Identification of gene encoding a putative RNA-helicase, homologous to SKI2, in chromosome VII of Saccharomyces cerevisiae

Yeast. 1997 Mar 30;13(4):391-7. doi: 10.1002/(SICI)1097-0061(19970330)13:4<391::AID-YEA92>3.0.CO;2-Q.

Abstract

We have determined the nucleotide sequence of a segment of VII of the yeast Saccharomyces cerevisiae contained in the cosmid clone pEGH101 for a total of 7 kbp. This sequence contains a large open reading frame (ORF) called G9365, coding for a protein of 1967 amino acids that shows a significant homology with the product of the SKI2 gene of S. cerevisiae and contains domains characteristics of RNA-helicases. The ORF is transcribed in vegetative cells but it is not essential for viability as demonstrated by gene disruption.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromosomes, Fungal / genetics*
  • Fungal Proteins / genetics*
  • Genes, Fungal / genetics*
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Phenotype
  • RNA Helicases
  • RNA Nucleotidyltransferases / genetics*
  • RNA, Double-Stranded / analysis
  • RNA, Fungal / analysis
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Spores, Fungal
  • Transcription, Genetic

Substances

  • Fungal Proteins
  • RNA, Double-Stranded
  • RNA, Fungal
  • SKI2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • RNA Nucleotidyltransferases
  • RNA Helicases

Associated data

  • GENBANK/U35242
  • SWISSPROT/P15938
  • SWISSPROT/P24783
  • SWISSPROT/P35207