Protein Overview: CHS1

General Information:
Name(s) found:	CHS1 / YNL192W [SGD]
Description(s) found: Found 20 descriptions. SHOW ALL	Chitin synthase I, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for repairing the chitin septum during cytokinesis; transcription activated by mating factor [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1131 amino acids
Gene Ontology:
Cellular Component:	chitosome [IDA] plasma membrane [IDA]
Biological Process:	cytokinesis, completion of separation [IMP] cell budding [TAS]
Molecular Function:	chitin synthase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDQNNRSRN EYHSNRKNEP SYELQNAHSG LFHSSNEELT NRNQRYTNQN ASMGSFTPVQ  60
   61 SLQFPEQSQQ TNMLYNGDDG NNNTINDNER DIYGGFVNHH RQRPPPATAE YNDVFNTNSQ 120
  121 QLPSEHQYNN VPSYPLPSIN VIQTTPELIH NGSQTMATPI ERPFFNENDY YYNNRNSRTS 180
  181 PSIASSSDGY ADQEARPILE QPNNNMNSGN IPQYHDQPFG YNNGYHGLQA KDYYDDPEGG 240
  241 YIDQRGDDYQ INSYLGRNGE MVDPYDYENS LRHMTPMERR EYLHDDSRPV NDGKEELDSV 300
  301 KSGYSHRDLG EYDKDDFSRD DEYDDLNTID KLQFQANGVP ASSSVSSIGS KESDIIVSND 360
  361 NLTANRALKR SGTEIRKFKL WNGNFVFDSP ISKTLLDQYA TTTENANTLP NEFKFMRYQA 420
  421 VTCEPNQLAE KNFTVRQLKY LTPRETELML VVTMYNEDHI LLGRTLKGIM DNVKYMVKKK 480
  481 NSSTWGPDAW KKIVVCIISD GRSKINERSL ALLSSLGCYQ DGFAKDEINE KKVAMHVYEH 540
  541 TTMINITNIS ESEVSLECNQ GTVPIQLLFC LKEQNQKKIN SHRWAFEGFA ELLRPNIVTL 600
  601 LDAGTMPGKD SIYQLWREFR NPNVGGACGE IRTDLGKRFV KLLNPLVASQ NFEYKMSNIL 660
  661 DKTTESNFGF ITVLPGAFSA YRFEAVRGQP LQKYFYGEIM ENEGFHFFSS NMYLAEDRIL 720
  721 CFEVVTKKNC NWILKYCRSS YASTDVPERV PEFILQRRRW LNGSFFASVY SFCHFYRVWS 780
  781 SGHNIGRKLL LTVEFFYLFF NTLISWFSLS SFFLFFRILT VSIALAYHSA FNVLSVIFLW 840
  841 LYGICTLSTF ILSLGNKPKS TEKFYVLTCV IFAVMMIYMI FCSIFMSVKS FQNILKNDTI 900
  901 SFEGLITTEA FRDIVISLGS TYCLYLISSI IYLQPWHMLT SFIQYILLSP SYINVLNIYA 960
  961 FCNVHDLSWG TKGAMANPLG KINTTEDGTF KMEVLVSSSE IQANYDKYLK VLNDFDPKSE1020
 1021 SRPTEPSYDE KKTGYYANVR SLVIIFWVIT NFIIVAVVLE TGGIADYIAM KSISTDDTLE1080
 1081 TAKKAEIPLM TSKASIYFNV ILWLVALSAL IRFIGCSIYM IVRFFKKVTF R

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2006)		CHS1 ENO2 PGK1
View Details	Ho Y, et al. (2002)		ARO1 BCK1 CHS1 CMP2 DBP7 KOG1 KSP1 PRI2 PSY2 TPD3
View Details	Qiu et al. (2008)		CHS1 CHS3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC34	CHS1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..142]	PSI-BLAST	2.69897	Fibrinogen
2	View Details	[143..409]	ORFEUS	14.32	RBP1
3	View Details	[410..488]	deduced	N/A	No confident structure predictions are available.
4	View Details	[489..565] [696..855]	ORFEUS	7.7	Diol dehydratase, alpha subunit
5	View Details	[566..695]	ORFEUS	7.7	Diol dehydratase, alpha subunit
6	View Details	[856..908]	deduced	N/A	No confident structure predictions are available.
7	View Details	[909..1131]	MSA	1.122952	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
transferase activity, transferring hexosyl groups	2.23446625487311	bayes_pls_golite062009
UDP-glycosyltransferase activity	1.84596125277114	bayes_pls_golite062009
catalytic activity	1.73979371332125	bayes_pls_golite062009
transferase activity	1.18156663897445	bayes_pls_golite062009
acetylgalactosaminyltransferase activity	0.823843077041128	bayes_pls_golite062009
cytidylyltransferase activity	0.666765511953956	bayes_pls_golite062009
nucleotidyltransferase activity	0.641710647713692	bayes_pls_golite062009
binding	0.61043334112269	bayes_pls_golite062009
polypeptide N-acetylgalactosaminyltransferase activity	0.600389101620522	bayes_pls_golite062009
transferase activity, transferring glycosyl groups	0.539883035270389	bayes_pls_golite062009
glucuronosyltransferase activity	0.510390802401046	bayes_pls_golite062009
mannosyltransferase activity	0.505948108814334	bayes_pls_golite062009
glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity	0.360354014290234	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.301396160525425	bayes_pls_golite062009
UDP-N-acetylglucosamine diphosphorylase activity	0.0309517682490415	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2