Protein Overview: BRE2

General Information:
Name(s) found:	BRE2 / YLR015W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323043\|ref\|NP_013115.1\| Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; involved in telomere maintenance; similar to trithorax-group protein ASH2L; Bre2p [Sac [NCBI NR] gi\|2506766\|sp\|P43132.2\|BRE2_YEAST RecName: Full=COMPASS component BRE2; AltName: Full=Complex proteins associated with SET1 protein BRE2; AltName: Full=Set1C component BRE2; AltName: Full=Brefeldin-A sensitivity protein 2 [NCBI NR] pir\|\|S64837 hypothetical protein YLR015w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1360314\|emb\|CAA97537.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] (P43132) Hypothetical 58.3 kDa protein in PPR1-SNF7 intergenic region (ORFX) [Swiss-Prot] COMPASS component BRE2 OS=Saccharomyces cerevisiae GN=BRE2 PE=1 SV=2 [Swiss-Prot] BRE2 Subunit of the COMPASS complex, which methylates histone H3 on lysine 4 [mips] Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L [SGD] BRE2, Chr XII from 175226-176743 [yeast_orfs2] Chr XII from 175226-176743 [yeastorf3.fasta] BRE2 SGDID:S0004005, Chr XII from 175226-176743, Verified ORF [yeastflysequest.fasta] Bre2p [Saccharomyces cerevisiae]gi\|1360314\|emb\|CAA97537.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|2506766\|sp\|P43132\|YL1R_YEAST Hypothetical 58.3 kDa protein in PPR1-SNF7 intergenic region (ORFX)gi\|2131748\|pir\|\|S64837 hypothetical protein [nrdb_11152004_Chlamydomonas] BRE2 SGDID:S000004005, Chr XII from 175226-176743, Verified ORF, "Subunit of the COMPASS (Set1C) complex, which methylates Rad6p ubiquitinated histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; has similarity to the tritho [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] BRE2 SGDID:S000004005, Chr XII from 175226-176743, Verified ORF, "Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; involved in telomere maintenance; similar to tri [yeast-contam.fasta] COMPASS component BRE2 (Complex proteins associated with SET1 protein BRE2) (Set1C component BRE2) (Brefeldin-A sensitivity protein 2) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; involved in telomere maintenance; similar to trithorax-group protein ASH2L; Bre2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] BRE2 SGDID:S000004005, Chr XII from 175226-176743, Verified ORF, "Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] BRE2 SGDID:S000004005, Chr XII from 175226-176743, Verified ORF, "Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; involved in telomere maintenance; similar to trithorax-group protein ASH2L" [yeast-bovrabprots.fasta] [chemostatdb.fasta] BRE2 SGDID:S000004005, Chr XII from 175226-176743, Verified ORF, "Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L" [SGDyeast.20101221.fasta] COMPASS component BRE2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BRE2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] BRE2 SGDID:S000004005, Chr XII from 175227-176744, Genome Release 64-1-1, Verified ORF, "Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L" [yeast-030211-contam.fasta] BRE2 SGDID:S000004005, Chr XII from 175227-176744, Genome Release 64-2-1, Verified ORF, "Subunit of COMPASS (Set1C) complex; COMPASS methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L" [20141208_orf_trans.fasta] YLR015W SGDID:S000004005, Chr XII from 175227-176744, Genome Release 64-1-1, Verified ORF, "Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	505 amino acids
Gene Ontology:
Cellular Component:	Set1C/COMPASS complex [IPI ]
Biological Process:	transcription [ISS] histone methylation [IDA ][IMP ] telomere maintenance [IMP] chromatin silencing at telomere [IMP ]
Molecular Function:	histone methyltransferase activity (H3-K4 specific) [IDA ][IMP ] transcription regulator activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKLGIIPYQE GTDIVYKNAL QGQQEGKRPN LPQMEATHQI KSSVQGTSYE FVRTEDIPLN  60
   61 RRHFVYRPCS ANPFFTILGY GCTEYPFDHS GMSVMDRSEG LSISRDGNDL VSVPDQYGWR 120
  121 TARSDVCIKE GMTYWEVEVI RGGNKKFADG VNNKENADDS VDEVQSGIYE KMHKQVNDTP 180
  181 HLRFGVCRRE ASLEAPVGFD VYGYGIRDIS LESIHEGKLN CVLENGSPLK EGDKIGFLLS 240
  241 LPSIHTQIKQ AKEFTKRRIF ALNSHMDTMN EPWREDAENG PSRKKLKQET TNKEFQRALL 300
  301 EDIEYNDVVR DQIAIRYKNQ LFFEATDYVK TTKPEYYSSD KRERQDYYQL EDSYLAIFQN 360
  361 GKYLGKAFEN LKPLLPPFSE LQYNEKFYLG YWQHGEARDE SNDKNTTSAK KKKQQQKKKK 420
  421 GLILRNKYVN NNKLGYYPTI SCFNGGTARI ISEEDKLEYL DQIRSAYCVD GNSKVNTLDT 480
  481 LYKEQIAEDI VWDIIDELEQ IALQQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BRE2 SDC1 SET1 SHG1 SPP1 SWD1 SWD2 SWD3
View Details	Krogan NJ, et al. (2006)		BRE2 ERF2 SDC1 SET1 SHG1 SPP1 SWD1 SWD3 YHI9 YLL023C
View Details	Gavin AC, et al. (2006)		BRE2 SDC1 SET1 SPP1 SWD1 SWD3
View Details	Gavin AC, et al. (2002)		BRE2 DOA4 RSM7 SDC1 SET1 SPP1 SSZ1 SWD1 SWD3
View Details	Ho Y, et al. (2002)		BRE2 SET1
View Details	Qiu et al. (2008)		BRE2 SDC1 SET1 SHG1 SPP1 SWD1 SWD2 SWD3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NAF1	BRE2	1	Hazbun TR, et al. (2003)
View Screen	UBC7	BRE2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BRE2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..275]	MSA	3.009939	View MSA. No confident structure predictions are available.
2	View Details	[276..505]	deduced	N/A	No confident structure predictions are available.
3	View Details	[293..505]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
binding	2.36160381896665	bayes_pls_golite062009
transcription regulator activity	2.17920418295591	bayes_pls_golite062009
nucleic acid binding	1.9830722589036	bayes_pls_golite062009
DNA binding	1.82525586215701	bayes_pls_golite062009
transcription repressor activity	1.26658862258675	bayes_pls_golite062009
transcription factor activity	1.16784442642933	bayes_pls_golite062009
protein binding	0.729859333703778	bayes_pls_golite062009
protein kinase activity	0.358328289529296	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.155087758481234	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.020584443202555	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2