YRC Public Data Repository - Protein Structure Prediction

Protein:	HIS4
Organism:	Saccharomyces cerevisiae
Length:	799 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for HIS4.

Description	E-value	Query Range	Subject Range
HIS4 - Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrol... HIS2_YEAST - Histidine biosynthesis trifunctional protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	0.0	[1..799]	[1..799]
gi\|1076975 - pir\|\|S53349 phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) / phosphoribosyl-ATP diphosphatase (EC 3... HIS2_SACBA - Histidine biosynthesis trifunctional protein OS=Saccharomyces bayanus GN=HIS4 PE=3 SV=1	0.0	[1..799]	[1..799]
gi\|4185813 - gi\|4185813\|gb\|AAD09196.1\| HMG1-SUC2-HIS4C protein [Shuttle vector pCS4-14]	0.0	[33..799]	[858..1624]
HIS2_KLULA - Histidine biosynthesis trifunctional protein OS=Kluyveromyces lactis GN=HIS4 PE=3 SV=2 HIS2_KLULA - Histidine biosynthesis trifunctional protein OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / ...	0.0	[2..799]	[1..795]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[1..794]	[1..844]
gi\|2623127 - gi\|2623127\|gb\|AAC53724.1\| histidinol dehydrogenase [Expression vector pIB4] gi\|2623118 - gi\|2623118\|gb\|AAC53718.1\| histidinol dehydrogenase [Expression vector pIB1] gi\|2623121 - gi\|2623121\|gb\|AAC53720.1\| histidinol dehydrogenase [Expression vector pIB2] gi\|2623124 - gi\|2623124\|gb\|AAC53722.1\| histidinol dehydrogenase [Expression vector pIB3]	0.0	[1..794]	[1..843]
gi\|537458 - gi\|537458\|gb\|AAA66982.1\| histidinol dehydrogenase gi\|537455 - gi\|537455\|gb\|AAA66980.1\| histidinol dehydrogenase gi\|537487 - gi\|537487\|gb\|AAA67000.1\| histidinol dehydrogenase gi\|537482 - gi\|537482\|gb\|AAA66998.1\| histidinol dehydrogenase HIS2_PICPA - Histidine biosynthesis trifunctional protein OS=Komagataella pastoris GN=HIS4 PE=3 SV=1 gi\|537479 - gi\|537479\|gb\|AAA66996.1\| histidinol dehydrogenase gi\|537476 - gi\|537476\|gb\|AAA66994.1\| histidinol dehydrogenase gi\|537473 - gi\|537473\|gb\|AAA66991.1\| histidinol dehydrogenase gi\|537470 - gi\|537470\|gb\|AAA66990.1\| histidinol dehydrogenase gi\|537467 - gi\|537467\|gb\|AAA66988.1\| histidinol dehydrogenase gi\|537464 - gi\|537464\|gb\|AAA66986.1\| histidinol dehydrogenase gi\|537461 - gi\|537461\|gb\|AAA66984.1\| histidinol dehydrogenase	0.0	[1..794]	[1..842]
gi\|447739 - gi\|447739\|prf\|\|1915304A nylB upstream ORF	0.0	[1..794]	[1..842]

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Predicted Domain #1

Region A:
Residues: [1-55]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVLPILPLID DLASWNSKKE YVSLVGQVLL DGSSLSNEEI LQFSKEEEVP LVALS

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

		MCM Score	GO Score	GO Term	SCOP Match	SCOP Description
View	Download	0.630	0.085	histidinol dehydrogenase activity	a.8.1	Bacterial immunoglobulin/albumin-binding domains
View	Download	0.577	0.033	histidinol dehydrogenase activity	a.5.1	DNA helicase RuvA subunit, C-terminal domain
View	Download	0.728	N/A	N/A	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.676	N/A	N/A	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.655	N/A	N/A	a.179.1	Replisome organizer (g39p helicase loader/inhibitor protein)
View	Download	0.624	N/A	N/A	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.603	N/A	N/A	a.52.1	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin
View	Download	0.587	N/A	N/A	a.28.3	Retrovirus capsid protein C-terminal domain
View	Download	0.557	N/A	N/A	a.44.1	Disulphide-bond formation facilitator (DSBA), insertion domain
View	Download	0.550	N/A	N/A	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.542	N/A	N/A	a.153.1	Nuclear receptor coactivator interlocking domain
View	Download	0.526	N/A	N/A	a.4.1	Homeodomain-like
View	Download	0.503	N/A	N/A	a.4.1	Homeodomain-like
View	Download	0.438	N/A	N/A	a.4.1	Homeodomain-like
View	Download	0.433	N/A	N/A	a.5.2	UBA-like
View	Download	0.422	N/A	N/A	a.4.1	Homeodomain-like
View	Download	0.404	N/A	N/A	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.400	N/A	N/A	a.35.1	lambda repressor-like DNA-binding domains
View	Download	0.392	N/A	N/A	a.64.1	Saposin
View	Download	0.387	N/A	N/A	a.65.1	Annexin
View	Download	0.385	N/A	N/A	a.64.1	Saposin
View	Download	0.370	N/A	N/A	a.4.1	Homeodomain-like
View	Download	0.361	N/A	N/A	a.17.1	p8-MTCP1
View	Download	0.326	N/A	N/A	a.4.1	Homeodomain-like
View	Download	0.318	N/A	N/A	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.312	N/A	N/A	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.292	N/A	N/A	d.15.7	Immunoglobulin-binding domains
View	Download	0.290	N/A	N/A	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.281	N/A	N/A	a.5.7	4-hydroxy-2-oxovalerate aldolase DmpG, communication domain
View	Download	0.254	N/A	N/A	a.4.1	Homeodomain-like
View	Download	0.253	N/A	N/A	a.4.1	Homeodomain-like
View	Download	0.234	N/A	N/A	a.4.1	Homeodomain-like
View	Download	0.212	N/A	N/A	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.209	N/A	N/A	a.5.6	Hypothetical protein MTH1615
View	Download	0.203	N/A	N/A	a.48.3	Conserved domain common to transcription factors TFIIS, elongin A, CRSP70
View	Download	0.201	N/A	N/A	a.58.1	Chemotaxis receptor methyltransferase CheR, N-terminal domain

Predicted Domain #2

Region A:
Residues: [56-120]

      1          11         21         31         41         51         
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    1 LPSGKFSDDE IIAFLNNGVS SLFIASQDAK TAEHLVEQLN VPKERVVVEE NGVFSNQFMV  60
   61 KQKFS

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

		MCM Score	SCOP Match	SCOP Description
View	Download	0.738	d.68.3	SirA-like
View	Download	0.806	d.74.2	C-terminal domain of arginine repressor
View	Download	0.706	d.68.7	R3H domain
View	Download	0.944	d.68.5	C-terminal domain of ProRS
View	Download	0.682	d.68.3	SirA-like
View	Download	0.675	d.95.2	Homing endonucleases
View	Download	0.637	d.74.2	C-terminal domain of arginine repressor
View	Download	0.631	d.68.5	C-terminal domain of ProRS
View	Download	0.533	d.68.4	YhbY-like
View	Download	0.514	b.71.1	Glycosyl hydrolase domain
View	Download	0.478	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.408	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.407	b.71.1	Glycosyl hydrolase domain
View	Download	0.372	d.15.7	Immunoglobulin-binding domains
View	Download	0.370	d.95.1	Glucose permease domain IIB
View	Download	0.367	b.122.1	PUA domain-like
View	Download	0.365	b.1.18	E set domains
View	Download	0.365	d.95.2	Homing endonucleases
View	Download	0.365	b.1.1	Immunoglobulin
View	Download	0.313	d.64.1	eIF1-like
View	Download	0.288	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.287	d.68.5	C-terminal domain of ProRS
View	Download	0.281	b.1.18	E set domains
View	Download	0.276	d.68.1	Translation initiation factor IF3, C-terminal domain
View	Download	0.268	d.79.3	L30e-like
View	Download	0.253	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.245	c.5.1	MurCD N-terminal domain
View	Download	0.234	b.71.1	Glycosyl hydrolase domain
View	Download	0.230	a.65.1	Annexin
View	Download	0.225	c.52.2	tRNA splicing endonuclease, C-terminal domain
View	Download	0.223	c.51.1	Anticodon-binding domain of Class II aaRS
View	Download	0.208	b.1.1	Immunoglobulin

Predicted Domain #3

Region A:
Residues: [121-243]

      1          11         21         31         41         51         
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    1 QDKIVSIKKL SKDMLTKEVL GEVRTDRPDG LYTTLVVDQY ERCLGLVYSS KKSIAKAIDL  60
   61 GRGVYYSRSR NEIWIKGETS GNGQKLLQIS TDCDSDALKF IVEQENVGFC HLETMSCFGE 120
  121 FKH

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	50.124939
Match:	PF01502
Description:	Phosphoribosyl-AMP cyclohydrolase

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
phosphoribosyl-AMP cyclohydrolase activity	4.07350794147607	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines	1.83474502448328	bayes_pls_golite062009
catalytic activity	1.02941336036162	bayes_pls_golite062009
cyclohydrolase activity	0.335226474674779	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	0.0625072987078716	bayes_pls_golite062009

Predicted Domain #4

Region A:
Residues: [244-344]

      1          11         21         31         41         51         
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    1 GLVGLESLLK QRLQDAPEES YTRRLFNDSA LLDAKIKEEA EELTEAKGKK ELSWEAADLF  60
   61 YFALAKLVAN DVSLKDVENN LNMKHLKVTR RKGDAKPKFV G

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	39.744727
Match:	PF01503
Description:	Phosphoribosyl-ATP pyrophosphohydrolase

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
phosphoribosyl-ATP diphosphatase activity	3.62350723585237	bayes_pls_golite062009
phosphoribosyl-AMP cyclohydrolase activity	2.54150905806868	bayes_pls_golite062009
catalytic activity	0.634427093790328	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines	0.324592130011053	bayes_pls_golite062009

Predicted Domain #5

Region A:
Residues: [345-378]

      1          11         21         31         41         51         
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    1 QPKAEEEKLT GPIHLDVVKA SDKVGVQKAL SRPI

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [593-799]

      1          11         21         31         41         51         
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    1 AGPSEVLVIA DEDADVDFVA SDLLSQAEHG IDSQVILVGV NLSEKKIQEI QDAVHNQALQ  60
   61 LPRVDIVRKC IAHSTIVLCD GYEEALEMSN QYAPEHLILQ IANANDYVKL VDNAGSVFVG 120
  121 AYTPESCGDY SSGTNHTLPT YGYARQYSGA NTATFQKFIT AQNITPEGLE NIGRAVMCVA 180
  181 KKEGLDGHRN AVKIRMSKLG LIPKDFQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1145.9897
Match:	1k75A_
Description:	L-histidinol dehydrogenase HisD
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
catalytic activity	2.80804701752928	bayes_pls_golite062009
histidinol dehydrogenase activity	2.32012109012499	bayes_pls_golite062009
oxidoreductase activity	2.31275077585879	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors	2.24875477727877	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor	2.22720948878196	bayes_pls_golite062009

Predicted Domain #6

Region A:
Residues: [379-592]

      1          11         21         31         41         51         
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    1 QKTSEIMHLV NPIIENVRDK GNSALLEYTE KFDGVKLSNP VLNAPFPEEY FEGLTEEMKE  60
   61 ALDLSIENVR KFHAAQLPTE TLEVETQPGV LCSRFPRPIE KVGLYIPGGT AILPSTALML 120
  121 GVPAQVAQCK EIVFASPPRK SDGKVSPEVV YVAEKVGASK IVLAGGAQAV AAMAYGTETI 180
  181 PKVDKILGPG NQFVTAAKMY VQNDTQALCS IDMP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1145.9897
Match:	1k75A_
Description:	L-histidinol dehydrogenase HisD
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions:

Predicted functions:

Predicted functions: