Protein Overview: GCD1

General Information:
Name(s) found:	GCD1 / YOR260W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324834\|ref\|NP_014903.1\| Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; Gcd [NCBI NR] gi\|2506383\|sp\|P09032.3\|EI2BG_YEAST RecName: Full=Translation initiation factor eIF-2B subunit gamma; AltName: Full=eIF-2B GDP-GTP exchange factor subunit gamma; AltName: Full=Guanine nucleotide exchange factor subunit GCD1; AltName: Full=GCD complex subun [NCBI NR] pir\|\|BVBYD1 GCD1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1420587\|emb\|CAA99482.1\| GCD1 [Saccharomyces cerevisiae] [NCBI NR] Translation initiation factor eIF-2B subunit gamma OS=Saccharomyces cerevisiae GN=GCD1 PE=1 SV=3 [Swiss-Prot] (P09032) Translation initiation factor eIF-2B gamma subunit (eIF-2B GDP-GTP exchange factor) (Guanine nucleotide exchange factor subunit GCD1) (GCD complex subunit GCD1) [Swiss-Prot] GCD1 Translation initiation factor eIF2bgamma subunit [mips] Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression [SGD] Chr XV from 813982-815718 [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 813981-815717 [yeastorf3.fasta] GCD1, Chr XV from 813981-815717 [yeast_orfs2] GCD1 SGDID:S0005786, Chr XV from 813982-815718, Verified ORF [yeastflysequest.fasta] Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression [Saccharomyces cerevisiae]gi\|14 [nrdb_11152004_Chlamydomonas] GCD1 SGDID:S000005786, Chr XV from 813982-815718, Verified ORF, "Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negat [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] GCD1 SGDID:S000005786, Chr XV from 813984-815720, Verified ORF, "Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negat [yeast-contam.fasta] Translation initiation factor eIF-2B subunit gamma (eIF-2B GDP-GTP exchange factor subunit gamma) (Guanine nucleotide exchange factor subunit GCD1) (GCD complex subunit GCD1) [nr-271106-contam.fasta] Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; Gcd1p [Saccharomyces cerevisiae [nr-271106-contam.fasta] GCD1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GCD1 SGDID:S000005786, Chr XV from 813984-815720, Verified ORF, "Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Translation initiation factor eIF-2B subunit gamma OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GCD1 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GCD1 SGDID:S000005786, Chr XV from 813984-815720, Genome Release 64-1-1, Verified ORF, "Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression" [yeast-030211-contam.fasta] GCD1 SGDID:S000005786, Chr XV from 813984-815720, Genome Release 64-2-1, Verified ORF, "Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression" [20141208_orf_trans.fasta] YOR260W SGDID:S000005786, Chr XV from 813984-815720, Genome Release 64-1-1, Verified ORF, "Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	578 amino acids
Gene Ontology:
Cellular Component:	guanyl-nucleotide exchange factor complex [IDA ][IPI] eukaryotic translation initiation factor 2B complex [IDA]
Biological Process:	regulation of translational initiation [IDA][IGI][IMP ]
Molecular Function:	translation initiation factor activity [IMP ][IPI] guanyl-nucleotide exchange factor activity [IDA ][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSIQAFVFCG KGSNLAPFTQ PDFPFQTQNK DSTAATSGDK LNELVNSALD STVINEFMQH  60
   61 STRLPKALLP IGNRPMIEYV LDWCDQADFK EISVVAPVDE IELIESGLTS FLSLRKQQFE 120
  121 LIYKALSNSN HSHHLQDPKK INFIPSKANS TGESLQKELL PRINGDFVIL PCDFVTDIPP 180
  181 QVLVDQFRNR DDNNLAMTIY YKNSLDSSID KKQQQKQKQQ QFFTVYSENE DSERQPILLD 240
  241 VYSQRDVTKT KYLQIRSHLL WNYPNLTVST KLLNSFIYFC SFELCQLLKL GPQSMSRQAS 300
  301 FKDPFTGNQQ QQNPPTTDDD EDRNHDDDDD YKPSATSIQP TYFKKKNDLI LDPINCNKSL 360
  361 SKVFRDLSRR SWQHSKPREP IGIFILPNET LFIRANNLNA YMDANRFVLK IKSQTMFTKN 420
  421 IQIQSAAIGA DAIVDPKCQI SAHSNVKMSV LGTQANIGSR CRVAGSLLFP GVHLGDEVIL 480
  481 ENCIIGPMAK IGSKCKLSNC YIEGHYVVEP KNNFKGETLA NVYLDEDEED ELIYDDSVIA 540
  541 GESEIAEETD SDDRSDEDSD DSEYTDEYEY EDDGLFER

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CDC123 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 SUI2 SUI3
View Details	(MIPS) Mewes HW, et al. (2004)		GCD1 GCD2 GCD6 GCD7 GCN3
View Details	Krogan NJ, et al. (2006)		ADE1 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 MRF1 MRH4 SUI2
View Details	Gavin AC, et al. (2006)		GCD1 GCD11 GCD2 GCD6 GCD7 GCN3
View Details	Gavin AC, et al. (2002)		CCT4 CHC1 COG8 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 GUT1 IST1 PDC1 PRO1 RRP40 RRP46 SUI2 SUI3 XKS1
View Details	Ho Y, et al. (2002)		CDC123 CDC33 FAA4 FAL1 FET3 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 LOS1 MET18 NMD5 PRO3 SCW4 SUI2 SUI3 TIF1, TIF2 TIF4631 TIF4632 TUP1 VAC8 VMA8
View Details	Qiu et al. (2008)		GCD1 GCD2 GCD6 GCD7 GCN3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD10	GCD1	2	Uetz P, et al. (2000)
View Screen	RAD6	GCD1	1	Unpublished Fields Lab Data
View Screen	YPR097W	GCD1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GCD1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..345]	PSI-BLAST	84.154902	N-acetylglucosamine 1-phosphate uridyltransferase GlmU, C-terminal domain; N-acetylglucosamine 1-phosphate uridyltransferase GlmU, N-terminal domain
2	View Details	[346..578]	PSI-BLAST	84.154902	N-acetylglucosamine 1-phosphate uridyltransferase GlmU, C-terminal domain; N-acetylglucosamine 1-phosphate uridyltransferase GlmU, N-terminal domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
catalytic activity	1.88676710996045	bayes_pls_golite062009
nucleotidyltransferase activity	1.86990263440735	bayes_pls_golite062009
transferase activity	1.47184463413038	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.992215002450258	bayes_pls_golite062009
transferase activity, transferring hexosyl groups	0.796949457473955	bayes_pls_golite062009
acyltransferase activity	0.741838450922005	bayes_pls_golite062009
transferase activity, transferring acyl groups other than amino-acyl groups	0.717805296858439	bayes_pls_golite062009
UDP-glycosyltransferase activity	0.619458068514795	bayes_pls_golite062009
transferase activity, transferring acyl groups	0.555398952276587	bayes_pls_golite062009
binding	0.456682720062287	bayes_pls_golite062009
O-acyltransferase activity	0.319932817519902	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2