Protein Overview: SLH1

General Information:
Name(s) found:	SLH1 / YGR271W [SGD]
Description(s) found: Found 19 descriptions. SHOW ALL	Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1967 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	regulation of translation [IGI ][IMP ]
Molecular Function:	RNA helicase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTEYSADSS KSFMIAMQSM IDTSQTFNLD RSKISLPDFD DELKKVQKDE PNQRTELTVL  60
   61 SQDRNDWDDI FEEFKDISFA QLQSIIDSYK TKNAVAVYKK IGKLINEAET TLSSNVLLET 120
  121 VLQMVYKHQK QELEKELLDF LGTGNIDLVS LLLQHRRMIV ATPIETTILL IKNAVNSTPE 180
  181 FLTQQDIRNQ VLESAEDAKN RKLNPATKII KYPHVFRKYE AGSTTAMAFA GQKFTLPVGT 240
  241 TRMSYNTHEE IIIPAADQAS NKNYLYTKLL KISDLDHFCK TVFPYETLNQ IQSLVYPVAY 300
  301 KTNENMLICA PTGAGKTDIA LLTIINTIKQ FSVVNGENEI DIQYDDFKVI YVAPLKALAA 360
  361 EIVDKFSKKL APFNIQVREL TGDMQLTKAE ILATQVIVTT PEKWDVVTRK ANGDNDLVSK 420
  421 VKLLIIDEVH LLHEDRGPVI ETLVARTLRQ VESSQSMIRI IGLSATLPNF MDVADFLGVN 480
  481 RQIGMFYFDQ SFRPKPLEQQ LLGCRGKAGS RQSKENIDKV AYDKLSEMIQ RGYQVMVFVH 540
  541 SRKETVKSAR NFIKLAESNH EVDLFAPDPI EKDKYSRSLV KNRDKDMKEI FQFGFGIHHA 600
  601 GMARSDRNLT EKMFKDGAIK VLCCTATLAW GVNLPADCVI IKGTQVYDSK KGGFIDLGIS 660
  661 DVIQIFGRGG RPGFGSANGT GILCTSNDRL DHYVSLITQQ HPIESRFGSK LVDNLNAEIS 720
  721 LGSVTNVDEA IEWLGYTYMF VRMRKNPFTY GIDWEEIAND PQLYERRRKM IVVAARRLHA 780
  781 LQMIVFDEVS MHFIAKDLGR VSSDFYLLNE SVEIFNQMCD PRATEADVLS MISMSSEFDG 840
  841 IKFREEESKE LKRLSDESVE CQIGSQLDTP QGKANVLLQA YISQTRIFDS ALSSDSNYVA 900
  901 QNSVRICRAL FLIGVNRRWG KFSNVMLNIC KSIEKRLWAF DHPLCQFDLP ENIIRRIRDT 960
  961 KPSMEHLLEL EADELGELVH NKKAGSRLYK ILSRFPKINI EAEIFPITTN VMRIHIALGP1020
 1021 DFVWDSRIHG DAQFFWVFVE ESDKSQILHF EKFILNRRQL NNQHEMDFMI PLSDPLPPQV1080
 1081 VVKVVSDTWI GCESTHAISF QHLIRPFNET LQTKLLKLRP LPTSALQNPL IESIYPFKYF1140
 1141 NPMQTMTFYT LYNTNENAFV GSPTGSGKTI VAELAIWHAF KTFPGKKIVY IAPMKALVRE1200
 1201 RVDDWRKKIT PVTGDKVVEL TGDSLPDPKD VHDATIVITT PEKFDGISRN WQTRKFVQDV1260
 1261 SLIIMDEIHL LASDRGPILE MIVSRMNYIS SQTKQPVRLL GMSTAVSNAY DMAGWLGVKD1320
 1321 HGLYNFPSSV RPVPLKMYID GFPDNLAFCP LMKTMNKPVF MAIKQHSPDK PALIFVASRR1380
 1381 QTRLTALDLI HLCGMEDNPR RFLNIDDEEE LQYYLSQVTD DTLKLSLQFG IGLHHAGLVQ1440
 1441 KDRSISHQLF QKNKIQILIA TSTLAWGVNL PAHLVIIKGT QFFDAKIEGY RDMDLTDILQ1500
 1501 MMGRAGRPAY DTTGTAIVYT KESKKMFYKH FLNVGFPVES SLHKVLDDHL GAEITSGSIT1560
 1561 NKQEALDFLS WTFLFRRAHH NPTYYGIEDD TSTAGVSEHL SSLIDSTLEN LRESQCVLLH1620
 1621 GDDIVATPFL SISSYYYISH LTIRQLLKQI HDHATFQEVL RWLSLAVEYN ELPVRGGEII1680
 1681 MNEEMSQQSR YSVESTFTDE FELPMWDPHV KTFLLLQAHL SRVDLPIADY IQDTVSVLDQ1740
 1741 SLRILQAYID VASELGYFHT VLTMIKMMQC IKQGYWYEDD PVSVLPGLQL RRIKDYTFSE1800
 1801 QGFIEMTPQQ KKKKLLTLEE IGRFGYKKLL NVFDQLTFGM TESEDTKKRF VSVCQRLPVL1860
 1861 EGMKFEEQEN NEVLTFYSKH LSSKHNNGFE VYCDKFPKIQ KELWFLIGHK GDELLMIKRC1920
 1921 QPKQMNKEVI IHCDLFIPEE IRGEELQFSL INDALGLRYD MVHKLIS

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	KAP104	SLH1	1	Unpublished Fields Lab Data
View Screen	UBC7	SLH1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	SLH1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..255]	MSA	10.589997	View MSA. No confident structure predictions are available.
2	View Details	[256..492]	PSI-BLAST	80.69897	Putative DEAD box RNA helicase
3	View Details	[493..695]	PSI-BLAST	80.69897	Putative DEAD box RNA helicase
4	View Details	[696..789]	PSI-BLAST	4.39794	Nucleotide excision repair enzyme UvrB
5	View Details	[790..1074]	MSA	1.091995	View MSA. No confident structure predictions are available.
6	View Details	[1075..1270]	ORFEUS	14.93	RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
7	View Details	[1271..1588]	PSI-BLAST	6.0	Nucleotide excision repair enzyme UvrB
8	View Details	[1589..1776]	deduced	N/A	No confident structure predictions are available.
9	View Details	[1777..1856]	PSI-BLAST	2.522879	Ribosomal protein L32e
10	View Details	[1857..1967]	MSA	1.038994	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
	6.33745214454795	bayes_pls_golite062009
RNA-dependent ATPase activity	2.97534133779092	bayes_pls_golite062009
ATP-dependent RNA helicase activity	2.96650679245363	bayes_pls_golite062009
RNA helicase activity	2.83814950186361	bayes_pls_golite062009
structural molecule activity	2.81579918518889	bayes_pls_golite062009
binding	2.59063189683655	bayes_pls_golite062009
nucleic acid binding	1.89039798688851	bayes_pls_golite062009
helicase activity	1.52111969749634	bayes_pls_golite062009
ATP-dependent helicase activity	1.48276729246798	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.48276729246798	bayes_pls_golite062009
protein binding	1.40069187357924	bayes_pls_golite062009
cytoskeletal protein binding	0.262645824445581	bayes_pls_golite062009
ligand-gated ion channel activity	0.0135923951994963	bayes_pls_golite062009
ligand-gated channel activity	0.0135923951994963	bayes_pls_golite062009

Term	Confidence	Notes
	4.704140727858	bayes_pls_golite062009
structural constituent of ribosome	3.070470376968	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
RNA binding	2.29590582680214	bayes_pls_golite062009
nucleic acid binding	2.11222585187695	bayes_pls_golite062009
structural molecule activity	2.08211451183607	bayes_pls_golite062009
DNA binding	1.7904783386531	bayes_pls_golite062009
transcription regulator activity	1.68903321260301	bayes_pls_golite062009
RNA helicase activity	1.42791449104435	bayes_pls_golite062009
RNA-dependent ATPase activity	1.39335733374328	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.36724390116598	bayes_pls_golite062009
snRNA binding	1.20045983086701	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
mRNA binding	0.76699944561918	bayes_pls_golite062009
helicase activity	0.6191010101321	bayes_pls_golite062009
transcription factor activity	0.603758349693077	bayes_pls_golite062009
purine nucleotide binding	0.569910969126728	bayes_pls_golite062009
purine ribonucleotide binding	0.568840867400671	bayes_pls_golite062009
ribonucleotide binding	0.568779924702229	bayes_pls_golite062009
nucleotide binding	0.564275755355315	bayes_pls_golite062009
purine NTP-dependent helicase activity	0.29664013896754	bayes_pls_golite062009
ATP-dependent helicase activity	0.29664013896754	bayes_pls_golite062009
translation regulator activity	0.29096092258568	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.274994961137851	bayes_pls_golite062009
ATP binding	0.1860481660648	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
adenyl ribonucleotide binding	0.152859582615242	bayes_pls_golite062009
adenyl nucleotide binding	0.138615225841109	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0530667986761242	bayes_pls_golite062009
pyrophosphatase activity	0.0482442222493492	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.028470639373934	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.0265151533232588	bayes_pls_golite062009

Term	Confidence	Notes
structural molecule activity	2.80935217964088	bayes_pls_golite062009
binding	2.65544526722793	bayes_pls_golite062009
protein binding	1.50286160643298	bayes_pls_golite062009
ligand-gated ion channel activity	0.423207248971435	bayes_pls_golite062009
ligand-gated channel activity	0.423207248971435	bayes_pls_golite062009
cytoskeletal protein binding	0.42238661903557	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2