Protein Overview: BUD2

General Information:
Name(s) found:	BUD2 / YKL092C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322758\|ref\|NP_012831.1\| GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; Bud2p [Saccharomyces cerevisiae] [NCBI NR] gi\|543907\|sp\|P33314.2\|BUD2_YEAST RecName: Full=Inhibitory regulator protein BUD2/CLA2; AltName: Full=Bud site selection protein 2 [NCBI NR] gi\|486139\|emb\|CAA81930.1\| BUD2 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S36773 GTPase-activating protein BUD2 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|450075\|prf\|\|1920367A GTPase-activating protein [NCBI NR] gi\|402734\|gb\|AAA34461.1\| GTPase-activating protein [Saccharomyces cerevisiae] [NCBI NR] Inhibitory regulator protein BUD2/CLA2 OS=Saccharomyces cerevisiae GN=BUD2 PE=1 SV=2 [Swiss-Prot] BUD2 GTPase-activating protein for Bud1p/Rsr1p [mips] GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types [SGD] BUD2, Chr XI from 265787-269101, reverse complement [yeast_orfs2] Chr XI from 265787-269101, reverse complement [yeastorf3.fasta] Chr XI from 265786-269100, reverse complement [yeastorf4.fasta] Chr XI from 269100-265786, reverse complement [yeastorf5.fasta] (P33314) Inhibitory regulator protein BUD2/CLA2 [4932.FASTACCERV.txt] BUD2 SGDID:S0001575, Chr XI from 269104-265790, reverse complement, Verified ORF [yeastflysequest.fasta] Bud2p [Saccharomyces cerevisiae]gi\|486139\|emb\|CAA81930.1\| BUD2 [Saccharomyces cerevisiae]gi\|480378\|pir\|\|S36773 GTPase-activating protein BUD2 - yeast (Saccharomyces cerevisiae)gi\|543907\|sp\|P33314\|BUD2_YEAST Inhibitory regulator protein BUD2/CLA2gi\|402 [nrdb_11152004_Chlamydomonas] BUD2 SGDID:S000001575, Chr XI from 269103-265789, reverse complement, Verified ORF, "GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] BUD2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GTPase-activating protein [nr-271106-contam.fasta] GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; Bud2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Inhibitory regulator protein BUD2/CLA2 [nr-271106-contam.fasta] [chemostatdb.fasta] Inhibitory regulator protein BUD2/CLA2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BUD2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] BUD2 SGDID:S000001575, Chr XI from 269459-266145, Genome Release 64-1-1, reverse complement, Verified ORF, "GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types" [yeast-030211-contam.fasta] BUD2 SGDID:S000001575, Chr XI from 269459-266145, Genome Release 64-2-1, reverse complement, Verified ORF, "GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types" [20141208_orf_trans.fasta] TR5815\|c0_g1_i1\|g.4022 ORF TR5815\|c0_g1_i1\|g.4022 TR5815\|c0_g1_i1\|m.4022 type:complete len:1105 (+) TR5815\|c0_g1_i1:101-3415(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YKL092C SGDID:S000001575, Chr XI from 269459-266145, Genome Release 64-1-1, reverse complement, Verified ORF, "GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_6482\|g.5034 ORF asmbl_6482\|g.5034 asmbl_6482\|m.5034 type:complete len:1105 (+) asmbl_6482:100-3414(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_6503\|g.4109 ORF ID=asmbl_6503\|g.4109 ID=asmbl_6503\|m.4109 type:complete len:1105 (+) ID=asmbl_6503:100-3414(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1104 amino acids
Gene Ontology:
Cellular Component:	intracellular [TAS]
Biological Process:	filamentous growth [IMP] axial cellular bud site selection [TAS ] cellular bud site selection [TAS] bipolar cellular bud site selection [TAS ] small GTPase mediated signal transduction [TAS]
Molecular Function:	GTPase activator activity [TAS ] Ras GTPase activator activity [TAS] signal transducer activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSNNEPAQS RTSYFKLNEF LSNVKHYKNT FKGEIQWCNN LSLNDWKTHY LQITSTGALT  60
   61 HSIDELTADS TNIQPIIKHL QQCRIEIIKD KHSSFKDINA NCNFIIQVNT SGKDNKVYLR 120
  121 VKSWSDFKKL LTCLIWWSSM KTNGIFNKFQ VSRPLEFKSK KMAKPESLLV YKLNVFGPIV 180
  181 KNIVLPPATN ILESPDIINN DDNSVGWFSA MGVLKSNGML DLLLQSDGSL IYSLNISQLL 240
  241 RSEIRILDSS VLQSENSLFL GELPLLRSQL GLEKFRIENI ASAATNSSDI SQEIIVEFPL 300
  301 RIDLEDCFIA LQSFARSEYL SITGSDKSND MKISNSFKIS ILEANFQSIN LNDKNNTPWS 360
  361 IFTDITAWGH TWARTSMVSN SSNPFWREEF QFNELLRLTN SYLEIKQLFH DLNNKKRLRL 420
  421 IGKIKITQEI INDTRYNKET RLPIMDVDNK NFQIGTICIK ISSNLNFILP STNFVKLEKL 480
  481 LMNANLSMVS NLIYKSSSSM ENDNKLTQTS IIFLDIFQSL SRIEEWFHVL IDKELAKIDG 540
  541 TVSRINQKNL DSKHVFNSLF RGNSILTKSI EQYFFRVGNE YLSKALSAIL KEIIESNKSC 600
  601 ELDPARVKEK DEVKKRKIIA DNYKRLYSWV TKIWKRLYAT SNDLPIEIRN VLKIFRQKLE 660
  661 IICIDDTLQI ILNGISGLLF LRFFCPVILN PKLFKYVSQN LNETARRNLT LISKVLLNLS 720
  721 TLTQFANKEP WLMKMNNFID KRHNDLLDYI DKMTQKKLDF NSKILNLSST ISRPKLAIEQ 780
  781 TMLDDLPQIP YLLDKNLRET EFVNLIVNFS QEDMTKMEKY NHMDNGGKGE LIEEEGLLSG 840
  841 SSLNLSVDKK DLDSPIEVKP EIGELEFEKI TENNTEIFGD DLMNLLKSDD VGSRSRDLDN 900
  901 GANSGIKFNS IIPKAEEEKH AMKELEQESC LLYNRINHIR KRLSGYECAS STLFEDKKYS 960
  961 ISLSHKIFYE EIKEGKEIVL KLLNKPTNEN SSARLQKFFT KGVSSKSNNT VGDSYCKFLT1020
 1021 IDVSDENPKS SNKTSVHGTS SENGAKDDYL TLPNSQGKGN LGNRFSPTKL SRIMRKPPNA1080
 1081 DVPKEQNSRK LTRWFKKKKE TGGS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BUD2 MBP1 MSA2 SWI4 SWI6 WHI5
View Details	Gavin AC, et al. (2006)		BUD2 CCP1 MRP8 PDR5 PET9 PTM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RBA50	No Comments	Hazbun TR, et al. (2003)
View Run	PAN1	No Comments	Toshima J, et al (2006)
View Run	CLB2	No Comments	McCusker D, et al (2007)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BUD2	PRM5	2	Unpublished Fields Lab Data
View Screen	BUD2	EEB1	2	Unpublished Fields Lab Data
View Screen	BUD2	CLN2	2	Drees BL, et al. (2001)
View Screen	ARF3	BUD2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BUD2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..194]	deduced	N/A	No confident structure predictions are available.
2	View Details	[195..316]	PSI-BLAST	59.522879	Synaptogamin I
3	View Details	[317..466]	PSI-BLAST	59.522879	Synaptogamin I
4	View Details	[467..535] [759..827]	PSI-BLAST	271.68867	p120GAP domain
5	View Details	[536..758]	PSI-BLAST	271.68867	p120GAP domain
6	View Details	[828..1104]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
GTPase regulator activity	5.23789256375467	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	5.21562004568462	bayes_pls_golite062009
small GTPase regulator activity	4.91645234075732	bayes_pls_golite062009
GTPase activator activity	4.81440293338491	bayes_pls_golite062009
Ras GTPase activator activity	4.76400460221691	bayes_pls_golite062009
Rho GTPase activator activity	4.58620967349979	bayes_pls_golite062009
enzyme regulator activity	4.5322075775333	bayes_pls_golite062009
enzyme activator activity	4.29367232260052	bayes_pls_golite062009
Rac GTPase activator activity	3.29568423512865	bayes_pls_golite062009
binding	1.62780792681766	bayes_pls_golite062009
protein binding	1.21496582666968	bayes_pls_golite062009
GTPase binding	0.240141218420366	bayes_pls_golite062009
small GTPase binding	0.0713778754122761	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2