Protein Overview: FRA1

General Information:
Name(s) found:	FRA1 / YLL029W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74655019\|sp\|Q07825.1\|XPP_YEAST RecName: Full=Putative Xaa-Pro aminopeptidase [NCBI NR] gi\|6322999\|ref\|NP_013071.1\| Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL029W is not an essential gene but mutant is defective in spore formation; Rup2p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S64780 hypothetical protein YLL029w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1360212\|emb\|CAA97478.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Putative Xaa-Pro aminopeptidase OS=Saccharomyces cerevisiae GN=YLL029W PE=1 SV=1 [Swiss-Prot] Protein of unknown function localised to cytoplasm [mips] Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation [SGD] YLL029W, Chr XII from 81460-83709 [yeast_orfs2] Chr XII from 81460-83709 [yeastorf3.fasta] >Q07825 (Q07825) Chromosome XII reading frame ORF YLL029W [4932.FASTACCERV.txt] YLL029W SGDID:S0003952, Chr XII from 81460-83709, Uncharacterized ORF [yeastflysequest.fasta] Yll029wp [Saccharomyces cerevisiae]gi\|1360212\|emb\|CAA97478.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|2131729\|pir\|\|S64780 hypothetical protein YLL029w - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] (Q07825) S.cerevisiae chromosome XII reading frame ORF YLL029w [cerv4932.FASTAC] YLL029W SGDID:S000003952, Chr XII from 81460-83709, Uncharacterized ORF, "Hypothetical protein" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YLL029W SGDID:S000003952, Chr XII from 81460-83709, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL029W is not an essential gene but mutant is defective in spore fo [yeast-contam.fasta] FRA1 SGDID:S000003952, Chr XII from 81460-83709, Verified ORF, "Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of i [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL029W is not an essential gene but mutant is defective in spore formation; Yll029wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Putative Xaa-Pro aminopeptidase [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] FRA1 SGDID:S000003952, Chr XII from 81460-83709, Verified ORF, "Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation" [yeast-bovrabprots.fasta] YLL029W SGDID:S000003952, Chr XII from 81460-83709, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL029W is not an essential gene but mutant is defective in spore formation" [yeast-contam_custom.fasta] [chemostatdb.fasta] FRA1 SGDID:S000003952, Chr XII from 81461-83710, Genome Release 64-1-1, Verified ORF, "Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation" [yeast-030211-contam.fasta] FRA1 SGDID:S000003952, Chr XII from 81461-83710, Genome Release 64-2-1, Verified ORF, "Protein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation" [20141208_orf_trans.fasta] YLL029W SGDID:S000003952, Chr XII from 81461-83710, Genome Release 64-1-1, Verified ORF, "Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	749 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	biological_process [ND]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTSKPSTSDG RAHSISHVPG THMRGTSASH SPRPFRPCAD CTCSPGLLSR QGRRASLFLR  60
   61 QLENSRRSSS MLLNELKGAG GGSSAGNGSV YSCDSLCAVN REVNTTDRLL KLRQEMKKHD 120
  121 LCCYIVPSCD EHQSEYVSLR DQRRAFISGF SGSAGVACIT RDLLNFNDDH PDGKSILSTD 180
  181 GRYFNQARQE LDYNWTLLRQ NEDPITWQEW CVREALEMAK GLGNKEGMVL KIGIDPKLIT 240
  241 FNDYVSFRKM IDTKYDAKGK VELVPVEENL VDSIWPDFET LPERPCNDLL LLKYEFHGEE 300
  301 FKDKKEKLLK KLNDKASSAT TGRNTFIVVA LDEICWLLNL RGSDIDYNPV FFSYVAINED 360
  361 ETILFTNNPF NDDISEYFKI NGIEVRPYEQ IWEHLTKITS QASSAEHEFL IPDSASWQMV 420
  421 RCLNTSTNAN GAIAKKMTAQ NFAIIHSPID VLKSIKNDIE IKNAHKAQVK DAVCLVQYFA 480
  481 WLEQQLVGRE ALIDEYRAAE KLTEIRKTQR NFMGNSFETI SSTGSNAAII HYSPPVENSS 540
  541 MIDPTKIYLC DSGSQFLEGT TDITRTIHLT KPTKEEMDNY TLVLKGGLAL ERLIFPENTP 600
  601 GFNIDAIARQ FLWSRGLDYK HGTGHGIGSF LNVHEGPMGV GFRPHLMNFP LRAGNIISNE 660
  661 PGYYKDGEYG IRIESDMLIK KATEKGNFLK FENMTVVPYC RKLINTKLLN EEEKTQINEY 720
  721 HARVWRTIVH FLQPQSISYK WLKRETSPL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		FRA1 MET17 SIZ1
View Details	Ho Y, et al. (2002)		FRA1 FRA2 GRX3 GRX4

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	VAM3	sample: hx3	Hao Xu, et al. (2010)
View Run	VAM3	sample: hx4	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	VAM3	hx21: both fusion and trans-SNARE complex formation take place. Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)
View Run	VAM3	hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	LOS1	FRA1	1	Unpublished Fields Lab Data
View Screen	UBC7	FRA1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	FRA1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..83]	deduced	N/A	No confident structure predictions are available.
2	View Details	[84..277]	ab initio	8.2	No description for 1chmA was found.
3	View Details	[278..451]	PSI-BLAST	72.30103	AMINOPEPTIDASE P FROM E. COLI LOW PH FORM
4	View Details	[452..749]	PSI-BLAST	72.30103	AMINOPEPTIDASE P FROM E. COLI LOW PH FORM

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2