Protein Overview: RAD5

General Information:
Name(s) found:	RAD5 / YLR032W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323060\|ref\|NP_013132.1\| DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; contains RING finger domain; Rad5p [Saccharomyces cerevisiae] [NCBI NR] gi\|417587\|sp\|P32849.1\|RAD5_YEAST RecName: Full=DNA repair protein RAD5; AltName: Full=Radiation sensitivity protein 5; AltName: Full=Revertibility protein 2 [NCBI NR] pir\|\|S64859 DNA repair protein RAD5 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|172347\|gb\|AAA34951.1\| RAD5 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1360348\|emb\|CAA97556.1\| RAD5 [Saccharomyces cerevisiae] [NCBI NR] DNA repair protein RAD5 OS=Saccharomyces cerevisiae GN=RAD5 PE=1 SV=1 [Swiss-Prot] RAD5 DNA helicase [mips] DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p [SGD] RAD5, Chr XII from 204992-208501 [yeast_orfs2] Chr XII from 204992-208501 [yeastorf3.fasta] (P32849) DNA repair protein RAD5 [4932.FASTACCERV.txt] RAD5 SGDID:S0004022, Chr XII from 204992-208501, Verified ORF [yeastflysequest.fasta] Rad5p [Saccharomyces cerevisiae]gi\|1360348\|emb\|CAA97556.1\| RAD5 [Saccharomyces cerevisiae]gi\|417587\|sp\|P32849\|RAD5_YEAST DNA repair protein RAD5gi\|2131233\|pir\|\|S64859 DNA repair protein RAD5 - yeast (Saccharomyces cerevisiae)gi\|172347\|gb\|AAA34951.1\| R [nrdb_11152004_Chlamydomonas] RAD5 SGDID:S000004022, Chr XII from 204992-208501, Verified ORF, "Single-stranded DNA-dependent ATPase, involved in postreplication repair; contains RING finger domain" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RAD5 SGDID:S000004022, Chr XII from 204992-208501, Verified ORF, "DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; contains RING finger domain" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] DNA repair protein RAD5 (Radiation sensitivity protein 5) (Revertibility protein 2) [nr-271106-contam.fasta] RAD5 protein [nr-271106-contam.fasta] RAD5 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Single-stranded DNA-dependent ATPase, involved in postreplication repair; contains RING finger domain; Rad5p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RAD5 SGDID:S000004022, Chr XII from 204992-208501, Verified ORF, "DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free a [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] [chemostatdb.fasta] RAD5 SGDID:S000004022, Chr XII from 204992-208501, Verified ORF, "DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p" [SGDyeast.20101221.fasta] DNA repair protein RAD5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RAD5 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RAD5 SGDID:S000004022, Chr XII from 204991-208500, Genome Release 64-1-1, Verified ORF, "DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p" [yeast-030211-contam.fasta] RAD5 SGDID:S000004022, Chr XII from 204991-208500, Genome Release 64-2-1, Verified ORF, "DNA helicase/Ubiquitin ligase; involved in error-free branch of DNA damage tolerance (DDT) pathway; proposed to promote replication fork regression during postreplication repair by template switching; stimulates synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress; associates with native telomeres, cooperates with homologous recombination in senescent cells" [20141208_orf_trans.fasta] YLR032W SGDID:S000004022, Chr XII from 204991-208500, Genome Release 64-1-1, Verified ORF, "DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1169 amino acids
Gene Ontology:
Cellular Component:	nuclear chromatin [IDA]
Biological Process:	DNA repair [IMP] telomere maintenance [IMP] double-strand break repair [IGI][IMP]
Molecular Function:	ATPase activity [TAS] single-stranded DNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSHIEQEERK RFFNDDLDTS ETSLNFKSEN KESFLFANSH NDDDDDVVVS VSDTTEGEGD  60
   61 RSIVPVRREI EEEGQNQFIT ELLRIIPEMP KDLVMELNEK FGSQEEGLSL ALSHYFDHNS 120
  121 GTSISKIPSS PNQLNTLSDT SNSTLSPSSF HPKRRRIYGF RNQTRLEDKV TWKRFIGALQ 180
  181 VTGMATRPTV RPLKYGSQMK LKRSSEEISA TKVYDSRGRK KASMASLVRI FDIQYDREIG 240
  241 RVSEDIAQIL YPLLSSHEIS FEVTLIFCDN KRLSIGDSFI LQLDCFLTSL IFEERNDGES 300
  301 LMKRRRTEGG NKREKDNGNF GRTLTETDEE LESRSKRLAL LKLFDKLRLK PILDEQKALE 360
  361 KHKIELNSDP EIIDLDNDEI CSNQVTEVHN NLRDTQHEEE TMNLNQLKTF YKAAQSSESL 420
  421 KSLPETEPSR DVFKLELRNY QKQGLTWMLR REQEFAKAAS DGEASETGAN MINPLWKQFK 480
  481 WPNDMSWAAQ NLQQDHVNVE DGIFFYANLH SGEFSLAKPI LKTMIKGGIL SDEMGLGKTV 540
  541 AAYSLVLSCP HDSDVVDKKL FDIENTAVSD NLPSTWQDNK KPYASKTTLI VVPMSLLTQW 600
  601 SNEFTKANNS PDMYHEVYYG GNVSSLKTLL TKTKTPPTVV LTTYGIVQNE WTKHSKGRMT 660
  661 DEDVNISSGL FSVNFYRIII DEGHNIRNRT TVTSKAVMAL QGKCKWVLTG TPIINRLDDL 720
  721 YSLVKFLELD PWRQINYWKT FVSTPFESKN YKQAFDVVNA ILEPVLLRRT KQMKDKDGKP 780
  781 LVELPPKEVV IKRLPFSKSQ DLLYKFLLDK AEVSVKSGIA RGDLLKKYST ILVHILRLRQ 840
  841 VCCHPGLIGS QDENDEDLSK NNKLVTEQTV ELDSLMRVVS ERFDNSFSKE ELDAMIQRLK 900
  901 VKYPDNKSFQ SLECSICTTE PMDLDKALFT ECGHSFCEKC LFEYIEFQNS KNLGLKCPNC 960
  961 RNQIDACRLL ALVQTNSNSK NLEFKPYSPA SKSSKITALL KELQLLQDSS AGEQVVIFSQ1020
 1021 FSTYLDILEK ELTHTFSKDV AKIYKFDGRL SLKERTSVLA DFAVKDYSRQ KILLLSLKAG1080
 1081 GVGLNLTCAS HAYMMDPWWS PSMEDQAIDR LHRIGQTNSV KVMRFIIQDS IEEKMLRIQE1140
 1141 KKRTIGEAMD TDEDERRKRR IEEIQMLFE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ALT2 RAD5 RRP1
View Details	Qiu et al. (2008)		PCC1 RAD18 RAD5 RAP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ASF1	3829 - low filter	Green EM, et al (2005)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HMT1	RAD5	1	Unpublished Fields Lab Data
View Screen	TAM41	RAD5	1	Hazbun TR, et al. (2003)
View Screen	UBC7	RAD5	1	Unpublished Fields Lab Data
View Screen	PRM3	RAD5	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RAD5	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..378]	MSA	5.209996	View MSA. No confident structure predictions are available.
2	View Details	[379..476]	ORFEUS	11.29	RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
3	View Details	[477..819]	ORFEUS	11.29	RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
4	View Details	[820..921]	MSA	1.345993	View MSA. No confident structure predictions are available.
5	View Details	[922..975]	deduced	N/A	Confident ab initio structure predictions are available.
6	View Details	[976..1169]	PSI-BLAST	2.522879	Nucleotide excision repair enzyme UvrB

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
DNA-dependent ATPase activity	2.72462789867829	bayes_pls_golite062009
DNA helicase activity	2.2817164193758	bayes_pls_golite062009
binding	2.05253348982328	bayes_pls_golite062009
ATP-dependent DNA helicase activity	1.88455339138556	bayes_pls_golite062009
nucleoside-triphosphatase activity	1.84050240798436	bayes_pls_golite062009
pyrophosphatase activity	1.81138802583782	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	1.78927528875848	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	1.78903889318615	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
microtubule motor activity	1.35938517416356	bayes_pls_golite062009
motor activity	1.32129340699286	bayes_pls_golite062009
protein-DNA loading ATPase activity	1.29640539057136	bayes_pls_golite062009
hydrolase activity	1.29419296935491	bayes_pls_golite062009
structure-specific DNA binding	1.2539059700735	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATPase activity	1.18431260772292	bayes_pls_golite062009
DNA-directed DNA polymerase activity	1.17617511072239	bayes_pls_golite062009
DNA polymerase activity	1.14104131900023	bayes_pls_golite062009
DNA clamp loader activity	1.12431517057397	bayes_pls_golite062009
double-stranded DNA binding	1.117585097627	bayes_pls_golite062009
nucleic acid binding	1.08697924060619	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
DNA binding	0.702147676354075	bayes_pls_golite062009
single-stranded DNA binding	0.568457189190858	bayes_pls_golite062009
mismatched DNA binding	0.31144582840038	bayes_pls_golite062009
transcription regulator activity	0.287777990975748	bayes_pls_golite062009
DNA strand annealing activity	0.283577486331079	bayes_pls_golite062009
nucleotide binding	0.114848452385726	bayes_pls_golite062009
purine nucleotide binding	0.10713474095071	bayes_pls_golite062009
purine ribonucleotide binding	0.0916519414118148	bayes_pls_golite062009
ribonucleotide binding	0.0915913646217636	bayes_pls_golite062009
3'-5' DNA helicase activity	0.0769112340058804	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2