Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
ACC1 | Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... | 2 | 2 | 1.9% | 1.9E-4 | 250351 |
[ |
ADE5,7 | Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole... | 2 | 2 | 5.1% | 5.3E-4 | 86068 |
[ |
ARB1 | ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has sim... | 2 | 2 | 5.2% | 6.96E-4 | 68377 |
[ |
ARF1 | ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle form... | 2 | 7 | 9.9% | 0.00821 | 20529 |
[ |
ARO2 | Bifunctional chorismate synthase and flavin reductase, catalyzes the conversion of 5-enolpyruvylshik... | 2 | 3 | 4.3% | 0.00169 | 40838 |
[ |
ASC1 | G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... | 6 | 11 | 29.5% | 0.00732 | 34805 |
[ |
ASK10 | Component of the RNA polymerase II holoenzyme, phosphorylated in response to oxidative stress; has a... | 2 | 2 | 4.5% | 3.71E-4 | 126864 |
[ |
BAG7 | Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which pla... | 2 | 2 | 12.0% | 0.00104 | 46216 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 5 | 5 | 15.1% | 0.00276 | 45384 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 15 | 29 | 40.1% | 0.0107 | 66087 |
[ |
CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... | 6 | 18 | 5.3% | 0.00694 | 61907 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 10 | 22 | 31.6% | 0.00934 | 54545 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 9 | 11 | 62.1% | 0.0145 | 18751 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 4 | 11 | 66.0% | 0.0159 | 16135 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 15 | 34 | 34.9% | 0.0124 | 67400 |
[ |
CWP1 | Cell wall mannoprotein that localizes specifically to birth scars of daughter cells, linked to a bet... | 2 | 7 | 13.4% | 0.00622 | 24268 |
[ |
CYS3 | Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathw... | 2 | 9 | 6.1% | 0.00485 | 42542 |
[ |
DBP9 | ATP-dependent RNA helicase of the DEAD-box family involved in biogenesis of the 60S ribosomal subuni... | 2 | 3 | 5.7% | 0.00107 | 68060 |
[ |
DED81 | Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attach... | 2 | 11 | 5.6% | 0.00422 | 62207 |
[ |
DNA2 | Tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease,... | 2 | 2 | 3.3% | 2.79E-4 | 171694 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 3 | 10 | 6.1% | 0.00252 | 93289 |
[ |
ENO1 | Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... | 8 | 31 | 19.7% | 0.0151 | 46816 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 11 | 40 | 25.2% | 0.0194 | 46914 |
[ |
ESC1 | Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-dom... | 2 | 2 | 1.7% | 2.56E-4 | 187137 |
[ |
FAS1 | Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty a... | 2 | 2 | 1.8% | 2.07E-4 | 228689 |
[ |
FBA1 | Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... | 7 | 22 | 46.8% | 0.013 | 39621 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 4 | 6 | 9.2% | 0.00178 | 80047 |
[ |
GUS1 | Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; ... | 3 | 3 | 8.5% | 9.0E-4 | 80843 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 5 | 20 | 9.6% | 0.00602 | 80900 |
[ |
HSP42 | Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress... | 2 | 3 | 6.7% | 0.0017 | 42817 |
[ |
HSP82 | Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p... | 5 | 20 | 9.6% | 0.00599 | 81406 |
[ |
HTA1 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 2 | 3 | 44.7% | 0.00483 | 13989 |
[ |
HTA2 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 2 | 3 | 44.7% | 0.00483 | 13989 |
[ |
KAR1 | Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... | 4 | 8 | 12.7% | 0.00392 | 50653 |
[ |
KRI1 | Essential nucleolar protein required for 40S ribosome biogenesis; physically and functionally intera... | 2 | 2 | 9.1% | 7.19E-4 | 68654 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 2 | 2 | 2.7% | 2.27E-4 | 218454 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 80 | 252 | 49.9% | 0.0319 | 195140 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 3 | 18 | 9.8% | 0.00988 | 44585 |
[ |
NIP1 | eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of ... | 2 | 3 | 7.5% | 7.85E-4 | 93204 |
[ |
NOP2 | Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and la... | 2 | 9 | 8.4% | 0.00309 | 69812 |
[ |
NSR1 | Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... | 2 | 2 | 11.4% | 0.00103 | 44535 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 25 | 81 | 24.4% | 0.0202 | 94104 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 3 | 7 | 11.5% | 0.00264 | 61495 |
[ |
PFK1 | Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaer... | 2 | 5 | 2.8% | 0.00108 | 107970 |
[ |
PGK1 | 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... | 2 | 2 | 5.3% | 0.00102 | 44738 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 10 | 15 | 17.4% | 0.00347 | 99619 |
[ |
PSA1 | GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... | 5 | 12 | 19.9% | 0.00706 | 39566 |
[ |
RHR2 | Constitutively expressed isoform of DL-glycerol-3-phosphatase; involved in glycerol biosynthesis, in... | 2 | 4 | 10.0% | 0.00313 | 30439 |
[ |
RPN3 | Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of th... | 2 | 8 | 5.5% | 0.00325 | 60393 |
[ |
RPP1B | Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of tra... | 2 | 6 | 67.0% | 0.012 | 10668 |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 2 | 3 | 23.6% | 0.00601 | 10746 |
[ |
SFI1 | Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the ... | 2 | 3 | 3.5% | 6.73E-4 | 112978 |
[ |
SLX5 | Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified... | 2 | 2 | 8.1% | 6.86E-4 | 71071 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 36 | 79 | 38.3% | 0.0178 | 111782 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 26 | 72 | 56.9% | 0.0604 | 29280 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 39 | 107 | 57.6% | 0.0626 | 42271 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 4 | 7 | 9.3% | 0.00239 | 72105 |
[ |
STU2 | Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics duri... | 2 | 2 | 6.9% | 4.78E-4 | 100918 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 3 | 5 | 9.6% | 0.00232 | 50033 |
[ |
TUB1 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 2 | 3 | 6.3% | 0.00143 | 49800 |
[ |
TUB3 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 2 | 3 | 6.3% | 0.00143 | 49694 |
[ |
UBP9 | Ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fu... | 2 | 2 | 7.8% | 5.63E-4 | 86244 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 2 | 5 | 2.7% | 0.00205 | 57749 |
[ |
YBL005W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 8 | 3.1% | 9.68E-4 | 198968 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 7 | 20 | 28.6% | 0.0104 | 44671 |
[ |
YDR261C-D | Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional | 3 | 8 | 3.4% | 0.00106 | 181660 |
[ |
YDR365W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 8 | 3.1% | 9.68E-4 | 198658 |
[ |
YEF3 | Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... | 5 | 13 | 11.2% | 0.00264 | 115945 |
[ |
YGR027W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 8 | 3.1% | 9.68E-4 | 198656 |
[ |
YGR109W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 2.8% | 2.75E-4 | 178419 |
[ |
YGR161C-D | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 8 | 3.1% | 9.68E-4 | 198558 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 11 | 41 | 98.6% | 0.126 | 7729 |
[ |
YJR029W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 8 | 3.1% | 9.68E-4 | 198616 |
[ |
YLR146W-A | Putative protein of unknown function | 2 | 2 | 33.9% | 0.00685 | 7078 |
[ |
YLR157C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 8 | 3.1% | 9.68E-4 | 198544 |
[ |
YLR227W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 8 | 3.1% | 9.68E-4 | 198404 |
[ |
YOR051C | Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model s... | 2 | 2 | 13.8% | 0.00103 | 47352 |
[ |
YRA1 | RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA e... | 2 | 2 | 7.1% | 0.00188 | 24956 |