Protein Overview: LCB2

General Information:
Name(s) found:	LCB2 / YDR062W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|798913\|emb\|CAA89091.1\| Lcb2p [Saccharomyces cerevisiae] [NCBI NR] gi\|729923\|sp\|P40970.1\|LCB2_YEAST RecName: Full=Serine palmitoyltransferase 2; Short=SPT 2; AltName: Full=Long chain base biosynthesis protein 2 [NCBI NR] gi\|706828\|emb\|CAA58978.1\| subunit of serine palmitoyl transferase [Saccharomyces cerevisiae] [NCBI NR] gi\|6320267\|ref\|NP_010347.1\| Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine; Lcb2p [Saccharomyc [NCBI NR] gi\|536871\|gb\|AAA34740.1\| serine palmitoyl transferase [Saccharomyces cerevisiae] [NCBI NR] gi\|1431513\|emb\|CAA98880.1\| LCB2 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S54046 serine C-palmitoyltransferase (EC 2.3.1.50) chain LCB2 - yeast (Saccharomyces cerevisiae) [NCBI NR] Serine palmitoyltransferase 2 OS=Saccharomyces cerevisiae GN=LCB2 PE=1 SV=1 [Swiss-Prot] LCB2 Serine C-palmitoyltransferase subunit [mips] Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine [SGD] LCB2, Chr IV from 576468-578153 [yeast_orfs2] Chr IV from 576468-578153 [yeastorf3.fasta] (P40970) Serine palmitoyltransferase 2 (EC 2.3.1.50) (Long chain base biosynthesis protein 2) (SPT 2) [4932.FASTACCERV.txt] LCB2 SGDID:S0002469, Chr IV from 576469-578154, Verified ORF [yeastflysequest.fasta] LCB2 SGDID:S0002469, Chr IV from 576470-578155, Verified ORF [10112005cervSGD.fasta] Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine [Saccharomyces cerevisiae]gi\|1431513\|emb\|CAA98 [nrdb_11152004_Chlamydomonas] LCB2 SGDID:S000002469, Chr IV from 576470-578155, Verified ORF, "Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] LCB2 SGDID:S000002469, Chr IV from 576471-578156, Verified ORF, "Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form [yeast-contam.fasta] LCB2 SGDID:S000002469, Chr IV from 576472-578157, Verified ORF, "Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Serine palmitoyltransferase 2 (Long chain base biosynthesis protein 2) (SPT 2) [nr-271106-contam.fasta] LCB2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine; Lcb2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] serine palmitoyl transferase [nr-271106-contam.fasta] Lcb2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] subunit of serine palmitoyl transferase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] LCB2 SGDID:S000002469, Chr IV from 576472-578157, Verified ORF, "Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine" [yeast-bovrabprots.fasta] LCB2 SGDID:S000002469, Chr IV from 576471-578156, Verified ORF, "Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine" [yeast-contam_custom.fasta] [chemostatdb.fasta] Serine palmitoyltransferase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LCB2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] LCB2 SGDID:S000002469, Chr IV from 576474-578159, Genome Release 64-1-1, Verified ORF, "Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine" [yeast-030211-contam.fasta] LCB2 SGDID:S000002469, Chr IV from 576474-578159, Genome Release 64-2-1, Verified ORF, "Component of serine palmitoyltransferase; responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine" [20141208_orf_trans.fasta] YDR062W SGDID:S000002469, Chr IV from 576474-578159, Genome Release 64-1-1, Verified ORF, "Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	561 amino acids
Gene Ontology:
Cellular Component:	membrane fraction [IDA] microsome [IPI] serine C-palmitoyltransferase complex [IMP]
Biological Process:	sphingolipid biosynthetic process [TAS]
Molecular Function:	serine C-palmitoyltransferase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTPANYTRV PLCEPEELPD DIQKENEYGT LDSPGHLYQV KSRHGKPLPE PVVDTPPYYI  60
   61 SLLTYLNYLI LIILGHVHDF LGMTFQKNKH LDLLEHDGLA PWFSNFESFY VRRIKMRIDD 120
  121 CFSRPTTGVP GRFIRCIDRI SHNINEYFTY SGAVYPCMNL SSYNYLGFAQ SKGQCTDAAL 180
  181 ESVDKYSIQS GGPRAQIGTT DLHIKAEKLV ARFIGKEDAL VFSMGYGTNA NLFNAFLDKK 240
  241 CLVISDELNH TSIRTGVRLS GAAVRTFKHG DMVGLEKLIR EQIVLGQPKT NRPWKKILIC 300
  301 AEGLFSMEGT LCNLPKLVEL KKKYKCYLFI DEAHSIGAMG PTGRGVCEIF GVDPKDVDIL 360
  361 MGTFTKSFGA AGGYIAADQW IIDRLRLDLT TVSYSESMPA PVLAQTISSL QTISGEICPG 420
  421 QGTERLQRIA FNSRYLRLAL QRLGFIVYGV ADSPVIPLLL YCPSKMPAFS RMMLQRRIAV 480
  481 VVVAYPATPL IESRVRFCMS ASLTKEDIDY LLRHVSEVGD KLNLKSNSGK SSYDGKRQRW 540
  541 DIEEVIRRTP EDCKDDKYFV N

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		LCB1 LCB2
View Details	Gavin AC, et al. (2006)		LCB1 LCB2
View Details	Gavin AC, et al. (2002)		BMH1 BMH2 CLU1 CRM1 ECM29 FKS1 GCN1 GEA2 GFA1 HRP1 KAP104 KAP123 LCB1 LCB2 LYS12 MIR1 NAB2 SAM1 SAM2 YHR020W
View Details	Qiu et al. (2008)		LCB1 LCB2 PIS1 SUR4

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	LCB2	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..99]	deduced	N/A	No confident structure predictions are available.
2	View Details	[100..163] [428..561]	PSI-BLAST	499.325697	2-amino-3-ketobutyrate CoA ligase
3	View Details	[164..427]	PSI-BLAST	499.325697	2-amino-3-ketobutyrate CoA ligase

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.48

Protein Overview: LCB2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: