Protein Overview: ORC3

General Information:
Name(s) found:	ORC3 / YLL004W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323025\|ref\|NP_013097.1\| Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; Orc3p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S64746 ORC3 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1709489\|sp\|P54790.1\|ORC3_YEAST RecName: Full=Origin recognition complex subunit 3; AltName: Full=Origin recognition complex 62 kDa subunit [NCBI NR] gi\|1495220\|emb\|CAA62765.1\| L1365 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1360163\|emb\|CAA97447.1\| ORC3 [Saccharomyces cerevisiae] [NCBI NR] gi\|1297285\|gb\|AAC49270.1\| third subunit of the origin recognition complex Oaf1p/Orc3p [NCBI NR] gi\|1065913\|gb\|AAB38249.1\| Orc3p [Saccharomyces cerevisiae] [NCBI NR] Origin recognition complex subunit 3 OS=Saccharomyces cerevisiae GN=ORC3 PE=1 SV=1 [Swiss-Prot] ORC3 Origin recognition complex, 62 kDa subunit [mips] Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing [SGD] ORC3, Chr XII from 141072-142922 [yeast_orfs2] Chr XII from 141072-142922 [yeastorf3.fasta] (P54790) Origin recognition complex subunit 3 (Origin recognition complex protein 62 kDa subunit) [4932.FASTACCERV.txt] ORC3 SGDID:S0003927, Chr XII from 141072-142922, Verified ORF [yeastflysequest.fasta] Orc3p [Saccharomyces cerevisiae]gi\|1360163\|emb\|CAA97447.1\| ORC3 [Saccharomyces cerevisiae]gi\|1495220\|emb\|CAA62765.1\| L1365 protein [Saccharomyces cerevisiae]gi\|1709489\|sp\|P54790\|ORC3_YEAST Origin recognition complex subunit 3 (Origin recognition comple [nrdb_11152004_Chlamydomonas] ORC3 SGDID:S000003927, Chr XII from 141072-142922, Verified ORF, "Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Origin recognition complex subunit 3 (Origin recognition complex 62 kDa subunit) [nr-271106-contam.fasta] L1365 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ORC3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Orc3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; Orc3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] third subunit of the origin recognition complex Oaf1p/Orc3p [nr-271106-contam.fasta] [chemostatdb.fasta] Origin recognition complex subunit 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ORC3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ORC3 SGDID:S000003927, Chr XII from 141073-142923, Genome Release 64-1-1, Verified ORF, "Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing" [yeast-030211-contam.fasta] ORC3 SGDID:S000003927, Chr XII from 141073-142923, Genome Release 64-2-1, Verified ORF, "Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing" [20141208_orf_trans.fasta] YLL004W SGDID:S000003927, Chr XII from 141073-142923, Genome Release 64-1-1, Verified ORF, "Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	616 amino acids
Gene Ontology:
Cellular Component:	pre-replicative complex [TAS] nuclear origin of replication recognition complex [IDA][IMP]
Biological Process:	DNA replication initiation [TAS] pre-replicative complex assembly [TAS] chromatin silencing at silent mating-type cassette [TAS]
Molecular Function:	DNA replication origin binding [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDLNQSKKM NVSEFADAQR SHYTVYPSLP QSNKNDKHIP FVKLLSGKES EVNVEKRWEL  60
   61 YHQLHSHFHD QVDHIIDNIE ADLKAEISDL LYSETTQKRR CFNTIFLLGS DSTTKIELKD 120
  121 ESSRYNVLIE LTPKESPNVR MMLRRSMYKL YSAADAEEHP TIKYEDINDE DGDFTEQNND 180
  181 VSYDLSLVEN FKRLFGKDLA MVFNFKDVDS INFNTLDNFI ILLKSAFKYD HVKISLIFNI 240
  241 NTNLSNIEKN LRQSTIRLLK RNYHKLDVSS NKGFKYGNQI FQSFLDTVDG KLNLSDRFVE 300
  301 FILSKMANNT NHNLQLLTKM LDYSLMSYFF QNAFSVFIDP VNVDFLNDDY LKILSRCPTF 360
  361 MFFVEGLIKQ HAPADEILSL LTNKNRGLEE FFVEFLVREN PINGHAKFVA RFLEEELNIT 420
  421 NFNLIELYHN LLIGKLDSYL DRWSACKEYK DRLHFEPIDT IFQELFTLDN RSGLLTQSIF 480
  481 PSYKSNIEDN LLSWEQVLPS LDKENYDTLS GDLDKIMAPV LGQLFKLYRE ANMTINIYDF 540
  541 YIAFRETLPK EEILNFIRKD PSNTKLLELA ETPDAFDKVA LILFMQAIFA FENMGLIKFQ 600
  601 STKSYDLVEK CVWRGI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		HAT1 HAT2 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6
View Details	(MIPS) Mewes HW, et al. (2004)		CDC45 CDC6 CDC7 DBF4 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32 SLD3
View Details	Krogan NJ, et al. (2006)		HAT1 HAT2 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 SWE1 YJR154W
View Details	Gavin AC, et al. (2006)		ORC1 ORC2 ORC3 ORC4 ORC5 ORC6
View Details	Gavin AC, et al. (2002)		CDC45 ERG26 GLT1 HAT1 HSP42 LYS12 MCM2 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 RPD3 RPN9 SIN3 TFP1
View Details	Qiu et al. (2008)		CDC45 CDC6 CDC7 DBF4 DPB11 DPB2 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32 SLD3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ORC3	SUA5	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..122]	deduced	N/A	No confident structure predictions are available.
2	View Details	[123..144] [156..289]	ORFEUS	7.56	(+)-bornyl diphosphate synthase
3	View Details	[145..155] [290..506]	ORFEUS	7.56	(+)-bornyl diphosphate synthase
4	View Details	[507..616]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2