Protein Overview: CHL1

General Information:
Name(s) found:	CHL1 / YPL008W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|965085\|gb\|AAB68097.1\| Chl1p [NCBI NR] pir\|\|S12499 CHL1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|683782\|emb\|CAA88378.1\| Chl1p [Saccharomyces cerevisiae] [NCBI NR] gi\|6325249\|ref\|NP_015317.1\| Conserved nuclear protein required to establish sister-chromatid pairing during S-phase, probable DNA helicase with similarity to human BACH1, which associates with tumor suppressor BRCA1; associates with acetyltransferase Ctf7 [NCBI NR] gi\|3538\|emb\|CAA39922.1\| CHL1 [Saccharomyces cerevisiae] [NCBI NR] gi\|1314068\|emb\|CAA95033.1\| Chl1p [Saccharomyces cerevisiae] [NCBI NR] gi\|116351\|sp\|P22516.1\|CHL1_YEAST RecName: Full=ATP-dependent RNA helicase CHL1; AltName: Full=Chromosome loss protein 1; AltName: Full=Chromosome transmission fidelity protein 1 [NCBI NR] ATP-dependent RNA helicase CHL1 OS=Saccharomyces cerevisiae GN=CHL1 PE=1 SV=1 [Swiss-Prot] CHL1 Protein of the DEAH box family [mips] Conserved nuclear protein required to establish sister-chromatid pairing during S-phase, probable DNA helicase with similarity to human BACH1, which associates with tumor suppressor BRCA1; associates with acetyltransferase Ctf7p [SGD] (P22516) CHL1 protein [4932.FASTACCERV.txt] Chr XVI from 539380-541965 [hmr_yeast5_DM3-1_pombe.fasta] CHL1, Chr XVI from 539380-541965 [yeast_orfs2] CHL1 SGDID:S0005929, Chr XVI from 539380-541965, Verified ORF [yeastflysequest.fasta] CHL1 SGDID:S0005929, Chr XVI from 539382-541967, Verified ORF [10112005cervSGD.fasta] Chl1p [Saccharomyces cerevisiae]gi\|683782\|emb\|CAA88378.1\| Chl1p [Saccharomyces cerevisiae]gi\|1314068\|emb\|CAA95033.1\| Chl1p [Saccharomyces cerevisiae]gi\|3538\|emb\|CAA39922.1\| CHL1 [Saccharomyces cerevisiae]gi\|116351\|sp\|P22516\|CHL1_YEAST CHL1 proteingi\| [nrdb_11152004_Chlamydomonas] CHL1 SGDID:S000005929, Chr XVI from 539382-541967, Verified ORF, "Conserved nuclear protein required to establish sister-chromatid pairing during S-phase, probable DNA helicase with similarity to human BACH1, which associates with tumor suppressor BRCA1; [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Conserved nuclear protein required to establish sister-chromatid pairing during S-phase, probable DNA helicase with similarity to human BACH1, which associates with tumor suppressor BRCA1; associates with acetyltransferase Ctf7p; Chl1p [Saccharomyces cere [nr-271106-contam.fasta] Chl1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Probable ATP-dependent RNA helicase CHL1 [nr-271106-contam.fasta] Chl1p [nr-271106-contam.fasta] CHL1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] CHL1 SGDID:S000005929, Chr XVI from 539382-541967, Verified ORF, "Conserved nuclear protein required to establish sister-chromatid pairing during S-phase, probable DNA helicase with similarity to human BACH1, which associates with tumor suppressor BRCA1; associates with acetyltransferase Ctf7p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] ATP-dependent RNA helicase CHL1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CHL1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CHL1 SGDID:S000005929, Chr XVI from 539385-541970, Genome Release 64-1-1, Verified ORF, "Probable DNA helicase involved in sister-chromatid cohesion and genome integrity; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member" [yeast-030211-contam.fasta] CHL1 SGDID:S000005929, Chr XVI from 539385-541970, Genome Release 64-2-1, Verified ORF, "Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome" [20141208_orf_trans.fasta] YPL008W SGDID:S000005929, Chr XVI from 539385-541970, Genome Release 64-1-1, Verified ORF, "Probable DNA helicase involved in sister-chromatid cohesion and genome integrity; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ATP-dependent DNA helicase CHL1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CHL1 PE=1 SV=1 [uniprot_sprot.fasta.20160308]
Organism:	Saccharomyces cerevisiae
Length:	861 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA ]
Biological Process:	mitotic sister chromatid cohesion [IGI][IMP ] chromosome segregation [IDA] donor selection [IGI][IMP ]
Molecular Function:	DNA helicase activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDKKEYSETF YHPYKPYDIQ VQLMETVYRV LSEGKKIAIL ESPTGTGKTL SLICATMTWL  60
   61 RMNKADIFTR METNIKTNED DSENLSDDEP DWVIDTYRKS VLQEKVDLLN DYEKHLNEIN 120
  121 TTSCKQLKTM CDLDKEHGRY KSVDPLRKKR KGARHLDVSL EEQDFIPRPY ESDSENNDTS 180
  181 KSTRGGRISD KDYKLSELNS QIITLLDKID GKVSRDPNNG DRFDVTNQNP VKIYYASRTY 240
  241 SQLGQFTSQL RLPSFPSSFR DKVPDEKVKY LPLASKKQLC INPKVMKWKT LEAINDACAD 300
  301 LRHSKEGCIF YQNTNEWRHC PDTLALRDMI FSEIQDIEDL VPLGKSLGIC PYYASREALP 360
  361 IAEVVTLPYQ YLLSESTRSS LQINLENSIV IIDEAHNLIE TINSIYSSQI SLEDLKNCHK 420
  421 GIVTYFNKFK SRLNPGNRVN LLKLNSLLMT LIQFIVKNFK KIGQEIDPND MFTGSNIDTL 480
  481 NIHKLLRYIK VSKIAYKIDT YNQALKEEES SKNENPIKET HKKSVSSQPL LFKVSQFLYC 540
  541 LTNLTSEGQF FFEKNYSIKY MLLEPSKPFE SILNQAKCVV LAGGTMEPMS EFLSNLLPEV 600
  601 PSEDITTLSC NHVIPKENLQ TYITNQPELE FTFEKRMSPS LVNNHLFQFF VDLSKAVPKK 660
  661 GGIVAFFPSY QYLAHVIQCW KQNDRFATLN NVRKIFYEAK DGDDILSGYS DSVAEGRGSL 720
  721 LLAIVGGKLS EGINFQDDLC RAVVMVGLPF PNIFSGELIV KRKHLAAKIM KSGGTEEEAS 780
  781 RATKEFMENI CMKAVNQSVG RAIRHANDYA NIYLLDVRYN RPNFRKKLSR WVQDSINSEH 840
  841 TTHQVISSTR KFFSMRSLNS R

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SGT1	CHL1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CHL1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..75]	MSA	2.07099	View MSA. Confident ab initio structure predictions are available.
2	View Details	[76..148]	deduced	N/A	No confident structure predictions are available.
3	View Details	[149..263]	PSI-BLAST	3.522879	Putative DEAD box RNA helicase
4	View Details	[264..410]	deduced	N/A	No confident structure predictions are available.
5	View Details	[411..861]	MSA	13.187997	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	5.47805400577115	bayes_pls_golite062009
DNA-dependent ATPase activity	2.72462789867829	bayes_pls_golite062009
deoxyribonuclease activity	2.72222787956584	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
endodeoxyribonuclease activity	2.60873257753475	bayes_pls_golite062009
hydrolase activity	2.55157966585974	bayes_pls_golite062009
nucleic acid binding	2.51683451033161	bayes_pls_golite062009
dATP binding	2.44149059794577	bayes_pls_golite062009
DNA helicase activity	2.2817164193758	bayes_pls_golite062009
pyrophosphatase activity	2.13284212711072	bayes_pls_golite062009
DNA binding	2.12258000781149	bayes_pls_golite062009
transcription regulator activity	2.09345198747025	bayes_pls_golite062009
endonuclease activity	1.96689197136388	bayes_pls_golite062009
ATP-dependent DNA helicase activity	1.88455339138556	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
microtubule motor activity	1.6537049553461	bayes_pls_golite062009
RNA helicase activity	1.63960194106079	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.61735400124552	bayes_pls_golite062009
RNA-dependent ATPase activity	1.60404584546501	bayes_pls_golite062009
double-strand/single-strand DNA junction binding	1.47358115221023	bayes_pls_golite062009
purine deoxyribonucleotide binding	1.4428455823518	bayes_pls_golite062009
adenyl deoxyribonucleotide binding	1.4428455823518	bayes_pls_golite062009
protein-DNA loading ATPase activity	1.29640539057136	bayes_pls_golite062009
structure-specific DNA binding	1.2539059700735	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
motor activity	1.14667913749579	bayes_pls_golite062009
DNA clamp loader activity	1.12431517057397	bayes_pls_golite062009
double-stranded DNA binding	1.117585097627	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
transcription factor activity	0.887108427603557	bayes_pls_golite062009
transcription repressor activity	0.862685397469217	bayes_pls_golite062009
single-stranded DNA binding	0.568457189190858	bayes_pls_golite062009
cytoskeletal protein binding	0.500462538667228	bayes_pls_golite062009
histone acetyltransferase activity	0.328186246219424	bayes_pls_golite062009
lysine N-acetyltransferase activity	0.328186246219424	bayes_pls_golite062009
mismatched DNA binding	0.31144582840038	bayes_pls_golite062009
DNA strand annealing activity	0.283577486331079	bayes_pls_golite062009
four-way junction helicase activity	0.26252931460003	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.243902356228423	bayes_pls_golite062009
exodeoxyribonuclease V activity	0.185603938981231	bayes_pls_golite062009
nucleotidyltransferase activity	0.181509771587324	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.147579541298736	bayes_pls_golite062009
nucleotide binding	0.114848452385726	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.113504049290503	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.111293884322157	bayes_pls_golite062009
purine nucleotide binding	0.10713474095071	bayes_pls_golite062009
purine ribonucleotide binding	0.0916519414118148	bayes_pls_golite062009
ribonucleotide binding	0.0915913646217636	bayes_pls_golite062009
structural constituent of ribosome	0.089234257580272	bayes_pls_golite062009
3'-5' DNA helicase activity	0.0769112340058804	bayes_pls_golite062009
histone methyltransferase activity (H3-K9 specific)	0.0642842984371965	bayes_pls_golite062009
actin binding	0.00482938540552702	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2