YRC Public Data Repository - Protein Structure Prediction

Protein:	TPK3
Organism:	Saccharomyces cerevisiae
Length:	398 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for TPK3.

Description	E-value	Query Range	Subject Range
TPK3 - cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients... KAPC_YEAST - cAMP-dependent protein kinase type 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TPK... gi\|151941752 - gi\|151941752\|gb\|EDN60113.1\| cAMP-dependent protein kinase catalytic subunit [Saccharomyces cerevisia...	0.0	[1..398]	[1..398]
gi\|183396893, gi... - gi\|183396893\|gb\|AAI65948.1\| Protein kinase C, epsilon [synthetic construct], gi\|117616658\|gb\|ABK4234... KPCE_MOUSE - Protein kinase C epsilon type OS=Mus musculus GN=Prkce PE=1 SV=1	0.0	[4..398]	[311..728]
PRKCE - protein kinase C, epsilon gi\|168279093 - gi\|168279093\|dbj\|BAG11426.1\| protein kinase C epsilon type [synthetic construct]	0.0	[4..398]	[311..728]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[4..398]	[311..727]
KPCE_RABIT - Protein kinase C epsilon type OS=Oryctolagus cuniculus GN=PRKCE PE=2 SV=1	0.0	[4..398]	[311..727]

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Predicted Domain #1

Region A:
Residues: [1-78]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MYVDPMNNNE IRKLSITAKT ETTPDNVGQD IPVNAHSVHE ECSSNTPVEI NGRNSGKLKE  60
   61 EASAGICLVK KPMLQYRD

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

		MCM Score	SCOP Match	SCOP Description
View	Download	0.380	d.169.1	C-type lectin-like
View	Download	0.332	a.61.1	Retroviral matrix proteins
View	Download	0.424	a.60.2	RuvA domain 2-like
View	Download	0.394	c.55.4	Translational machinery components
View	Download	0.414	d.75.1	tRNA splicing endonuclease EdnA, N-terminal domain
View	Download	0.305	a.60.2	RuvA domain 2-like
View	Download	0.297	a.35.1	lambda repressor-like DNA-binding domains
View	Download	0.292	a.144.1	PABC (PABP) domain
View	Download	0.291	d.88.1	SRF-like
View	Download	0.263	d.186.1	Head-to-tail joining protein W, gpW
View	Download	0.249	d.21.1	Diaminopimelate epimerase-like
View	Download	0.236	b.2.2	Carbohydrate-binding domain
View	Download	0.235	d.58.3	Protease propeptides/inhibitors
View	Download	0.208	b.36.1	PDZ domain-like

Predicted functions:

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
cyclic nucleotide-dependent protein kinase activity	4.97855712164511	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
cAMP-dependent protein kinase activity	4.66277812893755	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
protein tyrosine kinase activity	2.8153155526437	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
transmembrane receptor activity	2.59526003830791	bayes_pls_golite062009
transmembrane receptor protein kinase activity	2.54265774272269	bayes_pls_golite062009
signal transducer activity	2.40586892801446	bayes_pls_golite062009
molecular transducer activity	2.40586892801446	bayes_pls_golite062009
receptor activity	2.39311927684508	bayes_pls_golite062009
telethonin binding	2.27062712605521	bayes_pls_golite062009
tubulin binding	2.18641675163967	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase activity	2.16831387024372	bayes_pls_golite062009
cytoskeletal protein binding	2.12615078788254	bayes_pls_golite062009
receptor signaling protein activity	2.10379226653669	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
microtubule binding	1.88110801192607	bayes_pls_golite062009
actin binding	1.87817875565699	bayes_pls_golite062009
acetylcholine receptor activator activity	1.83545227594542	bayes_pls_golite062009
phosphoinositide 3-kinase binding	1.82515074380651	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.81249386965144	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
insulin receptor substrate binding	1.62657794525303	bayes_pls_golite062009
transcription regulator activity	1.56748951434625	bayes_pls_golite062009
JUN kinase kinase kinase activity	1.41172362253967	bayes_pls_golite062009
alpha-actinin binding	1.38861866085902	bayes_pls_golite062009
DNA binding	1.35333135345112	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.32254739984688	bayes_pls_golite062009
gamma-tubulin binding	1.28511591054866	bayes_pls_golite062009
kinase binding	1.26086833316556	bayes_pls_golite062009
ErbB-3 class receptor binding	1.25765701903393	bayes_pls_golite062009
protein binding	1.22565748038754	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
nucleic acid binding	1.16049201286103	bayes_pls_golite062009
transporter activity	1.11833481501707	bayes_pls_golite062009
transcription factor activity	1.10928254946881	bayes_pls_golite062009
protein kinase binding	1.06030596516506	bayes_pls_golite062009
ErbB-2 class receptor binding	1.02996109010965	bayes_pls_golite062009
transmembrane transporter activity	0.97575992765505	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.959360414036299	bayes_pls_golite062009
growth factor binding	0.86076618871384	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	0.835012681606991	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	0.835012681606991	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	0.832111648073981	bayes_pls_golite062009
MAP kinase activity	0.81943579965027	bayes_pls_golite062009
actin filament binding	0.794152600084	bayes_pls_golite062009
substrate-specific transporter activity	0.705905455086486	bayes_pls_golite062009
ligase activity	0.665445021164512	bayes_pls_golite062009
structural constituent of cytoskeleton	0.612883428421652	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
enzyme binding	0.46284745266176	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.450699332958421	bayes_pls_golite062009
protein kinase C activity	0.41587987561486	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
MAP kinase kinase activity	0.35402045013148	bayes_pls_golite062009
insulin receptor binding	0.266450215230107	bayes_pls_golite062009
protein complex binding	0.239339759110451	bayes_pls_golite062009
insulin receptor activity	0.237134032032089	bayes_pls_golite062009
SH2 domain binding	0.226760162354251	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase activity	0.20643152368029	bayes_pls_golite062009
transforming growth factor beta receptor activity	0.20643152368029	bayes_pls_golite062009
receptor binding	0.197402253061255	bayes_pls_golite062009
ion transmembrane transporter activity	0.148194101970458	bayes_pls_golite062009
peptide binding	0.118578378753757	bayes_pls_golite062009
transforming growth factor beta receptor activity, type I	0.0506751594607393	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [79-137]

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    1 TSGKYSLSDF QILRTLGTGS FGRVHLIRSN HNGRFYALKT LKKHTIVKLK QVEHTNDER

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [151-167]

      1          11         21         31         41         51         
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    1 MWGTFQDSQQ VFMVMDY

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [363-398]

      1          11         21         31         41         51         
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    1 IQQGQGDTSQ FDRYPEEEFN YGIQGEDPYM DLMKEF

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1860.0
Match:	1fotA_
Description:	cAMP-dependent PK, catalytic subunit
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [138-150]

      1          11         21         31         41         51         
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    1 RMLSIVSHPF IIR

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [168-362]

      1          11         21         31         41         51         
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    1 IEGGELFSLL RKSQRFPNPV AKFYAAEVCL ALEYLHSKDI IYRDLKPENI LLDKNGHIKI  60
   61 TDFGFAKYVP DVTYTLCGTP DYIAPEVVST KPYNKSVDWW SFGVLIYEML AGYTPFYNSN 120
  121 TMKTYENILN AELKFPPFFH PDAQDLLKKL ITRDLSERLG NLQNGSEDVK NHPWFNEVIW 180
  181 EKLLARYIET PYEPP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1860.0
Match:	1fotA_
Description:	cAMP-dependent PK, catalytic subunit
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: