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Protein Overview: PUF4

General Information:
Name(s) found: PUF4 / YGL014W [SGD]
Description(s) found:
Found 24 descriptions.
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  • Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 888 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed article]
    Biological Process: loss of chromatin silencing involved in replicative cell aging [IGILink to PubMed article][IMPLink to PubMed article]
    protein localization [IMPLink to PubMed article]
    mRNA catabolic process [IEPLink to PubMed article]
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IGILink to PubMed article]
    Molecular Function: mRNA binding [IDALink to PubMed article][ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTKGLKEEI DDVPSVDPVV SETVNSALEQ LQLDDPEENA TSNAFANKVS QDSQFANGPP  60
   61 SQMFPHPQMM GGMGFMPYSQ MMQVPHNPCP FFPPPDFNDP TAPLSSSPLN AGGPPMLFKN 120
  121 DSLPFQMLSS GAAVATQGGQ NLNPLINDNS MKVLPIASAD PLWTHSNVPG SASVAIEETT 180
  181 ATLQESLPSK GRESNNKASS FRRQTFHALS PTDLINAANN VTLSKDFQSD MQNFSKAKKP 240
  241 SVGANNTAKT RTQSISFDNT PSSTSFIPPT NSVSEKLSDF KIETSKEDLI NKTAPAKKES 300
  301 PTTYGAAYPY GGPLLQPNPI MPGHPHNISS PIYGIRSPFP NSYEMGAQFQ PFSPILNPTS 360
  361 HSLNANSPIP LTQSPIHLAP VLNPSSNSVA FSDMKNDGGK PTTDNDKAGP NVRMDLINPN 420
  421 LGPSMQPFHI LPPQQNTPPP PWLYSTPPPF NAMVPPHLLA QNHMPLMNSA NNKHHGRNNN 480
  481 SMSSHNDNDN IGNSNYNNKD TGRSNVGKMK NMKNSYHGYY NNNNNNNNNN NNNNNSNATN 540
  541 SNSAEKQRKI EESSRFADAV LDQYIGSIHS LCKDQHGCRF LQKQLDILGS KAADAIFEET 600
  601 KDYTVELMTD SFGNYLIQKL LEEVTTEQRI VLTKISSPHF VEISLNPHGT RALQKLIECI 660
  661 KTDEEAQIVV DSLRPYTVQL SKDLNGNHVI QKCLQRLKPE NFQFIFDAIS DSCIDIATHR 720
  721 HGCCVLQRCL DHGTTEQCDN LCDKLLALVD KLTLDPFGNY VVQYIITKEA EKNKYDYTHK 780
  781 IVHLLKPRAI ELSIHKFGSN VIEKILKTAI VSEPMILEIL NNGGETGIQS LLNDSYGNYV 840
  841 LQTALDISHK QNDYLYKRLS EIVAPLLVGP IRNTPHGKRI IGMLHLDS

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ISA2 NHP6A PBP4 PUF4 RPL11B RPL12B, RPL12A RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL1A, RPL1B RPL20A RPL21A RPL24A RPL29 RPL2A, RPL2B RPL3 RPL30 RPL32 RPL34A RPL36A RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL6A RPL8B RPL9A RPS11A, RPS11B RPS14A RPS16B, RPS16A RPS17B RPS1A RPS2 RPS22A RPS23B, RPS23A RPS26B RPS3 RPS30B, RPS30A RPS5 RPS8B, RPS8A RPS9B YHI9 YLL023C
    View Details Krogan NJ, et al. (2006) BNA1 PUF4

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen SEC17 PUF4 1 Unpublished Fields Lab Data
    View Screen GAL7 PUF4 1 Unpublished Fields Lab Data
    View Screen SLY1 PUF4 1 Unpublished Fields Lab Data
    View Screen UBC6 PUF4 1 Unpublished Fields Lab Data
    View Screen SPT2 PUF4 1 Unpublished Fields Lab Data
    View Screen SPT23 PUF4 1 Unpublished Fields Lab Data
    View Screen RRB1 PUF4 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data PUF4 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..89] deduced N/A No confident structure predictions are available.
    2 View Details [90..216] PSI-BLAST 3.09691 Fibrinogen
    3 View Details [217..446] ab initio 11.96 No description for 1deqA was found.
    4 View Details [447..521] deduced N/A No confident structure predictions are available.
    5 View Details [522..673] PSI-BLAST 624.228787 Pumilio 1
    6 View Details [674..776] PSI-BLAST 624.228787 Pumilio 1
    7 View Details [777..888] PSI-BLAST 624.228787 Pumilio 1


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle