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Protein Overview: DUF1

General Information:
Name(s) found: DUF1 / YOL087C [SGD]
Description(s) found:
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  • gi|74698026|sp|Q99247.1|YO087_YEAST RecName: Full=Uncharacterized WD repeat-containing protein YOL087C [NCBI NR]
  • gi|6324485|ref|NP_014554.1| Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic [NCBI NR]
  • gi|600471|emb|CAA58192.1| orf 00944 [Saccharomyces cerevisiae] [NCBI NR]
  • gi|1419928|emb|CAA99099.1| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S57382 hypothetical protein YOL087c - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • Uncharacterized WD repeat-containing protein YOL087C OS=Saccharomyces cerevisiae GN=YOL087C PE=1 SV=1 [Swiss-Prot]
  • Protein of unknown function localised to cytoplasm [mips]
  • Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid [SGD]
  • >Q99247 (Q99247) ORF YOL087C from chromosome XV [4932.FASTACCERV.txt]
  • Chr XV from 158636-155286, reverse complement [hmr_yeast5_DM3-1_pombe.fasta]
  • Chr XV from 155286-158636, reverse complement [yeastorf3.fasta]
  • YOL087C, Chr XV from 155286-158636, reverse complement [yeast_orfs2]
  • YOL087C SGDID:S0005447, Chr XV from 158636-155286, reverse complement, Uncharacterized ORF [yeastflysequest.fasta]
  • Yol087cp [Saccharomyces cerevisiae]gi|600471|emb|CAA58192.1| orf 00944 [Saccharomyces cerevisiae]gi|1419928|emb|CAA99099.1| unnamed protein product [Saccharomyces cerevisiae]gi|1362357|pir||S57382 hypothetical protein YOL087c - yeast (Saccharomyces cer [nrdb_11152004_Chlamydomonas]
  • (Q99247) Orf 00944 protein (S.cerevisiae chromosome XV reading frame ORF YOL087c) [cerv4932.FASTAC]
  • YOL087C SGDID:S000005447, Chr XV from 158636-155286, reverse complement, Uncharacterized ORF, "Hypothetical protein" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • YOL087C SGDID:S000005447, Chr XV from 158637-155287, reverse complement, Uncharacterized ORF, "Hypothetical protein" [yeast-contam.fasta]
  • YOL087C SGDID:S000005447, Chr XV from 158637-155287, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; deletion mutant is sensitive to various chemica [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta]
  • orf 00944 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • hypothetical protein; Yol087cp [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • WD repeat protein YOL087C [nr-271106-contam.fasta]
  • unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • YOL087C SGDID:S000005447, Chr XV from 158637-155287, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • Uncharacterized WD repeat-containing protein YOL087C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YOL087C PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • YOL087C SGDID:S000005447, Chr XV from 158637-155287, Genome Release 64-1-1, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid" [yeast-030211-contam.fasta]
  • DUF1 SGDID:S000005447, Chr XV from 158637-155287, Genome Release 64-2-1, reverse complement, Verified ORF, "Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid" [20141208_orf_trans.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 1116 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed article]
    Biological Process: biological_process [ND]
    Molecular Function: molecular_function [ND]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNQLTVSYGL ISPDYCTSQD AHILPITKIL YPDIPGKNYF LTSGRDGSII LHKNTQLSNE  60
   61 PETAATTIKN DAIRMQVHSD WASDLIHVNM KNSDPSAGDT FISVSHDFSI VLISVNAQLT 120
  121 TWDKKIIGDH DDYIKCIVPI HYEMSNDYEL EEQEGGPDNV HDGINNGIVV DEQNNFLFVT 180
  181 GGLDRKIKLW CLSSGPEKMA TLLHTFDNAQ SNDTGSIYSM SPIIPKYSFD DNQTSRPFDF 240
  241 VAGDCNGDLI FYSCKYRKEV IRIQNAHRTN IKVVRTLDDS TRLISTSSDG VINVWDLNCR 300
  301 HDQTTGALQL PKKIGSWSWD SSIWCVQGTS LDKLYFGDSQ GNVMRANLSS YEDAKLTRIF 360
  361 KPDHHHHHHH HHEHEEQNIS TTDAKVKKYG GILDIALLPN EKLLFSFCTD SNLNVLDLTN 420
  421 NHFSVNEGGF ALTRSSLLTN RRHVITENTK GQMQRWDIVS CELLNTFDSS EGSFDDIVMK 480
  481 YTSKEILSHW CTVSVKVGML FVKINPKFLK TEVYGSALKD YQVVNNIEIN SDERYNLGKI 540
  541 VINSLFNEFI SYEVQKDKLL RKKIFSLKKK DLTNSLTLDT GYNSESKKNN KDKKRKSTFK 600
  601 ISSTLSIGNT NSSGTPPNSA PATPVMAETI VLEEQPLLQS ASDKAIDDSL ELVQPLPASK 660
  661 KPYFRTQSSG SLLSRKFKSF RSTSGRATTG LNTPEEPKGI LPDTPHVIND DSAFPQAINT 720
  721 TQQSKDATPE SMLWNHPFKL EQKLSAISSQ DLPSNNTHNK LRSSENSRAN STSTLEGNEK 780
  781 KKPEFMPDLL EQIQESYKQQ YMNTSSLKYL TKRLPVTKII KASSCPIIRV KSATLVLVHL 840
  841 WKEGSCGGRV LFSTLLPPSH VDNETVSGGK ENSKPPDDEE VDLQAVDDDK LGKYDLIDGE 900
  901 LGSRLNRRQI FEQLEENLPY WFAKALFRDI KTVEEQPKLN FLIMPWSSVG GSEAAGNENK 960
  961 KKFISASDTT ESSGNDSSDS SLGNGNEAVS PSTQQQFHNM LKFGRPKTSE QELNPTDLPR1020
 1021 ISEANVKLVA PGMIRVKKIK LYVADRFETK TPEMKAKMEP SLWLDLLCRG QVLDNDMTLN1080
 1081 TVRTLYWKSQ GDIVLEYRRK VHNSPLVHEV NGNEGK

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) DUF1 UBP13
    View Details Krogan NJ, et al. (2006) DUF1 UBP13 UBP9
    View Details Ho Y, et al. (2002) ATP3 BEM2 COP1 DUF1 EDE1 FOL2 HTB2 LHS1 NIP1 POR1 RPG1 SES1 SRV2 TIF34 TIF35 UBI4

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data DUF1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..338] PSI-BLAST 73.0 beta1-subunit of the signal-transducing G protein heterotrimer
    2 View Details [339..469] PSI-BLAST 62.69897 Tup1, C-terminal domain
    3 View Details [470..837] ab initio 11.54 No description for 1i3qA was found.
    4 View Details [838..948] ab initio 10.72 No description for 1m2vB was found.
    5 View Details [949..1116] MSA 2.004955 View MSA. No confident structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.96

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle