Protein Overview: VPS3

General Information:
Name(s) found:	VPS3 / YDR495C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|927765\|gb\|AAB64937.1\| Vps3p: Vacuolar sorting protein [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S11177 vacuolar sorting protein VPS3 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6320703\|ref\|NP_010783.1\| Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase; Vps3p [Saccharomyces [NCBI NR] gi\|4782\|emb\|CAA37865.1\| VPS3 [Saccharomyces cerevisiae] [NCBI NR] gi\|139414\|sp\|P23643.1\|VPS3_YEAST RecName: Full=Vacuolar protein sorting-associated protein 3; AltName: Full=Vacuolar protein-targeting protein 17 [NCBI NR] Vacuolar protein sorting-associated protein 3 OS=Saccharomyces cerevisiae GN=VPS3 PE=1 SV=1 [Swiss-Prot] VPS3 Vacuolar sorting protein [mips] Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase [SGD] VPS3, Chr IV from 1438118-1441153, reverse complement [yeast_orfs2] Chr IV from 1438118-1441153, reverse complement [yeastorf3.fasta] Chr IV from 1438101-1441136, reverse complement [yeastorf4.fasta] Chr IV from 1441138-1438103, reverse complement [yeastorf5.fasta] (P23643) Vacuolar protein sorting-associated protein VPS3 [4932.FASTACCERV.txt] VPS3 SGDID:S0002903, Chr IV from 1441138-1438103, reverse complement, Verified ORF [yeastflysequest.fasta] VPS3 SGDID:S0002903, Chr IV from 1441140-1438105, reverse complement, Verified ORF [10112005cervSGD.fasta] Vacuolar sorting protein [Saccharomyces cerevisiae]gi\|927765\|gb\|AAB64937.1\| Vps3p: Vacuolar sorting protein [Saccharomyces cerevisiae]gi\|4782\|emb\|CAA37865.1\| VPS3 [Saccharomyces cerevisiae]gi\|83608\|pir\|\|S11177 vacuolar sorting protein VPS3 - yeast (Sac [nrdb_11152004_Chlamydomonas] VPS3 SGDID:S000002903, Chr IV from 1441140-1438105, reverse complement, Verified ORF, "Cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] VPS3 SGDID:S000002903, Chr IV from 1441142-1438107, reverse complement, Verified ORF, "Cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase" [yeast-contam.fasta] VPS3 SGDID:S000002903, Chr IV from 1441143-1438108, reverse complement, Verified ORF, "Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, an [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Vps3p: Vacuolar sorting protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] VPS3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase; Vps3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Vacuolar protein sorting-associated protein 3 (Vacuolar protein-targeting protein 17) [nr-271106-contam.fasta] VPS3 SGDID:S000002903, Chr IV from 1441143-1438108, reverse complement, Verified ORF, "Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Vacuolar protein sorting-associated protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=VPS3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] VPS3 SGDID:S000002903, Chr IV from 1441150-1438115, Genome Release 64-1-1, reverse complement, Verified ORF, "Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase" [yeast-030211-contam.fasta] VPS3 SGDID:S000002903, Chr IV from 1441150-1438115, Genome Release 64-2-1, reverse complement, Verified ORF, "Component of CORVET membrane tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase" [20141208_orf_trans.fasta] YDR495C SGDID:S000002903, Chr IV from 1441150-1438115, Genome Release 64-1-1, reverse complement, Verified ORF, "Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1011 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [TAS]
Biological Process:	vacuole inheritance [TAS] telomere maintenance [IMP] vacuolar acidification [IMP] protein targeting to vacuole [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVKKKTNNDK GKEVKENEGK LDIDSESSPH ERENDKKKTE DDSLRATESE ETNTHNANPN  60
   61 ETVRADKFSQ EESRPIEDSP HTDKNTAQES CQPSSAEDNV INTDITSLNE KTSTNDEQEK 120
  121 GLPLKISEGP FTISTLLDNV PSDLIYTCCE AYENHIFLGT TTGDLLHYFE LERGNYMLVS 180
  181 QTKFDAESNS KIDKILLLPK VEGALILCDN ELVLFILPEF APRPNTTRLK GISDVVICNF 240
  241 SRSSKAYRIY AFHAEGVRLL KISADSLVLT KAFNFKLIDK ACAHEETLMV SKLNSYELIN 300
  301 LKSSQVIPLF RISETDEDLE PIITSFNEQS EFLVCSGGGS YDSGAMALVV NHHGDIIKGT 360
  361 IVLKNYPRNV IVEFPYIIAE SAFQSVDIYS ALPSEKSQLL QSITTSGSDL KISKSDNVFT 420
  421 NTNNSEEFKE KIFNKLRLEP LTHSDNKFRI ERERAFVEES YEEKTSLIVY NNLGIHLLVP 480
  481 TPMVLRFTSC EESEIDNIED QLKKLAKKDL TKFEHIEAKY LMSLLLFLMT LHYDHIEDEV 540
  541 MKKWCDFSDK VDIRILFYMF GWKVYSEIWC FHGLINIVER LKSLKLTNKC ENILKMLLMM 600
  601 KNELKKKNKT GLLTNDFDDI MKTIDITLFK LRLEKKETIT VDMFERESYD EIIREINLHD 660
  661 DKLPRIELLI EIYKEKGEYL KALNLLREAG DYISLVSFIE ENLKKLPEDY IKERIADDLL 720
  721 LTLKQGDENT EECAIKKVLK ILDMACINKN DFLNKIPAEE TSLKVSFIEQ LGVQNSNDSK 780
  781 FLFNYYLAKL REIINQSNIW SILGDFIKEY KDDFAYDKTD ITNFIHIKLK HSLQCENFSK 840
  841 YYEKCENLKS ENEKDDEFIN FTFDEISKID KEHILTLLFF PNELTNWVSS EELLKIYLSF 900
  901 NDFRSVEKYI GKQNLVAVMK QYLDISSLNY SVELVTNLLQ RNFELLDDTD IQLKILETIP 960
  961 SVFPVQTISE LLLKVLIKYQ EKKEESNLRK CLLKNQISIS DELSRNFDSQ G

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		PLB3 RPS10B SCM3 SUP35 SUP45 TRM12 VPS3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	LIA1	VPS3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	VPS3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..98]	deduced	N/A	No confident structure predictions are available.
2	View Details	[99..726]	MSA	15.038994	View MSA. No confident structure predictions are available.
3	View Details	[727..1011]	deduced	N/A	No confident structure predictions are available.
4	View Details	[407..491]	deduced	N/A	No confident structure predictions are available.
5	View Details	[492..1011]	FFAS03	0.962	Clathrin heavy chain proximal leg segment

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
protein transporter activity	2.99345877012947	bayes_pls_golite062009
binding	2.33303322187387	bayes_pls_golite062009
transporter activity	1.85346023606579	bayes_pls_golite062009
substrate-specific transporter activity	1.51565516399356	bayes_pls_golite062009
protein binding	1.5114863464349	bayes_pls_golite062009
protein transmembrane transporter activity	1.12112006227201	bayes_pls_golite062009
transmembrane transporter activity	0.710528717985301	bayes_pls_golite062009
proton-transporting ATPase activity, rotational mechanism	0.68860343348996	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.39311980867126	bayes_pls_golite062009
signal sequence binding	0.279693431859311	bayes_pls_golite062009
nuclear localization sequence binding	0.241630311762028	bayes_pls_golite062009
nucleic acid binding	0.170608604167104	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.109435859778099	bayes_pls_golite062009
pyrophosphatase activity	0.0728493557966827	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.0641324729536009	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.0615190129942244	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2