Protein Overview: RAD2

General Information:
Name(s) found:	RAD2 / YGR258C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|A29839 RAD2 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6321697\|ref\|NP_011774.1\| Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein; Rad2p [Saccharomyces ce [NCBI NR] gi\|172329\|gb\|AAA66928.1\| RAD2 [Saccharomyces cerevisiae] [NCBI NR] gi\|1323470\|emb\|CAA97287.1\| RAD2 [Saccharomyces cerevisiae] [NCBI NR] gi\|131811\|sp\|P07276.2\|RAD2_YEAST RecName: Full=DNA repair protein RAD2 [NCBI NR] DNA repair protein RAD2 OS=Saccharomyces cerevisiae GN=RAD2 PE=1 SV=2 [Swiss-Prot] RAD2 Structure-specific nuclease of the nucleotide excision repairosome [mips] Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein [SGD] RAD2, Chr VII from 1007668-1010763, reverse complement [yeast_orfs2] Chr VII from 1007668-1010763, reverse complement [yeastorf3.fasta] Chr VII from 1007675-1010770, reverse complement [yeastorf4.fasta] Chr VII from 1010770-1007675, reverse complement [yeastorf5.fasta] (P07276) DNA repair protein RAD2 [4932.FASTACCERV.txt] RAD2 SGDID:S0003490, Chr VII from 1010771-1007676, reverse complement, Verified ORF [yeastflysequest.fasta] RAD2 SGDID:S0003490, Chr VII from 1010772-1007677, reverse complement, Verified ORF [10112005cervSGD.fasta] Rad2p [Saccharomyces cerevisiae]gi\|1323470\|emb\|CAA97287.1\| RAD2 [Saccharomyces cerevisiae]gi\|83443\|pir\|\|A29839 RAD2 protein - yeast (Saccharomyces cerevisiae)gi\|131811\|sp\|P07276\|RAD2_YEAST DNA repair protein RAD2gi\|172329\|gb\|AAA66928.1\| RAD2 [nrdb_11152004_Chlamydomonas] RAD2 SGDID:S000003490, Chr VII from 1010772-1007677, reverse complement, Verified ORF, "Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 ( [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RAD2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA-repair protein RAD2 [nr-271106-contam.fasta] Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein; Rad2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RAD2 [nr-271106-contam.fasta] RAD2 SGDID:S000003490, Chr VII from 1010772-1007677, reverse complement, Verified ORF, "Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein" [yeast-bovrabprots.fasta] [chemostatdb.fasta] DNA repair protein RAD2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RAD2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RAD2 SGDID:S000003490, Chr VII from 1010766-1007671, Genome Release 64-1-1, reverse complement, Verified ORF, "Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein" [yeast-030211-contam.fasta] RAD2 SGDID:S000003490, Chr VII from 1010766-1007671, Genome Release 64-2-1, reverse complement, Verified ORF, "Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein" [20141208_orf_trans.fasta] YGR258C SGDID:S000003490, Chr VII from 1010766-1007671, Genome Release 64-1-1, reverse complement, Verified ORF, "Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1031 amino acids
Gene Ontology:
Cellular Component:	nucleotide-excision repair factor 3 complex [IDA]
Biological Process:	nucleotide-excision repair, DNA incision, 3'-to lesion [TAS] nucleotide-excision repair [IDA]
Molecular Function:	single-stranded DNA specific endodeoxyribonuclease activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGVHSFWDIA GPTARPVRLE SLEDKRMAVD ASIWIYQFLK AVRDQEGNAV KNSHITGFFR  60
   61 RICKLLYFGI RPVFVFDGGV PVLKRETIRQ RKERRQGKRE SAKSTARKLL ALQLQNGSND 120
  121 NVKNSTPSSG SSVQIFKPQD EWDLPDIPGF KYDKEDARVN SNKTFEKLMN SINGDGLEDI 180
  181 DLDTINPASA EFEELPKATQ YLILSSLRLK SRLRMGYSKE QLETIFPNSM DFSRFQIDMV 240
  241 KRRNFFTQKL INTTGFQDGG ASKLNEEVIN RISGQKSKEY KLTKTNNGWI LGLGANDGSD 300
  301 AQKAIVIDDK DAGALVKQLD SNAEDGDVLR WDDLEDNSLK IVRHESSNAT TAPQKRSNRS 360
  361 EDEGCDSDEC EWEEVELKPK NVKFVEDFSL KAARLPYMGQ SLNNAGSKSF LDKRHDQASP 420
  421 SKTTPTMRIS RISVEDDDED YLKQIEEIEM MEAVQLSKME KKPEADDKSK IAKPVTSKGT 480
  481 EARPPIVQYG LLGAQPDSKQ PYHVTNLNSK SESVIKRTSK TVLSEFRPPS QQEDKGAILT 540
  541 EGEQNLNFIS HKIPQFDFNN ENSLLFQKNT ESNVSQEATK EKSPIPEMPS WFSSTASQQL 600
  601 YNPYNTTNFV EDKNVRNEQE SGAETTNKGS SYELLTGLNA TEILERESEK ESSNDENKDD 660
  661 DLEVLSEELF EDVPTKSQIS KEAEDNDSRK VESINKEHRK PLIFDYDFSE DEEDNIVENM 720
  721 IKEQEEFDTF KNTTLSTSAE RNVAENAFVE DELFEQQMKD KRDSDEVTMD MIKEVQELLS 780
  781 RFGIPYITAP MEAEAQCAEL LQLNLVDGII TDDSDVFLFG GTKIYKNMFH EKNYVEFYDA 840
  841 ESILKLLGLD RKNMIELAQL LGSDYTNGLK GMGPVSSIEV IAEFGNLKNF KDWYNNGQFD 900
  901 KRKQETENKF EKDLRKKLVN NEIILDDDFP SVMVYDAYMR PEVDHDTTPF VWGVPDLDML 960
  961 RSFMKTQLGW PHEKSDEILI PLIRDVNKRK KKGKQKRINE FFPREYISGD KKLNTSKRIS1020
 1021 TATGKLKKRK M

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		CCL1 KIN28 RAD2 RAD3 SSL1 SSL2 TFB1 TFB2 TFB3
View Details	Ho Y, et al. (2002)		PEX15 RAD2
View Details	Qiu et al. (2008)		CCL1 RAD1 RAD16 RAD2 RAD3 RAD7 SSL1 SSL2 TFB3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	RAD2	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
single-stranded DNA specific endodeoxyribonuclease activity	8.219187336366	bayes_pls_golite062009
endodeoxyribonuclease activity	7.26787266873051	bayes_pls_golite062009
deoxyribonuclease activity	6.75316483671775	bayes_pls_golite062009
endonuclease activity	6.39053146471031	bayes_pls_golite062009
exonuclease activity	3.46607201774246	bayes_pls_golite062009
flap endonuclease activity	3.35117165021395	bayes_pls_golite062009
DNA binding	2.74395891269151	bayes_pls_golite062009
hydrolase activity	2.60874360745066	bayes_pls_golite062009
binding	2.58539672241253	bayes_pls_golite062009
nucleic acid binding	2.54722210455433	bayes_pls_golite062009
5'-3' exonuclease activity	2.50129811584994	bayes_pls_golite062009
nuclease activity	2.49612885073648	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	2.20867470281829	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	2.11221521670814	bayes_pls_golite062009
exodeoxyribonuclease activity, producing 5'-phosphomonoesters	2.01491211227289	bayes_pls_golite062009
exodeoxyribonuclease activity	2.00804590500192	bayes_pls_golite062009
damaged DNA binding	1.82833131243547	bayes_pls_golite062009
5'-3' exodeoxyribonuclease activity	1.59902329953921	bayes_pls_golite062009
structure-specific DNA binding	1.57088273902548	bayes_pls_golite062009
double-stranded DNA specific exodeoxyribonuclease activity	1.46817649825808	bayes_pls_golite062009
catalytic activity	1.27951740231695	bayes_pls_golite062009
5'-flap endonuclease activity	1.13738790511505	bayes_pls_golite062009
single-stranded DNA specific exodeoxyribonuclease activity	1.06918533266108	bayes_pls_golite062009
double-stranded DNA binding	0.550147498650064	bayes_pls_golite062009
single-stranded DNA specific 5'-3' exodeoxyribonuclease activity	0.496731274592508	bayes_pls_golite062009
endodeoxyribonuclease activity, producing 5'-phosphomonoesters	0.330551387282259	bayes_pls_golite062009
protein binding	0.235717056815183	bayes_pls_golite062009
3'-5' exonuclease activity	0.175191448555725	bayes_pls_golite062009
endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.0756835782024594	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.00167240601151697	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
single-stranded DNA specific endodeoxyribonuclease activity	8.51020676030278	bayes_pls_golite062009
endodeoxyribonuclease activity	7.84678654948641	bayes_pls_golite062009
deoxyribonuclease activity	7.26876236760767	bayes_pls_golite062009
endonuclease activity	6.8680936339396	bayes_pls_golite062009
flap endonuclease activity	4.10438637013244	bayes_pls_golite062009
exonuclease activity	3.99717729574528	bayes_pls_golite062009
5'-3' exonuclease activity	3.19536858640519	bayes_pls_golite062009
nuclease activity	2.9643999717473	bayes_pls_golite062009
DNA binding	2.84879111682833	bayes_pls_golite062009
hydrolase activity	2.80884894948531	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	2.66071760609476	bayes_pls_golite062009
nucleic acid binding	2.62833003756567	bayes_pls_golite062009
binding	2.59149897255998	bayes_pls_golite062009
exodeoxyribonuclease activity, producing 5'-phosphomonoesters	2.50398903419616	bayes_pls_golite062009
exodeoxyribonuclease activity	2.49591627688404	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	2.45364009363271	bayes_pls_golite062009
5'-3' exodeoxyribonuclease activity	2.26658239783943	bayes_pls_golite062009
damaged DNA binding	2.10834170214137	bayes_pls_golite062009
double-stranded DNA specific exodeoxyribonuclease activity	2.09057967200253	bayes_pls_golite062009
5'-flap endonuclease activity	1.81850767854435	bayes_pls_golite062009
structure-specific DNA binding	1.70042864382698	bayes_pls_golite062009
single-stranded DNA specific exodeoxyribonuclease activity	1.61167902830399	bayes_pls_golite062009
catalytic activity	1.37534898662234	bayes_pls_golite062009
single-stranded DNA specific 5'-3' exodeoxyribonuclease activity	1.15948049342759	bayes_pls_golite062009
endodeoxyribonuclease activity, producing 5'-phosphomonoesters	0.901965019685213	bayes_pls_golite062009
double-stranded DNA binding	0.626785110524684	bayes_pls_golite062009
endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.512509234493511	bayes_pls_golite062009
3'-5' exonuclease activity	0.50487165366129	bayes_pls_golite062009
protein binding	0.236485289446406	bayes_pls_golite062009
single-stranded DNA specific 3'-5' exodeoxyribonuclease activity	0.14861832363159	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.0941467407417242	bayes_pls_golite062009
DNA polymerase activity	0.0691107435153178	bayes_pls_golite062009
3'-5'-exodeoxyribonuclease activity	0.0205129272358	bayes_pls_golite062009

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..195]	ORFEUS	12.55	5' to 3' exonuclease domain of DNA polymerase Taq; Exonuclease domain of prokaryotic DNA polymerase; DNA polymerase I (Klenow fragment)
2	View Details	[196..647]	MSA	32.249997	View MSA. No confident structure predictions are available.
3	View Details	[648..1031]	PSI-BLAST	166.9897	Flap endonuclease-1 (Fen-1 nuclease)
4	View Details	[671..1031]	PSI-BLAST	72.522879	Crystal structure of the human FEN1-PCNA complex

Protein Overview: RAD2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: