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Bait Protein: DAD1
Organism: Saccharomyces cerevisiae
Publication: Shimogawa M. M. et al. (2006) Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase. Curr Biol. 2006 Aug 8;16(15):1489-501.
Comments: Dam1 complex purified from yeast with Dad1-TAP

Raw Data:

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UNFILTERED Run Results: [filter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   SPC19 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 10 96 67.3% 0.178 18910
[View Peptides]   ASK1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 19 42 63.0% 0.0439 32056
[View Peptides]   DAD1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 7 17 62.8% 0.0552 10516
[View Peptides]   TDH3 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... 26 41 59.9% 0.0377 35747
[View Peptides]   DAD3 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 7 13 59.6% 0.0422 10848
[View Peptides]   DUO1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 13 25 58.7% 0.0309 27473
[View Peptides]   RPS0B Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for mat... 11 15 51.6% 0.0182 27962
[View Peptides]   SPC34 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 16 41 51.2% 0.0425 34078
[View Peptides]   TDH2 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... 19 32 50.6% 0.0294 35847
[View Peptides]   DAM1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 19 36 44.6% 0.0321 38422
[View Peptides]   RPS0A Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for mat... 10 14 42.5% 0.017 28024
[View Peptides]   SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 17 21 41.4% 0.0105 66602
[View Peptides]   IPI:IPI00178254.... 5 7 40.3% 0.0101 22973
[View Peptides]   IPI:IPI00178276.... 5 7 40.3% 0.0101 23029
[View Peptides]   IPI:IPI00178198.... 5 7 39.5% 0.00995 23530
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 16 20 39.3% 0.00997 66595
[View Peptides]   IPI:IPI00178229.... 5 7 37.4% 0.00942 24762
[View Peptides]   gi|109502777 gi|109502777|ref|XP_001073968.1| PREDICTED: similar to calmodulin 1 [Rattus norvegicus] 3 3 36.9% 0.00615 16815
[View Peptides]   RPS7B Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Ap; interacts with K... 8 10 34.7% 0.0161 21634
[View Peptides]   SSA2 ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... 16 21 32.1% 0.01 69470
[View Peptides]   RPS14A Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processin... 4 5 32.1% 0.0111 14537
[View Peptides]   RPS14B Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processin... 4 5 31.9% 0.0111 14650
[View Peptides]   HSK3 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 3 4 30.4% 0.0177 8088
[View Peptides]   RPL31A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similari... 3 5 30.1% 0.0135 12953
[View Peptides]   RPL31B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similari... 3 5 30.1% 0.0135 12967
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 14 16 28.6% 0.00977 54545
[View Peptides]   RPL23B, RPL23A Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E... 6 13 28.5% 0.029 14473
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 14 19 27.9% 0.00904 69768
[View Peptides]   IGHG2 immunoglobulin heavy constant gamma 2 (G2m marker) 7 7 27.9% 0.00656 35885
[View Peptides]   RPL30 Protein component of the large (60S) ribosomal subunit, has similarity to rat L30 ribosomal protein;... 2 3 27.6% 0.00873 11415
[View Peptides]   DAD4 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 1 10 25.0% 0.0424 8155
[View Peptides]   CYS4 Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... 7 7 24.3% 0.00422 56022
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 6 7 24.1% 0.00592 39566
[View Peptides]   VMA4 Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 5 5 23.2% 0.00655 26471
[View Peptides]   CAPNS1 calpain, small subunit 1 4 6 23.1% 0.00684 28316
[View Peptides]   RPL14A N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... 3 4 21.7% 0.00885 15167
[View Peptides]   RPL14B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similari... 3 4 21.7% 0.00885 15153
[View Peptides]   RPL12B, RPL12A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... 2 2 20.6% 0.0037 17823
[View Peptides]   RPS7A Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with K... 6 7 20.0% 0.0113 21622
[View Peptides]   ATP3 Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily ... 5 6 19.9% 0.00589 34351
[View Peptides]   gi|119581084, gi... gi|40354192|ref|NP_000412.2| keratin 10 [Homo sapiens], gi|21961605|gb|AAH34697.1| Keratin 10 [Homo ... 6 7 19.0% 0.00366 58827
[View Peptides]   RPS19B Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... 3 5 18.8% 0.0106 15891
[View Peptides]   RPS19A Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... 3 5 18.8% 0.0106 15917
[View Peptides]   RPS1B Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarit... 4 5 18.4% 0.00599 28812
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 4 4 17.2% 0.00351 36849
[View Peptides]   RPS3 Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... 2 2 16.7% 0.00255 26503
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 8 10 15.8% 0.00333 99619
[View Peptides]   RPS5 Protein component of the small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal ... 4 7 15.6% 0.0095 25039
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 3 5 14.8% 0.00333 50033
[View Peptides]   CIR1 Protein that interacts with frataxin (Yfh1p); putative homolog of mammalian electron transfer flavop... 2 2 14.6% 0.00234 28759
[View Peptides]   RPS6A, RPS6B Protein component of the small (40S) ribosomal subunit; identical to Rps6Bp and has similarity to ra... 3 4 13.6% 0.00518 26996
[View Peptides]   RPS2 Protein component of the small (40S) subunit, essential for control of translational accuracy; phosp... 2 2 13.0% 0.00241 27450
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 4 4 12.8% 0.00236 57749
[View Peptides]   RPL15A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similari... 2 3 12.7% 0.00449 24422
[View Peptides]   RPL15B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Ap and has similari... 2 3 12.7% 0.00449 24422
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 3 3 11.9% 0.0021 46914
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 6 8 11.4% 0.0029 93289
[View Peptides]   MRPL13 Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation 2 2 11.4% 0.00231 30271
[View Peptides]   PAB1 Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between th... 4 4 11.3% 0.00212 64344
[View Peptides]   contaminant_NRL_... owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... 4 4 10.7% 0.00285 46852
[View Peptides]   MRPS17 Mitochondrial ribosomal protein of the small subunit 2 2 10.5% 0.00258 27635
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 3 33 9.9% 0.0278 42271
[View Peptides]   PET9 Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrial... 2 2 9.7% 0.00192 34426
[View Peptides]   RPC40 RNA polymerase subunit AC40, common to RNA polymerase I and III 2 3 9.6% 0.00274 37687
[View Peptides]   SSA3 ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotrans... 5 8 8.6% 0.00377 70547
[View Peptides]   TUB1 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... 2 2 8.5% 0.00137 49800
[View Peptides]   TUB3 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... 2 2 8.5% 0.00137 49694
[View Peptides]   ILV2 Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is ... 4 4 7.9% 0.00178 74937
[View Peptides]   gi|17318569, gi|... gi|39794653|gb|AAH63697.1| Keratin 1 [Homo sapiens], gi|17318569|ref|NP_006112.2| keratin 1 [Homo sa... 2 2 7.9% 9.49E-4 66067
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 2 2 7.7% 0.00163 41690
[View Peptides]   gi|2119236, gi|4... sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... 2 2 7.2% 9.82E-4 61987
[View Peptides]   LAT1 Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... 2 2 6.6% 0.00127 51818
[View Peptides]   KRT2 keratin 2 4 4 6.2% 0.00189 65865
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 2 2 6.0% 8.52E-4 80047
[View Peptides]   RPL3 Protein component of the large (60S) ribosomal subunit, has similarity to E. coli L3 and rat L3 ribo... 2 2 5.4% 0.00158 43758
[View Peptides]   PGM1 Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-ph... 2 2 4.0% 0.00107 63112
[View Peptides]   MYLK2 myosin light chain kinase 2 5 6 2.2% 0.00308 64685
[View Peptides]   IPI:IPI00122356.... Ensembl_locations(Chr-bp):2-154606698 Myosin light chain kinase 2, skeletal/cardiac muscle 5 6 2.1% 0.003 65758

YRC Informatics Platform - Version 3.0
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