Protein Overview: SRP68

General Information:
Name(s) found:	SRP68 / YPL243W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|730824\|sp\|P38687.1\|SRP68_YEAST RecName: Full=Signal recognition particle subunit SRP68; AltName: Full=Signal recognition particle 68 kDa protein homolog [NCBI NR] gi\|6325013\|ref\|NP_015081.1\| Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; Srp68p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S47928 signal recognition particle protein SRP68 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|531016\|gb\|AAA53401.1\| signal recognition particle protein [NCBI NR] gi\|1370501\|emb\|CAA97964.1\| SRP68 [Saccharomyces cerevisiae] [NCBI NR] gi\|1061246\|emb\|CAA91601.1\| SRP68 [Saccharomyces cerevisiae] [NCBI NR] Signal recognition particle subunit SRP68 OS=Saccharomyces cerevisiae GN=SRP68 PE=1 SV=1 [Swiss-Prot] (P38687) Signal recognition particle 68 kDa protein homolog (SRP68) [Swiss-Prot] SRP68 Signal recognition particle protein [mips] Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane [SGD] Chr XVI from 88517-90316 [hmr_yeast5_DM3-1_pombe.fasta] SRP68, Chr XVI from 88517-90316 [yeast_orfs2] SRP68 SGDID:S0006164, Chr XVI from 88517-90316, Verified ORF [yeastflysequest.fasta] Component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in protein targeting to the endoplasmic reticulum (ER) membrane [Saccharomyces cerevisiae]gi\|1370501\|emb\|CAA97964.1\| SRP68 [Saccharomyces cerevisiae]gi\|106 [nrdb_11152004_Chlamydomonas] SRP68 SGDID:S000006164, Chr XVI from 88517-90316, Verified ORF, "Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Signal recognition particle subunit SRP68 (Signal recognition particle 68 kDa protein homolog) [nr-271106-contam.fasta] SRP68 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] signal recognition particle protein [nr-271106-contam.fasta] Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; Srp68p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Signal recognition particle subunit SRP68 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SRP68 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SRP68 SGDID:S000006164, Chr XVI from 88517-90316, Genome Release 64-1-1, Verified ORF, "Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane" [yeast-030211-contam.fasta] SRP68 SGDID:S000006164, Chr XVI from 88517-90316, Genome Release 64-2-1, Verified ORF, "Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress" [20141208_orf_trans.fasta] YPL243W SGDID:S000006164, Chr XVI from 88517-90316, Genome Release 64-1-1, Verified ORF, "Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	599 amino acids
Gene Ontology:
Cellular Component:	signal recognition particle, endoplasmic reticulum targeting [IDA]
Biological Process:	SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition [IC] protein targeting to ER [IPI]
Molecular Function:	signal sequence binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVAYSPIIAT YGNRAEQFLE TDSDFAKYHA KLNKKLQHLR SRCHLVTKDT KKYSSKNKYG  60
   61 EINSEDYDNK TKLIGVLILL HAERDLALAE TLKLRARQRG KLKKSEEKVL STRLKKACKT 120
  121 ADKLVNVTQN EQQWITRAQY LAFAKLVHSE YLINGKRFKR KDNAKISNNL ALVFAALEHL 180
  181 KNLSLLAEEV VDNIVNKYQY SLKQYAGNLI TTPEINNFIV ERVQSDENKD DELVKLLLDN 240
  241 GFNMKKITTS TEDQKVTTNI NWRSFNAKII DAEVAQFLEQ GLSIHPTQIT QYTQRLSKLE 300
  301 KALDRHEFFI ANHDDQDDID EMVENSSENN QIILAYIKYN ILLTSISRER DLFTHLWNQW 360
  361 LKLNTSLPSK LTKYKEMERI VKNLTKYLSD IMELPGVYSD DELLSQLDLC KLYFQLFLNT 420
  421 GCLSVLYQSK GRYMEALALY VDAYRRLENK LSEIESLDEI LLPANLLSLN SVRSLQKRIE 480
  481 NGGNSVITLA EYEKRNHGGS LGKYDLTVIE KLDSKKILPT DIQLKNLFPL KPKMLPIPSK 540
  541 PTLFDLAFNY ITYDKQEPSA SQVKDSVTET ESISQTPISN EQTEGEPKKK RGFLGLFGR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		LHP1 SEC65 SRP14 SRP21 SRP54 SRP68 SRP72
View Details	(MIPS) Mewes HW, et al. (2004)		SEC65 SRP14 SRP21 SRP54 SRP68 SRP72
View Details	Krogan NJ, et al. (2006)		LHP1 SEC65 SRP14 SRP21 SRP54 SRP68 SRP72 YCR090C
View Details	Gavin AC, et al. (2006)		SEC65 SRP14 SRP54 SRP68 SRP72
View Details	Gavin AC, et al. (2002)		BEM2 LHP1 SEC65 SRO9 SRP14 SRP21 SRP54 SRP68 SRP72
View Details	Qiu et al. (2008)		SEC65 SRP14 SRP21 SRP54 SRP68 SRP72

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YJR056C	SRP68	1	Unpublished Fields Lab Data
View Screen	UBC7	SRP68	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SRP68	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..96]	MSA	1.009985	View MSA. No confident structure predictions are available.
2	View Details	[97..227]	deduced	N/A	Confident ab initio structure predictions are available.
3	View Details	[228..599]	MSA	2.009901	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2