Protein Overview: Mcm3-PA

General Information:
Name(s) found:	Mcm3-PA / FBpp0070729 [FlyBase]
Description(s) found: SHOW ONLY BEST	gi\|7290573\|gb\|AAF46023.1\| minichromosome maintenance 3 [Drosophila melanogaster] [NCBI NR] gi\|4903292\|gb\|AAD32859.1\|AF124745_1 DNA replication factor MCM3 [Drosophila melanogaster] [NCBI NR] gi\|24639835\|ref\|NP_511048.2\| minichromosome maintenance 3 [Drosophila melanogaster] [NCBI NR] gi\|209418012\|gb\|ACI46544.1\| LP01948p [Drosophila melanogaster] [NCBI NR] DNA replication licensing factor Mcm3 OS=Drosophila melanogaster GN=Mcm3 PE=1 SV=1 [Swiss-Prot] The gene Minichromosome maintenance 3 is referred to in FlyBase by the symbol Dmel\Mcm3 (CG4206, FBgn0024332). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is X:5217881..5220771. It has the cytological map location 4F5. There is experimental evidence that it has the molecular function</span>: 3'-5' DNA helicase activity. Based on sequence similarity, it is predicted to be involved in the biological process</span>: pre-replicative complex assembly. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. [FlyBase] >CG4206-PA translation from_gene[CG4206 Mcm3 FBgn0024332] seq_release:3 mol_weight=90919 cds_boundaries:(X:5,064,611..5,067,204[+]) aa_length:819 transcript_info:[CG4206-RA seq_release:3] gene_info:[gene symbol:Mcm3 FBgn0024332 gene_boundaries:(X:5,064 [hmr_yeast5_DM3-1_pombe.fasta] (Q9XYU1) MCM3 protein [DrosMelano7227.FASTAC.txt] (AE003434) Mcm3 gene product [Drosophila melanogaster] [yeastflysequest.fasta] CG4206-PA [Drosophila melanogaster]gi\|24639835\|ref\|NP_511048.2\| CG4206-PA [Drosophila melanogaster]gi\|4903292\|gb\|AAD32859.1\| DNA replication factor MCM3 [Drosophila melanogaster] [nrdb_11152004_Chlamydomonas] type=protein; loc=X:join(5218030..5219388,5219452..5219685,5219760..5220626); ID=FBpp0070729; name=Mcm3-PA; parent=FBgn0024332,FBtr0070762; dbxref=FlyBase:FBpp0070729,FlyBase_Annotation_IDs:CG4206-PA,GB_protein:AAF46023.1,REFSEQ:NP_511048,GB_protein:AAF46 [fly-100309-contam.fasta] DNA replication factor MCM3 [Drosophila melanogaster] [nr-271106-contam.fasta] CG4206-PA [Drosophila melanogaster] [nr-271106-contam.fasta] Minichromosome maintenance 3 CG4206-PA [Drosophila melanogaster] [nr-271106-contam.fasta] type=protein; loc=X:join(5218030..5219388,5219452..5219685,5219760..5220626); ID=FBpp0070729; name=Mcm3-PA; parent=FBgn0024332,FBtr0070762; dbxref=FlyBase:FBpp0070729,FlyBase_Annotation_IDs:CG4206-PA,GB_protein:AAF46023.1,REFSEQ:NP_511048,GB_protein:AAF46023; MD5=a3bfa1beff62680ceb3d794302f4c030; length=819; release=r5.16; species=Dmel; [fly-contam-His-TEV-Tub4complex.fasta] type=protein; loc=X:join(5218030..5219388,5219452..5219685,5219760..5220626); ID=FBpp0070729; name=Mcm3-PA; parent=FBgn0024332,FBtr0070762; dbxref=FlyBase:FBpp0070729,FlyBase_Annotation_IDs:CG4206-PA,GB_protein:AAF46023.1,REFSEQ:NP_511048,GB_protein:AAF46023; MD5=a3bfa1beff62680ceb3d794302f4c030; length=819; release=r5.21; species=Dmel; [Other_drosophila_na_09-09-2009_reversed.fasta] type=protein; loc=X:join(5218030..5219388,5219452..5219685,5219760..5220626); ID=FBpp0070729; name=Mcm3-PA; parent=FBgn0024332,FBtr0070762; dbxref=FlyBase:FBpp0070729,FlyBase_Annotation_IDs:CG4206-PA,GB_protein:AAF46023.1,REFSEQ:NP_511048,GB_protein:AAF46023,FlyMine:FBpp0070729,modMine:FBpp0070729; MD5=a3bfa1beff62680ceb3d794302f4c030; length=819; release=r5.46; species=Dmel; [fly-062812-sfps-sandra-contam.fasta] type=protein; loc=X:join(5323997..5325355,5325419..5325652,5325727..