Protein Overview: UBA4

General Information:
Name(s) found:	UBA4 / YHR111W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|731699\|sp\|P38820.1\|UBA4_YEAST RecName: Full=Adenylyltransferase and sulfurtransferase UBA4; AltName: Full=Ubiquitin-like protein activator 4; AltName: Full=Needs CLA4 to survive protein 3; Includes: RecName: Full=Adenylyltransferase UBA4; Includes: Rec [NCBI NR] gi\|6321903\|ref\|NP_011979.1\| Protein that activates Urm1p before its conjugation to proteins (urmylation); one target is the thioredoxin peroxidase Ahp1p, suggesting a role of urmylation in the oxidative stress response; Uba4p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S48953 hypothetical protein YHR111w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|529119\|gb\|AAB68852.1\| Yhr111wp [Saccharomyces cerevisiae] [NCBI NR] (P38820) Hypothetical 49.4 kDa protein in CDC12-ORC6 intergenic region [Swiss-Prot] Adenylyltransferase and sulfurtransferase UBA4 OS=Saccharomyces cerevisiae GN=UBA4 PE=1 SV=1 [Swiss-Prot] UBA4 Protein that activates Urm1p before its conjugation to proteins [mips] Protein that activates Urm1p before its conjugation to proteins (urmylation); one target is the thioredoxin peroxidase Ahp1p, suggesting a role of urmylation in the oxidative stress response [SGD] YHR111W, Chr VIII from 333074-334396 [yeast_orfs2] Chr VIII from 333074-334396 [yeastorf3.fasta] UBA4 SGDID:S0001153, Chr VIII from 333074-334396, Verified ORF [yeastflysequest.fasta] Protein that activates Urm1p before its conjugation to proteins (urmylation); one target is the thioredoxin peroxidase Ahp1p, suggesting a role of urmylation in the oxidative stress response [Saccharomyces cerevisiae]gi\|731699\|sp\|P38820\|YHR1_YEAST Hypoth [nrdb_11152004_Chlamydomonas] UBA4 SGDID:S000001153, Chr VIII from 333075-334397, Verified ORF, "Protein that activates Urm1p before its conjugation to proteins (urmylation); one target is the thioredoxin peroxidase Ahp1p, suggesting a role of urmylation in the oxidative stress respon [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein that activates Urm1p before its conjugation to proteins (urmylation); one target is the thioredoxin peroxidase Ahp1p, suggesting a role of urmylation in the oxidative stress response; Uba4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Yhr111wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] E1-like URM1 activator protein (Ubiquitin-like protein activator 4) [nr-271106-contam.fasta] UBA4 SGDID:S000001153, Chr VIII from 333075-334397, Verified ORF, "Protein that activates Urm1p before its conjugation to proteins (urmylation); one target is the thioredoxin peroxidase Ahp1p, suggesting a role of urmylation in the oxidative stress response" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Adenylyltransferase and sulfurtransferase UBA4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=UBA4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] UBA4 SGDID:S000001153, Chr VIII from 333072-334394, Genome Release 64-1-1, Verified ORF, "Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p" [yeast-030211-contam.fasta] UBA4 SGDID:S000001153, Chr VIII from 333072-334394, Genome Release 64-2-1, Verified ORF, "E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p" [20141208_orf_trans.fasta] TR287\|c0_g1_i1\|g.200 ORF TR287\|c0_g1_i1\|g.200 TR287\|c0_g1_i1\|m.200 type:complete len:441 (+) TR287\|c0_g1_i1:818-2140(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YHR111W SGDID:S000001153, Chr VIII from 333072-334394, Genome Release 64-1-1, Verified ORF, "Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_8230\|g.5041 ORF ID=asmbl_8230\|g.5041 ID=asmbl_8230\|m.5041 type:complete len:441 (+) ID=asmbl_8230:2788-4110(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	440 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	response to oxidative stress [IMP] protein modification process [IDA][IMP] pseudohyphal growth [IMP] cell budding [IGI] invasive growth in response to glucose limitation [IGI][IMP]
Molecular Function:	URM1 activating enzyme activity [IDA][IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNDYHLEDTT SELEALRLEN AQLREQLAKR EDSSRDYPLS LEEYQRYGRQ MIVEETGGVA  60
   61 GQVKLKNTKV LVVGAGGLGC PALPYLAGAG VGQIGIVDND VVETSNLHRQ VLHDSSRVGM 120
  121 LKCESARQYI TKLNPHINVV TYPVRLNSSN AFDIFKGYNY ILDCTDSPLT RYLVSDVAVN 180
  181 LGITVVSASG LGTEGQLTIL NFNNIGPCYR CFYPTPPPPN AVTSCQEGGV IGPCIGLVGT 240
  241 MMAVETLKLI LGIYTNENFS PFLMLYSGFP QQSLRTFKMR GRQEKCLCCG KNRTITKEAI 300
  301 EKGEINYELF CGARNYNVCE PDERISVDAF QRIYKDDEFL AKHIFLDVRP SHHYEISHFP 360
  361 EAVNIPIKNL RDMNGDLKKL QEKLPSVEKD SNIVILCRYG NDSQLATRLL KDKFGFSNVR 420
  421 DVRGGYFKYI DDIDQTIPKY

