Protein Overview: CLN1

General Information:
Name(s) found:	CLN1 / YMR199W [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) [SGD]
Organism:	Saccharomyces cerevisiae
Length:	546 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	regulation of cyclin-dependent protein kinase activity [TAS]
Molecular Function:	cyclin-dependent protein kinase regulator activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNHSEVKTGL IVTAKQTYYP IELSNAELLT HYETIQEYHE EISQNVLVQS SKTKPDIKLI  60
   61 DQQPEMNPHQ TREAIVTFLY QLSVMTRVSN GIFFHAVRFY DRYCSKRVVL KDQAKLVVGT 120
  121 CLWLAAKTWG GCNHIINNVS IPTGGRFYGP NPRARIPRLS ELVHYCGGSD LFDESMFIQM 180
  181 ERHILDTLNW DVYEPMINDY ILNVDENCLI QYELYKNQLQ NNNSNGKEWS CKRKSQSSDD 240
  241 SDATVEEHIS SSPQSTGLDG DTTTMDEDEE LNSKIKLINL KRFLIDLSCW QYNLLKFELY 300
  301 EICNGMFSII NKFTNQDQGP FLSMPIGNDI NSNTQTQVFS IIINGIVNSP PSLVEVYKEQ 360
  361 YGIVPFILQV KDYNLELQKK LQLASTIDLT RKIAVNSRYF DQNASSSSVS SPSTYSSGTN 420
  421 YTPMRNFSAQ SDNSVFSTTN IDHSSPITPH MYTFNQFKNE SACDSAISVS SLPNQTQNGN 480
  481 MPLSSNYQNM MLEERNKENR IPNSSSAEIP QRAKFMTTGI FQNTGELTNR ASSISLSLRN 540
  541 HNSSQL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CDC28 CKS1 CLN1 CLN2
View Details	(MIPS) Mewes HW, et al. (2004)		CDC28 CLB1 CLB2 CLB3 CLB4 CLB5 CLB6 CLN1 CLN2 CLN3
View Details	Gavin AC, et al. (2002)		CDC28 CKS1 CLB3 CLN1 CLN2 DAL7 PCA1 SIC1 SRL3 YPL014W
View Details	Ho Y, et al. (2002)		BUR2 CDC28 CKS1 CLB2 CLB3 CLB5 CLN1 HYP2 PGM2 YDR170W-A YER138C
View Details	Qiu et al. (2008)		CDC28 CLB1 CLB2 CLB3 CLB4 CLB5 CLB6 CLN1 CLN2 CLN3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC28	CLN1	2	Unpublished Fields Lab Data
View Screen	CDC28	CLN1	2	Unpublished Fields Lab Data
View Screen	CAB3	CLN1	1	Unpublished Fields Lab Data
View Screen	PRP16	CLN1	1	Unpublished Fields Lab Data
View Screen	CTF13	CLN1	1	Unpublished Fields Lab Data
View Screen	SEC17	CLN1	1	Unpublished Fields Lab Data
View Screen	THG1	CLN1	1	Hazbun TR, et al. (2003)
View Screen	MET18	CLN1	1	Unpublished Fields Lab Data
View Screen	CAB3	CLN1	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CLN1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..48] [197..386]	PSI-BLAST	133.09691	Cyclin A
2	View Details	[49..196]	PSI-BLAST	133.09691	Cyclin A
3	View Details	[387..546]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
cyclin-dependent protein kinase regulator activity	6.12919068656167	bayes_pls_golite062009
cyclin-dependent protein kinase activity	3.81462270101479	bayes_pls_golite062009
protein kinase regulator activity	2.90083336559452	bayes_pls_golite062009
kinase regulator activity	2.84789294954357	bayes_pls_golite062009
binding	2.4204867358489	bayes_pls_golite062009
transcription regulator activity	1.86024002251052	bayes_pls_golite062009
protein binding	1.47106731485576	bayes_pls_golite062009
nucleic acid binding	1.23878797237176	bayes_pls_golite062009
DNA binding	1.18961617410859	bayes_pls_golite062009
enzyme regulator activity	1.07025055509935	bayes_pls_golite062009
protein kinase activity	0.957590284872001	bayes_pls_golite062009
kinase activity	0.919118452831504	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.806564872314381	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.671643397144957	bayes_pls_golite062009
hydrolase activity	0.62079213929979	bayes_pls_golite062009
protein serine/threonine kinase activity	0.567809158275482	bayes_pls_golite062009
transcription factor activity	0.567104149291415	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.56301729141837	bayes_pls_golite062009
kinase binding	0.451409627821468	bayes_pls_golite062009
transcription factor binding	0.396734044657235	bayes_pls_golite062009
enzyme binding	0.145967754156613	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2