Protein Overview: Lis-1-PB, Lis-1-...

General Information:
Name(s) found:	FBpp0293289 [FlyBase] Lis-1-PF / FBpp0089100 [FlyBase] Lis-1-PB / FBpp0086379 [FlyBase] Lis-1-PA / FBpp0086375 [FlyBase]
Description(s) found: Found 83 descriptions. SHOW ALL	type=protein; loc=2R:join(12069558..12069595,12069663..12069963,12070022..12070178,12070242..12070419,12070488..12070623,12070691..12071042,12071108..12071181); ID=FBpp0293289; name=Lis-1-PG; parent=FBgn0015754,FBtr0304747; dbxref=REFSEQ:NP_001246361,GB_protein:AFH08114,FlyBase:FBpp0293289,FlyBase_Annotation_IDs:CG8440-PG; MD5=0df75cd34451ff7946319bc58a2e76d0; length=411; release=r5.56; species=Dmel; [FlyBase] The gene Lissencephaly-1 is referred to in FlyBase by the symbol Dmel\Lis-1 (CG8440, FBgn0015754). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is 2R:12066309..12072612. It has the cytological map location 52F4-52F5. Based on sequence similarity, it is predicted to have molecular function</span>: ATPase activity, coupled; dynein binding; enzyme regulator activity. There is experimental evidence for 23 unique biological process</span> terms, many of which group under: reproductive process in a multicellular organism; anatomical structure development; transport; organelle organization; cell cycle; cellular localization; cellular macromolecule localization; microtubule-based movement; oocyte fate determination; cell cycle process. 27 alleles are reported. The phenotypes of these alleles are annotated with: nurse cell fusome; oocyte; microtubule organizing center; spindle; egg chamber; oocyte nucleus; microtubule; spindle pole centrosome; dorsal appendage; germ band. It has 6 annotated transcripts and 6 annotated polypeptides. [FlyBase] The gene Lissencephaly-1 is referred to in FlyBase by the symbol Dmel\Lis-1 (CG8440, FBgn0015754). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is 2R:12066309..12072612. It has the cytological map location 52F4-52F5. Based on sequence similarity, it is predicted to have molecular function</span>: ATPase activity, coupled; dynein binding; enzyme regulator activity. There is experimental evidence for 23 unique biological process</span> terms, many of which group under: reproductive process in a multicellular organism; anatomical structure development; transport; organelle organization; cell cycle; cellular localization; cellular macromolecule localization; microtubule-based movement; oocyte fate determination; cell cycle process. 27 alleles are reported. The phenotypes of these alleles are annotated with: nurse cell fusome; oocyte; microtubule organizing center; spindle; egg chamber; oocyte nucleus; microtubule; spindle pole centrosome; dorsal appendage; germ band. It has 6 annotated transcripts and 6 annotated polypeptides. [FlyBase] The gene Lissencephaly-1 is referred to in FlyBase by the symbol Dmel\Lis-1 (CG8440, FBgn0015754). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is 2R:12066309..12072612. It has the cytological map location 52F4-52F5. Based on sequence similarity, it is predicted to have molecular function</span>: ATPase activity, coupled; dynein binding; enzyme regulator activity. There is experimental evidence for 23 unique biological process</span> terms, many of which group under: reproductive process in a multicellular organism; anatomical structure development; transport; organelle organization; cell cycle; cellular localization; cellular macromolecule localization; microtubule-based movement; oocyte fate determination; cell cycle process. 27 alleles are reported. The phenotypes of these alleles are annotated with: nurse cell fusome; oocyte; microtubule organizing center; spindle; egg chamber; oocyte nucleus; microtubule; spindle pole centrosome; dorsal appendage; germ band. It has 6 annotated transcripts and 6 annotated polypeptides. [FlyBase]
Organism:	Drosophila melanogaster
Length:	411 amino acids
Gene Ontology:
Cellular Component:	dendrite [IDA] spindle pole centrosome [IDA] dynactin complex [IDA] cell cortex [IDA] kinetochore microtubule [IDA] dynein complex [IDA][ISS] neuronal cell body [IDA] kinetochore [IDA] axon [IDA]
Biological Process:	mitotic cell cycle checkpoint [IGI][IMP] axon cargo transport [IMP] germarium-derived oocyte fate determination [IMP] protein localization [IGI][IMP] oocyte nucleus migration during oocyte axis specification [IMP] microtubule-based movement [ISS] intracellular protein transport [IMP] regulation of dendrite morphogenesis [IMP] dendrite morphogenesis [IMP] spindle assembly [IMP] neuroblast proliferation [IMP] kinetochore organization [IMP] germarium-derived oocyte differentiation [IMP] regulation of catalytic activity [ISS] nucleus localization [IMP] intracellular mRNA localization [IMP] chorion-containing eggshell pattern formation [IMP] mushroom body development [IMP] fusome organization [IMP] cytoplasmic transport, nurse cell to oocyte [IMP] centrosome separation [IMP] oocyte fate determination [IGI] oogenesis [IMP] female germ-line cyst formation [IMP] nuclear migration along microtubule [IGI]
Molecular Function:	enzyme regulator activity [ISS] dynein binding [ISS] ATPase activity, coupled [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKMVLSQRQR EELNQAIADY LGSNGYADSL ETFRKEADLS TEVEKKFGGL LEKKWTSVIR  60
   61 LQKKVMELEA KLTEAEKEVI EGAPTKNKRT PGEWIPRPPE KFSLTGHRAS ITRVIFHPIF 120
  121 ALMVSASEDA TIRIWDFETG EYERSLKGHT DSVQDVAFDA QGKLLASCSA DLSIKLWDFQ 180
  181 QSYECIKTMH GHDHNVSSVA FVPAGDYVLS ASRDRTIKMW EVATGYCVKT YTGHREWVRM 240
  241 VRVHIEGSIF ATCSNDQTIR VWLTNSKDCK VELRDHEHTV ECIAWAPEAA ASAINEAAGA 300
  301 DNKKGHHQGP FLASGSRDKT IRIWDVSVGL CLLTLSGHDN WVRGLAFHPG GKYLVSASDD 360
  361 KTIRVWDLRN KRCMKTLYAH QHFCTSIDFH KAHPYVISGS VDQTVKVWEC R

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
dynactin binding	7.11157019623125	bayes_pls_golite062009
	6.67009948605948	bayes_pls_golite062009
dynein heavy chain binding	6.59987903164261	bayes_pls_golite062009
snoRNA binding	5.21229097335349	bayes_pls_golite062009
structural molecule activity	5.05435272846365	bayes_pls_golite062009
histone methyltransferase activity (H3-K4 specific)	4.71992275913384	bayes_pls_golite062009
GTPase activity	4.02994814377904	bayes_pls_golite062009
actin binding	3.86142918698875	bayes_pls_golite062009
acetyltransferase activity	3.85374902981345	bayes_pls_golite062009
RNA binding	3.77273677738815	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	3.31760161664239	bayes_pls_golite062009
S-adenosylmethionine-dependent methyltransferase activity	3.25521258308263	bayes_pls_golite062009
cytoskeletal protein binding	3.1056443224755	bayes_pls_golite062009
tubulin binding	2.76945430061825	bayes_pls_golite062009
microtubule binding	2.72684103222924	bayes_pls_golite062009
dynein binding	2.54821997913979	bayes_pls_golite062009
peroxisome targeting sequence binding	2.43196533618619	bayes_pls_golite062009
binding	2.42570478946173	bayes_pls_golite062009
protein binding	2.26925404328312	bayes_pls_golite062009
unfolded protein binding	2.07597866771866	bayes_pls_golite062009
actin filament binding	2.0735069176589	bayes_pls_golite062009
transcription repressor activity	2.03485691549401	bayes_pls_golite062009
transcription regulator activity	1.95667720244696	bayes_pls_golite062009
nucleic acid binding	1.90457914882449	bayes_pls_golite062009
histone methyltransferase activity	1.85632145559025	bayes_pls_golite062009
G-protein-coupled receptor binding	1.63631972867316	bayes_pls_golite062009
acyltransferase activity	1.6301799051509	bayes_pls_golite062009
transferase activity, transferring acyl groups other than amino-acyl groups	1.59523251984393	bayes_pls_golite062009
transferase activity, transferring acyl groups	1.46155402212921	bayes_pls_golite062009
DNA binding	1.38475369554616	bayes_pls_golite062009
platelet-activating factor acetyltransferase activity	1.36229564654648	bayes_pls_golite062009
protein methyltransferase activity	1.35170368882651	bayes_pls_golite062009
peroxisome matrix targeting signal-2 binding	1.26984584070766	bayes_pls_golite062009
transporter activity	1.21549336905926	bayes_pls_golite062009
transcription corepressor activity	1.2107661925348	bayes_pls_golite062009
histone-lysine N-methyltransferase activity	1.15313682561559	bayes_pls_golite062009
protein-lysine N-methyltransferase activity	1.15313682561559	bayes_pls_golite062009
lysine N-methyltransferase activity	1.15313682561559	bayes_pls_golite062009
receptor activity	1.12819778129653	bayes_pls_golite062009
mRNA binding	1.05053985350578	bayes_pls_golite062009
microtubule plus-end binding	1.01166033978052	bayes_pls_golite062009
myosin binding	0.989691178611801	bayes_pls_golite062009
molecular transducer activity	0.974380908248463	bayes_pls_golite062009
signal transducer activity	0.974380908248463	bayes_pls_golite062009
protein transporter activity	0.964340340346761	bayes_pls_golite062009
type 1 angiotensin receptor binding	0.920323565472959	bayes_pls_golite062009
angiotensin receptor binding	0.920323565472959	bayes_pls_golite062009
myosin heavy chain kinase activity	0.848140419140051	bayes_pls_golite062009
kinase activity	0.807669232329985	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.805273887494167	bayes_pls_golite062009
transcription factor activity	0.804334981068351	bayes_pls_golite062009
substrate-specific transporter activity	0.734793983890004	bayes_pls_golite062009
dynein intermediate chain binding	0.707581908546932	bayes_pls_golite062009
histone binding	0.685319917508843	bayes_pls_golite062009
transcription initiation factor activity	0.656824577987243	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.634547834947756	bayes_pls_golite062009
protein kinase activity	0.544118712921054	bayes_pls_golite062009
insulin receptor binding	0.43316396073854	bayes_pls_golite062009
transcription factor binding	0.412844855996155	bayes_pls_golite062009
nucleotidyltransferase activity	0.28606552120995	bayes_pls_golite062009
transcription cofactor activity	0.19391619547146	bayes_pls_golite062009
chromatin binding	0.190739938818992	bayes_pls_golite062009
telomerase activity	0.18450996813475	bayes_pls_golite062009
enzyme regulator activity	0.161639372576406	bayes_pls_golite062009
myosin II binding	0.12060669039328	bayes_pls_golite062009
structural constituent of cytoskeleton	0.118975415284669	bayes_pls_golite062009
protein serine/threonine kinase activity	0.0803897416817422	bayes_pls_golite062009
receptor binding	0.0390635451962911	bayes_pls_golite062009
kinase binding	0.0366162235750993	bayes_pls_golite062009
protein kinase binding	0.00858004043229577	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.00585398682921312	bayes_pls_golite062009
N-methyltransferase activity	0.00335766408304172	bayes_pls_golite062009

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.94

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..411]	PSI-BLAST	93.69897	PAF-AH Holoenzyme: Lis1/Alfa2

Protein Overview: Lis-1-PB, Lis-1-...

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: