Bait Protein: | CLB2 |
Organism: | Saccharomyces cerevisiae |
Publication: | McCusker D, Denison C, Anderson S, Egelhofer TA, Yates JR 3rd, Gygi SP, Kellogg DR. Cdk1 coordinates cell-surface growth with the cell cycle.Nat Cell Biol. 2007 May;9(5):506-15. |
Comments: | No comments. |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
CDC28 | Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with ... | 23 | 61 | 59.4% | 0.0793 | 34061 |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 35 | 90 | 58.1% | 0.0546 | 69470 |
[ |
gi|7463016|pir||... | lysyl endopeptidase (EC 3.4.21.50) - Lysobacter enzymogenes | 13 | 40 | 58.0% | 0.0576 | 27961 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 32 | 81 | 55.1% | 0.0489 | 69768 |
[ |
gi|2119236, gi|4... | sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... | 28 | 149 | 47.4% | 0.0928 | 61987 |
[ |
SSB1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 27 | 65 | 46.5% | 0.0411 | 66602 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 26 | 62 | 44.4% | 0.0392 | 66595 |
[ |
SPA1_STAAU, cont... | 24 | 79 | 43.5% | 0.0584 | 57320 | |
[ |
Contaminant_KERA... | 25 | 50 | 39.8% | 0.0301 | 65494 | |
[ |
CLB2 | B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2... | 23 | 128 | 38.9% | 0.101 | 56247 |
[ |
KRT10 | keratin 10 | 26 | 39 | 37.6% | 0.0255 | 59519 |
[ |
contaminant_SPA2... | 18 | 67 | 34.6% | 0.0511 | 55439 | |
[ |
KRT2 | keratin 2 | 22 | 23 | 32.9% | 0.0138 | 65865 |
[ |
RPL12B, RPL12A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... | 3 | 4 | 28.5% | 0.00939 | 17823 |
[ |
RPL12B, RPL12A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... | 3 | 4 | 28.5% | 0.00939 | 17823 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 2 | 2 | 26.4% | 0.00704 | 11050 |
[ |
SSC1 | Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... | 14 | 17 | 24.0% | 0.0101 | 70628 |
[ |
KRT5 | keratin 5 | 16 | 21 | 21.4% | 0.0138 | 62461 |
[ |
KRT17 | keratin 17 | 9 | 10 | 21.1% | 0.00899 | 47974 |
[ |
gi|34069 | gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] | 9 | 16 | 20.4% | 0.0174 | 39219 |
[ |
Contaminant_KERA... | 12 | 13 | 19.7% | 0.0107 | 51531 | |
[ |
SSA3 | ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotrans... | 14 | 37 | 19.3% | 0.0221 | 70547 |
[ |
PSP1 | Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) an... | 9 | 13 | 18.8% | 0.00599 | 95348 |
[ |
RPP0 | Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... | 5 | 7 | 18.6% | 0.00869 | 33717 |
[ |
contaminant_KERA... | 8 | 8 | 17.7% | 0.00661 | 50499 | |
[ |
contaminant_KERA... | isb|A33652|A33652|probable keratin 16, type I, pseudogene - human | 8 | 11 | 17.1% | 0.00901 | 50915 |
[ |
contaminant_KERA... | 8 | 15 | 15.5% | 0.0103 | 59822 | |
[ |
RPL25 | Primary rRNA-binding ribosomal protein component of the large (60S) ribosomal subunit, has similarit... | 2 | 3 | 15.5% | 0.00818 | 15758 |
[ |
SSA4 | Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational... | 12 | 31 | 14.3% | 0.0187 | 69651 |
[ |
NOT5 | Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcrip... | 4 | 5 | 13.4% | 0.00346 | 65855 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 6 | 6 | 11.0% | 0.00341 | 74468 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 6 | 6 | 11.0% | 0.00341 | 74468 |
[ |
RPL2A, RPL2B | Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E.... | 2 | 2 | 11.0% | 0.00305 | 27408 |
[ |
RPL2A, RPL2B | Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E.... | 2 | 2 | 11.0% | 0.00305 | 27408 |
[ |
INT-STD1, Contam... | 1 | 5 | 6 | 10.7% | 0.00383 | 69271 |
[ |
ECM10 | Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protei... | 5 | 6 | 9.8% | 0.00361 | 70085 |
[ |
KRT20 | keratin 20 | 3 | 4 | 9.7% | 0.00365 | 48487 |
[ |
gi|1143492, gi|8... | pir||A29821 dnaK-type molecular chaperone HSPA5 precursor - human, gi|386758|gb|AAA52614.1| GRP78 p... | 3 | 7 | 8.4% | 0.00415 | 72116 |
[ |
contaminant_GR78... | 3 | 7 | 8.4% | 0.00415 | 72379 | |
[ |
gi|109893 | pir||A37048 dnaK-type molecular chaperone grp78 precursor - mouse | 3 | 7 | 8.4% | 0.00414 | 72421 |
[ |
GRP78_RAT | 78 kDa glucose-regulated protein OS=Rattus norvegicus GN=Hspa5 PE=1 SV=1 | 3 | 7 | 8.4% | 0.00415 | 72347 |
[ |
CHC1 | Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport ... | 6 | 6 | 6.8% | 0.00141 | 187233 |
[ |
KRT76 | keratin 76 | 5 | 5 | 6.6% | 0.00304 | 65871 |
[ |
contaminant_NRL_... | owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... | 3 | 3 | 6.3% | 0.00272 | 46852 |
[ |
Contaminant_KERA... | 5 | 5 | 6.2% | 0.00363 | 57265 | |
[ |
KRT19 | keratin 19 | 3 | 3 | 6.2% | 0.00291 | 44106 |
[ |
PHO85 | Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to n... | 3 | 5 | 6.2% | 0.00635 | 34906 |
[ |
KRT3 | keratin 3 | 5 | 6 | 5.9% | 0.0037 | 64511 |
[ |
KRT15 | keratin 15 | 4 | 4 | 5.9% | 0.0034 | 49168 |
[ |
gi|105815 | pir||A34720 keratin 8, type II cytoskeletal - human | 5 | 6 | 5.8% | 0.00481 | 53748 |
[ |
ETR1 | 2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to... | 1 | 12 | 5.3% | 0.0122 | 42067 |
[ |
contaminant_KERA... | 3 | 3 | 5.1% | 0.00248 | 51203 | |
[ |
gi|34033, gi|71526 | pir||KRHU3 keratin 13, type I, cytoskeletal, long splice form - human, gi|34033|emb|CAA32786.1| ker... | 3 | 3 | 4.8% | 0.00254 | 49644 |
[ |
OPT2 | Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albi... | 2 | 2 | 4.2% | 8.83E-4 | 101261 |
[ |
contaminant_GR78... | 4 | 10 | 4.2% | 0.00582 | 73235 | |
[ |
RGA1 | GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of se... | 2 | 6 | 3.7% | 0.00231 | 112831 |
[ |
BUD2 | GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutan... | 3 | 4 | 3.6% | 0.0014 | 126663 |
[ |
PTP2 | Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated prote... | 2 | 3 | 3.6% | 0.00155 | 85868 |
[ |
GR78_MAIZE, cont... | 2 | 8 | 3.6% | 0.00664 | 51689 | |
[ |
contaminant_GR78... | 2 | 2 | 3.5% | 0.00114 | 74448 | |
[ |
GR78_SCHPO, cont... | 2 | 2 | 3.2% | 0.00117 | 73081 | |
[ |
CLB1 | B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2... | 1 | 10 | 3.0% | 0.00822 | 54871 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 2 | 2 | 2.4% | 0.00169 | 50033 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 2 | 2 | 2.4% | 0.00169 | 50033 |
[ |
YLR001C | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... | 2 | 3 | 2.2% | 0.00135 | 97618 |
[ |
SIP1 | Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar prote... | 2 | 2 | 2.2% | 8.98E-4 | 96259 |
[ |
FAS1 | Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty a... | 2 | 2 | 2.1% | 3.78E-4 | 228689 |
[ |
NUP192 | Essential structural subunit of the nuclear pore complex (NPC), localizes to the nuclear periphery o... | 2 | 2 | 1.7% | 4.6E-4 | 191534 |
[ |
RGD1 | GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytosk... | 2 | 2 | 1.7% | 0.00116 | 74619 |
[ |
FAS2 | Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty ... | 2 | 2 | 1.5% | 4.11E-4 | 206945 |