Bait Protein: | DAD1 |
Organism: | Saccharomyces cerevisiae |
Publication: | Shimogawa M. M. et al. (2006) Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase. Curr Biol. 2006 Aug 8;16(15):1489-501. |
Comments: | Dam1 complex purified from yeast with Dad1-TAP |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
SPC19 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 10 | 96 | 67.3% | 0.178 | 18910 |
[ |
DAD1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 7 | 17 | 62.8% | 0.0552 | 10516 |
[ |
ASK1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 19 | 42 | 63.0% | 0.0439 | 32056 |
[ |
SPC34 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 16 | 41 | 51.2% | 0.0425 | 34078 |
[ |
DAD4 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 1 | 10 | 25.0% | 0.0424 | 8155 |
[ |
DAD3 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 7 | 13 | 59.6% | 0.0422 | 10848 |
[ |
TDH3 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... | 26 | 41 | 59.9% | 0.0377 | 35747 |
[ |
DAM1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 19 | 36 | 44.6% | 0.0321 | 38422 |
[ |
DUO1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 13 | 25 | 58.7% | 0.0309 | 27473 |
[ |
TDH2 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... | 19 | 32 | 50.6% | 0.0294 | 35847 |
[ |
RPL23B, RPL23A | Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E... | 6 | 13 | 28.5% | 0.029 | 14473 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 3 | 33 | 9.9% | 0.0278 | 42271 |
[ |
RPS0B | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for mat... | 11 | 15 | 51.6% | 0.0182 | 27962 |
[ |
HSK3 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 3 | 4 | 30.4% | 0.0177 | 8088 |
[ |
RPS0A | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for mat... | 10 | 14 | 42.5% | 0.017 | 28024 |
[ |
RPS7B | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Ap; interacts with K... | 8 | 10 | 34.7% | 0.0161 | 21634 |
[ |
RPL31A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similari... | 3 | 5 | 30.1% | 0.0135 | 12953 |
[ |
RPL31B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similari... | 3 | 5 | 30.1% | 0.0135 | 12967 |
[ |
RPS7A | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with K... | 6 | 7 | 20.0% | 0.0113 | 21622 |
[ |
RPS14A | Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processin... | 4 | 5 | 32.1% | 0.0111 | 14537 |
[ |
RPS14B | Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processin... | 4 | 5 | 31.9% | 0.0111 | 14650 |
[ |
RPS19B | Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... | 3 | 5 | 18.8% | 0.0106 | 15891 |
[ |
RPS19A | Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... | 3 | 5 | 18.8% | 0.0106 | 15917 |
[ |
SSB1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 17 | 21 | 41.4% | 0.0105 | 66602 |
[ |
IPI:IPI00178254.... | 5 | 7 | 40.3% | 0.0101 | 22973 | |
[ |
IPI:IPI00178276.... | 5 | 7 | 40.3% | 0.0101 | 23029 | |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 16 | 21 | 32.1% | 0.01 | 69470 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 16 | 20 | 39.3% | 0.00997 | 66595 |
[ |
IPI:IPI00178198.... | 5 | 7 | 39.5% | 0.00995 | 23530 | |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 14 | 16 | 28.6% | 0.00977 | 54545 |
[ |
RPS5 | Protein component of the small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal ... | 4 | 7 | 15.6% | 0.0095 | 25039 |
[ |
IPI:IPI00178229.... | 5 | 7 | 37.4% | 0.00942 | 24762 | |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 14 | 19 | 27.9% | 0.00904 | 69768 |
[ |
RPL14A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 3 | 4 | 21.7% | 0.00885 | 15167 |
[ |
RPL14B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similari... | 3 | 4 | 21.7% | 0.00885 | 15153 |
[ |
RPL30 | Protein component of the large (60S) ribosomal subunit, has similarity to rat L30 ribosomal protein;... | 2 | 3 | 27.6% | 0.00873 | 11415 |
[ |
CAPNS1 | calpain, small subunit 1 | 4 | 6 | 23.1% | 0.00684 | 28316 |
[ |
IGHG2 | immunoglobulin heavy constant gamma 2 (G2m marker) | 7 | 7 | 27.9% | 0.00656 | 35885 |
[ |
VMA4 | Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 5 | 5 | 23.2% | 0.00655 | 26471 |
[ |
gi|109502777 | gi|109502777|ref|XP_001073968.1| PREDICTED: similar to calmodulin 1 [Rattus norvegicus] | 3 | 3 | 36.9% | 0.00615 | 16815 |
[ |
RPS1B | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarit... | 4 | 5 | 18.4% | 0.00599 | 28812 |
[ |
PSA1 | GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... | 6 | 7 | 24.1% | 0.00592 | 39566 |
[ |
ATP3 | Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily ... | 5 | 6 | 19.9% | 0.00589 | 34351 |
[ |
RPS6A, RPS6B | Protein component of the small (40S) ribosomal subunit; identical to Rps6Bp and has similarity to ra... | 3 | 4 | 13.6% | 0.00518 | 26996 |
[ |
RPL15A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similari... | 2 | 3 | 12.7% | 0.00449 | 24422 |
[ |
RPL15B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Ap and has similari... | 2 | 3 | 12.7% | 0.00449 | 24422 |
[ |
CYS4 | Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... | 7 | 7 | 24.3% | 0.00422 | 56022 |
[ |
SSA3 | ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotrans... | 5 | 8 | 8.6% | 0.00377 | 70547 |
[ |
RPL12B, RPL12A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... | 2 | 2 | 20.6% | 0.0037 | 17823 |
[ |
gi|119581084, gi... | gi|40354192|ref|NP_000412.2| keratin 10 [Homo sapiens], gi|21961605|gb|AAH34697.1| Keratin 10 [Homo ... | 6 | 7 | 19.0% | 0.00366 | 58827 |
[ |
ADH1 | Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... | 4 | 4 | 17.2% | 0.00351 | 36849 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 8 | 10 | 15.8% | 0.00333 | 99619 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 3 | 5 | 14.8% | 0.00333 | 50033 |
[ |
MYLK2 | myosin light chain kinase 2 | 5 | 6 | 2.2% | 0.00308 | 64685 |
[ |
IPI:IPI00122356.... | Ensembl_locations(Chr-bp):2-154606698 Myosin light chain kinase 2, skeletal/cardiac muscle | 5 | 6 | 2.1% | 0.003 | 65758 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 6 | 8 | 11.4% | 0.0029 | 93289 |
[ |
contaminant_NRL_... | owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... | 4 | 4 | 10.7% | 0.00285 | 46852 |
[ |
RPC40 | RNA polymerase subunit AC40, common to RNA polymerase I and III | 2 | 3 | 9.6% | 0.00274 | 37687 |
[ |
MRPS17 | Mitochondrial ribosomal protein of the small subunit | 2 | 2 | 10.5% | 0.00258 | 27635 |
[ |
RPS3 | Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... | 2 | 2 | 16.7% | 0.00255 | 26503 |
[ |
RPS2 | Protein component of the small (40S) subunit, essential for control of translational accuracy; phosp... | 2 | 2 | 13.0% | 0.00241 | 27450 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 4 | 4 | 12.8% | 0.00236 | 57749 |
[ |
CIR1 | Protein that interacts with frataxin (Yfh1p); putative homolog of mammalian electron transfer flavop... | 2 | 2 | 14.6% | 0.00234 | 28759 |
[ |
MRPL13 | Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation | 2 | 2 | 11.4% | 0.00231 | 30271 |
[ |
PAB1 | Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between th... | 4 | 4 | 11.3% | 0.00212 | 64344 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 3 | 3 | 11.9% | 0.0021 | 46914 |
[ |
PET9 | Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrial... | 2 | 2 | 9.7% | 0.00192 | 34426 |
[ |
KRT2 | keratin 2 | 4 | 4 | 6.2% | 0.00189 | 65865 |
[ |
ILV2 | Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is ... | 4 | 4 | 7.9% | 0.00178 | 74937 |
[ |
ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... | 2 | 2 | 7.7% | 0.00163 | 41690 |
[ |
RPL3 | Protein component of the large (60S) ribosomal subunit, has similarity to E. coli L3 and rat L3 ribo... | 2 | 2 | 5.4% | 0.00158 | 43758 |
[ |
TUB1 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 2 | 2 | 8.5% | 0.00137 | 49800 |
[ |
TUB3 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 2 | 2 | 8.5% | 0.00137 | 49694 |
[ |
LAT1 | Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... | 2 | 2 | 6.6% | 0.00127 | 51818 |
[ |
PGM1 | Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-ph... | 2 | 2 | 4.0% | 0.00107 | 63112 |
[ |
gi|2119236, gi|4... | sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... | 2 | 2 | 7.2% | 9.82E-4 | 61987 |
[ |
gi|17318569, gi|... | gi|39794653|gb|AAH63697.1| Keratin 1 [Homo sapiens], gi|17318569|ref|NP_006112.2| keratin 1 [Homo sa... | 2 | 2 | 7.9% | 9.49E-4 | 66067 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 2 | 2 | 6.0% | 8.52E-4 | 80047 |