Protein Overview: NDI1

General Information:
Name(s) found:	NDI1 / YML120C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|805022\|emb\|CAA89160.1\| Ndi1p [Saccharomyces cerevisiae] [NCBI NR] gi\|6323515\|ref\|NP_013586.1\| NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; homolog of [NCBI NR] gi\|51830478\|gb\|AAU09768.1\| YML120C [Saccharomyces cerevisiae] [NCBI NR] gi\|417349\|sp\|P32340.1\|NDI1_YEAST RecName: Full=Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial; AltName: Full=Internal NADH dehydrogenase; Flags: Precursor [NCBI NR] pir\|\|S26704 NADH2 dehydrogenase (ubiquinone) (EC 1.6.5.3) chain NDI1 - yeast (Saccharomyces cerevisiae) [NCBI NR] Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial OS=Saccharomyces cerevisiae GN=NDI1 PE=1 SV=1 [Swiss-Prot] NDI1 NADH-ubiquinone-6 oxidoreductase [mips] NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; homolog of human AMID [SGD] NDI1, Chr XIII from 28266-29807, reverse complement [yeast_orfs2] Chr XIII from 28266-29807, reverse complement [yeastorf3.fasta] Chr XIII from 29807-28266, reverse complement [yeastorf5.fasta] (P32340) Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial precursor (EC 1.6.5.3) (Internal NADH dehydrogenase) [4932.FASTACCERV.txt] NDI1 SGDID:S0004589, Chr XIII from 29807-28266, reverse complement, Verified ORF [yeastflysequest.fasta] NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I which is absent in S. cerevisiae [Saccharomyces cerevis [nrdb_11152004_Chlamydomonas] NDI1 SGDID:S000004589, Chr XIII from 29807-28266, reverse complement, Verified ORF, "NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multi [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; homolog of human AMID; Ndi1p [Saccharomyces cerevisiae [nr-271106-contam.fasta] YML120C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ndi1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial precursor (Internal NADH dehydrogenase) [nr-271106-contam.fasta] NDI1 SGDID:S000004589, Chr XIII from 29807-28266, reverse complement, Verified ORF, "NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; homolog of human AMID" [yeast-bovrabprots.fasta] NDI1 SGDID:S000004589, Chr XIII from 29807-28266, reverse complement, Verified ORF, "NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; homolog of human AMID" [yeast-contam_custom.fasta] [chemostatdb.fasta] Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NDI1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NDI1 SGDID:S000004589, Chr XIII from 29807-28266, Genome Release 64-1-1, reverse complement, Verified ORF, "NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; homolog of human AMID" [yeast-030211-contam.fasta] NDI1 SGDID:S000004589, Chr XIII from 29807-28266, Genome Release 64-2-1, reverse complement, Verified ORF, "NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID" [20141208_orf_trans.fasta] YML120C SGDID:S000004589, Chr XIII from 29807-28266, Genome Release 64-1-1, reverse complement, Verified ORF, "NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; homolog of human AMID" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	513 amino acids
Gene Ontology:
Cellular Component:	mitochondrial matrix [IDA][IMP] mitochondrion [IDA]
Biological Process:	mitochondrial electron transport, NADH to ubiquinone [IDA] positive regulation of apoptosis [IMP] NADH oxidation [IDA] chronological cell aging [IMP]
Molecular Function:	NADH dehydrogenase (ubiquinone) activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLSKNLYSNK RLLTSTNTLV RFASTRSTGV ENSGAGPTSF KTMKVIDPQH SDKPNVLILG  60
   61 SGWGAISFLK HIDTKKYNVS IISPRSYFLF TPLLPSAPVG TVDEKSIIEP IVNFALKKKG 120
  121 NVTYYEAEAT SINPDRNTVT IKSLSAVSQL YQPENHLGLH QAEPAEIKYD YLISAVGAEP 180
  181 NTFGIPGVTD YGHFLKEIPN SLEIRRTFAA NLEKANLLPK GDPERRRLLS IVVVGGGPTG 240
  241 VEAAGELQDY VHQDLRKFLP ALAEEVQIHL VEALPIVLNM FEKKLSSYAQ SHLENTSIKV 300
  301 HLRTAVAKVE EKQLLAKTKH EDGKITEETI PYGTLIWATG NKARPVITDL FKKIPEQNSS 360
  361 KRGLAVNDFL QVKGSNNIFA IGDNAFAGLP PTAQVAHQEA EYLAKNFDKM AQIPNFQKNL 420
  421 SSRKDKIDLL FEENNFKPFK YNDLGALAYL GSERAIATIR SGKRTFYTGG GLMTFYLWRI 480
  481 LYLSMILSAR SRLKVFFDWI KLAFFKRDFF KGL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		AIM45 ALD4 CIR1 CIR2 CIT1 CYB2 DLD1 FUM1 GUT2 MDH1 NDE1 NDE2 NDI1 SDH1
View Details	Qiu et al. (2008)		AIF1 ALD4 ATP1 ATP2 COX5A COX5B COX7 CYB2 CYC7 CYT1 FUM1 GUT2 MDH1 NDE1 NDE2 NDI1 RPM2 SDH1 SDH4

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MIH1	experimental2 - mih1-2	McCusker D, et al (2007)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CNB1	NDI1	1	Unpublished Fields Lab Data
View Screen	ELM1	NDI1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NDI1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..179] [341..513]	PSI-BLAST	101.0	Dihydrolipoamide dehydrogenase
2	View Details	[180..340]	PSI-BLAST	101.0	Dihydrolipoamide dehydrogenase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
oxidoreductase activity, acting on NADH or NADPH	6.80483427531026	bayes_pls_golite062009
oxidoreductase activity	4.24087829378489	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	2.57282017498189	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	2.50603438449246	bayes_pls_golite062009
cation transmembrane transporter activity	2.3998060954585	bayes_pls_golite062009
ion transmembrane transporter activity	2.35426029070392	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	2.28982462439281	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.19095357354283	bayes_pls_golite062009
substrate-specific transporter activity	2.17217047087399	bayes_pls_golite062009
dihydrolipoyl dehydrogenase activity	1.6187224316494	bayes_pls_golite062009
oxygen-dependent protoporphyrinogen oxidase activity	1.59912481901166	bayes_pls_golite062009
catalytic activity	1.56773888754354	bayes_pls_golite062009
oxidoreductase activity, acting on sulfur group of donors, NAD or NADP as acceptor	1.45104542724719	bayes_pls_golite062009
oxidoreductase activity, acting on sulfur group of donors	1.31328088143083	bayes_pls_golite062009
protein binding	1.16738144711505	bayes_pls_golite062009
binding	0.993436593091194	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-CH group of donors	0.7227919966299	bayes_pls_golite062009
coenzyme binding	0.146916113354806	bayes_pls_golite062009
succinate dehydrogenase activity	0.110826254138262	bayes_pls_golite062009
cofactor binding	0.0578189575854124	bayes_pls_golite062009
NADPH-adrenodoxin reductase activity	0.0194702205549504	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2