YRC Public Data Repository - Protein Structure Prediction

Protein:	TPK1
Organism:	Saccharomyces cerevisiae
Length:	397 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for TPK1.

Description	E-value	Query Range	Subject Range
TPK1 - cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients... KAPA_YEAST - cAMP-dependent protein kinase type 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TPK...	0.0	[1..397]	[1..397]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[1..397]	[3..399]
gi\|4322298 - gi\|4322298\|gb\|AAD16003.1\| cAMP-dependent protein kinase catalytic subunit isoform 2 [Amblyomma ameri...	0.0	[2..397]	[26..421]
gi\|8489808 - gi\|8489808\|gb\|AAF75762.1\|AF262987_1 cAMP-dependent protein kinase PKAC catalytic subunit [Emericella... gi\|67540270, gi\|... - gi\|67540270\|ref\|XP_663909.1\| hypothetical protein AN6305.2 [Aspergillus nidulans FGSC A4], ref\|XP_41...	0.0	[4..397]	[78..472]
gi\|16648134 - gi\|16648134\|gb\|AAL25332.1\| GH13631p [Drosophila melanogaster]	0.0	[1..397]	[132..540]
gi\|12005625 - gi\|12005625\|gb\|AAG44542.1\|AF247001_1 protein kinase C [Blumeria graminis]	0.0	[2..397]	[746..1150]

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Predicted Domain #1

Region A:
Residues: [1-60]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE TQEKPKQPHV  60
   61

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
cyclic nucleotide-dependent protein kinase activity	4.97855712164511	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
cAMP-dependent protein kinase activity	4.66277812893755	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
protein tyrosine kinase activity	2.8153155526437	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
transmembrane receptor activity	2.59526003830791	bayes_pls_golite062009
transmembrane receptor protein kinase activity	2.54265774272269	bayes_pls_golite062009
molecular transducer activity	2.40586892801446	bayes_pls_golite062009
signal transducer activity	2.40586892801446	bayes_pls_golite062009
receptor activity	2.39311927684508	bayes_pls_golite062009
tubulin binding	2.18641675163967	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase activity	2.16831387024372	bayes_pls_golite062009
cytoskeletal protein binding	2.12615078788254	bayes_pls_golite062009
receptor signaling protein activity	2.10379226653669	bayes_pls_golite062009
tau-protein kinase activity	2.00481226596005	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
microtubule binding	1.88110801192607	bayes_pls_golite062009
actin binding	1.87817875565699	bayes_pls_golite062009
acetylcholine receptor activator activity	1.83545227594542	bayes_pls_golite062009
phosphoinositide 3-kinase binding	1.82515074380651	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.81249386965144	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
insulin receptor substrate binding	1.62657794525303	bayes_pls_golite062009
transcription regulator activity	1.56748951434625	bayes_pls_golite062009
JUN kinase kinase kinase kinase activity	1.55257528159541	bayes_pls_golite062009
DNA binding	1.35333135345112	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.32254739984688	bayes_pls_golite062009
gamma-tubulin binding	1.28511591054866	bayes_pls_golite062009
kinase binding	1.26086833316556	bayes_pls_golite062009
ErbB-3 class receptor binding	1.25765701903393	bayes_pls_golite062009
protein binding	1.22565748038754	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
nucleic acid binding	1.16049201286103	bayes_pls_golite062009
transporter activity	1.11833481501707	bayes_pls_golite062009
transcription factor activity	1.10928254946881	bayes_pls_golite062009
protein kinase binding	1.06030596516506	bayes_pls_golite062009
ErbB-2 class receptor binding	1.02996109010965	bayes_pls_golite062009
transmembrane transporter activity	0.97575992765505	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.959360414036299	bayes_pls_golite062009
growth factor binding	0.86076618871384	bayes_pls_golite062009
MAP kinase activity	0.81943579965027	bayes_pls_golite062009
actin filament binding	0.794152600084	bayes_pls_golite062009
substrate-specific transporter activity	0.705905455086486	bayes_pls_golite062009
structural constituent of cytoskeleton	0.612883428421652	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
enzyme binding	0.46284745266176	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.450699332958421	bayes_pls_golite062009
protein kinase C activity	0.41587987561486	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
MAP kinase kinase activity	0.35402045013148	bayes_pls_golite062009
insulin receptor binding	0.266450215230107	bayes_pls_golite062009
protein complex binding	0.239339759110451	bayes_pls_golite062009
insulin receptor activity	0.237134032032089	bayes_pls_golite062009
SH2 domain binding	0.226760162354251	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase activity	0.20643152368029	bayes_pls_golite062009
transforming growth factor beta receptor activity	0.20643152368029	bayes_pls_golite062009
receptor binding	0.197402253061255	bayes_pls_golite062009
ion transmembrane transporter activity	0.148194101970458	bayes_pls_golite062009
peptide binding	0.118578378753757	bayes_pls_golite062009
transforming growth factor beta receptor activity, type I	0.0506751594607393	bayes_pls_golite062009
MAP kinase kinase kinase kinase activity	0.01826424351081	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [61-136]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 TYYNEEQYKQ FIAQARVTVG SIVYKNFQIL RTLGTGSFGR VHLIRSRHNG RYYAMKVLKK  60
   61 EIVVRLKQVE HTNDER

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [150-166]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MWGTFQDAQQ IFMIMDY

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [362-397]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 IQQGQGDTSQ FDKYPEEDIN YGVQGEDPYA DLFRDF

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	10140.0
Match:	1fotA_
Description:	cAMP-dependent PK, catalytic subunit
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [137-149]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LMLSIVTHPF IIR

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [167-361]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 IEGGELFSLL RKSQRFPNPV AKFYAAEVCL ALEYLHSKDI IYRDLKPENI LLDKNGHIKI  60
   61 TDFGFAKYVP DVTYTLCGTP DYIAPEVVST KPYNKSIDWW SFGILIYEML AGYTPFYDSN 120
  121 TMKTYEKILN AELRFPPFFN EDVKDLLSRL ITRDLSQRLG NLQNGTEDVK NHPWFKEVVW 180
  181 EKLLSRNIET PYEPP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	10140.0
Match:	1fotA_
Description:	cAMP-dependent PK, catalytic subunit
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: