Protein Overview: TPO1

General Information:
Name(s) found:	TPO1 / YLL028W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74627235\|sp\|Q07824.1\|TPO1_YEAST RecName: Full=Polyamine transporter 1 [NCBI NR] gi\|6323000\|ref\|NP_013072.1\| Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane; Tpo1p [ [NCBI NR] pir\|\|S64779 probable membrane protein YLL028w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1360210\|emb\|CAA97477.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Polyamine transporter 1 OS=Saccharomyces cerevisiae GN=TPO1 PE=1 SV=1 [Swiss-Prot] TPO1 Vacuolar polyamine-H+ antiporter [mips] Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane [SGD] TPO1, Chr XII from 84803-86563 [yeast_orfs2] Chr XII from 84803-86563 [yeastorf3.fasta] >Q07824 (Q07824) Chromosome XII reading frame ORF YLL028W [4932.FASTACCERV.txt] TPO1 SGDID:S0003951, Chr XII from 84803-86563, Verified ORF [yeastflysequest.fasta] Proton-motive-force-dependent multidrug transporter of the major facilitator superfamily; able to transport eight different compounds, including polyamines, quinidine, cycloheximide, and nystatin; involved in excess spermidine detoxification [Saccharomyce [nrdb_11152004_Chlamydomonas] (Q07824) S.cerevisiae chromosome XII reading frame ORF YLL028w [cerv4932.FASTAC] TPO1 SGDID:S000003951, Chr XII from 84803-86563, Verified ORF, "Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sor [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane; Tpo1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] TPO1 SGDID:S000003951, Chr XII from 84803-86563, Verified ORF, "Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Polyamine transporter 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TPO1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] TPO1 SGDID:S000003951, Chr XII from 84804-86564, Genome Release 64-1-1, Verified ORF, "Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane" [yeast-030211-contam.fasta] TPO1 SGDID:S000003951, Chr XII from 84804-86564, Genome Release 64-2-1, Verified ORF, "Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane" [20141208_orf_trans.fasta] YLL028W SGDID:S000003951, Chr XII from 84804-86564, Genome Release 64-1-1, Verified ORF, "Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	586 amino acids
Gene Ontology:
Cellular Component:	cellular bud [IDA] vacuolar membrane [IMP] plasma membrane [IDA ]
Biological Process:	polyamine transport [IMP]
Molecular Function:	spermine transmembrane transporter activity [IDA][IMP] spermidine transmembrane transporter activity [IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDHSPISNK ENHLLPSDSS RSSSSDMHST GTTGTTGVEP VDFTGEGAKY TTATEGNGGA  60
   61 DLAIQRTTTM NSAAESEVNI TRRLTKILTG SVNEPDRVEV DYTNCAPMGG DRPYPPSLPS 120
  121 RDLYEVTFDG PNDPLHPFNW PMKKKVLLCL VLCLDSIAIA MCSSIFASAV PQICEIYHVI 180
  181 EVVAILGITL FVLGFAASPV IYAPLSELYG RKGVLVLSAF GFALFQFAVA TAENLQTIFI 240
  241 CRFFGGFIGA APMAVVPAAF ADMFDTNVRG KAIALFSLGV FVGPILSPVM GSYIAQRTTW 300
  301 RWLEYVVGCF ASAVFVAIVL FFEETHHPTI LVNKAKQMRK QSNNWGIHAA HEDVELSIKD 360
  361 IVQKTVTRPI IMLFVEPLLL FVTIYNSFVY GILYLLLEAY PLVFVEGYGF TENGELPYIA 420
  421 LIIGMMVCAA FIWYMDNDYL KRCRAKGKLV PEARLYAMVI AGTVFPIGIL WFCWTGYYPH 480
  481 KIHWMVPTVG GAFIGFGLMG IFLPCLNYII ESYLLLAASA VAANTFMRSA FGACFPLFAG 540
  541 YMFRGMGIGW AGLLLGLFAA AMIPVPLLFL KYGESIRKKS KYAYAA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		AGP2 AVT3 GAP1 TPO1 TPO2 TPO3 TPO4

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PSF1	TPO1	2	Hazbun TR, et al. (2003)
View Screen	RAD1	TPO1	2	Unpublished Fields Lab Data
View Screen	CLB2	TPO1	2	Unpublished Fields Lab Data
View Screen	YPT6	TPO1	2	Unpublished Fields Lab Data
View Screen	ARF1	TPO1	1	Unpublished Fields Lab Data
View Screen	SPO21	TPO1	1	Unpublished Fields Lab Data
View Screen	APC5	TPO1	1	Unpublished Fields Lab Data
View Screen	YDR049W	TPO1	1	Unpublished Fields Lab Data
View Screen	SWI5	TPO1	1	Unpublished Fields Lab Data
View Screen	YPR097W	TPO1	1	Unpublished Fields Lab Data
View Screen	PRP42	TPO1	1	Unpublished Fields Lab Data
View Screen	GLC7	TPO1	1	Unpublished Fields Lab Data
View Screen	SPT2	TPO1	1	Unpublished Fields Lab Data
View Screen	CAB2	TPO1	1	Unpublished Fields Lab Data
View Screen	YJU2	TPO1	1	Unpublished Fields Lab Data
View Screen	YLR140W	TPO1	1	Unpublished Fields Lab Data
View Screen	RED1	TPO1	1	Unpublished Fields Lab Data
View Screen	YKU80	TPO1	1	Unpublished Fields Lab Data
View Screen	SPT21	TPO1	1	Unpublished Fields Lab Data
View Screen	SPO1	TPO1	1	Unpublished Fields Lab Data
View Screen	KAP104	TPO1	1	Unpublished Fields Lab Data
View Screen	RHO5	TPO1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TPO1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..119]	deduced	N/A	No confident structure predictions are available.
2	View Details	[120..323] [365..586]	PSI-BLAST	19.30103	No description for 1ja5A_ was found.
3	View Details	[324..364]	PSI-BLAST	19.30103	No description for 1ja5A_ was found.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
transporter activity	4.09176719149278	bayes_pls_golite062009
substrate-specific transporter activity	3.59744164024479	bayes_pls_golite062009
transmembrane transporter activity	3.5865018981083	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	3.54194478414824	bayes_pls_golite062009
ion transmembrane transporter activity	2.21266150105753	bayes_pls_golite062009
cation transmembrane transporter activity	1.79369588808394	bayes_pls_golite062009
active transmembrane transporter activity	1.59419818573076	bayes_pls_golite062009
organic acid transmembrane transporter activity	1.24937104052238	bayes_pls_golite062009
secondary active transmembrane transporter activity	1.22264637452409	bayes_pls_golite062009
binding	1.01584233411372	bayes_pls_golite062009
alcohol transmembrane transporter activity	0.924836669821548	bayes_pls_golite062009
polyol transmembrane transporter activity	0.924836669821548	bayes_pls_golite062009
glycerol transmembrane transporter activity	0.812001619402678	bayes_pls_golite062009
symporter activity	0.74591237387473	bayes_pls_golite062009
solute:cation symporter activity	0.74591237387473	bayes_pls_golite062009
cation:sugar symporter activity	0.143419068761182	bayes_pls_golite062009
sugar:hydrogen symporter activity	0.143419068761182	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2