Protein Overview: HIS4

General Information:
Name(s) found:	HIS4 / YCL030C [SGD]
Description(s) found: Found 23 descriptions. SHOW ALL	Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis [SGD]
Organism:	Saccharomyces cerevisiae
Length:	799 amino acids
Gene Ontology:
Cellular Component:	intracellular [TAS]
Biological Process:	histidine biosynthetic process [TAS]
Molecular Function:	phosphoribosyl-ATP diphosphatase activity [TAS] phosphoribosyl-AMP cyclohydrolase activity [TAS] histidinol dehydrogenase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVLPILPLID DLASWNSKKE YVSLVGQVLL DGSSLSNEEI LQFSKEEEVP LVALSLPSGK  60
   61 FSDDEIIAFL NNGVSSLFIA SQDAKTAEHL VEQLNVPKER VVVEENGVFS NQFMVKQKFS 120
  121 QDKIVSIKKL SKDMLTKEVL GEVRTDRPDG LYTTLVVDQY ERCLGLVYSS KKSIAKAIDL 180
  181 GRGVYYSRSR NEIWIKGETS GNGQKLLQIS TDCDSDALKF IVEQENVGFC HLETMSCFGE 240
  241 FKHGLVGLES LLKQRLQDAP EESYTRRLFN DSALLDAKIK EEAEELTEAK GKKELSWEAA 300
  301 DLFYFALAKL VANDVSLKDV ENNLNMKHLK VTRRKGDAKP KFVGQPKAEE EKLTGPIHLD 360
  361 VVKASDKVGV QKALSRPIQK TSEIMHLVNP IIENVRDKGN SALLEYTEKF DGVKLSNPVL 420
  421 NAPFPEEYFE GLTEEMKEAL DLSIENVRKF HAAQLPTETL EVETQPGVLC SRFPRPIEKV 480
  481 GLYIPGGTAI LPSTALMLGV PAQVAQCKEI VFASPPRKSD GKVSPEVVYV AEKVGASKIV 540
  541 LAGGAQAVAA MAYGTETIPK VDKILGPGNQ FVTAAKMYVQ NDTQALCSID MPAGPSEVLV 600
  601 IADEDADVDF VASDLLSQAE HGIDSQVILV GVNLSEKKIQ EIQDAVHNQA LQLPRVDIVR 660
  661 KCIAHSTIVL CDGYEEALEM SNQYAPEHLI LQIANANDYV KLVDNAGSVF VGAYTPESCG 720
  721 DYSSGTNHTL PTYGYARQYS GANTATFQKF ITAQNITPEG LENIGRAVMC VAKKEGLDGH 780
  781 RNAVKIRMSK LGLIPKDFQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ALA1 ARO1 DUG1 GDH1 HIS4 HIS6 HRD1 MET6 OYE2 RAD14 SCP160 SPE3 SPE4 TIM11 URA6 YLF2 YRB2
View Details	Gavin AC, et al. (2002)		DHH1 HIS4 PDC1 SAM1 TUB3
View Details	Ho Y, et al. (2002)		APC2 ARF2 FMP48 HIS4

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NOP9	No Comments	Hazbun TR, et al. (2003)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	HIS4	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
phosphoribosyl-AMP cyclohydrolase activity	4.07350794147607	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines	1.83474502448328	bayes_pls_golite062009
catalytic activity	1.02941336036162	bayes_pls_golite062009
cyclohydrolase activity	0.335226474674779	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	0.0625072987078716	bayes_pls_golite062009

Term	Confidence	Notes
phosphoribosyl-ATP diphosphatase activity	3.62350723585237	bayes_pls_golite062009
phosphoribosyl-AMP cyclohydrolase activity	2.54150905806868	bayes_pls_golite062009
catalytic activity	0.634427093790328	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines	0.324592130011053	bayes_pls_golite062009

Term	Confidence	Notes
catalytic activity	2.80804701752928	bayes_pls_golite062009
histidinol dehydrogenase activity	2.32012109012499	bayes_pls_golite062009
oxidoreductase activity	2.31275077585879	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors	2.24875477727877	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor	2.22720948878196	bayes_pls_golite062009

No functions predicted.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..55]	MSA	3.009987	View MSA. No confident structure predictions are available.
2	View Details	[56..120]	deduced	N/A	Confident ab initio structure predictions are available.
3	View Details	[121..243]	Pfam	50.124939	Phosphoribosyl-AMP cyclohydrolase No confident structure predictions are available.
4	View Details	[244..344]	Pfam	39.744727	Phosphoribosyl-ATP pyrophosphohydrolase No confident structure predictions are available.
5	View Details	[345..378] [593..799]	PSI-BLAST	1145.9897	L-histidinol dehydrogenase HisD
6	View Details	[379..592]	PSI-BLAST	1145.9897	L-histidinol dehydrogenase HisD

Protein Overview: HIS4

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: