Protein Overview: MTC5

General Information:
Name(s) found:	MTC5 / YDR128W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74676351\|sp\|Q03897.1\|YD128_YEAST RecName: Full=WD repeat-containing protein YDR128W [NCBI NR] gi\|665660\|emb\|CAA88209.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6320333\|ref\|NP_010413.1\| Putative protein of unknown function; Ydr128wp [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S51855 hypothetical protein YDR128w - yeast (Saccharomyces cerevisiae) [NCBI NR] WD repeat-containing protein YDR128W OS=Saccharomyces cerevisiae GN=YDR128W PE=1 SV=1 [Swiss-Prot] Protein of unknown function localised to vacuolar membrane [mips] Protein of unknown function; mtc5 is synthetically sick with cdc13-1 [SGD] YDR128W, Chr IV from 709542-712988 [yeast_orfs2] Chr IV from 709542-712988 [yeastorf3.fasta] >Q03897 (Q03897) Hypothetical protein [4932.FASTACCERV.txt] YDR128W SGDID:S0002535, Chr IV from 709543-712989, Uncharacterized ORF [yeastflysequest.fasta] YDR128W SGDID:S0002535, Chr IV from 709544-712990, Uncharacterized ORF [10112005cervSGD.fasta] Ydr128wp [Saccharomyces cerevisiae]gi\|665660\|emb\|CAA88209.1\| unknown [Saccharomyces cerevisiae]gi\|1077527\|pir\|\|S51855 hypothetical protein YDR128w - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] (Q03897) Hypothetical protein [cerv4932.FASTAC] YDR128W SGDID:S000002535, Chr IV from 709544-712990, Uncharacterized ORF, "Hypothetical protein" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YDR128W SGDID:S000002535, Chr IV from 709546-712992, Uncharacterized ORF, "Hypothetical protein" [yeast-contam.fasta] YDR128W SGDID:S000002535, Chr IV from 709547-712993, Uncharacterized ORF, "Putative protein of unknown function; YDR128W is synthetically sick with cdc13-1" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] MTC5 SGDID:S000002535, Chr IV from 709547-712993, Verified ORF, "Protein of unknown function; mtc5 is synthetically sick with cdc13-1" [yeast-200209-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] hypothetical protein; Ydr128wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] WD repeat protein YDR128W [nr-271106-contam.fasta] [chemostatdb.fasta] MTC5 SGDID:S000002535, Chr IV from 709549-712995, Genome Release 64-1-1, Verified ORF, "Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1" [yeast-030211-contam.fasta] MTC5 SGDID:S000002535, Chr IV from 709549-712995, Genome Release 64-2-1, Verified ORF, "Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress" [20141208_orf_trans.fasta] YDR128W SGDID:S000002535, Chr IV from 709549-712995, Genome Release 64-1-1, Verified ORF, "Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1148 amino acids
Gene Ontology:
Cellular Component:	fungal-type vacuole membrane [IDA]
Biological Process:	biological_process [ND]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MCSSINEGPY NSPTFGKSLS LKVDGGFNAV SINPSGRDIV LASRQGLYII DLDDPFTPPR  60
   61 WLHHITPWQV ADVQWSPHPA KPYWIVSTSN QKAIIWNLAK SSSNAIEFVL HGHSRAITDI 120
  121 NFNPQHPDVL ATCSVDTYVH AWDMRSPHRP FYSTSSWRSA ASQVKWNYKD PNVLASSHGN 180
  181 DIFVWDLRKG STPLCSLKGH VSSVNSIDFN RFKYSEIMSS SNDGTVKFWD YSKSTTESKR 240
  241 TVTTNFPIWR GRYLPFGEGY CIMPMVGGNN AVYLINLCDD DDSEQNKKTK LQPIYAFKGH 300
  301 SDRVIDFLWR SRHTCDGDYD DREFQLVTWS KDCDLKLWPI SDSIYGKVNF DRGKRLEEKL 360
  361 PDYDYCSYNK EPENRENVQK NEFRRLRENF VTTSGLKKNK TNHITWLSGI RMNSATSQED 420
  421 LFNETKIQNL GEEVSAIGHK FPKVVFEKIS VSTRELCLTL NGPWSEENPD DYIFLRISIN 480
  481 FPLNYPNKGD PPKFTIEENS NLTMSKRQEI LSNLATIGQK YTDSNLYCLE PCIRFVLGEK 540
  541 VSLEDIEEGQ EPLLNFDIAD HIDFEELSSL DSSYSDSQNP ENLSSQSDIE SYKEALVFPD 600
  601 TSNQGLDFGR NLALDTTPVP NGCGSCWTAT GELFCFFANE KKPEKKQNAI IKLSQKEAGV 660
  661 EKHPFKIEPQ VLYDKEVDSS VITAADELKA RPKRYVDTLG LGGGTNGDSR TYFDDETSSD 720
  721 DSFDSVADDW DDILRNDIIV RTKIPILRGN FKAFSSVHSE SGKTVESTKK NKNLVISKNF 780
  781 SSLLSDRKEL ALEYLFMDAT PEGFARNNAL VAEKFDLDEI SHCWQILSDM LIDQSDYDPY 840
  841 TTIWNNHPMG IKWFIKEAIV YFERQQNLQM LAMLCCVILS ARRKKIPARY YGQELENMEG 900
  901 TIVFNDNESQ NTSFWKGSDA FSTRSRSSTV TPNFYGNHLR GKNIHGGDNS SIRSDDHHAR 960
  961 LRTHNTLNGS SKFTEPAQKQ GSRAISSSPF HSRMPDIKVE LLHDDIIEAY EQEDLLHLEV1020
 1021 SDIPKFQTYI YQYSKLLFRW GLPLERVKIL KVSTDFRSSY SSQGIPPNNN KKSPYNGVLT1080
 1081 HWIENNEFGE EKFLARNCNY CDLRVTRSSF ICGNCQHVLH SSCARIWWEI GDECPSGCGC1140
 1141 NCPEMFDA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		CMR2 MTC5
View Details	Ho Y, et al. (2002)		AAC3 APT1 ARO1 CCT6 CDC33 CPR6 CYS4 DSS1 ECM10 FMP10 HEF3 HOR2 HXT7 MDJ1 MTC5 NPR2 OYE2 PGM2 POX1 PRE1 PST2 PUP3 RFA3 RHR2 RNQ1 SEH1 SOD2 THI21 TMA29 TPS1 TSA2 YHR033W YJR141W YKU80

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CWC2	No Comments	Hazbun TR, et al. (2003)
View Run	CAB3	No Comments	Hazbun TR, et al. (2003)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HMT1	MTC5	1	Unpublished Fields Lab Data
View Screen	TUB2	MTC5	1	Unpublished Fields Lab Data
View Screen	MET18	MTC5	1	Unpublished Fields Lab Data
View Screen	UBC7	MTC5	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MTC5	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..346]	PSI-BLAST	74.0	beta1-subunit of the signal-transducing G protein heterotrimer
2	View Details	[347..485]	MSA	2.701996	View MSA. No confident structure predictions are available.
3	View Details	[486..562]	deduced	N/A	No confident structure predictions are available.
4	View Details	[563..832]	MSA	1.032964	View MSA. No confident structure predictions are available.
5	View Details	[833..1063]	deduced	N/A	No confident structure predictions are available.
6	View Details	[1064..1148]	MSA	1.010996	View MSA. No confident structure predictions are available.
7	View Details	[968..1062]	deduced	N/A	No confident structure predictions are available.
8	View Details	[1063..1148]	FFAS03	2.05	No description for 2d8tA was found.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2