5326593); ID=FBpp0070729; name=Mcm3-PA; parent=FBgn0024332,FBtr0070762; dbxref=FlyBase:FBpp0070729,FlyBase_Annotation_IDs:CG4206-PA,GB_protein:AAF46023.1,REFSEQ:NP_511048,GB_protein:AAF46023,FlyMine:FBpp0070729,modMine:FBpp0070729; MD5=a3bfa1beff62680ceb3d794302f4c030; length=819; release=r6.02; species=Dmel; [fly-2014-05-contam.fasta] type=protein; loc=X:join(5323997..5325355,5325419..5325652,5325727..5326593); ID=FBpp0070729; name=Mcm3-PA; parent=FBgn0024332,FBtr0070762; dbxref=FlyBase:FBpp0070729,FlyBase_Annotation_IDs:CG4206-PA,GB_protein:AAF46023.1,REFSEQ:NP_511048,GB_protein:AAF46023,FlyMine:FBpp0070729,modMine:FBpp0070729; MD5=a3bfa1beff62680ceb3d794302f4c030; length=819; release=r6.07; species=Dmel; [fly-08202015-contam.fasta] FBpp0070729 type=protein; loc=X:join(5323997..5325355,5325419..5325652,5325727..5326593); ID=FBpp0070729; name=Mcm3-PA; parent=FBgn0024332,FBtr0070762; dbxref=FlyBase:FBpp0070729,FlyBase_Annotation_IDs:CG4206-PA,GB_protein:AAF46023.1,REFSEQ:NP_511048,GB_protein:AAF46023,FlyMine:FBpp0070729,modMine:FBpp0070729; MD5=a3bfa1beff62680ceb3d794302f4c030; length=819; release=r6.09; species=Dmel; [dmel-all-translation-r6.09.fasta] [fly-122215-dmel-6-09-contam.fasta] type=protein; loc=X:join(5323997..5325355,5325419..5325652,5325727..5326593); ID=FBpp0070729; name=Mcm3-PA; parent=FBgn0284442,FBtr0070762; dbxref=FlyBase:FBpp0070729,FlyBase_Annotation_IDs:CG4206-PA,GB_protein:AAF46023.1,REFSEQ:NP_511048,GB_protein:AAF46023,UniProt/Swiss-Prot:Q9XYU1,FlyMine:FBpp0070729,modMine:FBpp0070729; MD5=a3bfa1beff62680ceb3d794302f4c030; length=819; release=r6.14; species=Dmel; [fly-dmel-jan2017-sandracontam.fasta]
Organism:	Drosophila melanogaster
Length:	819 amino acids
Gene Ontology:
Cellular Component:	pre-replicative complex [ISS][NAS]
Biological Process:	DNA replication initiation [IEA] DNA replication [IEA] pre-replicative complex assembly [ISS][NAS]
Molecular Function:	DNA binding [IEA] ATP binding [IEA] chromatin binding [ISS][NAS] 3'-5' DNA helicase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAHEGEQFIK DIQREYVDFL DDEEDQGIYA GHVKDMIAEK SKRLIVNVND LKRKNPQRAL  60
   61 GLLSNAADEQ LAFGRALKEY ASTVDPGYAK MHEDLFVGFE GCFGNRHVTP RSLTSIYLGN 120
  121 MVCVEGIVTK VSLIRPKVVR SVHYCPNTRK VMERKYTDLT SFEAVPSGAA YPTKDEDGNL 180
  181 LETEYGLSVY KDHQTLTIQE MPEKAPAGQL PRSVDIVCDD DLVDRCKPGD RVQIVGSYRC 240
  241 LPGKRGGYTS GTFRTVLLAN NISLLSKESN LDISREDIML CKKLAKNNDI FELLSKSLAP 300
  301 SIHGHAYVKQ AILCLLLGGV EKILPNGTRL RGDINVLLIG DPSVAKSQLL RYVLNTAPRA 360
  361 IPTTGRGSSG VGLTAAVTTD QETGERRLEA GAMVLADRGV VCIDEFDKMS DIDRTAIHEV 420
  421 MEQGRVTISK AGIHASLNAR CSVLAAANPV YGRYDQYKTP MENIGLQDSL LSRFDLLFVM 480
  481 LDVIDSDVDQ MISDHVVRMH RYRNPKEADG EPLSMGSSYA DSLSFVSSSE EKKDTEVYEK 540
  541 YDALLHGKSR QRHEKILSVE FMRKYIHIAK CMKPKLGEQA CEAIANEYSR LRSQEAVETD 600
  601 VARTQPITAR TLETLIRLST AHARARMSKS VTIDDAHAAI ELVQFAYFKK VLDKDRPSKR 660
  661 RRNSGSDAED DNGEASSQRS PSRRSKRTRT ATVGADSDEE DIEPPQPDAG DLTRRETRRS 720
  721 LPARSVAMLM ASPSSEEQSV ATSTTEPAII SDARLGEFKN NLQRLFREAR EQSLALARIT 780
  781 TAINVGSQEP FTAGEIEAAV HRMTEDNQIM VADDIVFLI

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..226]	PSI-BLAST	44.0	DNA replication initiator (cdc21/cdc54) N-terminal domain
2	View Details	[227..304]	PSI-BLAST	48.30103	Crystal structure of sigm54 activator (AAA+ ATPase) in the inactive state
3	View Details	[305..737]	PSI-BLAST	43.221849	EDTA treated
4	View Details	[738..819]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
telomeric DNA binding	4.81350293592083	bayes_pls_golite062009
telomerase inhibitor activity	4.48082903165152	bayes_pls_golite062009
single-stranded telomeric DNA binding	4.34033743483519	bayes_pls_golite062009
nucleic acid binding	4.29626483284963	bayes_pls_golite062009
DNA binding	3.71771169141041	bayes_pls_golite062009
structural constituent of ribosome	3.51998515668903	bayes_pls_golite062009
structure-specific DNA binding	3.48139490984491	bayes_pls_golite062009
single-stranded DNA binding	3.43027351695641	bayes_pls_golite062009
structural molecule activity	3.29857127235306	bayes_pls_golite062009
binding	2.83465364476231	bayes_pls_golite062009
transcription regulator activity	2.10110937001387	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	2.04350689362549	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	2.04350689362549	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	2.04218282227834	bayes_pls_golite062009
sequence-specific DNA binding	1.9581702424972	bayes_pls_golite062009
DNA replication origin binding	1.84819558112187	bayes_pls_golite062009
translation regulator activity	1.46320772716954	bayes_pls_golite062009
translation factor activity, nucleic acid binding	1.40718318783582	bayes_pls_golite062009
transcription repressor activity	1.30343463892105	bayes_pls_golite062009
translation initiation factor activity	1.27539679024898	bayes_pls_golite062009
transcription activator activity	1.24141936405635	bayes_pls_golite062009
protein binding	1.20309946451799	bayes_pls_golite062009
RNA polymerase activity	1.19766815287926	bayes_pls_golite062009
DNA-directed RNA polymerase activity	1.19766815287926	bayes_pls_golite062009
ribonuclease activity	1.19236946227657	bayes_pls_golite062009
transcription factor activity	1.14242112812892	bayes_pls_golite062009
DNA helicase activity	1.12203697857261	bayes_pls_golite062009
ligase activity	1.06979791212194	bayes_pls_golite062009
DNA-dependent ATPase activity	0.638831894247026	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.635129001787169	bayes_pls_golite062009
nuclease activity	0.57381531077345	bayes_pls_golite062009
aspartate-tRNA ligase activity	0.504302440847948	bayes_pls_golite062009
nucleotidyltransferase activity	0.325774164899685	bayes_pls_golite062009
double-stranded DNA binding	0.314881439089948	bayes_pls_golite062009
exonuclease activity	0.28313884527829	bayes_pls_golite062009
transferase activity	0.249054945579328	bayes_pls_golite062009
RNA binding	0.18502033889261	bayes_pls_golite062009
exoribonuclease activity, producing 5'-phosphomonoesters	0.125256208482441	bayes_pls_golite062009
exoribonuclease activity	0.109774918833391	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.0954216620147701	bayes_pls_golite062009
DEAD/H-box RNA helicase binding	0.0627405398613412	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.0179535526984695	bayes_pls_golite062009
helicase activity	0.0121541775159524	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
DNA clamp loader activity	4.91429252297969	bayes_pls_golite062009
protein-DNA loading ATPase activity	4.80695929076807	bayes_pls_golite062009
DNA-dependent ATPase activity	2.72462789867829	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	2.66107861968804	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
nucleic acid binding	2.51683451033161	bayes_pls_golite062009
DNA helicase activity	2.2817164193758	bayes_pls_golite062009
pyrophosphatase activity	2.13284212711072	bayes_pls_golite062009
DNA binding	2.12258000781149	bayes_pls_golite062009
transcription regulator activity	2.09345198747025	bayes_pls_golite062009
telomeric DNA binding	2.0142766759353	bayes_pls_golite062009
ATP-dependent DNA helicase activity	1.88455339138556	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
DNA replication origin binding	1.62491387774159	bayes_pls_golite062009
ribonucleotide binding	1.53142082854758	bayes_pls_golite062009
purine nucleotide binding	1.52938239070386	bayes_pls_golite062009
purine ribonucleotide binding	1.52887308780164	bayes_pls_golite062009
nucleotide binding	1.52746968836881	bayes_pls_golite062009
structural constituent of ribosome	1.47858140861683	bayes_pls_golite062009
microtubule motor activity	1.35938517416356	bayes_pls_golite062009
ATP binding	1.23942044024113	bayes_pls_golite062009
protein binding	1.22129154759482	bayes_pls_golite062009
adenyl ribonucleotide binding	1.21044170258803	bayes_pls_golite062009
structure-specific DNA binding	1.20315703345493	bayes_pls_golite062009
adenyl nucleotide binding	1.19481945569892	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
motor activity	1.14667913749579	bayes_pls_golite062009
kinase activity	1.11120928120082	bayes_pls_golite062009
histone acetyltransferase activity	1.05990253628867	bayes_pls_golite062009
lysine N-acetyltransferase activity	1.05990253628867	bayes_pls_golite062009
double-stranded DNA binding	1.05408274676326	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.971476020912368	bayes_pls_golite062009
DNA polymerase activity	0.928091843881235	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.908046791062776	bayes_pls_golite062009
transcription activator activity	0.889929694023098	bayes_pls_golite062009
transcription factor activity	0.887108427603557	bayes_pls_golite062009
structural molecule activity	0.86514677273731	bayes_pls_golite062009
transcription repressor activity	0.862685397469217	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.8469248456592	bayes_pls_golite062009
single-stranded telomeric DNA binding	0.700780808446722	bayes_pls_golite062009
sequence-specific DNA binding	0.543891899378373	bayes_pls_golite062009
cytoskeletal protein binding	0.500462538667228	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.421921195209539	bayes_pls_golite062009
ligase activity	0.417681194526243	bayes_pls_golite062009
single-stranded DNA binding	0.406600732518248	bayes_pls_golite062009
protein kinase activity	0.351308564667977	bayes_pls_golite062009
translation regulator activity	0.29096092258568	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.274994961137851	bayes_pls_golite062009
RNA helicase activity	0.18426050934025	bayes_pls_golite062009
transferase activity	0.175269600441384	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	0.156278588350022	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.147579541298736	bayes_pls_golite062009
RNA-dependent ATPase activity	0.14606996093053	bayes_pls_golite062009
mismatched DNA binding	0.13366911641814	bayes_pls_golite062009
ATP-dependent RNA helicase activity	0.13050483816765	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.113504049290503	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.111293884322157	bayes_pls_golite062009
3'-5' DNA helicase activity	0.0769112340058804	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	0.0223627159838333	bayes_pls_golite062009
actin binding	0.00482938540552702	bayes_pls_golite062009

No functions predicted.

Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Protein Overview: Mcm3-PA

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: