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Protein Overview: RAD5

General Information:
Name(s) found: RAD5 / YLR032W [SGD]
Description(s) found:
Found 26 descriptions.
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  • DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1169 amino acids

    Gene Ontology:
    Cellular Component: nuclear chromatin [IDALink to PubMed article]
    Biological Process: DNA repair [IMPLink to PubMed article]
    telomere maintenance [IMPLink to PubMed article]
    double-strand break repair [IGILink to PubMed article][IMPLink to PubMed article]
    Molecular Function: ATPase activity [TASLink to PubMed article]
    single-stranded DNA binding [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSHIEQEERK RFFNDDLDTS ETSLNFKSEN KESFLFANSH NDDDDDVVVS VSDTTEGEGD  60
   61 RSIVPVRREI EEEGQNQFIT ELLRIIPEMP KDLVMELNEK FGSQEEGLSL ALSHYFDHNS 120
  121 GTSISKIPSS PNQLNTLSDT SNSTLSPSSF HPKRRRIYGF RNQTRLEDKV TWKRFIGALQ 180
  181 VTGMATRPTV RPLKYGSQMK LKRSSEEISA TKVYDSRGRK KASMASLVRI FDIQYDREIG 240
  241 RVSEDIAQIL YPLLSSHEIS FEVTLIFCDN KRLSIGDSFI LQLDCFLTSL IFEERNDGES 300
  301 LMKRRRTEGG NKREKDNGNF GRTLTETDEE LESRSKRLAL LKLFDKLRLK PILDEQKALE 360
  361 KHKIELNSDP EIIDLDNDEI CSNQVTEVHN NLRDTQHEEE TMNLNQLKTF YKAAQSSESL 420
  421 KSLPETEPSR DVFKLELRNY QKQGLTWMLR REQEFAKAAS DGEASETGAN MINPLWKQFK 480
  481 WPNDMSWAAQ NLQQDHVNVE DGIFFYANLH SGEFSLAKPI LKTMIKGGIL SDEMGLGKTV 540
  541 AAYSLVLSCP HDSDVVDKKL FDIENTAVSD NLPSTWQDNK KPYASKTTLI VVPMSLLTQW 600
  601 SNEFTKANNS PDMYHEVYYG GNVSSLKTLL TKTKTPPTVV LTTYGIVQNE WTKHSKGRMT 660
  661 DEDVNISSGL FSVNFYRIII DEGHNIRNRT TVTSKAVMAL QGKCKWVLTG TPIINRLDDL 720
  721 YSLVKFLELD PWRQINYWKT FVSTPFESKN YKQAFDVVNA ILEPVLLRRT KQMKDKDGKP 780
  781 LVELPPKEVV IKRLPFSKSQ DLLYKFLLDK AEVSVKSGIA RGDLLKKYST ILVHILRLRQ 840
  841 VCCHPGLIGS QDENDEDLSK NNKLVTEQTV ELDSLMRVVS ERFDNSFSKE ELDAMIQRLK 900
  901 VKYPDNKSFQ SLECSICTTE PMDLDKALFT ECGHSFCEKC LFEYIEFQNS KNLGLKCPNC 960
  961 RNQIDACRLL ALVQTNSNSK NLEFKPYSPA SKSSKITALL KELQLLQDSS AGEQVVIFSQ1020
 1021 FSTYLDILEK ELTHTFSKDV AKIYKFDGRL SLKERTSVLA DFAVKDYSRQ KILLLSLKAG1080
 1081 GVGLNLTCAS HAYMMDPWWS PSMEDQAIDR LHRIGQTNSV KVMRFIIQDS IEEKMLRIQE1140
 1141 KKRTIGEAMD TDEDERRKRR IEEIQMLFE

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Krogan NJ, et al. (2006) ALT2 RAD5 RRP1
    View Details Qiu et al. (2008) PCC1 RAD18 RAD5 RAP1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run ASF1 3829 - low filter Green EM, et al (2005)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RAD5 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..378] MSA 5.209996 View MSA. No confident structure predictions are available.
    2 View Details [379..476] ORFEUS 11.29 RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
    3 View Details [477..819] ORFEUS 11.29 RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
    4 View Details [820..921] MSA 1.345993 View MSA. No confident structure predictions are available.
    5 View Details [922..975] deduced N/A Confident ab initio structure predictions are available.
    6 View Details [976..1169] PSI-BLAST 2.522879 Nucleotide excision repair enzyme UvrB

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3 No functions predicted.
    4
    Term Confidence Notes
  • DNA-dependent ATPase activity
    		•  2.72462789867829 bayes_pls_golite062009
  • DNA helicase activity
    		•  2.2817164193758 bayes_pls_golite062009
  • binding
    		•  2.05253348982328 bayes_pls_golite062009
  • ATP-dependent DNA helicase activity
    		•  1.88455339138556 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  1.84050240798436 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  1.81138802583782 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides
    		•  1.78927528875848 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
    		•  1.78903889318615 bayes_pls_golite062009
  • helicase activity
    		•  1.70242731830224 bayes_pls_golite062009
  • microtubule motor activity
    		•  1.35938517416356 bayes_pls_golite062009
  • motor activity
    		•  1.32129340699286 bayes_pls_golite062009
  • protein-DNA loading ATPase activity
    		•  1.29640539057136 bayes_pls_golite062009
  • hydrolase activity
    		•  1.29419296935491 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.2539059700735 bayes_pls_golite062009
  • purine NTP-dependent helicase activity
    		•  1.19311444180077 bayes_pls_golite062009
  • ATP-dependent helicase activity
    		•  1.19311444180077 bayes_pls_golite062009
  • ATPase activity
    		•  1.18431260772292 bayes_pls_golite062009
  • DNA-directed DNA polymerase activity
    		•  1.17617511072239 bayes_pls_golite062009
  • DNA polymerase activity
    		•  1.14104131900023 bayes_pls_golite062009
  • DNA clamp loader activity
    		•  1.12431517057397 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  1.117585097627 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.08697924060619 bayes_pls_golite062009
  • catalytic activity
    		•  1.00104173493322 bayes_pls_golite062009
  • DNA binding
    		•  0.702147676354075 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  0.568457189190858 bayes_pls_golite062009
  • mismatched DNA binding
    		•  0.31144582840038 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.287777990975748 bayes_pls_golite062009
  • DNA strand annealing activity
    		•  0.283577486331079 bayes_pls_golite062009
  • nucleotide binding
    		•  0.114848452385726 bayes_pls_golite062009
  • purine nucleotide binding
    		•  0.10713474095071 bayes_pls_golite062009
  • purine ribonucleotide binding
    		•  0.0916519414118148 bayes_pls_golite062009
  • ribonucleotide binding
    		•  0.0915913646217636 bayes_pls_golite062009
  • 3'-5' DNA helicase activity
    		•  0.0769112340058804 bayes_pls_golite062009
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.97

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle