls_golite062009
growth factor binding	1.53863828216563	bayes_pls_golite062009
MAP kinase kinase activity	1.42275431028856	bayes_pls_golite062009
molecular transducer activity	1.34867629228264	bayes_pls_golite062009
signal transducer activity	1.34867629228264	bayes_pls_golite062009
transporter activity	1.30447542854757	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
tubulin binding	1.13686658158825	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase activity	1.10045286406485	bayes_pls_golite062009
transforming growth factor beta receptor activity	1.10045286406485	bayes_pls_golite062009
transmembrane transporter activity	1.08558010881058	bayes_pls_golite062009
cation channel activity	1.04999722123563	bayes_pls_golite062009
structural molecule activity	0.989403012920596	bayes_pls_golite062009
substrate-specific transporter activity	0.820987609295422	bayes_pls_golite062009
MAP kinase activity	0.81943579965027	bayes_pls_golite062009
epidermal growth factor receptor binding	0.80673395326897	bayes_pls_golite062009
fibroblast growth factor receptor activity	0.75189004129952	bayes_pls_golite062009
microtubule binding	0.734274677384472	bayes_pls_golite062009
JUN kinase kinase kinase kinase activity	0.546647574638943	bayes_pls_golite062009
insulin binding	0.546269851452869	bayes_pls_golite062009
structural constituent of muscle	0.52430033936893	bayes_pls_golite062009
activin binding	0.510364757301021	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.499492353295369	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
protein kinase C activity	0.41587987561486	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
3-phosphoinositide-dependent protein kinase binding	0.372842462336059	bayes_pls_golite062009
structural constituent of cytoskeleton	0.371928447183203	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
passive transmembrane transporter activity	0.287189255116606	bayes_pls_golite062009
channel activity	0.287189255116606	bayes_pls_golite062009
insulin receptor binding	0.275913005279959	bayes_pls_golite062009
kinase binding	0.26752427107238	bayes_pls_golite062009
substrate-specific channel activity	0.261869836686301	bayes_pls_golite062009
ion channel activity	0.241519346930618	bayes_pls_golite062009
insulin receptor activity	0.188254408239569	bayes_pls_golite062009
cell adhesion molecule binding	0.150740450460731	bayes_pls_golite062009
receptor binding	0.0742205785215131	bayes_pls_golite062009
JUN kinase kinase kinase activity	0.0613131034808199	bayes_pls_golite062009
transforming growth factor beta receptor activity, type I	0.0506751594607393	bayes_pls_golite062009
cation binding	0.046173055856133	bayes_pls_golite062009
metal ion binding	0.046173055856133	bayes_pls_golite062009
ion binding	0.0389662058697694	bayes_pls_golite062009
protein kinase binding	0.00674998152095019	bayes_pls_golite062009

Source: Reynolds et al. 2008. Manuscript submitted

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..189]	deduced	N/A	No confident structure predictions are available.
2	View Details	[190..342]	deduced	N/A	No confident structure predictions are available.
3	View Details	[343..805]	PSI-BLAST	102.0	Carboxyl-terminal src kinase (csk)

Protein Overview: Csk-PH

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: