YRC Public Data Repository - Protein Structure Prediction

Protein:	HIS4
Organism:	Saccharomyces cerevisiae
Length:	799 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for HIS4.

Description	E-value	Query Range	Subject Range
HIS4 - Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrol... HIS2_YEAST - Histidine biosynthesis trifunctional protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	0.0	[1..799]	[1..799]
gi\|1076975 - pir\|\|S53349 phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) / phosphoribosyl-ATP diphosphatase (EC 3... HIS2_SACBA - Histidine biosynthesis trifunctional protein OS=Saccharomyces bayanus GN=HIS4 PE=3 SV=1	0.0	[1..799]	[1..799]
gi\|4185813 - gi\|4185813\|gb\|AAD09196.1\| HMG1-SUC2-HIS4C protein [Shuttle vector pCS4-14]	0.0	[33..799]	[858..1624]
HIS2_KLULA - Histidine biosynthesis trifunctional protein OS=Kluyveromyces lactis GN=HIS4 PE=3 SV=2 HIS2_KLULA - Histidine biosynthesis trifunctional protein OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / ...	0.0	[2..799]	[1..795]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[1..794]	[1..844]
gi\|2623127 - gi\|2623127\|gb\|AAC53724.1\| histidinol dehydrogenase [Expression vector pIB4] gi\|2623118 - gi\|2623118\|gb\|AAC53718.1\| histidinol dehydrogenase [Expression vector pIB1] gi\|2623121 - gi\|2623121\|gb\|AAC53720.1\| histidinol dehydrogenase [Expression vector pIB2] gi\|2623124 - gi\|2623124\|gb\|AAC53722.1\| histidinol dehydrogenase [Expression vector pIB3]	0.0	[1..794]	[1..843]
gi\|537458 - gi\|537458\|gb\|AAA66982.1\| histidinol dehydrogenase gi\|537455 - gi\|537455\|gb\|AAA66980.1\| histidinol dehydrogenase gi\|537487 - gi\|537487\|gb\|AAA67000.1\| histidinol dehydrogenase gi\|537482 - gi\|537482\|gb\|AAA66998.1\| histidinol dehydrogenase HIS2_PICPA - Histidine biosynthesis trifunctional protein OS=Komagataella pastoris GN=HIS4 PE=3 SV=1 gi\|537479 - gi\|537479\|gb\|AAA66996.1\| histidinol dehydrogenase gi\|537476 - gi\|537476\|gb\|AAA66994.1\| histidinol dehydrogenase gi\|537473 - gi\|537473\|gb\|AAA66991.1\| histidinol dehydrogenase gi\|537470 - gi\|537470\|gb\|AAA66990.1\| histidinol dehydrogenase gi\|537467 - gi\|537467\|gb\|AAA66988.1\| histidinol dehydrogenase gi\|537464 - gi\|537464\|gb\|AAA66986.1\| histidinol dehydrogenase gi\|537461 - gi\|537461\|gb\|AAA66984.1\| histidinol dehydrogenase	0.0	[1..794]	[1..842]
gi\|447739 - gi\|447739\|prf\|\|1915304A nylB upstream ORF	0.0	[1..794]	[1..842]

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Predicted Domain #1

Region A:
Residues: [1-55]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVLPILPLID DLASWNSKKE YVSLVGQVLL DGSSLSNEEI LQFSKEEEVP LVALS

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [56-120]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LPSGKFSDDE IIAFLNNGVS SLFIASQDAK TAEHLVEQLN VPKERVVVEE NGVFSNQFMV  60
   61 KQKFS

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

		MCM Score	SCOP Match	SCOP Description
View	Download	0.944	d.68.5	C-terminal domain of ProRS

Predicted Domain #3

Region A:
Residues: [121-243]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QDKIVSIKKL SKDMLTKEVL GEVRTDRPDG LYTTLVVDQY ERCLGLVYSS KKSIAKAIDL  60
   61 GRGVYYSRSR NEIWIKGETS GNGQKLLQIS TDCDSDALKF IVEQENVGFC HLETMSCFGE 120
  121 FKH

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	50.124939
Match:	PF01502
Description:	Phosphoribosyl-AMP cyclohydrolase

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
phosphoribosyl-AMP cyclohydrolase activity	4.07350794147607	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines	1.83474502448328	bayes_pls_golite062009
catalytic activity	1.02941336036162	bayes_pls_golite062009
cyclohydrolase activity	0.335226474674779	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	0.0625072987078716	bayes_pls_golite062009

Predicted Domain #4

Region A:
Residues: [244-344]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GLVGLESLLK QRLQDAPEES YTRRLFNDSA LLDAKIKEEA EELTEAKGKK ELSWEAADLF  60
   61 YFALAKLVAN DVSLKDVENN LNMKHLKVTR RKGDAKPKFV G

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	39.744727
Match:	PF01503
Description:	Phosphoribosyl-ATP pyrophosphohydrolase

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
phosphoribosyl-ATP diphosphatase activity	3.62350723585237	bayes_pls_golite062009
phosphoribosyl-AMP cyclohydrolase activity	2.54150905806868	bayes_pls_golite062009
catalytic activity	0.634427093790328	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines	0.324592130011053	bayes_pls_golite062009

Predicted Domain #5

Region A:
Residues: [345-378]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QPKAEEEKLT GPIHLDVVKA SDKVGVQKAL SRPI

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [593-799]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 AGPSEVLVIA DEDADVDFVA SDLLSQAEHG IDSQVILVGV NLSEKKIQEI QDAVHNQALQ  60
   61 LPRVDIVRKC IAHSTIVLCD GYEEALEMSN QYAPEHLILQ IANANDYVKL VDNAGSVFVG 120
  121 AYTPESCGDY SSGTNHTLPT YGYARQYSGA NTATFQKFIT AQNITPEGLE NIGRAVMCVA 180
  181 KKEGLDGHRN AVKIRMSKLG LIPKDFQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1145.9897
Match:	1k75A_
Description:	L-histidinol dehydrogenase HisD
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
catalytic activity	2.80804701752928	bayes_pls_golite062009
histidinol dehydrogenase activity	2.32012109012499	bayes_pls_golite062009
oxidoreductase activity	2.31275077585879	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors	2.24875477727877	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor	2.22720948878196	bayes_pls_golite062009

Predicted Domain #6

Region A:
Residues: [379-592]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QKTSEIMHLV NPIIENVRDK GNSALLEYTE KFDGVKLSNP VLNAPFPEEY FEGLTEEMKE  60
   61 ALDLSIENVR KFHAAQLPTE TLEVETQPGV LCSRFPRPIE KVGLYIPGGT AILPSTALML 120
  121 GVPAQVAQCK EIVFASPPRK SDGKVSPEVV YVAEKVGASK IVLAGGAQAV AAMAYGTETI 180
  181 PKVDKILGPG NQFVTAAKMY VQNDTQALCS IDMP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1145.9897
Match:	1k75A_
Description:	L-histidinol dehydrogenase HisD
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions:

Predicted functions:

Predicted functions: