Protein Overview: VPS8

General Information:
Name(s) found:	VPS8 / YAL002W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|50403741\|sp\|P39702.2\|VPS8_YEAST RecName: Full=Vacuolar protein sorting-associated protein 8; AltName: Full=Vacuolar protein-targeting protein 8 [NCBI NR] gi\|41629668\|ref\|NP_009399.2\| Membrane-associated protein that interacts with Vps21p to facilitate soluble vacuolar protein localization; component of the CORVET complex; required for localization and trafficking of the CPY sorting receptor; contains RING [NCBI NR] gi\|41387231\|gb\|AAC04955.2\| Vps8p [Saccharomyces cerevisiae] [NCBI NR] Vacuolar protein sorting-associated protein 8 OS=Saccharomyces cerevisiae GN=VPS8 PE=1 SV=2 [Swiss-Prot] VPS8 Vacuolar sorting protein, 134 kD [mips] Membrane-associated protein that interacts with Vps21p to facilitate soluble vacuolar protein localization; component of the CORVET complex; required for localization and trafficking of the CPY sorting receptor; contains RING finger motif [SGD] VPS8 SGDID:S0000002, Chr I from 143711-147535, Verified ORF [yeastflysequest.fasta] VPS8 SGDID:S0000002, Chr I from 143709-147533, Verified ORF [10112005cervSGD.fasta] Vps8p [Saccharomyces cerevisiae]gi\|41387231\|gb\|AAC04955.2\| Vps8p [Saccharomyces cerevisiae]gi\|50403741\|sp\|P39702\|VPS8_YEAST Vacuolar protein sorting-associated protein VPS8 [nrdb_11152004_Chlamydomonas] (P39702) Vacuolar protein sorting-associated protein VPS8 [cerv4932.FASTAC] VPS8 SGDID:S000000002, Chr I from 143709-147533, Verified ORF, "Membrane-associated hydrophilic protein that interacts with the small GTPase, Vps21p, to facilitate soluble vacuolar protein localization; required for localization and trafficking of the CPY [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] VPS8 SGDID:S000000002, Chr I from 143709-147533, Verified ORF, "Membrane-associated protein that interacts with Vps21p to facilitate soluble vacuolar protein localization; component of the CORVET complex; required for localization and trafficking of the C [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Vps8p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Vacuolar protein sorting-associated protein 8 (Vacuolar protein-targeting protein 8) [nr-271106-contam.fasta] Membrane-associated hydrophilic protein that interacts with the small GTPase, Vps21p, to facilitate soluble vacuolar protein localization; required for localization and trafficking of the CPY sorting receptor; contains a RING finger motif; Vps8p [Saccharo [nr-271106-contam.fasta] VPS8 SGDID:S000000002, Chr I from 143709-147533, Verified ORF, "Membrane-associated protein that interacts with Vps21p to facilitate soluble vacuolar protein localization; component of the CORVET complex; required for localization and trafficking of the CPY sorting receptor; contains RING finger motif" [yeast-bovrabprots.fasta] VPS8 SGDID:S000000002, Chr I from 143709-147533, Verified ORF, "Membrane-associated hydrophilic protein that interacts with the small GTPase, Vps21p, to facilitate soluble vacuolar protein localization; required for localization and trafficking of the CPY sorting receptor; contains a RING finger motif" [yeast-contam_custom.fasta] [chemostatdb.fasta] Vacuolar protein sorting-associated protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=VPS8 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] VPS8 SGDID:S000000002, Chr I from 143707-147531, Genome Release 64-1-1, Verified ORF, "Membrane-associated protein that interacts with Vps21p to facilitate soluble vacuolar protein localization; component of the CORVET complex; required for localization and trafficking of the CPY sorting receptor; contains RING finger motif" [yeast-030211-contam.fasta] VPS8 SGDID:S000000002, Chr I from 143707-147531, Genome Release 64-2-1, Verified ORF, "Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif" [20141208_orf_trans.fasta] YAL002W SGDID:S000000002, Chr I from 143707-147531, Genome Release 64-1-1, Verified ORF, "Membrane-associated protein that interacts with Vps21p to facilitate soluble vacuolar protein localization; component of the CORVET complex; required for localization and trafficking of the CPY sorting receptor; contains RING finger motif" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1274 amino acids
Gene Ontology:
Cellular Component:	membrane fraction [IDA]
Biological Process:	late endosome to vacuole transport [IGI][IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEQNGLDHDS RSSIDTTIND TQKTFLEFRS YTQLSEKLAS SSSYTAPPLN EDGPKGVASA  60
   61 VSQGSESVVS WTTLTHVYSI LGAYGGPTCL YPTATYFLMG TSKGCVLIFN YNEHLQTILV 120
  121 PTLSEDPSIH SIRSPVKSIV ICSDGTHVAA SYETGNICIW NLNVGYRVKP TSEPTNGMTP 180
  181 TPALPAVLHI DDHVNKEITG LDFFGARHTA LIVSDRTGKV SLYNGYRRGF WQLVYNSKKI 240
  241 LDVNSSKEKL IRSKLSPLIS REKISTNLLS VLTTTHFALI LLSPHVSLMF QETVEPSVQN 300
  301 SLVVNSSISW TQNCSRVAYS VNNKISVISI SSSDFNVQSA SHSPEFAESI LSIQWIDQLL 360
  361 LGVLTISHQF LVLHPQHDFK ILLRLDFLIH DLMIPPNKYF VISRRSFYLL TNYSFKIGKF 420
  421 VSWSDITLRH ILKGDYLGAL EFIESLLQPY CPLANLLKLD NNTEERTKQL MEPFYNLSLA 480
  481 ALRFLIKKDN ADYNRVYQLL MVVVRVLQQS SKKLDSIPSL DVFLEQGLEF FELKDNAVYF 540
  541 EVVANIVAQG SVTSISPVLF RSIIDYYAKE ENLKVIEDLI IMLNPTTLDV DLAVKLCQKY 600
  601 NLFDLLIYIW NKIFDDYQTP VVDLIYRISN QSEKCVIFNG PQVPPETTIF DYVTYILTGR 660
  661 QYPQNLSISP SDKCSKIQRE LSAFIFSGFS IKWPSNSNHK LYICENPEEE PAFPYFHLLL 720
  721 KSNPSRFLAM LNEVFEASLF NDDNDMVASV GEAELVSRQY VIDLLLDAMK DTGNSDNIRV 780
  781 LVAIFIATSI SKYPQFIKVS NQALDCVVNT ICSSRVQGIY EISQIALESL LPYYHSRTTE 840
  841 NFILELKEKN FNKVLFHIYK SENKYASALS LILETKDIEK EYNTDIVSIT DYILKKCPPG 900
  901 SLECGKVTEV IETNFDLLLS RIGIEKCVTI FSDFDYNLHQ EILEVKNEET QQKYLDKLFS 960
  961 TPNINNKVDK RLRNLHIELN CKYKSKREMI LWLNGTVLSN AESLQILDLL NQDSNFEAAA1020
 1021 IIHERLESFN LAVRDLLSFI EQCLNEGKTN ISTLLESLRR AFDDCNSAGT EKKSCWILLI1080
 1081 TFLITLYGKY PSHDERKDLC NKLLQEAFLG LVRSKSSSQK DSGGEFWEIM SSVLEHQDVI1140
 1141 LMKVQDLKQL LLNVFNTYKL ERSLSELIQK IIEDSSQDLV QQYRKFLSEG WSIHTDDCEI1200
 1201 CGKKIWGAGL DPLLFLAWEN VQRHQDMISV DLKTPLVIFK CHHGFHQTCL ENLAQKPDEY1260
 1261 SCLICQTESN PKIV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		PEP3 PEP5 VPS16 VPS33 VPS41 VPS8
View Details	Gavin AC, et al. (2006)		PEP3 PEP5 VAM6 VPS16 VPS33 VPS41 VPS8
View Details	Gavin AC, et al. (2002)		ATP8 LUC7 PEP3 PEP5 SNU71 VAM6 VPS16 VPS33 VPS41 VPS8
View Details	Ho Y, et al. (2002)		BGL2 CBP6 FRS2 GCD11 GCN3 HXT7 MCX1 NDE1 NPA3 PET10 PET9 SLC1 TRP3 TRP5 VPS8
View Details	Qiu et al. (2008)		PEP3 VPS8

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD51	VPS8	1	Unpublished Fields Lab Data
View Screen	HXK2	VPS8	1	Unpublished Fields Lab Data
View Screen	AML1	VPS8	1	Unpublished Fields Lab Data
View Screen	SPO1	VPS8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	VPS8	Huh WK, et al. (2003)
View Data	VPS8 and SNF7	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..450]	PSI-BLAST	40.69897	Actin interacting protein 1
2	View Details	[451..638]	deduced	N/A	No confident structure predictions are available.
3	View Details	[639..772]	deduced	N/A	No confident structure predictions are available.
4	View Details	[773..1091]	PSI-BLAST	13.30103	Clathrin heavy chain proximal leg segment
5	View Details	[1092..1190]	deduced	N/A	No confident structure predictions are available.
6	View Details	[1191..1274]	MSA	3.657997	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	1.8484919978091	bayes_pls_golite062009
protein binding	1.42618832381942	bayes_pls_golite062009
transporter activity	1.21549336905926	bayes_pls_golite062009
substrate-specific transporter activity	0.734793983890004	bayes_pls_golite062009
hydrolase activity	0.36638536879917	bayes_pls_golite062009
cytoskeletal protein binding	0.359536347486199	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.00585398682921312	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
binding	2.13191655110108	bayes_pls_golite062009
protein binding	1.69332800381364	bayes_pls_golite062009
small conjugating protein ligase activity	1.19185232032795	bayes_pls_golite062009
ubiquitin-protein ligase activity	1.18697373227452	bayes_pls_golite062009
hydrolase activity	0.737434311410924	bayes_pls_golite062009
catalytic activity	0.168711389690753	bayes_pls_golite062009
peptidase activity	0.0046487386791938	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.62

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2