Protein Overview: COT1

General Information:
Name(s) found:	COT1 / YOR316C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|940847\|emb\|CAA62171.1\| orf 06131 [Saccharomyces cerevisiae] [NCBI NR] gi\|6324892\|ref\|NP_014961.1\| Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; Cot1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S58327 cobalt accumulation protein COT1 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1706005\|sp\|P32798.2\|COT1_YEAST RecName: Full=Cobalt uptake protein COT1 [NCBI NR] gi\|1420694\|emb\|CAA99636.1\| COT1 [Saccharomyces cerevisiae] [NCBI NR] Cobalt uptake protein COT1 OS=Saccharomyces cerevisiae GN=COT1 PE=1 SV=2 [Swiss-Prot] COT1 Vacuolar zinc (and possibly other metals) transporter [mips] Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium [SGD] (P32798) Cobalt uptake protein COT1 [4932.FASTACCERV.txt] Chr XV from 907548-906229, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 906228-907547, reverse complement [yeastorf3.fasta] Chr XV from 906229-907548, reverse complement [yeastorf4.fasta] COT1, Chr XV from 906228-907547, reverse complement [yeast_orfs2] COT1 SGDID:S0005843, Chr XV from 907550-906231, reverse complement, Verified ORF [yeastflysequest.fasta] Cot1p [Saccharomyces cerevisiae]gi\|1420694\|emb\|CAA99636.1\| COT1 [Saccharomyces cerevisiae]gi\|940847\|emb\|CAA62171.1\| orf 06131 [Saccharomyces cerevisiae]gi\|1706005\|sp\|P32798\|COT1_YEAST Cobalt uptake protein COT1gi\|2131216\|pir\|\|S58327 cobalt accumulatio [nrdb_11152004_Chlamydomonas] COT1 SGDID:S000005843, Chr XV from 907550-906231, reverse complement, Verified ORF, "Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] COT1 SGDID:S000005843, Chr XV from 907552-906233, reverse complement, Verified ORF, "Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium" [yeast-contam.fasta] Cobalt uptake protein COT1 [nr-271106-contam.fasta] COT1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; Cot1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] orf 06131 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Cobalt uptake protein COT1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=COT1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] COT1 SGDID:S000005843, Chr XV from 907555-906236, Genome Release 64-1-1, reverse complement, Verified ORF, "Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium" [yeast-030211-contam.fasta] COT1 SGDID:S000005843, Chr XV from 907555-906236, Genome Release 64-2-1, reverse complement, Verified ORF, "Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YOR316C SGDID:S000005843, Chr XV from 907555-906236, Genome Release 64-1-1, reverse complement, Verified ORF, "Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	439 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ] fungal-type vacuole [IDA]
Biological Process:	cobalt ion transport [IMP] zinc ion transport [IGI] cellular zinc ion homeostasis [IMP]
Molecular Function:	cobalt ion transmembrane transporter activity [IMP] zinc ion transmembrane transporter activity [IGI] di-, tri-valent inorganic cation transmembrane transporter activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKLGSKQVKI ISLLLLDTVF FGIEITTGYL SHSLALIADS FHMLNDIISL VVALWAVNVA  60
   61 KNRNPDSTYT YGWKRAEILG ALINAVFLIA LCVSILIEAL QRIIAPPVIE NPKFVLYVGV 120
  121 AGLISNTVGL FLFHDNDQEH GHGHGHSHGG IFADHEMHMP SSHTHTHAHV DGIENTTPMD 180
  181 STDNISEIMP NAIVDSFMNE NTRLLTPENA SKTPSYSTSS HTIASGGNYT EHNKRKRSLN 240
  241 MHGVFLHVLG DALGNIGVML SAFFIWKTDY SWKYYTDPLV SLIITGIIFS SALPLSCKAS 300
  301 KILLQATPST LSGDQVEGDL LKIPGIIAIH DFHIWNLTES IFIASLHIQL DISPEQFTDL 360
  361 AKIVRSKLHR YGIHSATLQP EFITREVTST ERAGDSQGDH LQNDPLSLRP KTYGTGISGS 420
  421 TCLIDDAANC NTADCLEDH

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		BPT1 CCC1 COT1 CTR2 FTH1 VCX1 VMA9 ZRC1 ZRT3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	COT1	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
cation transmembrane transporter activity	6.20443686476571	bayes_pls_golite062009
ion transmembrane transporter activity	6.11479851651253	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	5.71521511150856	bayes_pls_golite062009
transporter activity	3.98293753989079	bayes_pls_golite062009
transmembrane transporter activity	3.88313530012213	bayes_pls_golite062009
substrate-specific transporter activity	3.86867537460555	bayes_pls_golite062009
cadmium ion transmembrane transporter activity	3.15489440737786	bayes_pls_golite062009
metal ion transmembrane transporter activity	2.61862894000527	bayes_pls_golite062009
transition metal ion transmembrane transporter activity	2.19121021485065	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	2.14914871177232	bayes_pls_golite062009
cation channel activity	1.78793567733454	bayes_pls_golite062009
efflux transmembrane transporter activity	1.41612086977895	bayes_pls_golite062009
binding	1.28505954375411	bayes_pls_golite062009
ion channel activity	1.24140857317889	bayes_pls_golite062009
di-, tri-valent inorganic cation transmembrane transporter activity	1.20401058605752	bayes_pls_golite062009
passive transmembrane transporter activity	1.19443885425411	bayes_pls_golite062009
channel activity	1.19443885425411	bayes_pls_golite062009
substrate-specific channel activity	1.19404477926718	bayes_pls_golite062009
active transmembrane transporter activity	0.475232131680683	bayes_pls_golite062009
protein binding	0.318355149027855	bayes_pls_golite062009
iron ion transmembrane transporter activity	0.186398867523746	bayes_pls_golite062009

Term	Confidence	Notes
cation transmembrane transporter activity	6.60087208506814	bayes_pls_golite062009
ion transmembrane transporter activity	6.49419690808578	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	6.066906606566	bayes_pls_golite062009
transporter activity	4.27173462293249	bayes_pls_golite062009
substrate-specific transporter activity	4.19993137135137	bayes_pls_golite062009
transmembrane transporter activity	4.18558383943492	bayes_pls_golite062009
cadmium ion transmembrane transporter activity	3.5658659940326	bayes_pls_golite062009
metal ion transmembrane transporter activity	2.68106285271045	bayes_pls_golite062009
transition metal ion transmembrane transporter activity	2.32644140472297	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	2.19343183150052	bayes_pls_golite062009
efflux transmembrane transporter activity	2.11491587250012	bayes_pls_golite062009
cation channel activity	1.89531256510168	bayes_pls_golite062009
ion channel activity	1.33777369753101	bayes_pls_golite062009
di-, tri-valent inorganic cation transmembrane transporter activity	1.31663166812163	bayes_pls_golite062009
substrate-specific channel activity	1.28221434614717	bayes_pls_golite062009
passive transmembrane transporter activity	1.27960450391272	bayes_pls_golite062009
channel activity	1.27960450391272	bayes_pls_golite062009
binding	1.27456652114636	bayes_pls_golite062009
active transmembrane transporter activity	0.490905558612378	bayes_pls_golite062009
iron ion transmembrane transporter activity	0.376948884172728	bayes_pls_golite062009
protein binding	0.311357063667067	bayes_pls_golite062009

Source: Reynolds et al. 2008. Manuscript submitted

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..439]	Pfam	67.568636	Cation efflux family No confident structure predictions are available.
2	View Details	[107..439]	PSI-BLAST	48.522879	No description for 2qfiA was found.

Protein Overview: COT1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: