Protein Overview: MAP2

General Information:
Name(s) found:	MAP2 / YBL091C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319380\|ref\|NP_009462.1\| Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p; Map2p [Saccharomyces cerevisiae] [NCBI NR] gi\|51704275\|sp\|P38174.3\|AMPM2_YEAST RecName: Full=Methionine aminopeptidase 2; Short=MetAP 2; AltName: Full=Peptidase M 2 [NCBI NR] gi\|1870087\|emb\|CAA84913.1\| MAP2 [Saccharomyces cerevisiae] [NCBI NR] Methionine aminopeptidase 2 OS=Saccharomyces cerevisiae GN=MAP2 PE=1 SV=3 [Swiss-Prot] (P38174) Methionine aminopeptidase 2 (EC 3.4.11.18) (MetAP 2) (Peptidase M 2) [Swiss-Prot] MAP2 Methionine aminopeptidase, isoform 2 [mips] Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p [SGD] MAP2, Chr II from 47357-48622, reverse complement [yeast_orfs2] Chr II from 47357-48622, reverse complement [yeastorf3.fasta] Chr II from 48622-47357, reverse complement [yeastorf5.fasta] MAP2 SGDID:S0000187, Chr II from 48625-47360, reverse complement, Verified ORF [yeastflysequest.fasta] Map2p [Saccharomyces cerevisiae]gi\|1870087\|emb\|CAA84913.1\| MAP2 [Saccharomyces cerevisiae]gi\|51704275\|sp\|P38174\|AMP2_YEAST Methionine aminopeptidase 2 (MetAP 2) (Peptidase M 2) [nrdb_11152004_Chlamydomonas] MAP2 SGDID:S000000187, Chr II from 48625-47360, reverse complement, Verified ORF, "Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MAP2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p; Map2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Methionine aminopeptidase 2 (MetAP 2) (Peptidase M 2) [nr-271106-contam.fasta] [chemostatdb.fasta]
Organism:	Saccharomyces cerevisiae
Length:	421 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [TAS]
Biological Process:	proteolysis [IDA]
Molecular Function:	methionyl aminopeptidase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTDAEIENSP ASDLKELNLE NEGVEQQDQA KADESDPVES KKKKNKKKKK KKSNVKKIEL  60
   61 LFPDGKYPEG AWMDYHQDFN LQRTTVEESR YLKRDLERAE HWNDVRKGAE IHRRVRRAIK 120
  121 DRIVPGMKLM DIADMIENTT RKYTGAENLL AMEDPKSQGI GFPTGLSLNH CAAHFTPNAG 180
  181 DKTVLKYEDV MKVDYGVQVN GNIIDSAFTV SFDPQYDNLL AAVKDATYTG IKEAGIDVRL 240
  241 TDIGEAIQEV MESYEVEING ETYQVKPCRN LCGHSIAPYR IHGGKSVPIV KNGDTTKMEE 300
  301 GEHFAIETFG STGRGYVTAG GEVSHYARSA EDHQVMPTLD SAKNLLKTID RNFGTLPFCR 360
  361 RYLDRLGQEK YLFALNNLVR HGLVQDYPPL NDIPGSYTAQ FEHTILLHAH KKEVVSKGDD 420
  421 Y

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		HKR1 MAP2
View Details	Ho Y, et al. (2002)		MAP2 RFC3 RFC5 RNQ1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PDS1	MAP2	1	Unpublished Fields Lab Data
View Screen	RPN9	MAP2	1	Unpublished Fields Lab Data
View Screen	YHR022C	MAP2	1	Unpublished Fields Lab Data
View Screen	CNB1	MAP2	1	Unpublished Fields Lab Data
View Screen	RRB1	MAP2	1	Unpublished Fields Lab Data
View Screen	SPT21	MAP2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MAP2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..421]	PSI-BLAST	1274.0	Methionine aminopeptidase, insert domain; Methionine aminopeptidase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
peptidase activity	2.60615909541559	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	2.18503136472662	bayes_pls_golite062009
hydrolase activity	1.43646476372001	bayes_pls_golite062009
binding	1.30837875079167	bayes_pls_golite062009
aminopeptidase activity	0.89399203910285	bayes_pls_golite062009
exopeptidase activity	0.762255574851485	bayes_pls_golite062009
metallopeptidase activity	0.629480947971878	bayes_pls_golite062009
nucleic acid binding	0.478507702690501	bayes_pls_golite062009
protein binding	0.378185804009078	bayes_pls_golite062009
transcription regulator activity	0.151893946259744	bayes_pls_golite062009
catalytic activity	0.148551873544128	bayes_pls_golite062009
DNA binding	0.146627179138465	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2