Protein Overview: DOA1

General Information:
Name(s) found:	DOA1 / YKL213C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322636\|ref\|NP_012709.1\| WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; Doa1p [NCBI NR] gi\|549752\|sp\|P36037.1\|DOA1_YEAST RecName: Full=Protein DOA1 [NCBI NR] pir\|\|S38051 DOA1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|486381\|emb\|CAA82058.1\| DOA1 [Saccharomyces cerevisiae] [NCBI NR] gi\|473137\|emb\|CAA53560.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1086570\|gb\|AAA82258.1\| Doa1p [NCBI NR] Protein DOA1 OS=Saccharomyces cerevisiae GN=DOA1 PE=1 SV=1 [Swiss-Prot] DOA1 Protein involved in ubiquitin-dependent proteolysis [mips] WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase [SGD] DOA1, Chr XI from 31961-34108, reverse complement [yeast_orfs2] Chr XI from 31961-34108, reverse complement [yeastorf3.fasta] Chr XI from 34108-31961, reverse complement [yeastorf5.fasta] (P36037) DOA1 protein [4932.FASTACCERV.txt] DOA1 SGDID:S0001696, Chr XI from 34108-31961, reverse complement, Verified ORF [yeastflysequest.fasta] Doa1p [Saccharomyces cerevisiae]gi\|473137\|emb\|CAA53560.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|486381\|emb\|CAA82058.1\| DOA1 [Saccharomyces cerevisiae]gi\|549752\|sp\|P36037\|DOA1_YEAST DOA1 proteingi\|539311\|pir\|\|S38051 DOA1 protein - yeast [nrdb_11152004_Chlamydomonas] DOA1 SGDID:S000001696, Chr XI from 34108-31961, reverse complement, Verified ORF, "WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promote [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein DOA1 [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; Doa1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DOA1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Doa1p [nr-271106-contam.fasta] DOA1 SGDID:S000001696, Chr XI from 34108-31961, reverse complement, Verified ORF, "WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Protein DOA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DOA1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DOA1 SGDID:S000001696, Chr XI from 34107-31960, Genome Release 64-1-1, reverse complement, Verified ORF, "WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase" [yeast-030211-contam.fasta] DOA1 SGDID:S000001696, Chr XI from 34107-31960, Genome Release 64-2-1, reverse complement, Verified ORF, "WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; facilitates N-terminus-dependent proteolysis of centromeric histone H3 (Cse4p) for faithful chromosome segregation; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress" [20141208_orf_trans.fasta] YKL213C SGDID:S000001696, Chr XI from 34107-31960, Genome Release 64-1-1, reverse complement, Verified ORF, "WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	715 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	double-strand break repair via nonhomologous end joining [IMP] ubiquitin cycle [IGI][IMP][IPI] ubiquitin-dependent protein catabolic process [IMP]
Molecular Function:	ubiquitin binding [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGYQLSATLK GHDQDVRDVV AVDDSKVASV SRDGTVRLWS KDDQWLGTVV YTGQGFLNSV  60
   61 CYDSEKELLL FGGKDTMING VPLFATSGED PLYTLIGHQG NVCSLSFQDG VVISGSWDKT 120
  121 AKVWKEGSLV YNLQAHNASV WDAKVVSFSE NKFLTASADK TIKLWQNDKV IKTFSGIHND 180
  181 VVRHLAVVDD GHFISCSNDG LIKLVDMHTG DVLRTYEGHE SFVYCIKLLP NGDIVSCGED 240
  241 RTVRIWSKEN GSLKQVITLP AISIWSVDCM SNGDIIVGSS DNLVRIFSQE KSRWASEDEI 300
  301 NELSTQVEKS TISSKTIEFD ESKLSPYEIL QSPGRKEGQI VVVKSPQGTI EAHQFSNSSW 360
  361 KKVGDVVGAG ATGNDKKIEF EGKTYDYVFD VDIEDGKPPL KLPINVSDNP YTAADNFLAR 420
  421 YELPMSYRDQ VVQFILKNTN GISLDQPNDN ASSSAVSPSK TSVMKVLPVK QYLIMENYNP 480
  481 DTIFNGIVKI NSNEKTFDDE ILAQIGGALH DIDESWELLL SFANTIRSNW EIKTPAYDIV 540
  541 RLIVKKLPYS SDIKDYIEEG LGNKNITLTM LTVRILVNCF NNENWGVKLL ESNQVYKSIF 600
  601 ETIDTEFSQA SAKQSQNLAI AVSTLIFNYS ALVTKGNSDL ELLPIVADAI NTKYGPLEEY 660
  661 QECEEAAYRL TVAYGNLATV EPTLRQFANS VTWLANIKRS YGNVPRFKDI FDDLS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CDC48 DOA1
View Details	Krogan NJ, et al. (2006)		BFA1 CDC48 DOA1 FBP1 MRM1 NDT80 NPL4 OTU1 RDS2 SHP1 TEM1 TUP1 UBX4 UBX5 UBX7 UFD1 UFD2 YDR049W YGL108C YJR098C YNL155W YOR342C
View Details	Gavin AC, et al. (2002)		BNI1 CDC48 DOA1 MRPS28 NPL4 PDC1 RAI1 REG1 RPA135 RPA190 RPO31 SHP1 UFD1 YDR049W

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	UBX6	DOA1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	DOA1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..89]	PSI-BLAST	149.626727	Tup1, C-terminal domain
2	View Details	[90..393]	PSI-BLAST	58.190949	Groucho/tle1, C-terminal domain
3	View Details	[394..491]	PSI-BLAST	17.0	Arp2/3 complex 41 kDa subunit ARPC1
4	View Details	[492..715]	deduced	N/A	No confident structure predictions are available.
5	View Details	[515..715]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2