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Protein Overview: BUD4

General Information:
Name(s) found: BUD4 / YJR092W [SGD]
Description(s) found:
Found 12 descriptions.
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  • Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding; potential Cdc28p substrate [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1448 amino acids

    Gene Ontology:
    Cellular Component: cellular bud neck contractile ring [TASLink to PubMed article]
    Biological Process: axial cellular bud site selection [TASLink to PubMed article]
    cellular bud site selection [TASLink to PubMed article]
    Molecular Function: GTP binding [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MHDAESTVDS LLKEIDNEME QTKSNITQNG SEDTPHNWKL PLQEIGDDTM EMLVKHNTRS  60
   61 NATENSRGRS PSKMSTISNE SLNLGLLRVN SELEESPAAV HQERIKNSVA NGALGHANSP 120
  121 KVLNNLKNMA QDIDKLARDE EKPVKLSSSP LKFTLKSTQP LLSYPESPIH RSSIEIETNY 180
  181 DDEDEEEEDA YTCLTQSPQI LHSPSRIPIT NAVSINKLNL DFTLNPNESD KSLVSDTSVD 240
  241 STGRELDTKT IPELPFCMSS TPEMTPVDEK CNLPSKLLNT SNNSHSDSRS ANSLCGGFKH 300
  301 FNESSRVLIP AKIIQSLLMR MRLLKTMLCR DLQQNMEHID EAFDEKKVLD EGCSNEPVTF 360
  361 LGENDTRSIV YSNKGTNANV QEFSQEDSLA HSEPKFKDLN ATSDDVWNED KETDANISTS 420
  421 TKSEESYIAD YKVTRQEDWD TKKLHQESEH ANEQPAIIPQ KDSSEETFTE LNNESEFQRN 480
  481 FKDGEEYRIV QHEESLYGQR TKSPEENIIN GSEIGVDHGE AAEVNEPLAK TSAEEHDLSS 540
  541 SCEDQSVSEA RNKDRIEEKE VETKDENIET EKDESEYHKV EENEEPEHVP LLPPLPRWEE 600
  601 IQFNEPFIDE NDTSNDSIDL TRSMKPSDYI SIWHIQEEEI KSNSPESIAN SQFSQQSSIT 660
  661 TASTVDSKKD NGSTSFKFKP RIVSRSRIYN PKSRVSSLNY YDNEDYILSN SEWNALDPMR 720
  721 RNTLISKRIQ DNIRTQKGHA PLIRPSIMKL NGEDSGFQNH FLEVEQPQEH ENIPLSTHLS 780
  781 EQDITTNVGL DEQKLPTNTQ DEAEISIREI ESAGDITFNR GDLLSLSFDE ELGQDFANFL 840
  841 DALDHDSTSF NHGPDDSSSF QRDSSKKSFN SLWESSYELK PPPSIRKQPI APDVLQKLLE 900
  901 SDTKDDADLE KIREERITEP RTGLGIGMLK TPVKDVSIAL AASIKGYEAS FSDTDSRPEG 960
  961 MNNSDAITLN MFDDFEEDKM TPSTPVRSIS PIKRHVSSPF KVVKAGNKQE NNEINIKAEE1020
 1021 EIEPMTQQET DGLKQDIPPL LAQTKDNVEA KEETITQLEE PQDVEQEFPD MGTLYLSIKA1080
 1081 ISTLALYGTK SHRATYAIVF DNGENVVQTP WESLPYDGNI RINKEFELPI DFKGKAETSS1140
 1141 ASSERDSYKK CVITLKCKYE KPRHELVEIV DKVPVGKSFF GKTKYKFEKK YVQKKPKQDE1200
 1201 WDYLFAQDGS FARCEIEINE EFLKNVAFNT SHMHYNMINK WSRIADKIHG SKRLYELPRK1260
 1261 APHKVASLDV EACFLERTSA FEQFPKQFSL VNKIVSKYKL QQNIYKEGYL LQDGGDLKGK1320
 1321 IENRFFKLHG SQLSGYHEIS RKAKIDINLL KVTKVLRNED IQADNGGQRN FTDWVLFNEC1380
 1381 FQLVFDDGER ITFNAECSNE EKSDWYNKLQ EVVELNVFHQ PWVKKYCEKL AEEEKTRTTG1440
 1441 HNLKQDFN

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Krogan NJ, et al. (2006) BUD4 CDC13 CDC15 GIR2 PRS5 RBG2 YIL152W
    View Details Gavin AC, et al. (2006) BUD4 CDC10 CDC11 CDC12 CDC3 SHS1
    View Details Qiu et al. (2008) BUD3 BUD4 CDC11 CDC12 ELM1 PAN1 PFY1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen RPT3 BUD4 1 Unpublished Fields Lab Data
    View Screen TWF1 BUD4 1 Unpublished Fields Lab Data
    View Screen LOS1 BUD4 1 Unpublished Fields Lab Data
    View Screen UBC7 BUD4 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data BUD4 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..164] MSA 1.002942 View MSA. No confident structure predictions are available.
    2 View Details [165..760] deduced N/A No confident structure predictions are available.
    3 View Details [761..879] MSA 1.004984 View MSA. No confident structure predictions are available.
    4 View Details [880..1261] deduced N/A No confident structure predictions are available.
    5 View Details [1262..1448] Pfam 12.251812 PH domain No confident structure predictions are available.
    6 View Details [760..840] MSA 1.006984 View MSA. No confident structure predictions are available.
    7 View Details [841..1059] deduced N/A No confident structure predictions are available.
    8 View Details [1060..1156] deduced N/A No confident structure predictions are available.
    9 View Details [1157..1257] deduced N/A No confident structure predictions are available.
    10 View Details [1258..1448] FFAS03 1.68 No description for 2dhiA was found.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle