Protein Overview: MED1

General Information:
Name(s) found:	MED1 / YPR070W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|805050\|emb\|CAA89187.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|74676582\|sp\|Q12321.1\|MED1_YEAST RecName: Full=Mediator of RNA polymerase II transcription subunit 1; AltName: Full=Mediator complex subunit 1 [NCBI NR] gi\|6325327\|ref\|NP_015395.1\| Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; Med1p [Saccharomyces cerevisiae] [NCBI NR] gi\|1314138\|emb\|CAA94978.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|1230678\|gb\|AAB68121.1\| Ypr070wp [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S54091 hypothetical protein YPR070w - yeast (Saccharomyces cerevisiae) [NCBI NR] Mediator of RNA polymerase II transcription subunit 1 OS=Saccharomyces cerevisiae GN=MED1 PE=1 SV=1 [Swiss-Prot] MED1 Component of RNA polymerase II holoenzyme and mediator subcomplex [mips] Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation [SGD] >Q12321 (Q12321) MED1 protein [4932.FASTACCERV.txt] Chr XVI from 685893-687593 [hmr_yeast5_DM3-1_pombe.fasta] MED1, Chr XVI from 685893-687593 [yeast_orfs2] MED1 SGDID:S0006274, Chr XVI from 685893-687593, Verified ORF [yeastflysequest.fasta] MED1 SGDID:S0006274, Chr XVI from 685895-687595, Verified ORF [10112005cervSGD.fasta] Ypr070wp [Saccharomyces cerevisiae]gi\|6325327\|ref\|NP_015395.1\| Med1p [Saccharomyces cerevisiae]gi\|805050\|emb\|CAA89187.1\| unknown [Saccharomyces cerevisiae]gi\|1314138\|emb\|CAA94978.1\| unknown [Saccharomyces cerevisiae]gi\|1084792\|pir\|\|S54091 hypothetical [nrdb_11152004_Chlamydomonas] (Q12321) Hypothetical protein YPR063W (Ypr070wp) [cerv4932.FASTAC] MED1 SGDID:S000006274, Chr XVI from 685895-687595, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; Med1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ypr070wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RNA polymerase II mediator complex subunit 1 [nr-271106-contam.fasta] [chemostatdb.fasta] Mediator of RNA polymerase II transcription subunit 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MED1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MED1 SGDID:S000006274, Chr XVI from 685898-687598, Genome Release 64-1-1, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation" [yeast-030211-contam.fasta] MED1 SGDID:S000006274, Chr XVI from 685898-687598, Genome Release 64-2-1, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation" [20141208_orf_trans.fasta] TR2698\|c0_g1_i1\|g.1898 ORF TR2698\|c0_g1_i1\|g.1898 TR2698\|c0_g1_i1\|m.1898 type:complete len:567 (+) TR2698\|c0_g1_i1:264-1964(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YPR070W SGDID:S000006274, Chr XVI from 685898-687598, Genome Release 64-1-1, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_3662\|g.2308 ORF asmbl_3662\|g.2308 asmbl_3662\|m.2308 type:complete len:567 (+) asmbl_3662:264-1964(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_3265\|g.2204 ORF ID=asmbl_3265\|g.2204 ID=asmbl_3265\|m.2204 type:complete len:567 (+) ID=asmbl_3265:264-1964(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	566 amino acids
Gene Ontology:
Cellular Component:	nucleus [IEA] mediator complex [IDA][IEA]
Biological Process:	telomere maintenance [IMP] transcription from RNA polymerase II promoter [IDA]
Molecular Function:	RNA polymerase II transcription mediator activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVEGDSYVET LDSMIELFKD YKPGSITLEN ITRLCQTLGL ESFTEELSNE LSRLSTASKI  60
   61 IVIDVDYNKK QDRIQDVKLV LASNFDNFDY FNQRDGEHEK SNILLNSLTK YPDLKAFHNN 120
  121 LKFLYLLDAY SHIESDSTSH NNGSSDKSLD SSNASFNNQG KLDLFKYFTE LSHYIRQCFQ 180
  181 DNCCDFKVRT NLNDKFGIYI LTQGINGKEV PLAKIYLEEN KSDSQYRFYE YIYSQETKSW 240
  241 INESAENFSN GISLVMEIVA NAKESNYTDL IWFPEDFISP ELIIDKVTCS SNSSSSPPII 300
  301 DLFSNNNYNS RIQLMNDFTT KLINIKKFDI SNDNLDLISE ILKWVQWSRI VLQNVFKLVS 360
  361 TPSSNSNSSE LEPDYQAPFS TSTKDKNSST SNTEPIPRSN RHGSVVEASR RRRSSTNKSK 420
  421 RPSITEAMML KEEGLQQFNL HEILSEPAIE EENGDSIKEH STTMDGANDL GFTASVSNQE 480
  481 NAGTDIVMED HGVLQGTSQN YGTATADDAD IEMKDVSSKP SKPESSVLQL IVSEDHIILD 540
  541 TISECNLYDD VKCWSKFIEK FQDIVS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CSE2 GAL11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SOH1 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SSN2 SSN3 YAP1
View Details	Krogan NJ, et al. (2006)		CSE2 DSS1 GAL11 GPT2 IRC11 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 ROX3 SIN4 SOH1 SRB2 SRB4 SRB5 SRB6 SRB7 SSN3 SSN8 THI72
View Details	Gavin AC, et al. (2006)		CSE2 GAL11 MED1 MED11 MED6 MED7 MED8 NUT1 NUT2 PGD1 RGR1 SIN4 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SSN2
View Details	Gavin AC, et al. (2002)		ADA2 ADH1 COG3 CSE2 ENO2 FAB1 FBA1 GAL11 GCN5 MED1 MED11 MED2 MED4 MED6 MED7 MED8 MLP1 MRPL6 NUT1 NUT2 PGD1 PGK1 RGR1 ROX3 RPG1 RPO21 SIN4 SPT7 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SRP1 SSE2 SSN2 SSN3 SSN8 TAF6 TIF1, TIF2 YIL077C
View Details	Qiu et al. (2008)		ADA2 CSE2 DMC1 GAL11 HFI1 MED1 MED11 MED2 MED4 MED6 MED7 MED8 NGG1 NUT1 NUT2 PGD1 RGR1 ROX3 SGF73 SIN4 SOH1 SPT7 SRB2 SRB4 SRB5 SRB6 SRB7 TAF10 TAF12 TAF14 TAF5 TAF9 TFB5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	MED1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..566]	deduced	N/A	No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: MED1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: