Protein Overview: RMD8

General Information:
Name(s) found:	RMD8 / YFR048W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|836803\|dbj\|BAA09287.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|22532998\|ref\|NP_683716.1\| Cytosolic protein required for sporulation; Rmd8p [Saccharomyces cerevisiae] [NCBI NR] gi\|1176024\|sp\|P43620.1\|RMD8_YEAST RecName: Full=Sporulation protein RMD8; AltName: Full=Required for meiotic nuclear division protein 8 [NCBI NR] pir\|\|S56303 probable membrane protein YFR048w - yeast (Saccharomyces cerevisiae) [NCBI NR] (P43620) Hypothetical 75.9 kDa protein in SAP155-YMR31 intergenic region [Swiss-Prot] Sporulation protein RMD8 OS=Saccharomyces cerevisiae GN=RMD8 PE=1 SV=1 [Swiss-Prot] RMD8 Cytosolic protein required for sporulation [mips] Cytosolic protein required for sporulation [SGD] YFR048W, Chr VI from 246133-248121 [yeast_orfs2] Chr VI from 246133-248121 [yeastorf3.fasta] RMD8 SGDID:S0001944, Chr VI from 246133-248121, Verified ORF [yeastflysequest.fasta] Cytosolic protein required for sporulation [Saccharomyces cerevisiae]gi\|1176024\|sp\|P43620\|YFL8_YEAST Hypothetical 75.9 kDa protein in SAP155-YMR31 intergenic regiongi\|1084762\|pir\|\|S56303 probable membrane protein YFR048w - yeast (Saccharomyces cerevisia [nrdb_11152004_Chlamydomonas] RMD8 SGDID:S000001944, Chr VI from 246133-248121, Verified ORF, "Cytosolic protein required for sporulation" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Sporulation protein RMD8 [nr-271106-contam.fasta] Cytosolic protein required for sporulation; Rmd8p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Sporulation protein RMD8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RMD8 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RMD8 SGDID:S000001944, Chr VI from 246146-248134, Genome Release 64-1-1, Verified ORF, "Cytosolic protein required for sporulation" [yeast-030211-contam.fasta] RMD8 SGDID:S000001944, Chr VI from 246146-248134, Genome Release 64-2-1, Verified ORF, "Cytosolic protein required for sporulation" [20141208_orf_trans.fasta] YFR048W SGDID:S000001944, Chr VI from 246146-248134, Genome Release 64-1-1, Verified ORF, "Cytosolic protein required for sporulation" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	662 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	response to drug [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSYKANQPSP GEMPKRSPSI LVTDARTSKN RMSAPFAGHA AGSRKNMENA GVTKSQGVRS  60
   61 SAIGPSPLQS FTHPRRRSSG RFSDISIDNI LSDNSDIPSA RREERLSSSS SDRPRQYERL 120
  121 SSRRKMINPL PPRTSKTSQK LVLIPEDDNL NHFQTLPTNA LDRQRPKVGS MKSNSFDRLP 180
  181 RYSKEKSMAR ITAYNVADGF NLNQLYKFLQ ETHEVSPRLY DECLYVAYTL PLLPGKGGFR 240
  241 IKSNLSKKTM GGKTLIDNLI DTSEQRDHHY EYYSGVETVE DANNNYELET SGNNNNANQD 300
  301 TTTVPDHLPN PVGQQDSFNP MEPQFFAEET PLEIEKRERT ERINMLKKEE NDSDASCGND 360
  361 NNNKNNDKSK LYAVEGNDQY VQSSRSPASP SSISTPSPPS SSQNDFDRVY KMHRDNDHEG 420
  421 NDRHAEIFIF HYGVIVFWNF TEIQEKNILG DITFADYKNL MIRPLDEQDI ETEQFHFEYD 480
  481 RDTERPRIFN DIVTLRSGDH IIELTLSHAI AQSSKLSRFE SRISPILISV TKLPKRLALY 540
  541 GTLGLKREQL LKKSGKLFKL RVDVNLSSTI LDTPEFFWSF EPSLHPLYVA MREYLEIDQR 600
  601 VQVLNDRCKV FLEFFDICVD SVAERNMARV TWWFILVILF GVIFSLTEIF VRYVIIHRHT 660
  661 ST

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		AHA1 BUD3 CNS1 CPR6 DIG2 DNM1 DSF1, YNR073C FRT2 GPA2 HOM6 HSC82 HSP104 HSP82 MBB1 MRPL50 PAM1 PPT1 QNS1 RMD8 RTS1 SMT3 STI1 STP4 TDH1 UTP20 YBL104C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RRB1	RMD8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RMD8	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..124]	deduced	N/A	No confident structure predictions are available.
2	View Details	[125..274]	MSA	1.002966	View MSA. No confident structure predictions are available.
3	View Details	[275..359]	deduced	N/A	No confident structure predictions are available.
4	View Details	[360..662]	Pfam	148.091515	Uncharacterised ACR, YagE family COG1723 No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2