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		GUS1 QRI1 SLX4 UBA4 YOR097C

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SNX41	UBA4	2	Unpublished Fields Lab Data
View Screen	MDM1	UBA4	1	Unpublished Fields Lab Data
View Screen	PFK2	UBA4	1	Unpublished Fields Lab Data
View Screen	APC5	UBA4	1	Unpublished Fields Lab Data
View Screen	RAD1	UBA4	1	Unpublished Fields Lab Data
View Screen	FUN19	UBA4	1	Unpublished Fields Lab Data
View Screen	GPA1	UBA4	1	Unpublished Fields Lab Data
View Screen	IRE1	UBA4	1	Unpublished Fields Lab Data
View Screen	GPI16	UBA4	1	Unpublished Fields Lab Data
View Screen	MET30	UBA4	1	Unpublished Fields Lab Data
View Screen	TPK3	UBA4	1	Unpublished Fields Lab Data
View Screen	STE3	UBA4	1	Unpublished Fields Lab Data
View Screen	TEM1	UBA4	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	UBA4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..290]	PSI-BLAST	443.9691	Molybdenum cofactor biosynthesis protein MoeB
2	View Details	[291..440]	PSI-BLAST	17.154902	Sulfurtransferase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
URM1 activating enzyme activity	5.44388923879076	bayes_pls_golite062009
small protein activating enzyme activity	3.58068602287881	bayes_pls_golite062009
NEDD8 activating enzyme activity	2.11391077579348	bayes_pls_golite062009
thiosulfate sulfurtransferase activity	2.05168889065215	bayes_pls_golite062009
small conjugating protein ligase activity	1.84216910649062	bayes_pls_golite062009
binding	1.46180162036652	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.31244773198273	bayes_pls_golite062009
acid-amino acid ligase activity	1.27535252106053	bayes_pls_golite062009
transcription regulator activity	1.23669539775752	bayes_pls_golite062009
catalytic activity	1.22201444897049	bayes_pls_golite062009
ligase activity	0.886082936938522	bayes_pls_golite062009
transferase activity	0.680338489823338	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.302798969355962	bayes_pls_golite062009
protein binding	0.258795594127926	bayes_pls_golite062009

Term	Confidence	Notes
thiosulfate sulfurtransferase activity	4.07143118207301	bayes_pls_golite062009
binding	1.53109235820839	bayes_pls_golite062009
transferase activity	1.00600613068372	bayes_pls_golite062009
phosphoprotein phosphatase activity	0.765055251775806	bayes_pls_golite062009
catalytic activity	0.687308230067573	bayes_pls_golite062009
phosphatase activity	0.596156043915887	bayes_pls_golite062009
sulfurtransferase activity	0.419061082217665	bayes_pls_golite062009
protein binding	0.415782692658192	bayes_pls_golite062009
phosphoric ester hydrolase activity	0.41208200336081	bayes_pls_golite062009
protein tyrosine phosphatase activity	0.224132693583958	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.83

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2